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At least 19 recordsLinked to original sources

[Attachment of horse cecum Ciliata to plant fragments. Degradation of chloroplasts. Attachment of bacteria to cecal Ciliata].

Cecum microfauna association with different plant tissues was examined by scanning and transmission electron microscopy. The ciliates were attached to the damaged areas of the leaves and their highest concentrations were found on the epidermis and mesophyll tissues. The degradation of plant tissue was due to protozoal ingestion of the plant fragments. The morphology of ingested envacuolized chloroplasts changed rapidly, showing different stages of digestion inside the ciliate endoplasm. Intact chloroplasts were rarely observed but the grana of fragmented thylakoid membranes was often seen. The chloroplasts then appeared in concentric rings resembling pseudomyelinic figures. Plastoglobuli indicated the chloroplastic origin of these figures. Cecum microflora association with the microfauna showed that the different morphological types of cecal bacteria had three ways of attaching: some appeared to be attached via small amounts of extracellular material and some by capsule - like material; others (bacilli), due to an electron-dense outer layer, were observed to adhere so closely that they conformed to the shape of the ciliate.

Animals↗

Anti-inflammatory and analgesic properties of the stem bark extract of Mitragyna ciliata (Rubiaceae) Aubrév. & Pellegr.

Mitragyna ciliata is widely used in traditional medicine for the treatment of inflammation, hypertension, headache, rheumatism, gonorrhoea and broncho-pulmonary diseases. In the present study, the anti-inflammatory and analgesic properties of the stem bark extract of M. ciliata were investigated. The stem bark of this plant was extracted over Soxhlet with hexane followed by another extraction with methanol. The resulting methanol extract was used for the pharmacological test. Anti-inflammatory activity was evaluated on the basis of the inhibitory effect of the extract on 5-lipoxygenase, and carrageenin-induced hind paw oedema in the rat. The methanol extract, at a dose of 19.2 microg/ml, exhibited no inhibition on 5-lipoxygenase. However, this extract administered per os (50 mg/kg) produced about 70% inhibition of carrageenin-induced paw oedema 1 h after administration. This inhibition was maintained to about 50% 2 h after administration. The dose of 50 mg/kg of MeOH extract significantly decreased sensitivity to pain from 78.75 to 107.5 g These findings suggest that extracts of the bark of M. ciliata, possess potent anti-inflammatory and analgesic effects. Chemical analysis of the extract showed the presence of alkaloids and kaempferol derivative which may be responsible for the anti-inflammatory properties.

Analgesics↗

The effects of ciguatoxin on the nerves of the teleost fish, Sillago ciliata.

The absolute refractory period, relative refractory period, and the duration and magnitude of the supernormal period were measured after incubation of fish nerves with ciguatoxin and other channel modifying compounds, tetrodotoxin, veratridine, verapamil, and lignocaine. In vitro electrophysiological studies were carried out on the lateral line nerve of the whiting, Sillago ciliata Cuvier. Electrophysiological changes in fish nerves after exposure to ciguatoxin (0.3 MU.ml-1) and veratridine (1 x 10(-5) M) are similar to changes that occur in mammalian nerves and include an increase in the absolute refractory period, the relative refractory period, and the magnitude and duration of supernormality. The effects of ciguatoxin (0.3 MU.ml-1) in fish nerves were antagonised by tetrodotoxin (5 x 10(-10) M), verapamil (5 x 10(-7) M), and lignocaine (1 x 10(-5) g/ml-1). The nerves of Sillago ciliata used in this study responded to ciguatoxin and its antagonists in a similar manner to mammalian nerves, suggesting that these teleost nerves have no specific electrophysiological mechanism to cope with this toxin.

Animals↗

Antimicrobial activity of Toona ciliata and Amoora rohituka.

The extracts of two Bangladeshi medicinal plants, Toona ciliata (stem bark) and Amoora rohituka (stem bark), along with siderin, a major coumarin from T. ciliata, exhibited significant in vitro antibacterial activity. The extracts also demonstrated mild antifungal effect.

