[Experimental evolutive cycle of Diphyllobothrium erinaceieuropei in Paracyclops fimbriatus, tadpoles of Bufo arenarum and dogs].
Experiments were performed in order to develop the life cycle of Diphyllobothrium erinaceieuropei Rudolphi 1819 (Cestoda, Pseudophyllidea) in Paracyclops fimbriatus and Bufo arenarum as intermediate hosts and dogs as definitive hosts. The eggs of Diphyllobothrium erinaceieuropei from faeces of naturally infected dogs were kept refrigered, in water. In order to obtain coracidiums they were incubated at 25 degrees C, and then were placed in a flask which contained Paracyclops fimbriatus. The copepods were observed to be infected with procercoids 12 days after, (mean temperature 22.6 degrees C) and then, ten tadpoles of Bufo arenarum were put into the same flask. The tadpoles were examined 22, 23, 61 and 107 days later, finding plerocercoids in all them (mean temperature 24.9 degrees C). On the 23rd day, 49 plerocercoids were removed from 6 tadpoles and 28 of them were fed to a bitch. On the 107th day, 11 plerocercoids were recovered from a dead tadpole and 3 of them were fed to another bitch. In the faeces of the first bitch there were observed the eggs of Diphyllobothrium erinaceieuropei 22 days post infection (d.p.i.) and part of the strobila 30 d.p.i.. In the faeces of the second bitch the eggs were found 30 d.p.i..