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Addressing global warming and biodiversity through forest restoration and coastal wetlands creation

The Climate Challenge is a partnership between the Department of Energy and the electric utility industry to reduce, avoid, and sequester greenhouse gases. A portion of the initiative, the sequestration of greenhouse gases, is the focus of this presentation. Over 4 million acres of bottomland hardwood forests were cleared for agriculture in the Mississippi River Valley in the 1970s. Reestablishing these forests would improve depleted wildlife habitats, serve as wildlife corridors, increase biodiversity, and decrease soil erosion. Louisiana is losing coastal wetlands at a rate of approximately 25 square miles/year. This coastal erosion is due to a number of factors and many efforts are currently underway to address the matter. One such effort is the use of material generated in the dredging of navigational canals; however, this material is low in nutrient value, making the regeneration of marsh grasses more difficult. In addition, bottomland hardwood forests and coastal wetland grasses are excellent 'carbon sinks' because they take carbon dioxide out of the atmosphere and store it in living plant tissue. Entergy Services, Inc. is an electric utility with a service territory that comprises portions of both the Lower Mississippi River Valley and the Gulf of Mexico coastline. This provides an opportunity to positively address both habitat losses noted above while at the same time addressing global warming, forest fragmentation, and biodiversity. Entergy, through its affiliation with the UtiliTree Carbon Company, is participating in projects that will investigate the feasibility of using bottomland hardwood reforestation on cleared marginal farmlands now managed by the Louisiana Department of Wildlife and Fisheries and the US Fish and Wildlife Service. Entergy has also begun a research project with the Environmental Protection Agency and the State of Louisiana. The research is a compost demonstration project that will utilize wood waste generated through our tree-trimming program as a compost material that will be mixed with low nutrient dredge material to create new coastal wetlands. Taken together, Entergy's initiatives will be able to address global warming through carbon sequestration, restore fragmented forest habitats, reduce coastal erosion and improve the quality of a vital coastal aquatic nursery habitat. Efforts will be made to manage the created habitats for biodiversity. Pulling all these ideas together creates an effect in which the whole is greater than the sum of the parts. In such a synergy of ideas, there are no losers and the winners are both industry participants and the environment.

Journal Article↗

Atmospheric nitrogen in the Mississippi River Basin--emissions, deposition and transport.

Atmospheric deposition of nitrogen has been cited as a major factor in the nitrogen saturation of forests in the north-eastern United States and as a contributor to the eutrophication of coastal waters, including the Gulf of Mexico near the mouth of the Mississippi River. Sources of nitrogen emissions and the resulting spatial patterns of nitrogen deposition within the Mississippi River Basin, however, have not been fully documented. An assessment of atmospheric nitrogen in the Mississippi River Basin was therefore conducted in 1998-1999 to: (1) evaluate the forms in which nitrogen is deposited from the atmosphere; (2) quantify the spatial distribution of atmospheric nitrogen deposition throughout the basin; and (3) relate locations of emission sources to spatial deposition patterns to evaluate atmospheric transport. Deposition data collected through the NADP/NTN (National Atmospheric Deposition Program/National Trends Network) and CASTNet (Clean Air Status and Trends Network) were used for this analysis. NOx Tier 1 emission data by county was obtained for 1992 from the US Environmental Protection Agency (Emissions Trends Viewer CD, 1985-1995, version 1.0, September 1996) and NH3 emissions data was derived from the 1992 Census of Agriculture (US Department of Commerce. Census of Agriculture, US Summary and County Level Data, US Department of Commerce, Bureau of the Census. Geographic Area series, 1995:1b) or the National Agricultural Statistics Service (US Department of Agriculture. National Agricultural Statistics Service Historical Data. Accessed 7/98 at URL, 1998. http://www.usda.gov/nass/pubs/hisdata++ +.htm). The highest rates of wet deposition of NO3- were in the north-eastern part of the basin, downwind of electric utility plants and urban areas, whereas the highest rates of wet deposition of NH4+ were in Iowa, near the center of intensive agricultural activities in the Midwest. The lowest rates of atmospheric nitrogen deposition were on the western (windward) side of the basin, which suggests that most of the nitrogen deposited within the basin is derived from internal sources. Atmospheric transport eastward across the basin boundary is greater for NO3- than NH4+, but a significant amount of NH4+ is likely to be transported out of the basin through the formation of (NH4)2SO4 and NH4NO3 particles--a process that greatly increases the atmospheric residence time of NH4+. This process is also a likely factor in the atmospheric transport of nitrogen from the Midwest to upland forest regions in the North-East, such as the western Adirondack region of New York, where NH4+ constitutes 38% of the total wet deposition of N.