Anti-Infective Agents↗

Evaluation of anti-inflammatory potential of Bergenia ciliata Sternb. rhizome extract in rats.

The methanol extract of the rhizome of Bergenia ciliata Sternb. (Saxifragaceae) has been evaluated for anti-inflammatory potential using two acute rat models (carrageenan- and serotonin (5-HT)-induced rat paw oedema) and a chronic rat model (cotton pouch-induced granuloma). Phenylbutazone (100 mg kg(-1)), a non-steroidal anti-inflammatory agent, was used as a standard. The methanol extract (100, 200 or 300 mg kg(-1)) exhibited significant (P < 0.05) anti-inflammatory activity in all the animal models. At 300 mg kg(-1) the methanol extract exhibited maximum inhibition of 32.4+/-2.89% in carrageenan-induced rat paw oedema while the standard showed an inhibition of 44.1+/-2.7% after 3 h of drug treatment. In the serotonin-induced rat paw oedema model, 300 mg kg(-1) methanol extract suppressed oedema by 45.33+/-2.09%, whereas the standard produced an inhibition of 53.5+/-4.3%. In the cotton pouch granuloma model the methanol extract inhibited significantly (P < 0.001) the granuloma weight in a dose-dependent manner. In this model, 300 mg kg(-1) extract produced a maximum inhibition of 31.4+/-1.09% in granuloma weight compared with 41.1+/-1.32% reduction in granuloma weight for the standard. The methanol extract of B. ciliata exhibited significant anti-inflammatory potential at the dose levels examined.

Animals↗

[Effect of entomopathogenic fungus inoculum on the control of Corythycha ciliata Say adults, wintering on plane-trees of city groves].

Within a three years research program, infection tests were carried on adults of Corythucha ciliata wintering on the plane-trees of same city avenues, inoculating entomopathogenic deuteromycetes Beauveria bassiana, Verticillium lecanii, Paecilomyces farinsus, microorganisms which are known to be naturally present in such an environment. Inoculated fungi were able to settle only where the trees were free from any kind of disturbance, while this failed to occur in the areas of intense car traffic. Number of treatments efficiently affected pathogens diffusion in the trees. The persistency of the parasites on the insects, even one year after the treatment, seems to indicate the possibility of their use for the limitation of C. ciliata.

Animals↗

Secondary connections of the dorsal and ventral facial lobes in a teleost fish, the rockling (Ciliata mustela).

In the rockling, Ciliata mustela (Teleostei), a portion of the dorsal fin is a specialized chemosensory organ possessing solitary chemoreceptor cells innervated by a recurrent branch of the facial nerve. Previous studies have demonstrated that the specialized solitary chemoreceptor cell system is represented in the dorsal segment of the medullary facial lobe (DFL), whereas the taste buds in the remainder of the facial-nerve-innervated skin are represented in the ventral division of the lobe (VFL). The carbocyanine dye DiI was used to investigate the secondary and higher order brain connections of these two distinct subdivisions of the facial lobe. Both segments of the facial lobe sent fibers into the contralateral DFL via a dorsocaudal facial commissure and to the contralateral vagal lobes and VFL via fibers arching ventrally through the reticular formation. Ascending fibers from both facial lobe segments were traced into the secondary gustatory nucleus and into the lateral superficial facial nucleus, a small area in the dorsolateral brainstem laterally adjacent to the nucleus medialis of the octavolateral complex. Additionally, the VFL had reciprocal connections with a newly described nucleus adjacent to the incoming facial nerve root. Both DFL and VFL had descending fibers reaching two portions of the funicular nuclear complex, although the VFL contribution to this area is far more extensive than the DFL input. Thus, substantial overlap exists in the connections of the two facial subsystems; i.e., the solitary chemoreceptor information is not processed in nuclei distinct from those making up the usual gustatory lemniscus.

Animals↗

12alpha-hydroxystigmast-4-en-3-one: a new bioactive steroid from Toona ciliata (Meliaceae).

