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The benefits of animal identification for food safety.

The Center for Veterinary Medicine supports the effort to have a practical, workable form of mandatory animal identification. An animal identification system will make tracing of the source of animals with drug or chemical residues quicker and more effective. One of the best means of addressing and solving the problem of residues is through mandatory livestock identification. A successful traceback benefits both the producer and the industry. The U.S. Department of Agriculture (USDA) has proposed a mandatory nationwide system to require that all swine in interstate commerce be identified. Under the proposal several means of identification are listed and could be used. Additional methods or devices could be requested in writing to USDA. Persons required to keep records under the system would maintain the documents at their place of business for 2 yr. Records would be available to authorized USDA employees during ordinary business hours. FDA has had difficulty tracing tissue residue violations, particularly those involving sulfamethazine residues in swine. Investigations involving culled dairy cows and veal calves also have been closed due to lack of producer identification. The ability for FDA to determine the source of residues is vitally important in a coordinated government program to eliminate illegal tissue residues.

Animal Identification Systems

Microchip implant system used for animal identification in laboratory rabbits, guineapigs, woodchucks and in amphibians.

Traditional methods for animal identification have a number of drawbacks. We evaluated a new system for individual identification using microchip implants in rabbits, guineapigs, woodchucks (Marmota monax) and amphibians (Xenopus laevis, Pleurodeles waltlii). Implantation procedure and long-term observations are described. Microchip implants proved to be a practicable and reliable system for animal identification without obvious adverse effects. The applicability of electronic animal identification in comparison with common methods and with regard to animal welfare and legal aspects is discussed.

Amphibians

A visual display board used for efficient breeding and utilization of an athymic (nude) mouse colony.

A visual display board was designed to aid in the management of a barrier sustained athymic (nude) mouse colony. The board displayed pertinent information for breeding and weaning, including phenotype and age for each animal in the colony. In addition, the board showed the availability and current status of experimental groups. This system provided an efficient means of organizing production and planning utilization of animals in the colony.

Animal Identification Systems

Computer record keeping in a large rodent colony.

An automated information system was developed to aid in the record keeping of inbred rodent breeding colonies. The system had keyboard terminals in each breeding room linked to a local mini-computer for data collection, editing, and short-term storage; and a remote, large computer center for permanent storage and report generation. The system allowed an animal technician to record all the activities associated with an inbred rodent breeding colony such as recording births of litters, weanings, assignment of animals, disposition of animals, and health observations. The information collected was available in daily reports provided within 24 hours after observations were recorded; weekly compilations that summarized the daily events; and special reports such as pedigrees, which were available on request.

Animal Identification Systems

Accuracy of microchip identification in dogs and cats.

A study was performed to determine the sensitivity and specificity of a commercially available microchip identification system approximately 1 year after implantation in dogs and cats. Thirty-three dogs and 16 cats in which a microchip had been implanted and 31 dogs and 18 cats in which a microchip had never been implanted were included in the study. In cats, sensitivity and specificity of microchip identification were 1.00. In dogs, sensitivity and specificity were 0.97 and 1.00, respectively. The chip had migrated in the 1 dog with a false-negative result, but the chip remained functional, and identification was established in this dog following closer physical examination and scanning.

Animal Identification Systems

[Effect of marking laboratory animals on the results of pertussis vaccine potency tests].

White mice used for protection tests with pertussis vaccines when marked with fuchsin before challenge showed reduced survival rates compared with their equally treated but unmarked cage mates. The fuchsin used had been dissolved in denaturated alcohol. On account of the small amounts of marking material applied, there was probably no direct toxic effect. Further methods of marking have then been studied in this delicate test system. Contrary to expectations the marking with picric acid solution showed no adverse effect at all. The shearing of an area of the fur, however, resulted in an even more detrimental effect than the use of fuchsin. The clipping of the top of one ear rested below the level of significance. Most probably the marked mice were neither affected adversely by the handling when marked nor by an actual chemical or mechanical impairment, but by the initiation of stress induced by the majority of their unmarked cage mates. At any rate, a possible influence of the marking of animals on the result of test procedures should be carefully considered.

Animal Identification Systems

Mn concentration in organs of rats after implantation of battery cathode material.

The distribution of Mn, originating from MnO2 in miniaturized batteries, was studied in rats. Forty young male Wistar rats were included in a randomized block experiment with body weight, weight gain, relative organ weight, and the concentration of Mn as dependent variables. Mn containing cathode material was implanted subcutaneously and after 1 month, animals were sacrificed and organs collected. Mn levels were determined by ICPES after destruction of the organic matter using microwave energy. Body weight, weight gain, and relative weight of heart, brain, liver, and kidneys were not significantly altered after implantation of cathode material. However, Mn levels were largely increased in the four organs under study. These high levels did not provoke any of the well-known clinical signs associated with Mn intoxication. Effects of Mn in larger farm animals can be considered as insignificant.

Animal Identification Systems

Occlusive patch method for skin sensitization in guinea pigs: the Buehler method.

Currently, the European Community is in the process of discussing the details of how to conduct various protocols for safety assessment. Additionally, there is a desire that these protocols be harmonized internationally. This presentation attempts to describe the critical and non-critical parameters of the protocol for detecting potential contact allergens in the guinea pig and also discusses some controversial issues. It is also emphasized that the identification of a test material as a potential sensitizer is only the first step in the risk-assessment exercise.

Animal Identification Systems