Permanent marking of colored mice using dry ice.
Freeze marking, using dry ice, was successfully applied to colored mice for permanent, individual, identification. By this method, a 5-8 mm piece of dry ice is pressed against the skin of mice for 10 seconds. About 3 weeks after this treatment, spots of white hair growing at the site of treatment became recognizable to the naked eye, and allowed permanent identification of individual animals thereafter. The white spots were very clear in colored mice, and greatly facilitated animal identification as compared to conventional marking methods. Treatment of the animals with dry ice for 10 seconds has no discernible effects on the site of the treatment or the general condition of the animals.