In a bioassay guided phytochemical investigation of Toona ciliata (Fam. Meliaceae), a new hydroxy steroidal ketone, 12alpha-hydroxystigmast-4-en-3-one (1) was isolated from the petroleum ether extract of the plant together with two known steroids and three C-methyl coumarins. The structure of 1 was established by means of spectroscopic analyses. Compound 1 was found cytotoxic in a brine shrimp lethality bioassay with LC50 of 9.9 microg/ml and it also showed significant antitumor activity with Ti50 value of 14.1 microg/ml in a potato disc bioassay.

Animals↗

Spinal and facial innervation of the skin in the gadid fish Ciliata mustela (Teleostei).

The pattern of innervation of the skin of the rockling Ciliata mustela was investigated to sort out spinal from facial nerve innervation of cutaneous chemosensory and mechanosensory systems. This fish has a variety of appendages with different functional sensory specializations, i.e., the chin barbel, pelvic fin, anterior dorsal fin, and dorsal trunk skin. The carbocyanine dye, diI, was applied to nerve stumps in dissected aldehyde-fixed tissue. In the case of the chin barbel, the dye was applied to both the trigeminal and facial nerve components. In the other cases, the dye was applied either selectively to the spinal nerves, to the facial nerves, or to both components. In the chin barbel, diI labeled nerve fibers associated with taste buds (TBs) and solitary chemosensory cells (SCCs) as well as relatively blunt free nerve endings, which closely approach the epidermal surface. In the pelvic fin, anterior dorsal fin, and dorsal trunk skin, taste buds, solitary chemosensory cells, and their innervation were labeled only after diI was applied to the facial nerve stumps. Application of diI to spinal nerves labeled delicate, free nerve endings and nerve fibers associated with small cells deep in the epidermis with features characteristic of Merkel cells. Transmission electron microscopy supports these results; after denervation of the facial component of the anterior dorsal fin, synaptic contacts with Merkel cells remained intact, whereas the synapses with the SCCs vanished.

Animals↗

The presence of two populations of sensory-type cells in the pineal organ of the five-bearded rockling, Ciliata mustela L. (Teleostei).

The pineal organ of the five-bearded rockling, Ciliata mustela L., was examined by means of electron microscopy. Two categories of sensory cells are described: 1) Sensory cells 1 (or photoreceptor cells sensu stricto) showing the characteristic ultrastructure of photoreceptor cells with a well-developed receptor pole (outer segment) and a transmitter pole (ribbon-type synapse in the basal pedicle contacting dendritic processes), and a segmental organization of organelles. 2) Sensory cells 2 (or photoneuroendocrine cells) displaying no particular segmentation. The ultrastructure of the receptor pole (outer segment) is variable in shape (with either long or short disks) and in the number of disks; some outer segments are simple cilia of the 9 + 0 type. This second cell category is rich in smooth endoplasmic reticulum, beta-particles of glycogen, dense inclusions of variable size and content, and dense-core vesicles 130 nm in diameter. These cells have an extended contact area with the perivascular space. The functional significance of both cell categories is discussed in terms of the known physiological responses of the pineal organ. A possible confusion in identification of interstitial cells and neuroendocrine cells in some teleost species is discussed.

Animals↗

On the ultrastructure and permeability of taste buds of the marine teleost Ciliata mustela.

The abundant taste buds of the barbels and free fin rays of the five bearded rockling, Ciliata mustela contain an average of 100-150 cells, falling into two types. Tubule-containing cells ('t-cells'), tentatively identified as receptor cells, and each surrounded by fibril-containing cells ('f-cells') in the central part of the bud. t-Cells also occur in two concentric shells separated by indifferent epithelial cells at the periphery of the bud. f-Cells are characterized by their concentrations of fine fibrils, and by granules or vesicles of 180-190 mmu diameter. The 100 or so receptor cells in a taste bud are innervated by some 250 axons. Lanthanum penetrates more deeply into the extracellular space of taste buds than into the extracellular space of the general epithelium, perhaps indicating that a greater area than the mere protruding tip of receptor cells may be accessible to chemical stimulation. Degenerating cells may provide an important route of entry for such external agents.

