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PubMed1981

The effect of calfhood vaccination with strain 19 on the serological diagnosis and eradication of bovine brucellosis.

Data from 42,224 cattle from 694 herds collected during the brucellosis eradication campaign were used to examine the effects of calfhood strain 19 vaccination. The prevalence to infection in vaccinated herds was 1.8% compared with 9.1% in non-vaccinated herds (p less than 0.005). The mean titre in the former group was lower (p less than 0.001). Vaccinated herds required 3.3 herd tests to achieve a provisionally free status compared with 4.8 in non-vaccinated herds (0.001 less than p less than 0.005). Vaccination did not significantly reduce the number of herd test in herds with less than 100 breeding females. In tests after the initial herd test only 0.5% reactors were found in vaccinated herds compared with 6.9% in non-vaccinated herds (p less than 0.005). There were 0.9% false positive to the Rose Bengal plate test in non-vaccinated and 2.1% in vaccinated animals (p less than 0.005) in non-infected herds. In infected herds this percentage was 3.0% and 4.2% respectively by (p less than 0.05). In the non-infected herds there were 0.04% false positives to the complement fixation tests out of 10,506 non-vaccinated cattle tested and 0.2% out of 24,734 vaccinated cattle.

Animals↗
PubMed1985

Comparisons of immunological tests for serodiagnosis of Chagas disease in Bolivian patients.

Enzyme linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were evaluated and compared to the classical immunofluorescence (IF) and complement fixation test (CFT) in the immunological diagnosis of Chagas' disease, using 407 sera from Bolivian patients. 72.7 to 79.5% of randomised sera, coming from patients living in endemic areas for Chagas' disease were considered as positive, according to the test limits, previously determined. The techniques could be classified according to their percentage detection as ELISA greater than IF greater than CFT greater than IEP. The quantitative correlations between the tests were excellent (p less than 0.001). 92.8% of the sera were positive or negative for the four tests, 6.1% for three tests and 1.1% for only two tests. The agreement between the tests ranged from 94.6 to 99.2%, co-positivity from 95.5 to 100% and co-negativity from 88.5 to 100%. IF gave the best results, and could be considered as the reference test since it was easy and rapid to perform. However to avoid errors or discrepancies between laboratories, two tests, such as IF and CFT, might be associated. ELISA can be used if higher sensitivity is required. IEP showed 1 to 14 precipitation bands in 96% of the sera from infected patients. The precipitation band 5, previously demonstrated as Trypanosoma cruzi specific, was present in 73% of these sera, indicating the interest to use immunoprecipitation test, if more specificity is required for the immunodiagnosis of Chagas' disease.

Bolivia↗
PubMed1985

The isolation of Kemerovo group orbiviruses and Uukuniemi group viruses of the family bunyaviridae from Ixodes uriae ticks from the Isle of May, Scotland.

Viruses isolated from ticks (Ixodes uriae) from a seabird colony on the Isle of May, Scotland, were shown by complement fixation tests to be related to the Uukuniemi and Kemerovo serogroups. Electron microscopic studies on the Uukuniemi viruses showed them to have a morphology characteristic of the bunyaviridae, and the Kemerovo group to be characteristic of orbiviruses. Separate isolates from the two serogroups were distinguished from each other by neutralization tests.

Animals↗
PubMed1981

[Indirect immunofluorescence and complement absorption test characterization of anti-brain antibodies in schizophrenia].

The content and tropism of anticerebral antibodies were examined in 20 schizophrenic patients using the indirect immunofluorescence and complement fixation tests. With the aid of the latter the antibodies were revealed in 15 patients, the antibodies to the homologous brain being more frequent in patients with a more severe, continuously progressing schizophrenia, while the antibodies to a heterologous (rat) brain were more often detected in patients with slowly progressing forms of the disease. In 18 patients, the indirect immunofluorescence test showed fixation of immunoglobulins on the nervous tissue components, such as, neurons, myelin, vascular walls. Rather frequently fluorescence of the microglia cells was observed: according to literary data this is not the case with sera of neurological patients. Of attention was a selective fluorescence of the canaliculi observed in examinations of the renal tissue. The data obtained point to a participation of the immunological reactions in the schizophrenia pathogenesis, these reactions involving various morphological and functional systems.

Adult↗
PubMed1982

Virus antibodies in the serum of patients with liver disease.

