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At least 19 recordsLinked to original sources
PubMed1982

[Importance of the bacteriologic study in brain abscess. Comparison of the evolution of bacteriologic and therapeutic results in a series of 102 cases].

Progresses in bacteriological study have been analyzed on 102 cases of intracranial infections between 1968 and 1980: sterile cultures decrease from 56% to 11,5%, rate of isolation of anaerobic and association of aero-anaerobic bacteria increase respectively from 10 to 40% and from 2 to 17,1%. Clinical results progress in close relation; the mortality rate decrease from 11,5% to 8,3% as well as morbidity rate from 33% to 19,4%. Moreover clinical results are worse when the bacteriological study fails to isolate any germ: mortality 15,7% and sequellae 53,5%. The best results are observed in the group of anaerobic infections: mortality 4% and sequellae 26,6%. Lastly, the interest of the systematic research of particular bacteria such as capnophilic or microaerophilic bacteria or mycobacterium tuberculosis (2 cases) is underlined. This work emphasizes the importance of the bacteriological study for the management of intracranial infections and the improvement of the clinical results.

Bacteriological Techniques↗
PubMed1992

[Bacteriology of infectious disease in otorhinolaryngology (1). Bacteriological study of paranasal cyst].

The bacteriology of chronic sinusitis has been reported in several studies, but there have been few bacteriological studies on paranasal cyst. Also, details of the techniques of transportation and processing of clinical specimens have only rarely been described. Therefore, this study was undertaken with attention to the above, and to obtain information on appropriate antimicrobial therapy, we conducted a dilution antimicrobial susceptibility test of isolates. Fifteen patients with paranasal cyst participated in this study. Their lesions were frontal sinus (3), ethmoid sinus (2), sphenoid sinus (2) and maxillary sinus (8). We punctured the cyst using an 18-gauge needle attached to a syringe at the time of operation, and paranasal cyst effusions were promptly transported to the laboratory. The specimen was immediately inoculated under a set of aerobic and prereduced anaerobic plates, which were incubated in appropriate conditions. Most specimens were completely processed within one hour. Antimicrobial susceptibility testing of isolates was done with MIC panel by the Sceptor system. Positive results of bacterial cultures were found in 60% of all cases (all of the frontal sinus specimens and half of those with other lesions). A total of 38 bacteria, including 24 anaerobic and 14 aerobic species, were isolated from 9 paranasal cysts, yielding an average of 4.2 species per positive case (2.9 anaerobes and 1.3 aerobes). Monomicrobial flora were found, anaerobes only in 2 cases and aerobes only in 2 others. Mixed flora were found in 5 cases in which both anaerobes and aerobes were isolated. The bacterial concentration in the maximal case was 4.9 x 10(4) CFU/ml. The organisms most frequently isolated were Propionibacterium spp. (7), Peptostreptococcus spp. (7), Staphylococcus spp. (7), Prevotella spp. (5) and Streptococcus spp. (4). On the basis of the results of antimicrobial susceptibility testing of isolates in vitro, the use of Cephems, Clavulanic acid/Amoxicillin, Norfloxacin and Chloramphenicol proved to be clinically effective.

Adult↗
PubMed1993

Bacteriology of tonsil and adenoid and sampling techniques of adenoidal bacteriology.

The value of pernasal swabs and direct adenoid swabs in chronic adenoid and adenotonsillar disease was assessed in 175 patients. Prior to adenoidectomy (53 patients) or adenotonsillectomy (122 patients), pernasal and direct adenoid swabs were taken. Adenoid currettings and tonsil tissue were cultured. Haemophilus influenzae was the bacterium most frequently isolated from adenoid currettings and from the centre (core) of the resected tonsil. There was a close relationship between the bacteriology of the pernasal swab and the adenoid tissue and tonsil core in 72 and 71% of patients, respectively. There was an identical profile of pathogens in 52 and 49%, respectively. We suggest that in children with adenoiditis or adenotonsillitis and hypertrophy of the adenoid, a pernasal swab should be used in preference to a throat swab in selecting appropriate antimicrobial therapy. Penicillin and ampicillin are not appropriate blind therapy in chronic adenoid and adenotonsillar infections because of the prevalence of beta-lactamase-producing aerobes (40%) in adenoid and tonsil core in these conditions.

Adenoidectomy↗
PubMed2001

[Soybean bacteriological culture media and prospects for their use in clinical bacteriology].

Agar bacteriological nutrient media are suggested, based on soybean extract subjected to enzymatic hydrolysis and lyophilized native. The growth of test strains, number of grown colonies and their size were virtually the same as after inoculation of these strains in control nutrient media. Soybean salt medium with yolk suspension was tried with good results as elective medium for isolation of staphylococci from clinical material. Commercial manufacture of these media mainly from native soybean extract is proposed.

Bacteriological Techniques↗
PubMed1978

Bacteriological contamination of dialyzers. Clinical, bacteriological and scanning electron-microscopic evaluation of different dialysate mixing systems.

Membranes from Gambro Lundia Nova dialyzers were investigated for presence of bacteria after hemodialysis with centrally and peripherally mixed dialysate. Centrally mixed dialysate was found contaminated with Pseudomonas aeruginosa, and bacterial cultures as well as electron scanning micrographs showed the presence of these bacteria on the dialysate side as well as on the blood side of membranes from dialyzers perfused with this dialysate during hemodialysis. No bacteria were found on membranes used with peripherally mixed dialysate, in which very few bacteria were found. Despite this the frequency of febrile episodes in patients submitted to hemodialysis with the two different dialysate mixing systems showed no significant difference in our material.

Bacteria↗
PubMed1981

The bacteriology of acute otitis media in children with special reference to Streptococcus pneumoniae as studied by bacteriological and antigen detection methods.

The middle ear fluid (MEF) was studied during an acute attack of otitis media in 519 children, aged 3 months to 6 years. Streptococcus pneumoniae (Pn) was cultured from 39% of the cases; serotypes 19, 6, 3 and 23 were the most common. Haemophilus influenzae (Hi) was cultured in 12%; only 2/64 strains were of type b. Pn were found equally often in all age groups, but Hi were significantly less often isolated in children older than 3 years. The number of negative cultures increased with the age of the child. Pneumococcal capsular polysaccharide was detected with counterimmunoelectrophoresis and/or latex agglutination in 83% of the MEFs from which Pn were cultured, but also in about one third of the MEFs from which no bacteria could be grown. Altogether, with these methods combined Pn were implicated in nearly 60% of the cases of acute otitis media. Gram staining showed polymorphonuclear leucocytes in 85% of pneumococcal otitis cases that were verified by culture but also in 72% of the cases from which no bacteria could be cultured, supporting the contention that also these latter are usually caused by bacteria.

Acute Disease↗