Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “BRUCELLOSIS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 19 recordsLinked to original sources
PubMed1988

Assessment of Brucellosis Card test in screening patients for brucellosis.

The Brucellosis Card test (Brewers' Diagnostic Kits, Hynson, Westcott and Dunning, Inc., Baltimore, Md.) was evaluated in relation to the Brucelloslide test (bioMérieux, France), the microagglutination test (MAT) and the demonstration of brucella-specific IgG, IgM and IgA in an enzyme-linked immunosorbent assay (ELISA). A total of 573 serum specimens was tested. These included sera from patients with acute brucellosis (159), chronic brucellosis (23) and patients who had been diagnosed previously as having had brucella infection (155). Control groups consisted of patients with diseases other than brucellosis (52), others with non-infectious diseases (20), and healthy individuals (164). The Card test detected 100% of the patients with acute and 61% of the patients with chronic brucellosis. The sera from the control groups were all negative. Similar results were obtained with the Brucelloslide test and the MAT. The ELISA test detected brucella-specific Ig of all classes in the serum of patients with acute brucellosis, and IgG and IgA in the serum of patients with chronic brucellosis. In the latter group, IgM was also detected in 32% of the sera. Twenty-three per cent of sera with titres of 20 by the MAT were positive on the Card test and had ELISA titres for IgM, IgG and IgA of 400. Characterization of the antibodies involved in the Card test showed that sera with IgM ELISA titres of 1600, or an IgM titres of 800 together with IgG and IgA titres greater than or equal to 200 were Card test positive. Higher IgG (greater than or equal to 1600] plus IgA (greater than or equal to 400) titres were required to produce a positive Card test in the absence of IgM or when the IgM titre was less than or equal to 200. The Card test has a potential value as a rapid screening test for humans with acute brucellosis and shows similar results to Brucelloslide and MAT tests. ELISA, however, remains the most reliable test for diagnosis of brucellosis especially in patients with chronic and complicated stages of the disease.

Acute Disease↗
PubMed2002

How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing.

Collaborative work was financed by the EU to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to Yersinia enterocolitica O:9 either in conjunction with, or as an alternative to, the classical serological, bacteriological or allergic skin tests. Sixteen heifers were experimentally infected with Brucella abortus biovar 1 (five heifers), Brucella suis biovar 2 (two heifers), Y. enterocolitica O:9 (six heifers) and Y. enterocolitica O:3 (three heifers). Four heifers, naturally infected with Y. enterocolitica O:9 that presented aspecific brucellosis serological reactions were also included in the experiment. A self-limited infection was induced in cattle by B. suis biovar 2. All the brucellosis serological tests used, i.e. the slow agglutination test (SAW), the Rose Bengal test (RB), the complement fixation test (CFT), indirect and competitive ELISA's, lacked specificity when used to analyze sera from Y. enterocolitica O:9 infected animals. A Yersinia outer membrane proteins (YOPs)-ELISA was also used and although the test is able to detect a Yersinia group infection, it provided no evidence of whether or not there is a possible brucellosis infection when dual infections are present. The brucellergen IFN-gamma test showed a lack of specificity also. The only test that was proven to be specific is the brucellergen skin test. All brucellosis serological tests, except the indirect ELISA, were limited in their ability to detect B. abortus persistently infected animals. Based on these experimental studies, a strategy was implemented as part of the year 2001 Belgian Brucellosis Eradication Program to substantiate the eradication of bovine brucellosis. Epidemiological inquiries have identified risk factors associated with aspecific serological reactions, possible transmission and infection of cattle by B. suis biovar 2 from infected wild boars; and both legal and administrative measures taken by the veterinary services. No cases of bovine brucellosis have been confirmed in Belgium since March 2000.

Animals↗
PubMed1991

[Activity of adenosine deaminase in acute brucellosis and complicated brucellosis].