Air Pollutants↗

Calcium nephrolithiasis: effect of water hardness on urinary electrolytes.

OBJECTIVES: To analyze the impact of water hardness from public water supplies on calcium stone incidence and 24-hour urine chemistries in patients with known calcium urinary stone formation. Patients are frequently concerned that their public water supply may contribute to urinary stone disease. Investigators have documented an inverse relationship between water hardness and calcium lithogenesis. Others have found no such association. METHODS: Patients who form calcium stones (n = 4833) were identified geographically by their zip code. Water hardness information from distinct geographic public water supplies was obtained, and patient 24-hour urine chemistries were evaluated. Drinking water hardness was divided into decile rankings on the basis of the public water supply information obtained from the Environmental Protection Agency. These data were compared with patient questionnaires and 24-hour urine chemistries. The calcium and magnesium levels in the drinking water were analyzed as independent variables. RESULTS: The number of total lifetime stone episodes was similar between patients residing in areas with soft public water and hard public water. Patients consuming the softest water decile formed 3.4 lifetime stones and those who consumed the hardest water developed 3.0 lifetime stones (P = 0.0017). The 24-hour urine calcium, magnesium, and citrate levels increased directly with drinking water hardness, and no significant change was found in urinary oxalate, uric acid, pH, or volume. CONCLUSIONS: The impact of water hardness on urinary stone formation remains unclear, despite a weak correlation between water hardness and urinary calcium, magnesium, and citrate excretion. Tap water, however, can change urinary electrolytes in patients who form calcium stones.

Adolescent↗

A United States regulator's perspective on the ongoing chlorofluorocarbon transition.

The Food and Drug Administration (FDA) put in place a general ban on the use of chlorofluorocarbons for the products it regulates (medical devices, drugs, and foods) in 1978, exempting those products where chlorofluorocarbon use was determined to be essential for the public health. In the intervening years, as the international commitment to a full transition away from all chlorofluorocarbon use took shape under the Montreal Protocol, the FDA has worked with industry to facilitate the development and testing of alternative technologies and products for inhalation drug products. As these alternative products begin to move from testing through the approval process and into marketing, the FDA is working collaboratively with the Environmental Protection Agency, other governmental agencies, and nongovernmental stakeholders to develop a transition policy for the United States. The transition policy for metered dose inhalers must be one that achieves the dual aims of first protecting the patients who rely on these vital medical products, while also achieving the public health need of protecting the ozone layer. As a part of developing such a transition strategy, the FDA published an advance notice of proposed rulemaking (ANPRM) in March 1997. The ANPRM proposed mechanisms by which the FDA could determine when chlorofluorocarbon use in a drug product could no longer be considered essential. The ANPRM resulted in a large amount of valuable public debate and input. The FDA is now working to incorporate the knowledge gained from these public comments as it continues the rule-making process.

Chlorofluorocarbons↗

The toxicity of fluoranthene to Hyalella azteca in sediment and water-only exposures under varying light spectra.

