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Comparative study of microorganisms and sensory attributes of condiments from the fermentation of different seeds.

Seeds of African locust bean, melon, castor oil bean and soybean were processed and fermented for 3 days to produce local condiments in the laboratory with a method that simulated the traditional production process. Microorganisms associated with their fermentation and the organoleptic properties of the products were compared. Altogether, seven species of bacteria were involved in the fermentation. These included five Bacillus spp. and one species each of Pseudomonas and Staphylococcus. Their occurrence vary between the seeds on the different days of fermentation. However, Bacillus spp. were present in all the seeds throughout the fermentation period. The sensory evaluation preference rating for the four products was highest for soybean condiment, followed by that made from locust bean. Melon condiment was the least preferred among the four products. Statistical analysis showed that there were significant differences (P less than or equal to 0.05) among the four products for each of the four organoleptic properties evaluated by the judges and also for titratable acidity. Glutamic acid level of soybean condiment was highest (0.31%) among the four products with that of melon being the lowest (0.04%). These results could serve as useful indices for the development of starter culture and optimization of production process in commercializing the production of these local condiments.

Bacillus

[Resistance of Toxoplasma gondii to sodium chloride and condiments in pork sausage].

The aim of this study was to determine the resistance of Toxoplasma gondii cysts to salt (sodium chloride) and condiments (black pepper and garlic) in fresh sausages prepared with experimentally infected pork. The sausages were treated with 1.25, 2.00, and 2.50% salt with condiments added, and were refrigerated for 2, 24, and 48 hours, after which they were artificially digested. Mice were then inoculated with the digested matter. After 42 days the surviving mice were sacrificed and their serum examined using indirect immunofluorescence to detect anti-T. gondii antibodies. This examination showed that the treatments with salt over periods of less than 24 hours did not eliminate the parasite, and that only after 48 hours did the salt, at concentrations of 2.00 and 2.50%, effectively kill the parasite. The results of this experiment also showed that the condiments had no effect on the viability of T. gondii.

Animals

Inhibitory effects of condiments and herbal drugs on the growth and toxin production of toxigenic fungi.

The effects of thirteen kinds of powdered herbal drugs and seven kinds of commercial dry condiments on the growth and toxin production of Aspergillus parasiticus, A. flavus, A. ochraceus, and A. versicolor were observed by introducing these substances into culture media for mycotoxin production. Of the twenty samples tested, cinnamon bark completely inhibited the fungal growth, while the others only inhibited the toxin production. The inhibitors were easily extracted from the samples with solvents such as hot water, chloroform, or ethanol. The extracts from coptis, philodendron bark, mustard, green tea leaves, and zanthoxylum completely inhibited the aflatoxin production of A. parasiticus, however, they had little or no inhibitory effect against A. flavus.

Aspergillus

Simple and accurate method for determination of ethylene chlorohydrin in dried spices and condiments.

A simple and accurate method is described for the determination of ethylene chlorohydrin (ECH) by using capillary gas chromatography (GC) and flame ionization detection. Acetonitrile-methanol was chosen as the extraction solvent in preference to other solvents because its use reduced the number of compounds detected by the GC system, thus enabling easier identification and quantitation of ECH. The coefficient of variation for the method is 2.7% at 5 ppm, and recovery is good, even for the standard addition of 1 ppm. Fifteen different spices and condiments were analyzed using this method; 20% were identified as positive for ECH. The method also identifies the related compound ethylene bromohydrin (EBH).

Chlorohydrins

Tyramine content of preserved and fermented foods or condiments of Far Eastern cuisine.

The tyramine content of foodstuffs typical of the Far East was analysed: the items included fermented food and condiments as well as seven menus from different Far Eastern restaurants. The results of the present analysis extend to Far Eastern foods our previous conclusion that no severe dietary restrictions are needed in patients treated with moclobemide, a novel RIMA anti-depressant.

Asia

Application of a new spectrophotometric method for zinc determination in beverages and condiments.

A new spectrophotometric method has been applied to the determination of zinc in bottled mineral water (with and without gas), refreshments, vinegar, and table salt. The method introduces the 1-[di-(2-pyridyl)-methylidene]-5-(salicylidene)-thiocarbo hydrazone (DPST) as chromogenic reagent. This procedure is simple, rapid, reproducible and does not demand costly outfit and chemicals.

Beverages

Mycoflora and vitamin content of sun-dried food condiments in Nigeria.

The fungi most frequently isolated (of 20-100% incidence) from fresh okra, pepper fruits and melon seeds are Botryodiplodia theobromae, Rhizopus stolonifer, Trichoderma harzianum, Mucor mucedo and Fusarium oxysporum. The major mycoflora of the fruits which had been sun-dried for twenty days are comprised of Aspergillus spp and Penicillium spp which initially had formed the minor components (of less than 20% incidence) of the colonising population. The effect of sun-drying on the fruits was a statistically significant (P = 0.05) decrease in the riboflavin content and a generally small and non-significant decrease in the concentrations of thiamine, niacin, biotin and ascorbic acid. The effect of mouldiness during sun-drying was to further decrease the concentration of the vitamins.

Aspergillus

[The celery-carrot-mugwort-condiment syndrome: skin test and RAST results].

Celery is a frequent food allergen: not only raw, but also cooked and as a spice it can produce various reactions of immediate type, from oral contact urticaria to anaphylactic shock. Most celery-allergic patients suffer from hay fever and show a skin sensitization to mugwort. An associated allergy to several spices is quite common, and therefore the term "celery-mugwort-spice-syndrome" has been proposed. The authors have investigated 35 patients, 85% of them women, in whom a current celery allergy of varying severity was diagnosed during the first 8 months of 1984. A positive skin test to celery was seen in 16 patients who denied any symptoms from it. Thorough skin testing and RAST screening with different celery preparations, other foodstuffs and mugwort-pollens were performed on all patients. The modified prick test with native celery-root proved to be the best method for detecting celery sensitization, showing a positive result in 88.6%. The scratch test with celery-salt was positive in 70.5%, intracutaneous testing with commercial extract in 63.5% and the RAST with celery-sticks in 66% of the patients. Sensitization to mugwort was absent in 8 patients only. The mugwort-sensitive group frequently showed a positive test to ragweed-pollens, a common plant in North America, and this appears to indicate cross-reactivity amongst the family of the Asteraceae. Cross-reactivity amongst the Apiaceae is the cause of the many positive results obtained with carrot, parsely, anise, fennel and caraway, the carrot allergy being of clinical importance in 50% of cases, including one with a history of anaphylactic shock after ingestion of raw carrots.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult

Condiments.

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Condiments