BACKGROUND: Adenosine deaminase (ADA) is an essential enzyme for the differentiation and proliferation of T lymphocytes and the monocyte-macrophage system. The basic immunitary response of brucellosis is cellular. To this end, ADA activity was evaluated in brucellosis. METHODS: Serum ADA activity was assessed by a colorimetric method in 67 patients with brucellosis, before therapy and at 1, 3 and 6 months of follow up. RESULTS: Serum ADA activity in brucellosis was higher than that in 52 healthy controls, both in those with the acute febrile noncomplicated form (48 patients) and in those with focal symptoms from one organ (19 patients) (p less than 0.0001 and p less than 0.001). There were no differences between both groups of brucellosis. There was a negative correlation between the duration of the disease and ADA activity. After therapy there was a rapid decrease of ADA values, more marked in patients with noncomplicated brucellosis. During the follow up, only one patient had a new increase in ADA activity, coincident with a clinical and bacteriological relapse, and previous to the increase of IgG anti-Brucella titers. CONCLUSIONS: The results indicate that adenosine deaminase activity is increased during the active stage of brucellosis. It can be considered as a biochemical follow up marker of the disease and, probably, as a marker of relapses.

Acute Disease↗
PubMed1994

Tolerance of the human brucellosis vaccine and the intradermal reaction test for brucellosis.

The aim of the present study was to determine the tolerance and efficacy of human brucellosis vaccine as well as the tolerance and sensitivity of the intradermal reaction test for brucellosis. A total of 1,121 subjects initially consented to participate in the study. Following clinical examination, 762 were tested with the intradermal reaction test (IDR Test, Pasteur Merieux, France), of whom 576 with no previous or current brucellosis infection were accepted for vaccination. Two hundred seventy-one were vaccinated with brucellosis vaccine and 305 with tetanus vaccine, the latter group serving as a comparative control group. The vaccinated persons were monitored monthly for at least nine months. Although brucellosis vaccine caused some unpleasant side effects in about one-fourth of the vaccinated persons, it appears safe for large-scale use in prevention programs. In addition, the results indicate that the intradermal reaction test is safe and sensitive for large-scale use in prevention programs. The test was positive in 28 of 30 individuals with previous infection, representing a sensitivity of 93.33%.

Adolescent↗
PubMed1981

[Genetic problems of cattle resistance to brucellosis. I. The age of having the disease, the paternal influence and strain affiliation in the incidence of brucellosis morbidity].

From 2810 animals of black-and-white breed of Novosibirsk region diagnosed, 31% fell ill. Young animals were found to be more resistant to brucellosis. It is evidently more probable that they can reach the reproductive age. Intrapopulation hereditary heterogeneity to brucellosis was found. The progeny of certain bulls has a higher resistance than others. There is no statistical reliability of differences in the frequency of the disease between animal lines. A normal distribution of fathers according to the frequency of daughters diseases can point to a polymeric type of the inheritance of resistance to brucellosis. The coefficient of inheritability of brucellosis is 0,194 +/- 0,03. The purebredness and mongrel of animals did not influence the frequency of the disease. The crossing of cows of black-and-white breed with bulls of Holland black-and-white breed did not influence the frequency of the disease of the hybrids of the first generation.

Aging↗
PubMedDate not supplied

[Professional brucellosis and endemic brucellosis in a given area].

An epidemiological survey was carried out in an area where there is an abattoir/canning factory in which a recrudescence of cases of professional brucellosis was observed: whilst there had been 29 cases of brucellosis in 8 years, 10 cases were confirmed in 1970. Our survey had underlined a brucellosis endemy in the population of that area: 32.50% of the patients had agglutinating or complement-fixing antibodies (698 people were examined). Results obtained have brought us to discuss the professional origin of brucellosis.

Abattoirs↗
PubMed1980

Brucellosis in moose (Alces alces). A serological survey in an open range cattle area of North Central British Columbia recently infected with bovine brucellosis.

A serological survey for Brucella abortus antibodies in mature cow moose (Alces alces) was made in an area of northcentral British Columbia which recently had been heavily infected with bovine brucellosis and in which there was considerable intermixing of moose and range cattle. No evidence of Brucella infection was found in the moose tested and it was concluded that they were probably not of great significance in the epidemiology of bovine brucellosis in the study area and were therefore unlikely to have hindered attempts to eradicate brucellosis from the cattle in that area.