In the US Environmental Protection Agency methods for sediment toxicity testing, the light regimen is specified as a 16:8 light dark cycle with 500-1000 lx. The potential for photoinduced toxic effects from this requirement is evaluated. Hyalella azteca were exposed to fluoranthene in both water only and sediment to examine the impact of light spectra on the toxicity of fluoranthene. The light sources included gold fluorescent light (lambda > 500 nm), cool white fluorescent light, and UV-enhanced fluorescent light. Toxicity was determined as mortality after 10 days of exposure. The extent of mortality was determined both as LC(50) and LR(50) (median lethal body residue). In water-only exposures, the toxicity of fluoranthene was greatest under the UV-enhanced spectra, followed by fluorescent light, and least toxic under the gold light. Both the LC(50) and LR(50) values exhibited the same pattern. The toxicity under gold light gave an LR(50) of 0.81 mmol kg(-1) (0.82-0.79, 95% CI) similar to values expected for the acute toxicity of nonpolar narcotic (anesthetic) compounds. The LR(50) values under the other two light sources were substantially lower, 4 and 58 times lower for the fluorescent and UV-enhanced exposures, respectively. In sediment, toxicity was not significantly affected by the light source. Toxicity occurred only when the body residue concentration approached that of the LR(50) under gold light from the water-only exposures. Thus, H. azteca were significantly protected from the light by burrowing into the sediment.

Amphipoda↗

Predicted mercury concentrations in hair from infant immunizations: cause for concern.

Mercury (Hg) is considered one of the worlds most toxic metals. Current thinking suggests that exposure to mercury occurs primarily from seafood contamination and rare catastrophic events. Recently, another common source of exposure has been identified. Thimerosal (TMS), a preservative found in many infant vaccines, contains 49.6% ethyl mercury (EtHg) by weight and typically contributes 25 microg of EtHg per dose of infant vaccine. As part of an ongoing review, the Food and Drug Administration (FDA) announced in 1999 that infants who received multiple TMS-preserved vaccines may have been exposed to cumulative Hg in excess of Federal safety guidelines. According to the centers for disease control (CDC) recommended immunization schedule, infants may have been exposed to 12.5 microg Hg at birth, 62.5 microg EtHg at 2 months, 50 microg EtHg at 4 months, 62.5 microg EtHg at 6 months, and 50 microg EtHg at approximately 18 months, for a total of 237.5 microg EtHg during the first 18 months of life, if all TMS-containing vaccines were administered. Neurobehavioral alterations, especially to the more susceptible fetus and infant, are known to occur after relatively low dose exposures to organic mercury compounds. In effort, to further elucidate the levels of ethyl mercury resulting from exposure to vaccinal TMS, we estimated hair Hg concentrations expected to result from the recommended CDC schedule utilizing a one compartment pharmacokinetic model. This model was developed to predict hair concentrations from acute exposure to methymercury (MeHg) in fish. Modeled hair Hg concentrations in infants exposed to vaccinal TMS are in excess of the Environmental Protection Agency (EPA) safety guidelines of 1 ppm for up to 365 days, with several peak concentrations within this period. More sensitive individuals and those with additional sources of exposure would have higher Hg concentrations. Given that exposure to low levels of mercury during critical stages of development has been associated with neurological disorders in children, including ADD, learning difficulties, and speech delays, the predicted hair Hg concentration resulting from childhood immunizations is cause for concern. Based on these findings, the impact which vaccinal mercury has had on the health of American children warrants further investigation.

Forecasting↗

Inactivation of rotavirus by new polymeric water disinfectants.

Two new insoluble polymeric materials were evaluated for their efficacies in inactivating rotavirus in flowing water in a biocidal filter application. The two polymers are N-chloro and N-bromo derivatives of a poly-styrene hydantoin prepared from commercial poly-styrene. The studies were conducted for rotavirus in halogen demand-free water at pH 7.0, 25 degrees C and Environmental Protection Agency (EPA) Test Water no. 2 at pH 9.0, 4 degrees C which contained heavy halogen demand. The range of flow rates studied was 0.16-1.22 ml s-1 corresponding to contact times in the range of 4-24 s. Both of the polymers were effective in inactivating rotavirus, the N-bromo derivative providing a 4-6 log reduction under the test conditions. The materials may be useful as supplemental filters for hand-held water purification units.

Disinfectants↗

The saturated zone at Yucca Mountain: an overview of the characterization and assessment of the saturated zone as a barrier to potential radionuclide migration.

