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At least 19 recordsLinked to original sources
PubMed2026

Intra-individual genetic diversity of vaginal Lactobacillus crispatus revealed through citizen science-driven isolation and pangenome analysis.

AIMS: A vaginal microbiome dominated by Lactobacillus crispatus is associated with positive reproductive and sexual health outcomes, yet intra-individual genetic diversity within this species remains largely unexplored. This study characterized inter- and intra-individual genomic variation in L. crispatus strains isolated through a citizen science initiative and assessed implications for multi-strain probiotic development. METHODS AND RESULTS: Fifty-three women participated in this citizen science project. Self-sampling resulted in 48 shotgun metagenomes. Twenty-two participants isolated their own L. crispatus strains using selective enrichment and LAMP-based species confirmation, resulting in 53 whole-genome-sequenced isolates. Lactobacillus crispatus dominated 20 of 48 metagenomes (50.1%-99.6% relative abundance). Pangenome analysis revealed 3456 gene families, of which 43.7% were core and 56.3% accessory. A 14-kb plasmid harbouring a Fic-domain toxin-antitoxin protein, but devoid of antimicrobial resistance genes, was present in 44 of 53 strains. Strains from the same individual clustered closely together yet harboured 1-123 gene differences. Intra-individual variation was observed in the pullulanase type I gene required for glycogen degradation: 40 strains were predicted to grow on glycogen, six showed genetic disruptions with unknown consequences, and seven were predicted to lack this ability entirely. Variation within individuals was also found for bacteriocin classes and CRISPR-Cas genes. CONCLUSIONS: Substantial functional diversity exists within L. crispatus, even among strains from the same individual, supporting the rationale for multi-strain vaginal probiotics. This citizen science approach enabled discovery of host-specific adaptations while ensuring participant ownership of their strains.

Female↗
PubMed2026

Fantastic microbes and where to find them: evaluating learning-by-doing outcomes in a crowdfunded metagenomics workshop.

Metagenomics offers a powerful framework for authentic, interdisciplinary learning, yet it remains underrepresented in undergraduate education due to technical and infrastructural barriers. We hypothesized that a research-based, learning-by-doing metagenomics workshop supported by accessible bioinformatics tools could enhance students' perceived skills, self-efficacy, and conceptual understanding of metagenomic analysis. To test this hypothesis, we designed and evaluated a hybrid hands-on workshop in which undergraduate and postgraduate students analyzed real environmental shotgun metagenomic datasets generated from soil samples collected during a citizen science initiative. Using the graphical workflow platform KBase, participants completed an end-to-end metagenomic analysis, from quality control and assembly to genome reconstruction, taxonomic classification, functional annotation, and scientific presentation of results. Educational outcomes were assessed through validated retrospective pre-post questionnaires, self-efficacy scales, and an open-ended conceptual understanding task. Participants showed significant increases in perceived metagenomic skills and confidence in performing metagenomic analyses, while gains in perceived learning showed a positive trend. Conceptual understanding improved across educational levels, particularly among participants with limited prior experience. Together, these findings demonstrate that authentic, data-driven metagenomics activities can effectively lower barriers to computational biology and foster meaningful learning through hands-on research experiences.

Metagenomics↗
PubMed2016

DNA barcoding in diverse educational settings: five case studies.

Despite 250 years of modern taxonomy, there remains a large biodiversity knowledge gap. Most species remain unknown to science. DNA barcoding can help address this gap and has been used in a variety of educational contexts to incorporate original research into school curricula and informal education programmes. A growing body of evidence suggests that actively conducting research increases student engagement and retention in science. We describe case studies in five different educational settings in Canada and the USA: a programme for primary and secondary school students (ages 5-18), a year-long professional development programme for secondary school teachers, projects embedding this research into courses in a post-secondary 2-year institution and a degree-granting university, and a citizen science project. We argue that these projects are successful because the scientific content is authentic and compelling, DNA barcoding is conceptually and technically straightforward, the workflow is adaptable to a variety of situations, and online tools exist that allow participants to contribute high-quality data to the international research effort. Evidence of success includes the broad adoption of these programmes and assessment results demonstrating that participants are gaining both knowledge and confidence. There are exciting opportunities for coordination among educational projects in the future.This article is part of the themed issue 'From DNA barcodes to biomes'.