Animals↗
PubMed1975

[Diagnosis of human active or latent human brucellosis by lymphocyte transformation. Inhibition of leukocyte migration and passive hemagglutination. Possible interference between brucellosis and some mental disorders].

Passive hemagglutination tests (H.T.), involving the coating of a soluble B. abortus antigen onto sheep red blood cells through chromium chloride, were always negative in non-brucellic subjects. Positive H.T., even at low titers (1:50), were associated with positive specific lymphoblastic (T.T.L.) and inhibition of leucocyte migration (I.M.L.) tests in 13 patients. These 3 tests were negative in 17 control brucellosis-free individuals. Positive H.T. confirmed a clinical diagnosis of brucellosis in 27 patients with dubious or negative responses to classical tests. Low positive (1:50, 1:100) H.T. may correspond to Brucella primary-infection or to a quiescent chronic infection, and higher titers to clinically active brucellosis. There is a correlation between cellular immunity tests (T.T.L., I.M.L.) and passive hemagglutination test. Furthermore, 39/88 women hospitalized in psychiatric wards were positive to H.T., and 6 of them were also highly positive to complement fixation and tube agglutination tests. Three T.T.L. and I.M.L., performed on samples from 3 H.T. (1:50) positive patients, were positive. These 30 patients were classified as depression or severe anorexia.

Adult↗
PubMed1982

[Importance of cross reactions in evaluating the serological diagnosis of human brucellosis. II. Examination of brucellosis patients by the passive hemagglutination reaction using homologous and heterologous erythrocyte diagnostica].

The study of 270 serum samples obtained from chronic brucellosis patients in the passive hemagglutination test have revealed that in the presence of specific brucellosis hemagglutinins cross reactions with Yersinia antigen may occur. This phenomenon is probably due to the presence of common antigenic determinants in the lipopolysaccharide fractions of Brucella abortus 99 and Yersinia enterocollitica 09. The passive hemagglutination test has revealed that 2-mercaptoethanol-sensitive antibodies (IgM) to both homologous and heterologous antigens are mainly present in chronic brucellosis patients.

Antibodies, Bacterial↗
PubMed1993

Model for assessing the risk of introducing brucellosis into a brucellosis-free area.

A risk assessment model is presented, for use by local decision-makers to aid the evaluation of proposed changes in existing brucellosis eradication or control programmes. This model provides a format and structure for gathering and analysing data. The model uses data which are generally available and accessible, so that minimum time, expense and effort are required for collection. The use of this model enables an estimation of the risk of introduction of brucellosis into a non-infected population, based on the probability of importing the agent and subsequent spread, given the existence of specified surveillance and control measures. The model creates a point estimate of the risk associated with a given set of conditions.

Animals↗
PubMed1982

[Importance of cross reactions in evaluating the serological diagnosis of brucellosis in human beings. 1. The results of the examination of chronic brucellosis patients using the agglutination and Coombs' tests with Brucella and Yersinia (serotype 0-9) antigens].

The study of blood serum samples obtained from 187 patients with chronic brucellosis revealed that in those cases when blood serum contained specific antibrucella antibodies cross reactions with Y. enterocolitica 0-9 antigen occurred in the agglutination test and Coombs' test. The results thus obtained showed that Y. enterocolitica 0-9 antigen could be used for detecting mainly 2-mercaptoethanol-sensitive complete and incomplete antibodies (IgM). This study indicates that serological cross reactions play an important role in the evaluation of the specific methods for diagnosing brucellosis.

Agglutination Tests↗
PubMed1982

The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests.

Serum sample were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests. The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions. In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera. Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

Agglutination Tests↗
PubMed1995

Bovine brucellosis in the Highveld veterinary region: a preliminary report on the seroprevalence of brucellosis amongst adult beef cows in the region.

A preliminary survey to determine the seroprevalence of bovine brucellosis amongst adult beef cows in the Highveld veterinary region was conducted. Based on complement fixation tests, about 48.2% of seropositive beef herds and 4.47% of seropositive individual animals could be expected in the region. Since heifer calves on some farms apparently receive their doses of strain 19 vaccine later than the prescribed age of 4 to 8 months, more vaccination reactions than would normally occur, could be expected. The above figures should therefore be considered an over-estimation.

Animals↗