The US Department of Energy is pursuing Yucca Mountain, Nevada, for the development of a geologic repository for the disposal of spent nuclear fuel and high-level radioactive waste, if the repository is able to meet applicable radiation protection standards established by the US Nuclear Regulatory Commission and the US Environmental Protection Agency (EPA). Effective performance of such a repository would rely on a number of natural and engineered barriers to isolate radioactive waste from the accessible environment. Groundwater beneath Yucca Mountain is the primary medium through which most radionuclides might move away from the potential repository. The saturated zone (SZ) system is expected to act as a natural barrier to this possible movement of radionuclides both by delaying their transport and by reducing their concentration before they reach the accessible environment. Information obtained from Yucca Mountain Site Characterization Project activities is used to estimate groundwater flow rates through the site-scale SZ flow and transport model area and to constrain general conceptual models of groundwater flow in the site-scale area. The site-scale conceptual model is a synthesis of what is known about flow and transport processes at the scale required for total system performance assessment of the site. This knowledge builds on and is consistent with knowledge that has accumulated at the regional scale but is more detailed because more data are available at the site-scale level. The mathematical basis of the site-scale model and the associated numerical approaches are designed to assist in quantifying the uncertainty in the permeability of rocks in the geologic framework model and to represent accurately the flow and transport processes included in the site-scale conceptual model. Confidence in the results of the mathematical model was obtained by comparing calculated to observed hydraulic heads, estimated to measured permeabilities, and lateral flow rates calculated by the site-scale model to those calculated by the regional-scale flow model. In addition, it was confirmed that the flow paths leaving the region of the potential repository are consistent with those inferred from gradients of measured head and those independently inferred from water-chemistry data. The general approach of the site-scale SZ flow and transport model analysis is to calculate unit breakthrough curves for radionuclides at the interface between the SZ and the biosphere using the three-dimensional site-scale SZ flow and transport model. Uncertainties are explicitly incorporated into the site-scale SZ flow and transport abstractions through key parameters and conceptual models.

Forecasting↗

DERM/INFONET: a concept becomes a reality.

The DERMatology INFOrmation NETwork (DERM/INFONET) of the American Academy of Dermatology has become a reality. DERM/INFONET consists of a number of data bases providing information and educational programs for the dermatologist. Currently the components are: DERM/MLS (Medical Literature Search), DERM/RX (dermatologic therapy), DERM/USP (United States Pharmacopeia data base), DERM/ALLERGENS (Food and Drug Administration and Environmental Protection Agency Listings of allergens); Melanoma Prognosis Model; Electronic Mail; Bulletin Board; Meetings Calendar; ICD/CPT (International Classification of Diseases/Current Procedural Terminology) codes; AAD Membership/Committee Directories; and Dermatology Quiz. Additional data bases are planned. As audiovisual and alphanumeric communication systems evolve, newer opportunities for enhancing the DERM/INFONET Biomedical Communication Network will undoubtedly provide even greater opportunities for aiding the dermatologist in delivering state-of-the art management for their patients.

Computer Communication Networks↗

Insect repellents: an overview.

The optimal management of arthropod bites is prevention, and many over-the-counter insect repellents are available. Since first marketed in 1956, deet has remained the most effective repellent against mosquitoes, biting fleas, gnats, and chiggers. Permethrin is applied to clothing rather than to skin, and it is a better repellent against ticks than deet. The risk of serious side effects with the use of deet is slight; nevertheless, the lowest effective concentration should be used. The current, popular repellent agents (for adults and children) and their active ingredients are discussed. In addition, the Environmental Protection Agency guidelines for the safe use of insect repellents are supplied.

Adult↗

Night of the sirens: analysis of carbon monoxide-detector experience in suburban Chicago.

