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PubMed1982

Marrow microenvironment transfer by heterotopic transplantation of freshly isolated and cultured cells in porous sponges.

Cells for study were obtained from mouse bone marrow by three methods: 1) Mechanical dissociation; 2) Trypsin release; and 3) Passage through a syringe and needle. The ratio of the fibroblast colony-forming cells in these suspensions was, respectively, 1:13:1. Of the colonies formed by cells obtained by trypsin release, 70% were alkaline phosphatase positive fibroblasts. Freshly isolated marrow cells and cells harvested, by trypsin treatment, from cultures established from the differently obtained bone marrow cell suspensions, were absorbed into porous sponges for transplantation under the renal capsule of mice. Ossicles containing bone marrow formed after transplantation of either freshly isolated cells, or cultured cells obtained from marrow by trypsin treatment, but not after the transplantation of cells cultured from marrow obtained by mechanical dissociation. The size of the bone marrow "organs" that formed was determined by both the number of cells grafted and the size of the sponge in which they had been absorbed for grafting.

Alkaline Phosphatase↗
PubMed1984

[Quantitative characteristics of the transfer of the hematopoietic microenvironment].

Heterotopic transplantation of marrow fragments results in the formation of new bone marrow organs. The amount of hemopoietic cells populating these organs is connected nonlinearly with the volume of the grafted tissue or with the number of transplanted marrow cells. Statistical analysis points out that the number of the populating cells depends on the radius of the initial bone marrow transplant. Thus, the previous data on the radiosensitivity of the microenvironmental transfering stromal cells and on their concentration obtained by measuring the size of the heterotopic bone marrow organs have turned out incorrect.

Animals↗
PubMed1984

[Changes in the microenvironment of the maturing bovine oocyte in vivo].

The development of pre-ovulatory bovine follicles from the onset of oestrus until ovulation may be divided into four phases on the basis of the patterns of the steroid levels in the follicular fluid. Changes in the morphology of the follicular wall and the oocyte coincide with these patterns. Prior to the pre-ovulatory LH surge steroid synthesis is directed towards the production of oestradiol-17 beta, and androstenedione is the major substrate of aromatase. After the LH peak, steroid synthesis switches to the production of progesterone shortly prior to ovulation. The membrana granulosa cell-layer shows morphological luteinization at the time. The elevated levels of LH and FSH observed in the follicular fluid suggest that changes in the micro-environment of the maturing bovine oocyte are regulated by these gonadotrophins.

Androstenedione↗