Biodiversity↗
PubMed2026

Species and strain sharing in the vaginal microbiome of mothers and their adult daughters.

The vaginal microbiome is key for women's health. However, its establishment, interindividual variation and dynamics remain poorly understood. Here, we investigate bacterial relatedness at species and strain level in adult mother-daughter pairs from the large-scale citizen-science program Isala. Using metagenomic sequencing with quality control including 16S rRNA profile comparison, along with targeted culturing, we assess intergenerational microbiome sharing. At species level, daughters' vaginal microbiomes are significantly more similar to their mothers' than to those of unrelated mothers, with a strong mother-daughter correlation in Lactobacillus crispatus dominance. Strain-level analyses of metagenomes and isolate genomes reveal intraspecies diversity in L. crispatus, with up to two strains observed within the same host, and support intergenerational vaginal bacteria sharing. SNV counts in shared L. crispatus strains show no correlation with daughters' ages. Together, these findings suggest that maternal transmission, host factors, and (shared) environment collectively shape the vaginal microbiome, providing fundamental ecological insights into vaginal microbiome dynamics and perspectives toward lactobacilli-based applications.

CP: microbiology↗
PubMed2026

Beauty bias in butterfly research and conservation.

Conservation biases have been documented since the first emergence of the concept of biodiversity in the 1980s,1,2,3 showing a systematic disproportion in the allocation of research and conservation efforts among taxa.4,5,6,7,8,9,10,11 One factor underlying this disproportion, gaining prominence in recent literature, is species' perceived beauty, shaped by human visual preferences.12,13,14,15,16,17 Here, we integrate a large-scale survey of the perceived beauty of European butterflies yielding >21,000 survey completions from >100 countries into a time-explicit network linking species' beauty, public attention, research and conservation efforts, and the EU regulatory framework. We found that species beauty is consistently associated with public attention, research, and conservation efforts in a temporally structured pattern compatible with a cumulative beauty bias. Research effort and public attention concentrate on widespread and visually attractive species, whereas species included in the legal conservation framework, particularly the Convention on the Conservation of European Wildlife and Natural Habitats (hereafter, Bern Convention, BC, 1979)18 and the EU Habitats Directive (hereafter, HD, 1992)19 are disproportionately represented by visually appealing and historically protected taxa. Because these frameworks guide funding and management actions, early associations between species beauty and BC/HD inclusion have contributed to long-lasting institutional patterns in butterfly research and conservation. By contrast, European IUCN Red Lists20,21 do not overrepresent beautiful species and identify more inconspicuous taxa as threatened. This mismatch reveals a tension between scientific assessments of extinction risk and historically embedded conservation priorities. Our findings suggest that recognizing beauty bias is vital for aligning conservation with actual ecological urgency. VIDEO ABSTRACT.

Animals↗
PubMed2026

Analysis of the genome of a Pseudomonas monsensis isolate that produces the antifungal dipeptide maculosin.

Candida species have been attributed to causing ~647,000 deaths annually. Candida albicans has been identified by the WHO as a priority pathogen for targeted antifungal drug development. To this end, we have screened microbial strains from the public outreach project Swab and Send for anti-Candida activity. This process identified Pseudomonas sp. SS1954.14, which displayed potent activity against C. albicans, both on agar and in a cell-free supernatant assay. Whole-genome sequence analysis identified this strain as Pseudomonas monsensis. It contains several biosynthetic gene clusters, suggesting that it can produce hydrogen cyanide, lokisin, pyoverdine and colicin/carocin, which may contribute to the observed antifungal activity. However, an active compound was separated by preparative high-performance liquid chromatography and identified through high-resolution mass spectrometry as maculosin [cyclo(Pro-Tyr)]. This cyclic dipeptide has previously been found to possess antifungal activity, but the exact biosynthetic mechanism remains undetermined. Reporting this genome alongside the associated evidence of maculosin production represents a valuable resource for biosynthetic investigations.

