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A numerical taxonomic study of members of the Actinomycetaceae and related taxa.

Two hundred and twenty-two representatives of the Actinomycetaceae and related taxa, including reference cultures, received strains and fresh isolates, were tested using 124 unit characters. The data were examined using numerical taxonomic techniques with various coefficients and average linkage clustering; the variation in composition of the clusters obtained from the different coefficients was slight. Most species included in the study formed discrete phena, which exhibited good differential characters. Actinomyces israelii was particularly well-defined and A. naeslundii and A. viscosus, although grouping together, did show divergence in several test results. Actinomyces bovis was initially linked with representatives of genera other than Actinomyces, these being Bifidobacterium, Corynebacterium pyogenes and Erysipelothrix rhusiopathiae. The strains of Bacterionema matruchotii and Rothia dentocariosa formed tight distinct clusters associated only at low similarity levels with other members of the family Actinomycetaceae. The Arachnia propionica phenon, although well-defined, appeared to consist of two subclusters which could be assigned to serotypes 1 and 2. Tests which may be useful in identification are tabulated and the relationships between the various taxa are discussed.

Actinomycetaceae↗

Rapid identification of Actinomycetaceae and related bacteria.

Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.

Actinomycetaceae↗

Taxonomy and clinical significance of Actinomycetaceae and Propionibacteriaceae.

In spite of considerable advances during the last years, classification and identification of Actinomycetaceae and Propionibacteriaceae still present major problems. Uncertainties about the systematic position and taxonomic status of several genera and species, the lack of reliable distinguishing characteristics or use of inadequately adapted techniques of investigation interfere with both micribiological and clinical research and also with routine work in the diagnostic laboratory. Nevertheless, besides actinomycosis, an etiologically well defined suppurative infection, several other diseases and lesions such as lacrimal canaliculitis, caries and periodontal disease, inflammation following use of intrauterine pessaries, various types of abscess, septicaemia, and acne vulgaris have been attributed to the direct or indirect pathogenic effects of fermentative actinomycetes, propionibacteria, or eubacteria. Many questions concerning their individual pathogenicity and their pathomechanisms still remain to be answered, however.

Acne Vulgaris↗

Expression of trypsin-like activity by the genera Corynebacterium and Actinomyces in canine periodontitis.

Trypsin-like activity (TLA), clinical parameters and TLA-positive bacteria were examined in periodontitis and healthy sites in dogs. TLA was markedly higher in periodontitis than at healthy sites. There was good correlation between TLA positivity and severity of periodontal disease. The proportions of TLA-positive bacteria to total isolates in periodontitis and healthy sites were 21.1% and 2.1%, respectively. Among TLA-positive bacteria in periodontitis sites, 4.4% showed strong TLA activity, 35.3% showed moderate and 60.3% showed weak activity. In the healthy sites, all the TLA-positive bacteria showed weak activity. In all, 90% of the total number of TLA-positive bacteria were identified as belonging to the family Actinomycetaceae; 40% of bacteria belonging to the family Actinomycetaceae were identified as genus Corynebacterium with moderate trypsin-like activity and the remaining 60% were identified as genus Actinomyces with weak activity. Obligately anaerobic bacteria accounted for only 5.9% of the total population of TLA-positive bacteria; they were gram-negative coccobacilli, gram-positive rods and gram-positive cocci. These observations suggested that bacteria in the family Actinomycetaceae may play an important role in periodontitis and that measurement of TLA is a clinically reliable marker for the diagnosis of periodontitis in dogs.

Actinomyces↗