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Cysticercus bovis: pinocytosis in the Cysticercus tegument.

The authors have found that pinocytosis occurs in the tegument of C. bovis from the fourth week after infection. Electron-lucid bladders surrounded by plasma membrane were encountered in the distal cytoplasm. The pinocytosis occurred in form of micropinocytotic bladders only in the bladder tegument but not in the scolex. The bladders appeared first in the superficial part of the distal cytoplasm. During the following periods of development of the larva they were dispersed in the whole distal cytoplasm and were found even in the processes of subtegumental cells and inside these cells near the heterolysosomes.

Animals

[Free amino acid components of cystic fluid of Cysticercus cellulosae].

The free amino acids in the cystic fluid of Cysticercus cellulosae were determined quantitatively by using automatic amino acid analyzer. The cystic fluids of both subcutaneous-muscular Cysticercus cellulosae from 14 pigs and cerebral Cysticercus cellulosae from 10 pigs were detected. Among the 18 amino acids detected, alanine was found to be the predominant amino acid in both of the cystic fluids. The quantities of glycine and proline were higher than other amino acids in subcutaneous-muscular Cysticercus cellulosae, while threonine was higher than the rest in the cerebral Cysticercus cellulosae. A comparison was made between the quantities of amino acids in the fluids of both subcutaneous-muscular and cerebral Cysticercus cellulosae of 7 pigs by the T-test method. The results showed that the quantities of 12 out of 15 amino acids were significantly different, with particular reference to threonine, alanine, serine, and valine, suggesting that the difference in the metabolism of amino acids in Cysticercus may be related to the parasite's location in their respective hosts.

Amino Acids

In vitro hydroxylation of proline in the collagen of the cysticercus of Taenia solium.

1. In vitro hydroxylation of proline in cysticercus collagen was carried out using two different vertebrate enzymes. 2. Chick embryo enzyme is more active on cysticercus collagen than new-born rabbit enzyme. 3. Hydroxylation of cysticercus collagen is more efficient at 40 than at 37 degrees C. 4. No prolyl hydroxylase activity was detected in cysticercus extracts. 5. Collagen from the adult tapeworms Taenia solium and T. saginata lack hydroxyproline. 6. SLS crystallites from T. solium and T. saginata collagen show the same band pattern as cysticercus collagen.

Animals

[Study on localization of cystic fluid antigen of Cysticercus cellulosae using McAb-IGSS].

A study was undertaken on the localization of specific combination of McAb against cystic fluid antigen of Cysticercus cellulosae by immunogold silver staining method (IGSS). It was shown that the combination sites of McAb F3 were distinct in the frozen and paraffin sections of Cysticercus cellulosae. The black granules in the bladder walls presented double-layer distribution and those in the scolex folded sites concentrated into pieces. The combination sites of McAb labeled by IGSS against cystic fluid antigen of Cysticercus cellulosae were consistent with the distribution of the cell layers of Cysticercus cellulosae. The sections in the control group had scarce and irregular distribution, which could be differentiated distinctly from those in the experimental group.

Animals

The viability of Cysticercus cellulosae in Thai native food (NHAM).

The purpose of this study was to determine the viability of Cysticercus cellulosae in various forms of Thai native food (Nham). Four duplicated experiments were carried out and represent as in the average values. The results revealed that under the room temperature (27-30 degrees C), the intact C. cellulosae in Nham could survive as long as 12-18 hours after preparation while isolated C. cellulosae and C. cellulosae in a piece of 2 X 9 cm. of pork in 0.85% saline could survive as long as 60 and 66 hours, respectively. The Cysticercus in other ingredients of Nham and in saline solution (6%) could be viable for 48-96 and 12-18 hours, respectively. Under the temperature of 4 degrees C, Cysticercus in various recipes could survive for 96 hours while in the controls, it could survive as long as 20-30 days. However, C. cellulosae in various compositions of Nham could be viable 11-20 days which was longer than those in potassium nitrate or salt alone. The findings of this study might infer the suggest that optimum time for consumming Nham in raw condition with safety should be after preparation at least 2-3 days at room temperature.

Cysticercus

Pulmonary cysticercus.

Cysticercus cellulosae, the encysted larva of Taenia solium, is reported in a solitary granulomatous lesion excised from the lung of a 61-year-old woman from Thailand. No extrapulmonary signs of cysticercosis were manifest preoperatively, and the diagnosis was not suspected clinically. Pulmonary cysticercus is rarely diagnosed histologically. When unaccompanied by extrapulmonary disease, a single cysticercus lung lesion may be clinically and radiologically indistinguishable from other granulomata and malignancy.

Animals

The successful removal of a subretinal cysticercus by pars plana vitrectomy.

A 39-year-old woman from Belize presented with recent vision loss in her left eye due to a subretinal cysticercus located near the macula. We are reporting the use of standard vitrectomy instrumentation and techniques to successfully remove the subretinal cysticercus by a transretinal approach. Pars plana vitrectomy with transretinal removal of the cysticercus is a useful alternative to the traditional external sclerotomy.

Adult

Tissue reaction in the heart of cattle with a spontaneous and artificial Cysticercus bovis infection.

