[Changes in total blood proteins, electrophoresis, hematocrit & blood oxymetry in relation to bloodletting performed on normal subjects].
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A higher survival rate and faster wound healing in dogs on a controlled and well-balanced diet have been demonstrated when compared with those not under dietetic control.Electrophoretic comparison of serum proteins was carried out in two groups of dogs. The first was on a well-controlled and well-balanced diet; the second group received an uncontrolled diet. The dogs in Group 2 had a lower concentration of total proteins, and globulins, especially gamma-globulins, were diminished; however, the serum albumin was higher. The albumin/globulin ratio was 0.93 in Group 1 and 2.07 in Group 2.Serum electrolyte levels also showed some slight differences. Sodium and potassium levels were higher in well-nourished dogs, while chloride and bicarbonates were slightly lower.
Genetic variation of 31 blood protein loci in 236 cattle from eight South China populations (including mithan, Bos frontalis) and a Holstein population was investigated by means of horizontal starch gel electrophoresis. Thirteen loci (ALB, CAR, Hb-b, Np, PGM, Amy-I, PEP-B, AKP, 6PGD, Cp, Pa, EsD, and TF) were found to be polymorphic. The comparison of average heterozygosities (H) shows that all the native cattle embrace a rich genetic diversity. Our results on protein polymorphism suggest that cattle in China originated mainly from Bos indicus and Bos taurus; Xuwen, Hainan, Wenshan, and Dehong cattle and the Dehong zebu are close to zebu-type cattle, and Diqing and Zhaotong cattle are close to the taurine. The mithan was very different from other native cattle, and we suggest that its origin was complicated and may be influenced by other cattle species.
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In 6 Chinese yak (Bos. grunniens) populations including 177 yaks, 34 blood protein loci were studied by horizontal starch gel electrophoresis, four of these loci (AKP, ALB, LDH-1, TF) were found to be polymorphic. The percentage of polymorphic loci(P) is 0.118, the mean individual heterozygosity(H) is 0.015, which means a low level of genetic diversity in the whole Chinese yak population. The coefficient of gene differentiation (Gst) is 0.0625, which indicated an almost-indistinguishable divergence among different populations at the level of blood protein electrophoresis.
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Protein electrophoresis was used to examine the blood protein polymorphism in Yunnan local pig breeds, i.e., the Saba pig, Dahe pig, and Diannan small-ear pig breeds. Of 38 genetic loci surveyed, 9 were found to be polymorphic. The percentage of polymorphic loci (P) varies from 0.1875 to 0.2121, and the mean individual heterozygosity (H) varies from 0.0712 to 0.1027 in three pig breeds. The results indicate that blood protein polymorphism in Yunnan pig breeds is high. Yunnan local pig breeds have a wealth of genetic diversity at the level of blood proteins.