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At least 19 recordsLinked to original sources
PubMedDate not supplied

[Correlation of skin test to grass pollen with basophil degranulation test results in 72 patients (author's transl)].

Seventy-two subjects have been tested by skin-test (ST) and human basophil degranulation test (HBDT) with grass pollen extract (GPE). HBDT was done, using a suspension enriched in basophils, by a technic on slide. Results are expressed by an index (DI) which is percentage of basophils having apparently disappeared (i. e. degranulated) in presence of GPE. No less than 100 intact basophils (and often many more) were always counted in absence of GPE (control) for each subject, so that DI higher than 35% are significant (p less than 0,001). DI were higher than 35% in 28 positive ST subjects for at less one of three dilutions of GPE (10(-4), 10(-6), 10(-8)); in the 5 subjects with false-negative result, 3 had a weak ST response. In 39 subjects with negative ST, DI were higher than 35% in two subjects and only for one dilution (10(-4)). So, concordance of results of HBDT and obviously positive ST reaches 94%. In 33 positive ST subjects, a significant correlation appears between DI (at 10(-4) and 10(-6)) and flare diameter and between DI (at 10(-4)) and wheals diameter. HBDT appears to be a suitable and simple technic for routinely study of reaginic allergies.

Basophils↗
PubMed1984

[Diagnostic value of the human basophil degranulation test in schistosomiasis].

The human basophil degranulation test (HBDT) was applied to the diagnosis of schistosomiasis. In 120 cases confirmed by parasitological examination, the test was shown to be extremely sensitive (90% clearly positive responses) and specific. The total immunoglobulin E levels were higher in infested subjects (average value: 2,000 IU/ml) compared to an average of 500 IU/ml in control subjects. However, wide individual variations were observed and other parasitoses can also produce considerable increases. The measurement of specific IgE showed a clear response with high positive levels in patients with bilharziasis whereas the response was always negative in the controls.

Adult↗
PubMed1990

[The use of the direct basophil degranulation test for the diagnosis of allergy].

Basophil degranulation test (BDT) was used in examinations of patients suffering from allergies. A modified technique was employed with toluidine blue staining and basophil scintillation in a counter chamber. The examinees were divided into three groups. Group 1 (n = 34) consisted of patients suffering from atopic bronchial asthma with suspected sensitization to home dust. Group 2 included 118 patients with a history of allergy to penicillin. Group 3 consisted of 51 patients with drug allergies (streptomycin, vitamin B1, procaine, analgin intolerance). In all the cases specific diagnosis was confirmed by skin and provocation test. 71.4 percent of the patients with sensitization to home dust allergen showed positive results in BDT, as did 68.6 percent of Group 3 subjects. Of the cases with penicillin sensitization confirmed by application test 91.7 percent were positive in BDT, of those with positive intradermal tests, 66.7 percent were BDT-positive, this being probably explained by individual sensitivity of the patients. These results recommend BDT, an informative and simple technique, for wide practice.

Asthma↗
PubMed1979

[Immunological investigations of house-dust and house-dust mites. Part III. A simplified basophil degranulation test. Preliminary communication (author's transl)].

The basophil degranulation test (BDT) after Shelley has been simplified and tried on patients allergic to house-dust or to house-dust mites. All 20 patients reacted to the mite extracts and 19 out of 20 to the house dust extracts. Out of 20 control persons 17 had negative results to the mite extract and 16 to the house-dust extract. The advantages of this new simplified method are discussed.

Basophils↗
PubMed1995

[Human basophil degranulation test (HBDT) in fungal allergic asthmatic patients].

The authors recommand the methodology of HBDT for testing fungal allergens, pH 7.6, 37 degrees C and 10 mg/ml are the most suitable experimental conditions. The positive rate of HBDT in allergic asthmatic patients is 91.7%, and correlated very well with the results of skin tests. The degranulation index (DI) in allergic asthma is significantly higher than that in normals (P < 0.001). The DI after one year's desensitization treatment with fungal allergens is markedly lowered than before (P < 0.001). The results suggest that HBDT is a simple, reliable and precise method to investigate allergens in vitro and also reflects the function of basophils in asthma.

Alternaria↗
PubMed1987

[Basophil degranulation test in suspected mould allergy].

