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At least 19 recordsLinked to original sources
PubMed1985

When is a chrysanthemum dermatitis not a chrysanthemum dermatitis? The case for describing florists' chrysanthemums as Dendranthema cultivars.

In order to provide a new reference point in the dermatological literature from which the naming of florists' chrysanthemums may be regularised and standardised, the case is presented for the use of the generic name Dendranthema together with a cultivar name in place of a specific epithet. A review of cultivar specificity in chrysanthemum dermatitis is also presented.

Dermatitis, Contact↗
PubMed2004

Elimination of chrysanthemum stunt viroid from an infected chrysanthemum cultivar by shoot regeneration from a leaf primordium-free shoot apical meristem dome attached to a root tip.

In this research we eliminated chrysanthemum stunt viroid (CSVd) from a highly infected chrysanthemum cultivar using a newly established method. 'Piato' is one of the most difficult cultivars in which to obtain CSVd-free plants by conventional methods. Leaf primordium-free shoot apical meristems (LP-free SAMs) of 'Piato' plants were dissected and attached to CSVd-free chrysanthemum or cabbage root tips. As shown by nested-PCR, CSVd was not detected in some shoots regenerated on both types of root tip. The production rates of CSVd-free plants using chrysanthemum and cabbage root tips were 14% and 3%, respectively. Regeneration of plants from LP-free SAMs of chrysanthemum plants by attaching these SAMs to root tips is an efficient method of generating CSVd-free chrysanthemum plants.

Brassica↗
PubMed2004

Accumulation of pathogenesis-related type-5 like proteins in phytoplasma-infected garland chrysanthemum Chrysanthemum coronarium.

Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of pathogenesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.

Amino Acid Sequence↗
PubMed2003

Purification and characterization of polyphenol oxidase from garland chrysanthemum (Chrysanthemum coronarium L.).

Polyphenol oxidase (PPO) of garland chrysanthemum (Chrysanthemum coronarium L.) was purified approximately 32-fold with a recovery rate of 16% by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic chromatography, and gel filtration. The purified enzyme appeared as a single band on PAGE and SDS-PAGE. The molecular weight of the enzyme was estimated to be about 47000 and 45000 by gel filtration and SDS-PAGE, respectively. The purified enzyme quickly oxidized chlorogenic acid and (-)-epicatechin. The K(m) value (Michaelis constant) of the enzyme was 2.0 mM for chlorogenic acid (pH 4.0, 30 degrees C) and 10.0 mM for (-)-epicatechin (pH 8.0, 40 degrees C). The optimum pH was 4.0 for chlorogenic acid oxidase (ChO) and 8.0 for (-)-epicatechin oxidase (EpO). In the pH range from 5 to 11, their activities were quite stable at 5 degrees C for 22 h. The optimum temperatures of ChO and EpO activities were 30 and 40 degrees C, respectively. Both activities were stable at up to 50 degrees C after heat treatment for 30 min. The purified enzyme was strongly inhibited by l-ascorbic acid and l-cysteine at 1 mM.

Catechin↗
PubMed2001

Antifungal activity of the essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens.

The antifungal activity of Chrysamthemum coronarium was evaluated against 12 agricultural pathogens. Flowerhead oil was active both in contact and headspace in vitro assays producing hyphal growth inhibition, although there was less activity on faster growing fungi. The main compounds identified in the oil were camphor (29.2%), alpha-pinene (14.8%), beta-pinene (9.5%) and lyratyl acetate (9.8%). The blue color of the oil was due to the presence of chamazulene (0.5%).

Antifungal Agents↗
PubMed2002

Prevalence of occupational allergy to Chrysanthemum pollen in greenhouses in the Netherlands.

