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PubMedDate not supplied

Dihydroxyacetone, pyruvate, and phosphate effects on 2,3 DPG and ATP in citrate-phosphate-dextrose-adenine blood preservation.

Blood was stored with various combinations of 60 mM dihydroxyacetone (DHA), 80 mM pyruvate and 10 mM phosphate in citrate-phosphate-dextrose-adenine-one (CPDA-1) preservative for 42 days. During that time, DHA was found to have a beneficial effect on 2,3 diphosphoglycerate (2,3 DPG), although a deleterious effect on adenosine triphosphate (ATP). Preservatives containing pyruvate gave similar results. However, DHA and phosphate together in CPDA-1 improved both 2,3 DPG and ATP. The adverse effect on 2,3 DPG levels that phosphate normally shows was reversed by the addition of DHA. These results indicate that the addition of phosphate alone, rather than pyruvate, or pyruvate and phosphate, to DHA in CPDA-1 would be preferable to the presently licensed CPDA-1 preservative.

2,3-Diphosphoglycerate↗
PubMed1994

[Personnel need for preoperative establishment of autologous blood preservation compared with allogenic blood preservation].

Based on the CAP-Laboratory Workload Recording Method, we compare staffing needs for our preoperative autogenous blood deposit program (PAB) with those for routine allogeneic blood donation (ABD) in our hospital-based community blood center. For the preparation of 1,000 ABD-units, we need 0.17 clerical, 0.34 nursing, 0.47 technologist and 0.27 medical staff full time equivalents (FTE) compared to 0.17/0.71/1.23 and 0.36 FTE, respectively, for 1,000 PAB containing one unit from each donor-patient. For 1,000 PAB containing 4 units from each donor-patient, we require 0.68/2.84/3.96 and 1.38 clerical/nursing/technologist and medical staff, respectively.

Blood Banks↗
PubMed1990

Reticulocytes in human preserved blood as control material for automated reticulocyte counters.

The authors studied the changes in the percentage of reticulocytes in blood preserved for three weeks. The samples were obtained from healthy adults and stored at 4 degrees C. An automated reticulocyte counter was used for measurements. After one week, the relative percentage of reticulocytes had decreased to around 80% of the initial value, and it reached 60% after three weeks of storage. When the y-axis of plots of the change against time was converted to a log scale, negative correlation was strong (r = -0.9972), and the curve was useful for estimation of the reticulocyte count in preserved blood. Stored blood might be used as a control material for reticulocyte counting by automated methods.

Adult↗
PubMed1987

[Preservation of the blood of slaughter animals by the non-diaphragmatic use of solidified carbon dioxide. III. Effect of blood preservation. Practical blood stability].

The preservation effectivity of slaughter animal blood for consumptive purposes was studied by a method of non-phrenic action with solidified CO2. The material for studies was full pig blood stabilized with sodium citrate. The course of the changes of the blood properties studied has been described by regression equations. Blood stabilization with solidified CO2 was found to reduce the rate of aerobic microflora growth and of proteolytic changes and to extend the stability period of the coagulation system. The results of sensory studies were related to the boundary values of the studied physicochemical and microbiological quality factors. The admissible periods of blood storage were determined from the results of the studies. It was found that practical preservation of blood stabilized with solidified CO2 was 15 full days at 5 degrees C, 6 days at 10 degrees C and 4 days at 15 degrees C, and it was two, three and four times greater at the three successive temperatures, as compared with the preservation of control blood.

Animals↗