Candida albicans↗
PubMed1991

The Foreign Medical Graduate Examination in the Medical Sciences (FMGEMS). An analysis of pass rates of the July 1984 through July 1987 examinations.

This report examines the experience of 48,509 (40,393 foreign nationals and 8,116 U.S. citizens) foreign medical students and graduates (FMGs) who took any part of the first seven Foreign Medical Graduate Examination in the Medical Sciences (FMGEMS) administered by the Educational Commission for Foreign Medical Graduates. The pass rate on all three sections (basic science, clinical science, and English) was 23.0%. Almost one-half (47.2%) of all FMGEMS examinees were between 25 and 29 years of age, 73.9% were men, 71.5% took FMGEMS after medical school graduation, and 79.7% claimed as their native tongue languages other than English. Correlates of pass rates included taking FMGEMS before medical school graduation (30.0% pass) and being a native English speaker (37.5% pass). The rates for foreign national FMGs (FNFMGs) and United States FMGs (USFMGs) were 22.5% and 25.2%, respectively. Native English-speaking FNFMGs achieved a 43.3% pass rate; native English-speaking USFMGs, 32.6%; non-native English-speaking FNFMGs, 19.9%; and non-native English-speaking USFMGs, 11.2%. Whereas FMGs were educated in 114 countries, 74.2% of USFMGs were educated in just eight countries, all located in the West Indies and Mexico. Logistical regression analysis showed that the strongest factors predicting full pass rates were being both younger than 30 years of age and a native English speaker. Conclusions are that approximately 3,200 FMGs per year pass FMGEMS and that FMGs with the highest probability of passing share characteristics of U.S. and Canadian medical graduates who pass the National Board medical examinations, which suggests that the latter examinations, when offered to FMGs, may have limited effect on overall pass rates.

Achievement↗
PubMed1979

Accountability in health care: an essay on mechanisms, muddles, and mires.

This paper presents a critical evaluation of several theories of accountability and their applicability to health care concerns. The authors first provide a preliminary refinement of the imprecise concept of accountability itself and then examine four major types (political; bureaucratic; professional; and economic-consumer). Then, by using disciplinary perspectives provided by several schools of thought in political science (legislative supremacy; general manager theory; government by bureaucracy; objective responsibility; citizen participation), they discuss the degree of accountability presently found in various American health policies. After identifying the inherent dilemmmas within any credible accountability approach to health care programs as now utilized in the United States, the authors recommend that all types of accountability be integrated and strengthened by relying more explicitly on practices characteristic of development administration.

Delivery of Health Care↗
PubMed1991

Population behavior change: a theory-based approach.

Behavioral science provides useful ideas about how programs of mass communication and community participation can produce synergistic influences on the lifestyles and policy decisions of populations and their representatives. Media campaigns featuring real-life behavior models and community networks mobilized to promote behavior change provide a theoretically sound paradigm for community-level activities to accelerate the diffusion of innovation in health. Illustrative case studies demonstrate how behavioral science concepts can be applied to preventing HIV infections and to promoting citizen lobbying against selected nuclear weapons systems.

Health Behavior↗
PubMedDate not supplied

An analysis of the historical context of liberal education in nursing education from 1924 to 1939.

This study examines in historical context the liberal education theme in nursing education as expressed in nursing literature from 1924 to 1939. The specific questions addressed are: What is the nature of liberal education as presented in the literature of the time? What did nurse educators believe would be the effect of the liberal education of the nurse on the profession of nursing? To what extent did the articulation of beliefs regarding liberal education parallel the development of nursing education at the collegiate level? The data include literature published in the major nursing journals, the proceedings of the national professional nursing organizations, and the major studies of nursing by various committees. The literature was examined for central themes. The study concludes that during this period, nurse educators believed the purposes of a liberal education for nurses to be the development of the individual and the citizen, the acquisition of cultural knowledge, the development of critical thinking, and the possession of a strong science background. A nurse with these abilities would be able to meet the changing demands of practice at a professional level. The college and university continued to be viewed as the optimum site for this education.