BACKGROUND: An increasing number of allergic complaints appear to have occurred among Chrysanthemum greenhouse employees. The aim of this study was to estimate the prevalence of work-related allergic symptoms and the prevalence of sensitization to pollen of different members of the Chrysanthemum family. METHODS: We studied 104 employees who were invited to answer an extensive questionnaire and to complete a rhinitis quality of life questionnaire. In addition, they were skin prick tested on location with inhalant allergens and home-made pollen extracts of seven different members of the Chrysanthemum family. Radio-allergo-sorbent tests were performed to confirm IgE-mediated reactions. RESULTS: Work-related symptoms were reported in 56.7% of all cases, with the main symptom being rhinitis. Sensitization to Chrysanthemum pollen was found in 20.2% of the employees without one member of the Chrysanthemum family in particular being most prevalent. Sensitization to Chrysanthemum pollen was considered to be an important risk factor for the occurrence of work-related symptoms of the upper airways. Furthermore, inhalant atopy as well as sensitization to common airborne pollen including mugwort were closely associated with sensitization to Chrysanthemum what might be suggestive for cross-sensitization. CONCLUSIONS: There is a high prevalence of work-related symptoms in Chrysanthemum greenhouses. In one-third of the employees these symptoms were caused by an IgE-mediated allergy caused by the pollen of the flowers. Inhalant atopy appeared to have a great impact on the development of such a sensitization. Measurements to reduce the pollen exposure are necessary to prevent a further increase of this occupational allergy.

Adolescent↗
PubMed1975

Chrysanthemum allergy. Pt. II: Experimental studies on the causative agents.

Experimental studies on allergic contact dermatitis due to Chrysanthemum indicum 1. (Chrysanthemum x-morifolium) (chrysanthemum of the florists) have indicated the following results: 1. In 5 patients with allergic contact dermatitis from chrysanthemum oil of turpentine and its sensitizing compounds gave no patch test responses. No relationship between contact allergy due to chrysanthemus and to turpentine oil could be determined. 2. The pyrethrins, constituents with insecticidal activity, derived from certain Chrysanthemum species and often suspected as the causative agents, play no role in chrysanthemum allergy. 3. Tests on sensitized guinea pigs (pirl bright white strain) with flowers of chrysanthemum as well as with the two sesquiterpene lactones parthenolide and alantolactone, derived from different Composite species, gave positive patch test reactions. The results showed that parthenolide produced stronger reactions than alantolactone. 4. By thin layer chromatography neither parthenolide, nor alantolactone or pyrethrosin could be detected in extracts of chrysanthemum flowers of the florists. But the investigations indicated that several other terpenic compounds are present, which gave positive color reactions to certain lactone reagents. Five of them showed strong positive patch test reactions in our patients as well as in sensitized guinea pigs. Further studies are required to identify these compounds.

Adjuvants, Immunologic↗
PubMed2003

Using the expolinear growth equation for modelling crop growth in year-round cut chrysanthemum.

The aim of this study was to predict crop growth of year-round cut chrysanthemum (Chrysanthemum morifolium Ramat.) based on an empirical model of potential crop growth rate as a function of daily incident photosynthetically active radiation (PAR, MJ m-2 d-1), using generalized estimated parameters of the expolinear growth equation. For development of the model, chrysanthemum crops were grown in four experiments at different plant densities (32, 48, 64 and 80 plants m-2), during different seasons (planting in January, May-June and September) and under different light regimes [natural light, shading to 66 and 43 % of natural light, and supplementary assimilation light (ASS, 40-48 micro mol m-2 s-1)]. The expolinear growth equation as a function of time (EXPOT) or as a function of incident PAR integral (EXPOPAR) effectively described periodically measured total dry mass of shoot (R2 > 0.98). However, growth parameter estimates for the fitted EXPOPAR were more suitable as they were not correlated to each other. Coefficients of EXPOPAR characterized the relative growth rate per incident PAR integral [rm,i (MJ m-2)-1] and light use efficiency (LUE, g MJ-1) at closed canopy. In all four experiments, no interaction effects between treatments on crop growth parameters were found. rm,i and LUE were not different between ASS and natural light treatments, but were increased significantly when light levels were reduced by shading in the summer experiments. There was no consistent effect of plant density on growth parameters. rm,i and LUE showed hyperbolic relationships to average daily incident PAR averaged over 10-d periods after planting (rm,i) or before final harvest (LUE). Based on those relationships, maximum relative growth rate (rm, g g-1 d-1) and maximum crop growth rate (cm, g m-2 d-1) were described successfully by rectangular hyperbolic relationships to daily incident PAR. In model validation, total dry mass of shoot (Wshoot, g m-2) simulated over time was in good agreement with measured ones in three independent experiments, using daily incident PAR and leaf area index as inputs. Based on these results, it is concluded that the expolinear growth equation is a useful tool for quantifying cut chrysanthemum growth parameters and comparing growth parameter values between different treatments, especially when light is the growth-limiting factor. Under controlled environmental conditions the regression model worked satisfactorily, hence the model may be applied as a simple tool for understanding crop growth behaviour under seasonal variation in daily light integral, and for planning cropping systems of year-round cut chrysanthemum. However, further research on leaf area development in cut chrysanthemum is required to advance chrysanthemum crop growth prediction.

