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Results for “STREPTODORNASE AND STREPTOKINASE”

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1,735 records · Page 97Linked to original sources
PubMed1979

Histamine-releasing activity (HRA). I. Production by mitogen- or antigen-stimulated human mononuclear cells.

Supernatants from 1- to 2-day cultures of human mononuclear cells induced the release of histamine from basophils. Generation of this histamine-releasing activity (HRA) was stimulated by addition of concanavalin A to the cell cultures. Mononuclear cells were also cultured with SKSD and Candida albicans antigens. Stimulation of HRA production by these antigens was correlated with positive delayed skin reactions. Serial dilutions of supernatants assayed for HRA provided a semiquantitative determination of the level of HRA in mitogen- or antigen-stimulated samples. Antigen increased HRA production when added during the first or second day of culture. Generation of HRA probably requires active protein synthesis, since puromycin was inhibitory, and since preformed HRA could not be recovered from lysed cells. HRA was detected in supernatants after 4 hr, and the effects of antigen stimulation were apparent after 8 hr of culture. Replacement of supernatants with fresh culture medium allowed continued synthesis of substantial quantities of HRA during the second day of culture. A linear correlation was observed between the amount of HRA produced and the mononuclear cell concentration. Our findings provide evidence for the interaction of lymphocytes and basophils via a soluble mediator.

Antigens↗
PubMed1977

The delayed hypersensitivity response: application in clinical surgery.

Delayed hypersensitivity skin testing was performed on 520 surgical patients. Significantly higher incidences of sepsis and mortality (p less than 0.001) were found in the abnormal patients as compared to normal responders in the preoperative (322 patients), postoperative and post-trauma (115 patients), and nonoperative (83 patients) groups. Sequential testing in individual patients was of even greater prognostic value. Of the 177 patients who either remained normal or whose responses became normal, the sepsis rate was 10.1%, and the mortality rate was 8.4%. However, a sepsis rate of 57.6% and a 78% mortality rate were found in those patients who developed abnormal responses or whose responses did not improve. Cancer and increased age (older than 80 years) did not account for the incidence of anergy and relative anergy. The mortality rate was higher in the cancer group. Anergy and relative anergy were found to be associated with malnutrition, sepsis, shock, and trauma. In the clinical setting, effective treatment of these associated conditions, especially the maintenance of body cell mass by the use of total parenteral nutrition, was associated with reversal of the anergic state and an improved prognosis.

Adolescent↗
PubMed1976

Modulating effects of levamisole (NSC-177023) on human lymphocyte response in vitro.

Human lymphocytes were cultured in vitro with multiple concentrations of antigen, mitogen, and allogeneic cells. The addition of levamisole to stimulated cultures in concentrations of 0.03-33 mug/ml significantly amplified the response of lymphocytes to these stimuli with little or no effect on control cultures. The combination of stimulus and levamisole concentration which produced amplification was unique for each individual culture. The inconsistent results found here and elsewhere may reflect instability of levamisole under conditions of the in vitro lymphocyte culture system.

Antigens↗
PubMed1984

[Prevention of postoperative adhesions--animal experiment study].

Standardised lesions were placed on both uterine horns and on the abdominal wall of seventy-five female rabbits. The animals were divided into five groups consisting of 15 animals each. Before closure of the peritoneum the following drugs were applied in the peritoneal cavity: Group I received NaCl (control), Group II received 10 ml poly-(1-vinyl-1-pyrrolidone)-iodine complex (Betaisodona), Group III received 100 000 I. U. streptokinase and 25 000 I. U. streptodornase (Varidase), Group IV received 100 000 KIE approtinine (AntagosanA) and Group V received triamcinolone acetonide (Volon A-Haftsalbe). Two weeks after surgery the animals were relaparotomised and the situs was inspected. Quality and extent of the adhesions were assessed and documented. The results obtained in group II did not differ from controls, while the remaining groups showed significantly fewer adhesions. The best results were obtained in animals receiving Volon A-Haftsalbe (Group V) where 86% fewer adhesions were observed.

Abdomen↗
PubMed1981

[Influence of physical parameters on the production of streptococcal extracellular proteins in cultures with stabilized pH. I. Communication: pH-dependence of extracellular protein production (author's transl)].

The influence of the pH of the medium on growth and production of extracellular proteins was examined in a group C streptococcus (SA. equisimilis strain H 46 A). The pH values employed ranged from 5.0 to 8.5 and the analytical methods used included the determination of enzyme activities, isoelectric focusing, crossed-immunoelectrophoresis, and SDS electrophoresis. The strain is able to grow in the entire pH range used, with maximum growth occurring at pH 6.5. The amount of extracellular proteins elaborated depended on both the pH of the medium and the biomass produced. A specific pH range was defined for optimum production of all enzymes examined. With the exception of streptodornase and NAD-glycohydrolase, the pH optimum for product formation was generally in the neutral and weakly alkaline range. Active proteinase was formed only a pH 6.0, leading to gross degradation of most of the other proteins in the culture medium. At pH values ranging from 6.5 to 7.5, isoelectric focusing revealed the production of 28 different extracellular proteins, and crossed immunoelectrophoresis demonstrated 30 to 32 antigens. The number of extracellular products produced greatly declined at pH values above and below this range. Under optimum conditions, the main extracellular product at H 46 A was streptokinase, followed by deoxyribonuclease A.

Antigens, Bacterial↗