Two hundred and twenty-six patients suspected of having liver disease were examined clinically, by a liver biopsy and laboratory test, according to a prospective scheme. Blood samples obtained just before the liver biopsy were coded and subsequently examined blindly, using the complement fixation test (CFT). The antigens were influenza A and B viruses, parainfluenza 1, 2, and 3, respiratory syncytial viruses, varicella, morbilli, cytomegalo and herpes simplex viruses. The sera were also examined by the CFT against Mycoplasma pneumoniae antigen. Antibodies against rubella virus were determined in a haemolysis-in-gel test. HBsAg and HBsAb were determined by a staphylococcal radioimmunoassay, and sera from patients with chronic active hepatitis (CAH) and chronic persistent hepatitis (CPH) were also examined for antibodies against hepatitis A virus by radioimmunoassay. Highly significant antibody titres against morbilli virus were found in patients with CAH and CPH. Patients with CPH or liver cirrhosis also had significantly higher titres against rubella virus than other groups. Some patients with liver granulomas had high titres against rubella virus. Only in one patient with CAH was a positive test for HBsAg found, and in one a positive test for HBsAb. Seven patients in the CAH and CPH groups had very high titres against both rubella and morbilli viruses.

Adolescent↗
PubMed1977

Radioimmunoassay of IgM, IgG, and IgA Brucella antibodies.

A radioimmunoassay (R.I.A.) has been devised to measure the serum antibody against Brucella abortus in each of the immunoglobulin classes IgM, IgG, and IgA. This test was applied to 46 sera from individuals with various clinical types of brucellosis, and the results were compared with the results of conventional direct and indirect agglutination and complement-fixation tests. The R.I.A. provided a highly sensitive primary-type assay which avoided the difficulties with blocking or non-agglutinating antibody, and thus has many advantages in the diagnosis of acute and chronic stages of brucella infection in man. The R.I.A. was successful in detection of antibody in many instances in which conventional serological tests were negative, and such antibody could (if IgM) be associated with acute or (if IgG or IgA) with chronic cases of brucellosis. One case in which B. abortus was isolated by blood culture but which failed to yield antibody by conventional tests, nevertheless showed substantial levels of IgM and IgG antibody by R.I.A. In other cases the R.I.A. test helped to eliminate the diagnosis of brucellosis by revealing absent or low antibody levels.

Acute Disease↗
PubMed1975

A review of routine tests for respiratory viruses in hospital inpatients.

During the period June 1967 to May 1972, viral tests were performed upon 1195 inpatients aged 12 and over in the Brompton Hospital. The overall diagnostic rate was 21-5%, comparing 9-3% by isolation and 14-9% by complement fixation (6-5% by four-fold rises in titre in paired sera, 8-4% by titres larger than or equal to 160 in single sera). Only 42% of all patients had an acute respiratory illness within one week prior to admission, which seriously curtailed the chances of isolating viruses and of obtaining early serological specimens. The value of complement fixation tests on single specimens in the clinical management of patients is discussed. The profound effect of infections by influenza A on respiratory morbidity and hospital admission is stressed.

Adenoviridae↗
PubMed1975

Application of the single radial diffusion test for assay of antibody to influenza type A viruses.

Single radial diffusion (SRD) tests for antibodies to influenza type A hemagglutinin, neuraminidase, nucleoprotein, and matrix protein antigens were compared with conventional hemagglutination inhibition, neuraminidase inhibition, and complement fixation tests. Sera used in this study were obtained in 1968-1969 from volunteers before and after vaccination and before and after an ensuing epidemic of Hong Kong influenza. The SRD test compared favorably with conventional tests for assessment of vaccine- or infection-induced rises in antibody titers to influenza type A viruses. Little linear relationshiip was seen between zone areas with SRD and titers with conventional tests, suggesting that the SRD test may detect antibody of different quality or specificity. The SRD seemed equal to the hemagglutination inhibition test for predicting susceptibility to influenza. SRD is a simple test for the recognition of antibody to various antigenic components of the influenza virus and could prove to be a valuable epidemiological tool.

Antibodies, Viral↗
PubMedDate not supplied

Autoimmunity and absence of rheumatoid factors in experimental Trypanosoma (Trypanozoon) equiperdum infections in the rabbit.