STUDY OBJECTIVE: To determine the pattern and environmental causes of carbon monoxide (CO)-detector alarms. METHODS: Data including time, location, detector manufacturer, CO measurements in the home, reported illness, cause, and actions taken were collected between July 15, 1994, and January 26, 1995, on all calls to 17 suburban Chicago fire departments for CO-detector alarms. We used univariate time-series analysis involving joint estimation of model parameters and outlier effects to analyze data and compared data on ambient CO levels from the Illinois Environmental Protection Agency to the number of calls per day. RESULTS: During the study period, 777 calls for sounding CO detectors were made to the fire departments in question. The median number of calls per day was three. Our univariate time series identified 3 days with a significant excess of calls (December 12, 29 calls; December 21, 69; December 22, 128; P < .001). The average ambient CO readings on these days were 0.99, 3.25, and 3.89 ppm, respectively, compared with an overall mean of 8.8 ppm. In-home CO levels among all 828 measurements taken from the 777 domestic calls ranged from 0 to 425 ppm, 0 in 249 (30%), 1 to 10 in 340 (41%), 11 to 50 in 149 (18%), 51 to 100 in 22 (9%), and more than 100 in 11 (1.3%). No measurement was taken in six cases. Cause of alarm was listed as furnace in 25 cases, auto exhaust in 24, stove/oven in 22, poor location of detector in 14, water heater in 11, outside sources in 7, and multiple sources in 7. Other sources accounted for fewer than 1% each. The participating fire departments considered 242 cases (31%) to be false alarms. Cause was not determined in 400 calls (51%). In 37 calls (4.8%), people reported illness. CONCLUSION: Above-average ambient CO levels coincided with a significant increase in the number of calls and may have contributed to the triggering of CO alarms.

Air Pollution, Indoor↗

Efficacy of thermal treatment and copper-silver ionization for controlling Legionella pneumophila in high-volume hot water plumbing systems in hospitals.

BACKGROUND: Thermal treatment and copper-silver ionization are often used for controlling Legionella pneumophila in high-volume hospital plumbing systems, although the comparative efficacies of these measures in high-volume systems are unknown. METHODS: Thermal treatment of a hot water circuit was accomplished by flushing hot water (> 60 degrees C) through distal fixtures for 10 minutes. Copper-silver ionization was conducted in three circuits by installing units into return lines immediately upstream from hot water tanks. Recovery rates of L. pneumophila were monitored by culturing swab samples from faucets. Concentrations of copper and silver in water samples were determined by atomic absorption spectrophotometry. RESULTS: Four heat-flush treatments failed to provide long-term control of L. pneumophila. In contrast, ionization treatment reduced the rate of recovery of L. pneumophila from 108 faucets from 72% to 2% within 1 month and maintained effective control for at least 22 months. Only three samples (1.9%) of hot water from faucets exceeded Environmental Protection Agency standards for silver, and none exceeded the standards for copper. Of 24 samples obtained from hot water tanks, 42% and 50% exceeded the silver and copper standards, respectively. CONCLUSIONS: Copper-silver ionization effectively controls L. pneumophila in high-volume plumbing systems and is superior to thermal treatment; however, high concentrations of copper and silver can accumulate at the bottom of hot water tanks.

Copper↗

[Trihalomethanes in the drinking water of Carabobo State, Venezuela].

OBJECTIVE: Disinfection of water with chlorine in water treatment plants leads to the formation of trihalomethanes (THMs). These compounds are associated with adverse health effects. The aim of this study was to analyze THM concentrations in the water provided for human consumption in the two main water treatment systems of Carabobo State: the Alejo Zuloaga plant and the Pao-Cachinche reservoir, which form the Central Regional System I (CRS I), and the Lucio Baldo Soules plant and the Pao-La Balsa reservoir, which form the Central Regional System II (CRS II). METHODS: We analyzed 144 water samples collected in 6 samplings carried out in 2000 and 2001. THM concentrations were determined by gas chromatography using the headspace technique. The concentrations of the following THMs were measured: chloroform (CHCl3), bromoform (CHBr3), chlorodibromomethane (CHBr2Cl) and bromodichloromethane (CHCl2Br). RESULTS: The concentration of total THMs was between 47.84 g/l and 94.23 g/l. CHCl3 was the most commonly formed compound representing 83% of all THMs in the CRS I and 82% in the CRS II. The concentrations of total THMs in the CRS I, specifically in the Baja and San Diego networks, were significantly higher (p < 0.05) than permissible levels set by the American Environmental Protection Agency (80 g/l) for the sum of all four THMs. CONCLUSIONS: The results show that in the area studied there is a risk of adverse health effects due to THMs in drinking water, especially in the Baja and San Diego networks.

