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Serologic and cross-immunity studies with contagious ecthyma and goat pox virus isolates from the western United States.

Contagious ecthyma and goat pox viruses were isolated from goats affected with papulopustular epidermal lesions. Results of in vitro serum neutralization and in vivo cross-immunity studies indicate that these contagious ecthyma and goat pox isolates from the western United States are antigenically dissimilar and that exposure or vaccination with one isolate could not be used as a method of inducing immunity to the other. Exposure to each isolate conferred immunity to re-exposure with the same agent. The serum neutralization test indicates that the severity and persistance of lesions to goat pox infection influences the nature of the antibody response to an initial exposure.

Animals

Contagious ecthyma in mountain goat of coastal British Columbia.

Contagious ecthyma has been reported previously from mountain goat (Oreamnos americanus) in one restricted area of eastern British Columbia. A second focus of infection is reported for mountain goat from western British Columbia. Diagnosis was based on appearance of lesions at necropsy, histopathology and demonstration of poxvirus with the electron microscope. The epizootiology of this infection in mountain goat is discussed briefly.

Animals

[Contagious ecthyma with virus demonstration in a negative staining method].

The diagnosis of contagious ecthyma was presumed by history and clinical appearance in a 33-year-old farmer. In electron miscroscopical preparations viral particles were found by the negative staining method. It is proposed to use this method for rapid diagnosis of suspected cases. In old lesions it is more difficult to demonstrate viral particles than in fresh lesions. Differential diagnosis and the position of Orf-virus in the system of viruses are briefly discussed.

Adult

[Properties of the contagious ecthyma virus used for vaccine production].

Experiments were carried out to establish the effect of some antibiotics and merthiolate on the vaccinal Phylaxia strain of ecthyma contagiosa. Besides, followed up was the effect of these antibiotics on concomitant microflora that had multipled in the virus suspension. Trials were also made to titrate the vaccinal strain on lambs through scarification. Titration was carried out by the method of Schmidt. Virus dilutions of 10-1 were applied following scarification on the the thighs and fore limbs of two lams. A scarified site was also left without application to check up the effect. Each treatment needed two lambs. It was found that the antibiotics nistatin, kanamycin, oxacillin, tetraolean, oleandomycin, oxitetracyclin and chloramphenicol did not lower the activity of the vaccinal virus Phylaxia. The antibiotics tested at the respective concentrations had no sterilizing effect on the crust media regard to the concomitant bacteria. It is pointed out that the method of virus treatment can be employed to determine the biologic potential of productional batches of ecthyma contagiosa vaccine.

Age Factors

ORF in sheep and goats in Nigeria.

Orf virus was demonstrated in biopsy material taken from lambs during an outbreak of the disease on the University of Ibadan Teaching and Research Farm. Clinical cases were also seen in goats. The confirmation of orf in Nigeria is discussed in relation to peste des petits ruminants, an important virus disease of small ruminants in West African countries.

Animals

Causation and epidemiological features of proliferative lip and facial lesions in Lao goats.

Past surveys and studies have identified a syndrome of lip and facial dermatitis as common in Lao goats and identified Orf virus (ORFV) as the likely cause. To more definitively determine the cause of this syndrome, a matched case-control study was implemented in 70 smallholder goat farms and a large-scale goat holding from Savannakhet province, Laos. Forty-one scab samples from goats with lip and facial dermatitis (cases) and 41 paired samples from unaffected animals (controls) were collected from 31 goat holdings over 12&#x2009;months. Samples were tested using quantitative PCR assays targeting possible pathogens causing such lesions. The prevalence of PCR-positive samples in case and control goats was 100% and 95.1%, respectively, for ORFV (P&#x2009;=&#x2009;0.15) and 19.5% and 29.3% (P&#x2009;=&#x2009;0.3) for Dermatophilus congolensis. Pseudocowpox virus and bovine popular stomatitis virus were not detected in any samples. There was a clear difference between the log10 genome copies (GC) per &#xb5;L DNA extract of ORFV in positive samples from cases (7.00&#x2009;&#xb1;&#x2009;0.17) and controls (2.62&#x2009;&#xb1;&#x2009;0.18, P&#x2009;<&#x2009;0.0001). This was not true for D. congolensis with similar GC detected in the case and control goats. More than 4 log higher GC in affected cases identifies ORFV as the likely cause of the syndrome in Lao goats with little role for D. congolensis. Phylogenetic analysis showed no genetic variation of ORFV in the study samples and close similarity to sequences from China. The findings suggest that orf disease has a sporadic distribution, mostly occurring in goats during the first 6-months of life.

Animals

Viral diseases of the skin, 1975: a 25-year perspective.

Most of the major advances in modern virology during the past 25 years have been due principally to the development of refined laboratory techniques and tools and have provided a fund of new knowledge and information about the nature of viral infection and pathogenesis. One group of viruses of interest to dermatologists, the herpesviruses, is undergoing intensive biochemical investigation to determine whether it is carcinogenic. As a result of the success of the World Health Organization's campaign to eradicate smallpox, it is predicted that by the end of 1976, smallpox will have been eradicated. Other viruses of dermatologic interest which are now being studied include the agents of warts, molluscum contagiosum, cat-scratch disease, and enteroviruses. Current research in the field of viral chemotherapy may provide the basis for successfully treating these diseases in the future.

Acrodermatitis

Orf: a family with unusual complications.

Three members of a farming family and their local postman contracted orf. One of those affected had had no direct contact with infected sheep. Two of the family developed a widespread papulo-vesicular eruption of the skin and mucosae with pyrexia, malaise and lymphadenopathy lasting 4-5 weeks. The eruption did not resemble erythema multiforme or the toxic erythemas usually associated with this infection.

Adolescent