Curriculum↗
PubMed1975

Consumer evaluation of health services: implications for methodology and health care policy.

Consumer evaluation research is defined and analyzed as an experimental model for establishing a link between social science research and public policy. The paper reports the results of an 18-month longitudinal study of an experimental citizen evaluation group. Participant observation was the method used to organize the data. The impact of the model on methodological issues, such as objectivity, researcher autonomy, and control over research decision-making are discussed. Implications of the consumer evaluation model for integrating social research and health policy formulation are analyzed and principles of citizen evaluation research are presented.

Consumer Behavior↗
PubMed1975

Vocational potential assessment.

Determination of employment potential will become increasingly important in the foreseeable future, largely due to pending Federal legislation which relates to welfare reform. The heretofore "permanently and totally disabled versus able-bodied" principle in welfare reforms is being abbandoned. Pending legislative proposals dealing with welfare reform provide for considering physically impaired persons as partially disabled and partially employable simultaneously. Thus, the need to systematically and effectively assess physically impaired citizens' capacities to participate in the job market will increase. Unquestionably, rehabilitation medicine in general, and the emerging art and science of vocational evaluation in particular, will contribute much to supplying these services. While it is widely acknowledged that the vocational potential of physically impaired persons should be evaluated in an organized manner, there are differences of opinion among professional evaluators as to which approach, or approaches, are the most meritorious; The four principal approaches are: (1) mental testing, (2) work sampling, (3) situational analysis, and (4) job tryouts. Each of these approaches is explained, contrasted, and evaluated in this paper.

Disability Evaluation↗
PubMed1992

Narrowing the zone of uncertainty between research and development in biological warfare defense.

Although "research" is not prohibited by the Biological Weapons Convention, States Parties to the Convention have maintained the spirit of the Convention in actions relating to research. The confidence-building measures agreed to at RC2 refer to research facilities, publication of research results, and promotion of contacts between scientists engaged in research related to the Convention. However, assessment of basic research on biological agents is not a productive way to distinguish an offensive from a defensive program. Additionally, if a country were to initiate a biological weapons program, basic research on biological agents may not be necessary. For example, the extensive published research on Bacillus anthracis, both as a cause of anthrax in cattle and other species and as a biological-warfare agent, would enable any motivated group or nation to initiate a biological weapons program that could immediately advance to the development and scale-up stages. Research on biological agents for offensive purposes would be characterized by activities such as selection for growth, virulence, and toxin production; improving stability under varying environmental conditions; and selection of strains that might overcome existing means of prophylaxis and treatment. A biological program with an offensive intent would in most cases be characterized by evidence of development efforts in mass production and dissemination, which are often agent-specific. Thus, an assessment of development may distinguish offensive from defensive programs. If a country were to initiate a biological weapons research program, and were willing to risk worldwide condemnation should existence of such a program become known, it is likely that such a program would include development and production capabilities. If a country were not committed to production capability, there would be no rationale for an offensive biological research that would bring worldwide condemnation. Critics of the U.S. Biological Defense Research Program have suggested that the program could easily and quickly be turned into an offensive effort. To accomplish this, however, we have to assume that all military personnel, including the civilians employed by the Department of the Army, are unethical and willing to break the law and run the risk of placing the U.S. in a noncompliance status. The Army is under constant scrutiny by governmental agencies, by visiting scientists, by audiences at scientific meetings, by scientists who review manuscripts for publications, by news media, and by private citizens.(ABSTRACT TRUNCATED AT 400 WORDS)

Biological Warfare↗
PubMed1989

The determinants of food choices of the elderly.

The increase in individuals over 60 years of age in America must cause professionals from the fields of nutrition, food science, food systems management and the food industry to consider the determinants of food choices and the effects on the overall health and well-being of elderly citizens and those soon to be in their mature years. Income, household composition, food preferences, time, technology, education, good health, attitudes, physiological changes in vision, hearing, taste and smell, psychological and sociological factors all affect the food choices of the elderly.

Aged↗