Mould allergy is often linked with a pattern of signs and symptoms of the upper airways, especially of the nose. Since exact aetiopathological correlations are hard to determine by anamnesis only, further allergy diagnosis is very important. This often yields marked differences between intracutaneous and nasal provocation tests. The human basophil degranulation test (HBDT) now offers a further method of in-vitro diagnosis. This test can explore the condition of the basophils, which are of great importance for the allergic reaction besides the mast cells, and the ability of these cells to degranulate in the presence of a corresponding allergen. We investigated the correlations between the results of HBDT, intracutaneous skin test and nasal provocation test. 30 patients with perennial rhinitis suspected of being caused by moulds were tested using 4 mould mixtures. We found many more positive results with the nasal test and HBDT than with the skin test. The corrected contingency coefficient after Pearson showed a stronger correlation of HBDT to the incracutaneous skin test and to the nasal provocation test than between the two in-vivo methods. This is thought to be due to a wider sensitivity spectrum of the degranulation test which can possibly also measure other allergy reactions than type I after Commbs and Gell. We consider HBDT to be a valuable additional tool in allergy diagnosis. We should welcome a wider range of different allergenic test slides and a standardisation of allergenic extracts.

Adolescent↗
PubMed1980

[Use of the basophil degranulation test and the leukocyte migration inhibition test to characterize mycogenic sensitization].

The results of experiments in using the basophil degranulation test and the leukocyte migration inhibition test for the characterization of mycogenous sensitization are presented. The model of mycogenous sensitization was created in guinea pigs by 3 injections of homogenized Candida albicans cells. Immediate allergic reaction is shown to develop earlier, as indicated by the results of the determination of homocytotropic antibody tites in passive skin anaphylaxis and by the results of the basophil degranulation test, than the appearance of delayed hypersensitivity detected by the leukocyte migration inhibition test.

Allergens↗
PubMed1975

The direct basophil degranulation test and the intracutaneous test: a comparison using food extracts.

The purpose of this study was to compare the direct basophil degranulation test (DBDT) of Hirsch and Zastrow with the intracutaneous (IC) test in subjects with and without histories of food allergy. The DBDT was perfomred by incubating the concentrated leukocytes of subjects with dilutions of the suspected allergenic extract and comparing the number of nondegranulated basophils remaining in the test samples with the number remaining in a control. The test sample with the least number of basophils represents maximal basophil degranulation. The DBDT was perfromed with extracts of the suspected food on 14 subjects with reliable clinical histories and positive IC tests indicative of food hypersensitivity. Thirteen of these 14 food-sensitive subjects also had IC and DDBT8 to the extracts of foods to which they were not clinically sensitive. Fourteen different subjects not allergic to foods were also tested with food extracts by the IC and DBDTS. There was a 92% correlation between the IC test and the DBDT, a 90% correlation between the IC test and the history, and an 86% correlation between the DBDT and the history. We conclude that, in this study of subjects clearly sensitive to foods, the DBDT correlates favorably with the history and IC test.

Allergens↗
PubMed1981

[Interpretation of results on a human basophil degranulation test (author's transl)].

A statistical analysis of a result on a human basophile degranulation test was performed. The shape of the curve representing the number of basophiles, as a function of the logarithm of the allergenic dosage, was parabolic, in the case of degranulation. This curve permits a calculation of the dosage necessary to provoke a degranulation of 10 per cent, in comparison with the mean of the two control test tubes. The authors also determined the significant degranulation threshold to be 5 per cent, as regards the mean number of cells counted for the controls (example: for 50 control cells counted, the threshold was equal to 28 per cent degranulation). This statistical analysis largely facilitates the interpretation of the granulation test by basophiles, by assuring the value of observed degranulations, and by allowing a better quantification of the phenomenon. The latter might permit a comparison of different batches of allergen, that is, the standardisation of allergens. These calculations can be easily programmed, using a currently available calculator.

Basophils↗
PubMedDate not supplied

[Human basophil degranulation test in the diagnosis of anguilluliasis. Preliminary results].

The human basophil degranulation test was applied to the diagnosis of strongyloidiasis. The preliminary results show an excellent response. 33 of the 35 cases give a definite positive result and in the 15 control cases, with no parasitosis or an other helminthiasis, the test was negative. If these results are confirmed by a more important study of sensitivity and specificity, the test would be of interest when the parasitosis is of difficult evidence and especially by patients needing immunodepressive therapy.