Chlorine↗

PM10 pollution episodes as a function of synoptic climatology in a mountainous industrial area.

In this study an attempt is made to investigate the prevailing meteorological conditions during days with high concentrations of PM10 (particles with diameter < 10 microns), collected in the Eordea mountain basin. This is an industrial area in the northwestern mountainous region of Greece. Over the 4-year data-gathering period, the days in which the United States Environmental Protection Agency 24-h PM10 standard was exceeded (episode days), were identified in relation to prevailing synoptic scale and local meteorological conditions. The results indicated that days with increased PM10 concentrations in this area can be grouped into four categories in relation to their synoptic circulation characteristics. The highest concentrations were found to be associated with stagnant conditions. Under these conditions, local circulations developed in the area, resulting in recirculation and accumulation of pollutants.

Air Pollutants↗

The effect of the 14-day agricultural restricted entry interval on azinphosmethyl exposures in a group of apple thinners in Washington state.

We examined the effect of the 14-day agricultural restricted entry period on absorbed pesticide doses in a group of twenty experienced apple thinners. Thinners entered orchards 1-49 days following azinphosmethyl applications. Urine samples (n=296) collected throughout the thinning season were analyzed for the three dialkylphosphate metabolites of azinphosmethyl to estimate absorbed daily doses. Separate dose distributions were created for samples collected when the interval was <14 days, or 14 days or more; geometric mean doses for these two categories differed by a factor of two (42 and 19 microg/kg/day, respectively; p<0.0001). Dose estimates were compared to US Environmental Protection Agency and California EPA regulatory guidance values for occupational azinphosmethyl risk. None of the doses exceeded the U.S. EPA NOAEL (560 microg/kg/day), but nearly all had a margin of exposure of less than 100. Addition of a 10-fold uncertainty factor to California EPAs NOAEL produced a guidance value of 75 microg/kg/day. Only 2.4% of the doses exceeded this value for re-entry intervals 14 days or more, while 27% exceeded the value for re-entry intervals <14 days. We conclude that the 14-day restricted entry interval provides an appropriate level of worker health protection under the field conditions studied.

Agriculture↗

Regulation of occupational carcinogens under OSHA's Air Contaminants Standard.

We compare the information used by the Occupational Safety and Health Administration (OSHA) to regulate carcinogens under its 1989 Air Contaminants Standard to publicly available information on substances with potential carcinogenic activity. Carcinogenicity evaluations were obtained from the National Institute for Occupational Safety and Health (NIOSH), the American Conference of Governmental Industrial Hygienists (ACGIH), the National Toxicology Program (NTP), the Environmental Protection Agency (EPA), and the International Agency for Research on Cancer (IARC). We focus on three sets of substances: those which were regulated as carcinogens by OSHA in the Standard, those which were included in the Standard but whose exposure limits are based on noncarcinogenic effects, and those substances designated as potential carcinogens by NIOSH, ACGIH, and/or NTP but which were excluded from the Standard. The data indicate that OSHA relied almost exclusively upon the recommendations of the nongovernmental ACGIH to the exclusion of IARC and the three governmental bodies. Given their statutory authority to evaluate chemical carcinogenicity for regulatory agencies such as OSHA, the exclusion of NIOSH and NTP is particularly striking.

Air Pollutants, Occupational↗

In vivo MRI measurements of tumor growth induced by dichloroacetate: implications for mode of action.

