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Results for “CROSS INFECTION”

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At least 19 recordsLinked to original sources

Salmonella enterica: infection, cross infection and persistence within the environment of a broiler parent stock unit in Denmark.

A total of three hundred and ninety nine isolates of Salmonella enterica were recovered from 3106 cloacal swab cultures, chicken carcasses and environmental samples taken from a broiler breeding farm over six successive generations. S. enterica serovars Berta (S. Berta), 4,12:b:-, Taksony, Binza and Typhimurium were identified. One hundred and seventy one strains of S. Berta were analysed by plasmid profiling to determine the epidemiological relationships between isolates from each generation and from each house section of the production unit. All isolates contained plasmids; plasmids of eleven different molecular weights were identified. Eighteen different plasmid profiles were identified throughout the observation period. An increase in the diversity of plasmid profiles in each successive generation, the restriction of particular plasmids to particular houses and their persistence within that house were taken to indicate that contamination within the unit was due to persistence and cross-infection rather than to a reintroduction of serovar Berta into new generations of stock.

Animals↗

Infection and cross-infection in a paediatric gastro-enteritis unit.

A two month study to investigate the incidence of nosocomial infection was conducted in a paediatric gastroenteritis ward of a black academic hospital. Enteric pathogens were identified on admission in 61 (47.2%) of 129 patients; 56 bacterial and 25 viral. Six per cent of patients had a combination of bacterial and viral pathogens. Enteric pathogens most frequently identified on admission were Campylobacter jejuni in 22%. Rotavirus in 19.3%, EPEC in 10.8% and Shigella spp. in 6.9% patients. Twenty six (20%) patients had more than 1 enteric pathogen. The nosocomial infection rate was recorded at 17.1%. EPEC occurred most commonly in 5.3% patients, Salmonella typhimurium in 4.6% and Shigella spp. in 2.3%. Nosocomial infections increased the mean length of hospital stay from 7.2-20.2 days. Contributory factors to the spread of nosocomial infection were the unsatisfactory methods of bathing patients and giving naso-gastric feeds.

Academic Medical Centers↗

CROSS-INFECTION.

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Cross Infection↗

Indirect calorimetry in patients with active respiratory infection--prevention of cross-infection.

BACKGROUND: Measurement of resting energy expenditure in patients with active respiratory infection is hampered by the risk of contamination of indirect calorimeters and connecting tubes that cannot be sterilized. This validation study tested whether the use of disposable standard-bore air tubes and an antibacterial filter, by reducing air flow, introduces an error due to recirculation of expired air in the canopy. METHODS: Eleven healthy volunteers underwent indirect calorimetry twice in random order, using a Deltatrac calorimeter either with standard wide-bore tubing or with a disposable standard-bore air tube and filter. Methods were compared by Bland-Altman plots and by calculating trends over time. RESULTS: The new tube and filter reduced air flow by 40%. Measured resting energy expenditure did not differ significantly between methods, with limits of agreement -135 to +188 kcal/d. Carbon dioxide flow (VCO(2)) and respiratory quotient (RQ), but not oxygen flow decreased slowly over time with both methods. CONCLUSIONS: The use of an air filter and standard-bore tubes do not introduce a systematic error into indirect calorimetry. Although trends in VCO(2) and RQ are consistent with minor recirculation of exhaled air, this modified Deltatrac system can be safely and reliably used to measure resting energy expenditures in patients with active tuberculosis and other airborne infection.

Adult↗

Virus cross-infection in paediatric wards.

In a survey of virus cross-infection in paediatric wards there were 15 cross-infections due to respiratory syncytial (R.S.) virus and 16 due to influenza A, both during a four-month period, and 19 due to parainfluenza viruses over two years. The illnesses produced by these infections acquired in hospital ranged from a slight cold to severe pneumonia: in 17 of the 50 cases the illness involved the lower respiratory tract. A measure of cross-infection frequency in the form of a "cross-infection rate" has been devised, and it is suggested that this can be used to assess the influence of factors such as ward design and admission policy on the frequency of cross-infection.

Age Factors↗

Determination of the viremia threshold for dental cross-infection in a mouse model.

An animal model of dental virus transmission was developed using the lactate dehydrogenase-elevating virus (LDV) of mice to study cross infection. Mouse-to-mouse cross-infection was carried out by scaling the teeth of LDV-infected donor mice with dental instruments, immediately prior to using the contaminated instruments on the teeth of recipient indicator mice. The level of donor viremia was found to correlate with the rate of virus cross-infection, with a viremia threshold level of 10(7.5) ID50/ml observed for dental cross-infection. The blood volume transferred during dental cross-infection was approximately 10(-4) to 10(-5) ml, demonstrating the inefficiency of virus cross-infection, since deposition of about 1000 virions on dental instruments was associated with the threshold limit. Virus transferred during dental cross-infection rapidly entered the blood circulation, showing that dental cross-infection was not dependent on an oral infection. The results from these model studies predict the general inefficiency of dental instrument virus cross-infection, and a further reduced likelihood of dental cross-infection with appropriately cleaned instruments.

Animals↗

Analysis of cross infection using genomic fingerprinting in nosocomial urinary tract infection caused by Enterococcus faecalis.

To evaluate cross infection and a possible outbreak of Enterococcus faecalis urinary tract infection (UTI) in our urology ward, we studied the DNA fingerprinting of E. faecalis strains isolated from nosocomial UTI patients, in the period 1982-1996, using arbitrarily primed polymerase chain reaction (AP-PCR) analysis. The serovar and amplified products of DNA extracted from clinically isolated urinary E. faecalis strains by the AP-PCR method were analyzed, and the respective isolation periods of E. faecalis-positive UTI patients were investigated. There were nine patients with E. faecalis UTI between March and May 1994 and all strains isolated from their urine specimens were serovar type 7. AP-PCR revealed that five of the nine isolates had the same pattern. It appeared that these strains had caused the outbreak of E. faecalis UTI. Cross-infection between patients with E. faecalis UTI was demonstrated by genomic fingerprinting, suggesting that cross infection had occurred via urinary catheters or by hand contact in our ward. We may, therefore, reasonably conclude that we should beware of the transmission of urinary E. faecalis and take countermeasures against its dissemination.

Journal Article↗

Outbreak of Clostridium difficile diarrhoea in an orthopaedic unit: evidence by phage-typing for cross-infection.

In a three-week period five patients had diarrhoea in an orthopaedic unit. The first case was clinically diagnosed as pseudomembranous colitis but the causative agent was not sought. Of the remaining cases, two were Clostridium difficile positive. The outbreak then apparently ceased, but during the following several days two of seven stool samples taken at random from asymptomatic patients yielded C. difficile. Phage-typing of the isolates showed that all apparently belonged to the same strain.

Adolescent↗