Animals↗
PubMed1983

[Human basophil degranulation test and double diffusion in pulmonary hydatidosis].

A slide human basophil degranulation test (HBDT) and a double-diffusion test were done in 37 patients with suspected pulmonary hydatid disease. Among the 31 patients in whom a diagnosis was firmly established, 22 had a pulmonary hydatid cyst and 9 did not (controls). The HBDT was at least 35% in 17 of the 22 patients with hydatid disease; 16 of these exhibited a precipitation arc. Among the 9 controls, none had a HBDT above 30% and three had a precipitation arc. In this localization of hydatid disease, compared to the double-diffusion technique, the HBDT has similar sensitivity (0.77 versus 0.73 but higher specificity (1 versus 0.67).

Basophils↗
PubMed1986

[Human basophil degranulation test in the diagnosis and prevention of peranesthetic anaphylactoid complications].

The human basophil degranulation test (HBDT) studies the allergic response of effector cells. In this work, it was used in the diagnosis of anaphylactoid reactions during anaesthesia. Thirteen patients having had an anaphylactoid shock during general anaesthesia were investigated. An HBDT was performed with every suspected drug used during the anaesthesia. Ten patients had a positive HBDT and in five cases the drug involved was suxamethonium. In one case, the IgE-dependency of the accident was asserted by the ability of the plasma to sensitize in vitro alien basophils. These sensitized basophils degranulated in the presence of Althesin and suxamethonium. Passive sensitization was abolished when using plasma heated for 2 h at 56 degrees C. Two patients had a negative HBDT to 11 drugs tested. To assess the validity of these results, and to rule out unspecific degranulation, thirteen control patients who were to undergo general anaesthesia were investigated. HBDT was performed immediately before anaesthesia in a double-blind method with every drug to be used during the anaesthesia. The twelve patients who had a negative HBDT to every drug had an uneventful anaesthesia. One patient had a positive test to thiopentone and displayed bronchospasm and generalized urticaria during the anaesthesia. In conclusion, the HBDT appears to be a reliable test in the diagnosis of anaphylactoid reactions during anaesthesia.

Anaphylaxis↗
PubMed1980

Modified basophil degranulation test in diagnosis of bee and wasp sting allergies.

A modified basophil degranulation test proved reliable and easily reproducible in the investigation of 50 patients with allergies to bee and/or wasp stings. Its diagnostic value is comparable to that of the RAST and it is, in addition, simpler, quicker and cheaper. Its disadvantage is that it has to be performed within 24-36 hours after withdrawal of blood.

Adolescent↗
PubMed1979

[The basophil degranulation test in the diagnosis of bee and wasp sting allergies].

Comparison of basophil degranulation test (BDT) with radioallergosorbent test (RAST) in 50 patients with anamnestically certain or probable hymenoptera allergy yielded practically equal results. In patients who had experienced their allergic reaction a long time ago, BDT seemed to react positively over a longer period of time than RAST. As BDT is technically easy to perform and moderate in price, it is suited to substitute RAST.

Adolescent↗
PubMed1987

[Value of the basophil degranulation test (BDT) in the follow-up of immunotherapy with hymenoptera venoms].

The basophil degranulation test (BDT) with the plasma of 24 patients undergoing venom immunotherapy was usually negative after only 6 months of treatment. Cellular sensitivity, however, could still be proved by BDT on purified cells, i.e. after removal of the plasma which contained blocking IgG antibodies. In 30% of the cases, we found primary desensitization of the basophils, which means that the IgG antibodies did not have any influence on the test results.

Basophils↗
PubMedDate not supplied

Basophil degranulation test in house dust mite allergy: diagnostic value.

After reviewing the experience of various authors we applied the Basophil Degranulation Test (BDT) in the study of sensitization to Dermatophagoides pteronyssinus, so as to evaluate its diagnostic use. Fifty-five patients with asthma and/or rhinitis and a positive skin test to the mentioned antigen, as well as 20 control subjects were studied. The results demonstrated a sensitivity of 65.5% and a specificity of 80% with an efficiency index of 69.5%. We did not find any significant correlation with the histamine release test (r = 0.236). The data obtained and the proper limitations of the technique led us to consider the BDT to have a limited application in routine allergy diagnosis.

Adolescent↗