Dichloroacetate (DCA) is an important by-product of the chlorination of drinking water that produces liver cancer in rodents. Assessment of the risk that results from concentrations that occur in drinking water will be dependent upon the mode of action held responsible for these tumors. A study by Stauber and Bull [Stauber, A.J. and Bull, R. J (1997) Differences in phenotype and cell replicative behavior of hepatic tumors inducted by dichloroacetate (DCA) and trichloroacetate (TCA). Toxicol. Appl. Pharmacol. 144, 235-246] in mice treated with DCA demonstrated a lesion distribution that was skewed towards many small, altered foci of cells that are assumed to be precursor lesions [EPA, (1996). U.S. Environmental Protection Agency: Proposed Guidelines for carcinogen risk assessment; notice. Fed. Reg. 61, pp. 17960-10811]. The present study was designed to determine the extent to which the tumorigenic effects of DCA could be explained by its effect on tumor growth rates (i.e. tumor promoting activity). In vivo magnetic resonance imaging (MRI) allowed accurate determination of growth rates of individual lesions in mice that had been treated with DCA in drinking water at 2 g/l. Out of thirty treated mice, ten were found to have hepatic tumors detectable by MRI at 48 weeks of treatment. These tumor-bearing animals were assigned to two groups matched on the size of lesions observed by in vivo MR1. Treatment with DCA continued in one group of five mice and was stopped in the other. For both groups, tumor growth rates were determined by measuring changes in size of all lesions greater than 1 mm(3) in volume during a 14-day period. Removal of DCA treatment resulted in growth rates that could not be distinguished from zero across all lesion sizes represented in the sample. These data are in agreement with previous observations of DCAs effects on replication rates within tumors (Stauber and Bull, (1997)). Tumor growth rates observed in animals maintained on treatment decreased with lesion volume in a manner that is consistent with a stochastic Gompertz birth-death process proposed by Tan [Tan, W.Y. (1986) A stochastic Gompertz birth-death process. Stat. Prob. Lett. 4, 25-28]. Parameters of this model obtained by fitting measured growth rates were used to predict the lesion-size distribution expected after one year of DCA treatment. The shape of the predicted lesion-size distribution was similar to that observed by Stauber and Bull (Stauber and Bull, (1997)) in mice sacrificed after 40 weeks of DCA treatment. We conclude that the effects of DCA on the division and/or death rates of spontaneously initiated cells can account for the predominance of small lesions in DCA-treated animals.

Animals↗

Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment.

The Food Quality Protection Act (FQPA) of 1996 requires the United States Environmental Protection Agency to consider the cumulative effects of exposure to pesticides having a 'common mechanism of toxicity.' This paper reviews the information available on the acute neurotoxicity and mechanisms of toxic action of pyrethroid insecticides in mammals from the perspective of the 'common mechanism' statute of the FQPA. The principal effects of pyrethroids as a class are various signs of excitatory neurotoxicity. Historically, pyrethroids were grouped into two subclasses (Types I and II) based on chemical structure and the production of either the T (tremor) or CS (choreoathetosis with salivation) intoxication syndrome following intravenous or intracerebral administration to rodents. Although this classification system is widely employed, it has several shortcomings for the identification of common toxic effects. In particular, it does not reflect the diversity of intoxication signs found following oral administration of various pyrethroids. Pyrethroids act in vitro on a variety of putative biochemical and physiological target sites, four of which merit consideration as sites of toxic action. Voltage-sensitive sodium channels, the sites of insecticidal action, are also important target sites in mammals. Unlike insects, mammals have multiple sodium channel isoforms that vary in their biophysical and pharmacological properties, including their differential sensitivity to pyrethroids. Pyrethroids also act on some isoforms of voltage-sensitive calcium and chloride channels, and these effects may contribute to the toxicity of some compounds. Effects on peripheral-type benzodiazepine receptors are unlikely to be a principal cause of pyrethroid intoxication but may contribute to or enhance convulsions caused by actions at other target sites. In contrast, other putative target sites that have been identified in vitro do not appear to play a major role in pyrethroid intoxication. The diverse toxic actions and pharmacological effects of pyrethroids suggest that simple additivity models based on combined actions at a single target are not appropriate to assess the risks of cumulative exposure to multiple pyrethroids.

Animals↗