Chrysanthemum↗
PubMed1994

Pattern of cross-sensitivity between 4 Compositae plants, Parthenium hysterophorus, Xanthium strumarium, Helianthus annuus and Chrysanthemum coronarium, in Indian patients.

To assess the pattern of cross-sensitivity between 4 members of the Compositae family, namely Parthenium hysterophorus L., Xanthium strumarium L., Helanthus annuus L. and Chrysanthemum coronarium L., 63 patients clinically diagnosed to have airborne contact dermatitis, and 51 controls having well-defined patterns of contact dermatitis caused by agents other than plants, were patch tested with measured amounts of standardized aqueous extracts of these plants. Positive reactions were obtained in 62 patients and 13 controls with Parthenium hysterophorus, in 47 patients and 9 controls with Xanthium strumarium, in 7 patients and 2 controls with Helianthus annuus, and in 13 of the 57 patients and one out of 28 controls tested with Chrysanthemum coronarium. 2 patients were allergic to all 4 of the plants; 14 patients to 3 plants, namely Parthenium, Xanthium and Chrysanthemum in 9 cases and Parthenium, Xanthium and Helianthus in 5 cases; 32 patients to 2 plants, namely Parthenium and Xanthium in 30 cases, and Parthenium and Chrysanthemum, and Xanthium and Chrysanthemum in 1 case each; 15 patients were allergic to 1 plant only, that being Parthenium. All the 47 patients allergic to Xanthium, 13 patients allergic to Chrysanthemum and 7 patients allergic to Helianthus were positive with some other plant as well. There was 1 patient who was allergic to Xanthium and Chrysanthemum but not to Parthenium. The titre of contact hypersensitivity (TCH) determined in the patients allergic to Parthenium, Xanthium and Helianthus showed values that varied widely with each plant in different patients, and there was no parallelism between the TCH with various plants.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed2002

[Effect of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells]

OBJECTIVE: To observe the effect and the mechanism of Chrysanthemum morifolium Ramat on apoptosis of bovine aortic smooth muscle cells. METHODS: Vascular smooth muscle cells were isolated from thoracic aorta of fetal calf and cultured, then incubated with different concentration of Chrysanthemum morifolium Ramat. Apoptosis was measured by flow cytometry. SOD and MDA were measured by spectrophotometer. RESULTS: We found that: (1) the number of apoptotic cells was reduced from (4.425+/-0.624)% to (2.875+/-0.640)% in Chrysanthemum morifolium Ramat group, in a concentration dependent manner; (2) the value of SOD was increased from (1.683+/-0.149)X10(4) U/L to (2.297+/-0.230)X104 U/L and the value of MDA was reduced from(166.454+/-56.805)&mgr;mol/L to (73.068+/-27.203)&mgr;mol/L in Chrysanthemum morifolium Ramat group, also in a concentration dependent manner. CONCLUSION: Chrysanthemum morifolium Ramat can inhibit apoptosis of vascular smooth muscle cells in a concentration-dependent manner.

Journal Article↗
PubMed1987

Occupational dermatitis with simultaneous immediate and delayed allergy to chrysanthemum.

A 42-year-old woman had recurrent dermatitis on the hands, arms, face and trunk for 5 years. She was allergic to chrysanthemum, with positive patch tests. She also had contact urticaria to chrysanthemum, with a wheal-and-flare reaction 15 min after application of chrysanthemum juice to a scratch test site. This was not seen in control subjects. The co-existence of allergic contact dermatitis and contact urticaria from chrysanthemum might play a rôle in the development of dermatitis, especially severe chronic dermatitis of her hands.

Adult↗