Results of detailed studies on tissue reactions to Cysticercus bovis in the heart of cattle, together with a comparison of findings in animals with spontaneous and experimental infection, and an evaluation of tissue reactions in relation to the location, morphology and morphogenesis of C. bovis provided evidence for the fact that in general, the response of the heart to the presence of C. bovis was an inflammatory reaction characterized by the origin of a pseudoepithelial border and a zone of granulation tissue. Later, when necrotic changes started to affect the cysticercus, the inflammatory reaction started to develop anew. It was accompanied by an exudation and a subsequent resorption. Characteristic features were focal necroses both of the exudate and the inflammatory border followed by a dystrophic calcification, focal necroses and a dystrophic calcification of individual collagenous fibres and groups of these fibres in addition to a regular appearance of necrotic-like foci typical of a reaction to C. bovis. The location of the cysticercus in a certain part of the lymphatic system of the heart and in skeletal muscles was shown to have a considerable effect on the course of the inflammatory reaction. Differences in the development of the inflammatory reaction explained concomitant findings of dead and live cysticerci at the same time after an experimental infection.

Animals

Pars plana vitrectomy for removal of intravitreous Cysticercus.

Pars plana was used for the removal of an intravitreous cysticercus, with minimal postoperative inflammation and an excellent visual result. This approach avoids lens removal and the visualization provided by the operating microscope allows removal of all vitreous humor particles and debris. The aspirated specimens allowed identification of the Taenia solium cysticercus.

Adult

Cysticercus of 60-milliliter volume in human brain.

A cerebral cyst 5 cm in diameter and 60 ml in volume was found to be as large as a racemose cysticercus. Because this cyst bore necrotic remnants of a scolex and its hooks, it was presumed to be a cysticercus of Taenia solium. This case demonstrates that in contrast with the unusual presentation of cerebral cysticercosis, cysts of the Taenia solium type can be very large, and when such cyst is encountered, a special search should be made for evidence of a scolex.

Brain

Racemose cysticercus in human brain. A case report.

A case of invasion of the brain of a 48-year-old man, a native of Louisiana, by a larval tapeworm is described. Inasmuch as there were no scolices or rostellar hooks present the parasite was identified as a racemose cysticercus which could be either a sterile coenurus of Taenia multiceps (= Multiceps multiceps), Taenia serialis (= Multiceps serialis), or an aberrant cysticercus of Taenia solium or of a different species of Taenia. Most of the parasite was removed surgically with a preoperative diagnosis of brain tumor. After operation the patient improved, and 32 months after onset of his illness he was working and showed no evidence of a recurrence.

Brain

Morphological determination of the age of Cysticercus bovis in very young calves with cysticercosis.

Characteristics and measurements are given for early developmental stages of Cysticercus bovis. The data emerging from our study indicate that the age of a one to two month-old larva from a spontaneous infection can reliably be determined in histological sections. On these grounds we have been able to determine that the cysticercus infection in the two calves (aged 21 and 27 days respectively) had been acquired prenatally.

Animals

[Immunoenzymatic test for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid of patients with meningitis of unknown etiology].

The enzyme-linked-immunosorbent assay using a new type of support consisting of discs of synthetic fabric-resin (ELISA-d) with antigenic components of Cysticercus cellulosae covalently bound, was employed with the purpose of investigating the presence of specific antibodies to the cysticerci (neurocysticercosis) in the cerebrospinal fluid (CSF) of patients with meningitis without etiological agent determined by the conventional methods. The test was performed in 277 CSF samples (128 children and 149 adults). The mean of optical density values (OD) obtained for 22 CSF normal patients (the diagnostic hyphotesis of meningitis was discarded) was of 0.03. The 44 CSF of patients with meningitis caused by other agents but C. cellulosae, showed OD of 0.05. The cut off determined with these two groups (control group) was 0.13 (OD + 3 SD). The group of 13 CSF of neurocysticercotic meningitis presented OD of 0.41 (0.10 to 0.91). Among the 198 CSF samples of indetermined meningitis, 23 (11.6%) presented OD above the cut off, which suggests the possibility that Cysticercus cellulosae was the etiological agent in this meningitis episode. The ELISA-d test has proved to be efficient for the immunodiagnosis of neurocysticercosis when conducted on CSF samples. The alterations observed in the 23 CSF reactive on ELISA-d were: increased number of lymphomononuclear cells in five, of polymorphonuclear cells in 13 and both cells in five samples.

Adolescent

Immunization procedure-related immunoglobulin levels in the development of antibodies against Cysticercus cellulosae.

Various immunization procedures were investigated in an effort to improve the number of hybridomas producing antibodies against Cysticercus cellulosae. Ten groups of 5 BALB/c mice were subjected to different immunization procedures and were bled repeatedly over a period of 68 days. The samples of sera thus obtained were tested by enzyme linked immunosorbent assay: total immunoglobulins, IgG and IgM levels were determined. In general, total anticyst antibody titres increased during the course of immunization but in 3 groups the final titre was lower than the maximal antibody titre. Overall, immune tolerance did not appear to be a problem and longer immunization programs seemed to end with slightly higher antibody levels. So far, 4 mice from the group that exhibited the highest immunoglobulin levels have been used for hybridoma production. Out of 124 hybridomas thus obtained, only 1 secreted antibodies against Cysticercus cellulosae.

Animals

[Localization of Cysticercus ovis in experimentally infected lambs].

An experimental infection was induced in lambs with 1000, 4000, 7000, and 30000 T. ovis eggs. It was found for the first time that Cysticercus ovis could be established not only in the already known regions of the body, but also in the skin and the subcutaneous connective tissue. The lesions were followed up in the skin on the 10th, 20th, 30th, and 45th day following infection. Later on the cysticerci localized in the skin caused its total piercing. The skin portions above the cysticerci, localized in the subcutaneous tissue, proved unsuitable to treatment and handling and remained defective. Hides that were obtained from animals infected with Cysticercus ovis were shown to be discarded in the leather and fur industries.

Animals