Animals↗

New lignan glycosides with potent antiinflammatory effect, isolated from Justicia ciliata.

Two new lignan glycosides, 4-O-[alpha-L-arabinopyranosyl-(1' "-->2' ')-beta-D-xylopyranosyl-(1' " '-->5' ')-beta-D-apiofuranosyl]diphyllin (1), named ciliatoside A (1), and 4-O-¿[beta-D-apiofuranosyl-(1' " "-->3' ")-alpha-L-arabinopyranosyl-(1' "-->2' ')][beta-D-xylopyranosyl-(1' " '-->5' ')]-beta-D-apiofuranosyl¿diphyllin (2), named ciliatoside B (2), were isolated from the whole plant of Justicia ciliata. The structures of 1 and 2 were determined by spectral and chemical methods. Compounds 1 and 2 strongly inhibited the accumulation of NO(2)(-) in lipopolysaccharide-stimulated RAW 264.7 cells in a concentration-dependent manner with IC(50) values of 27.1 +/- 1.6 and 29.4 +/- 1.4 microM, respectively.

Animals↗

Cytotoxic lignans of Justicia ciliata.

Two new naturally occurring 1-aryl-2,3-naphthalide lignans, cilinaphthalide A (1) and cilinaphthalide B (2), and nine known compounds were isolated from the whole plant of Justicia ciliata. Their structures were established by spectral analysis, and their cytotoxic activity was evaluated against several different cell lines. The known compound, justicidin A, showed potent cytotoxic effects against T-24, CaSki, SiHa, HT-3, PLC/PRF/5, and 212 cells in vitro.

Antineoplastic Agents, Phytogenic↗

Evaluation of anti-tussive activity of Bergenia ciliata Sternb. rhizome extract in mice.

The methanol extract of the rhizome of Bergenia ciliata Sternb. (Saxifragaceae) has been evaluated for its potential in a cough model induced by sulphur dioxide gas in mice. The extract exhibited significant anti-tussive activity in a dose-dependent manner, as compared with control. The antitussive activity of the extract was comparable to that of codeine phosphate (10 mg/kg body wt.), a standard anti-tussive agent. The extract at doses of 100, 200 and 300 mg/kg body wt. (p.o.) showed significant inhibition of cough reflex by 28.7, 33.9 and 44.2%, respectively, within 90 min of the experiment.

Administration, Oral↗

[Microsporidians, cnidosporidians and ciliatae (Peritricha: Peritrichida: Mobilina), found in fishes from water bodies of the Minusinsk region].

During the period of 20 years (1964-1984) 10,000 fish specimens collected in water bodies of the Minusinsk depression have been examined by the methods of total and partial parasitological dissections. The studies were held in the Enisey, Abakan, Tasheba, and Tuba rivers, in the Krasnoyarskoye and Sayano-Shushenskoye reservoirs, and in some lakes. The data on a parasite species composition of microsporidians, cnidosporidians and ciliatae are given.

Animals↗

[Chemical constituents of Girardinia palmata ssp ciliata].

Thirteen constituents were identified from Girardinia palmata ssp ciliata C. J. Chen by using GC-MS, which amounted to 84.5% of the total peaks, the main constituent were linoleic acid (52.01%) and dimethyl fumarate (10.05%). The dissociated amino acids and KNO(3) in the sample amounted to 43.30 mg/100 g and 3.0%. Five compounds were isolated and elucidated as octadecane, hexadecylic acid, beta-sitosterol, p-coumaric acid and potasium nitrate.

Alkanes↗

[Chemical components of Lindernia ciliata (Colsm) Pennell].

Three known compounds were isolated from the petroleum ether extract of the whole plant of Lindernia ciliata. They are beta-sitosterol, stigmasterol and lup-20(29)-en-3 beta-ol. Their structures were established by IR, MS, 1HNMR and 13CNMR spectroscopy.

Drugs, Chinese Herbal↗