Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Biuret Reaction”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 19 recordsLinked to original sources
PubMed1979

[Sensitive method for continuous detection of peptides and proteins using the biuret reaction and a copper-Sephadex reactor (author's transl)].

We describe a detection method relying both on the copper displacement from a Sephadex gel by peptides and proteins, and on the subsequent colorimetric determination of the complexed copper. The system described is fully automated and it permits a continuous analysis of column effluents. The choice of cuprizone as a detecting reagent for copper, enables one to bring the detection limit down to 200 ng for albumin and 60 ng for alanylglycylglycin. The specificity of the method is the same as the biuret reaction. Some examples of the possible applications are given.

Biuret Reaction↗
PubMed1991

[The determination of protein content of milk, cheese, and meat with the use of the biuret reaction].

A review is given of the different composition of biuret test solutions and experiments with the estimation of protein content of milk, cheese and meat by means of the biuret method are reported. The pure protein and the casein content of skimmed milk and the protein content of whole milk could be determined with biuret test solutions containing potassium hydroxide and a detergent. The use of the biuret method is advantageous in the case of cheese and meat, which are dissolved in potash lye or alkaline detergent solutions. Disturbances of the biuret method caused by lactose, fat or turbidity were eliminated after addition of hydrogen peroxide, by means of extraction or of additional measurements with a copper-free, zinc-containing biuret reagent. Deviations of the color intensity of biuret complexes were compensated by inclusion of standards in the measurement series.

Animals↗