The serum levels of IgM and IgG have been shown to increase approximately 7 to 10 times during the development of Trupanosoma equiperdum infection in the rabbit. After separation of the 7 S and 19 S fractions by density gradient centrifugation, both fractions were found by complement fixation tests to contain anti-trypanosome antibodies. The 19 S fraction also contained autoantibodies reacting with tissue antigens of rabbit liver, kidney and heart. Results of passive haemagglutination and latex fixation tests could not confirm earlier reports of the presence of rheumatoid factors in infected rabbits.

Animals↗
PubMed1985

Cell-mediated and humoral immune response to Mycoplasma hyopneumoniae in pigs enhanced by dextran sulfate.

The effect of dextran sulfate (DS), known to be cytotoxic to macrophages, on the cell-mediated and humoral immune response to nonviable Mycoplasma hyopneumoniae in pigs was investigated. The cell-mediated immune response was determined by means of lymphocyte transformation a test, using uptake of [3H]thymidine in a microculture system and the humoral immune response by means of a microplate complement-fixation test. Peripheral blood lymphocytes from pigs vaccinated with nonviable M hyopneumoniae and DS incorporated substantially more [3H]thymidine than did those from pigs given Mycoplasma or DS alone. The transformation of lymphocytes from M hyopneumoniae-DS vaccinated pigs was enhanced when M hyopneumoniae cells used in the assay system were heated at 60 C for 30 minutes. Similarly prepared M flocculare and M hyorhinis cells also stimulated lymphocytes from M hyopneumoniae-DS vaccinated pigs, but not nearly as great as when M hyopneumoniae cells were used. The humoral antibody response and the cell-mediated immune response to nonviable M hyopneumoniae was markedly enhanced by DS. Pigs were vaccinated with nonviable M hyopneumoniae and/or DS 4 times and challenge exposed intratracheally with viable M hyopneumoniae. Pigs vaccinated with M hyopneumoniae and DS had less severe pneumonia than did nonvaccinated pigs.

Animals↗
PubMed1967

Agar-gel precipitin-inhibition test for coccidioidomycosis. I. Preliminary evaluation of the complement-fixation and agar-gel precipitin tests in the serodiagnosis of human coccidioidomycosis.

The agar-gel precipitin-inhibition serological test for coccidioidomycosis was a more sensitive indicator of Coccidioides immitis antibodies than the tube precipitin, the agar-gel immunodiffusion, in the complement-fixation tests in assaying monkey sera, whether these sera were from prechallenge-vaccinated or postchallenged animals. When applying this technique to the assay of human sera, an analogous finding generally persisted. However, some human sera were positive by the complement-fixation test and negative by the agar-gel precipitin-inhibition test. These sera were diffused in agar-gel against various coccidioidin complement-fixation, tube precipitin, and agar-gel precipitin-inhibition test antigens with essentially negative results.

Animals↗
PubMed1977

Complement-fixing antibody response in pneumococcal pneumonia.

Previous studies of complement-fixing antibodies to pneumococcal capsular polysaccharides in humans have yielded conflicting results. We studied 65 sera from 25 patients with pneumococcal pneumonia, using both fresh sera and heat-inactivated sera with added human complement. Only 4 of the 25 patients developed detectable levels of complement-fixing anticapsular antibody. Of the 25 patients, 22 developed detectable levels of hemagglutinating anticapsular antibody, indicating that they were able to develop an immunological response during the infection. Most of the antibody detected by hemagglutination was sensitive to 2-mercaptoethanol, but some 2-mercaptoethanol-resistant antibody was also detected. In studies with rabbit antiserum, the complement fixation test was found to be as sensitive as the hemagglutination test for detection of anticapsular antibody. It is not clear why detectable levels of complement-fixing antibody do not develop more often in patients with pneumococcal pneumonia. Studies of purified anticapsular antibody would be of interest to determine whether or not these antibodies are restricted to immunoglobulin subclasses having a limited capacity to fix complement.

Adult↗
PubMed1977

An indirect haemagglutination test for demonstration of gonococcal antibodies using gonococcal pili as antigen. I. Methodology and preliminary results.

An indirect haemagglutination (IHA) test for detection of gonococcal antibodies in human sera was established using purified pili as antigen. The majority of sera (44/51), positive in the gonococcal complement fixation test, were also positive in the IHA test with titres within the range 320-10,000. The results obtained for the following three groups of sera indicate a comparatively high speicificity of the IHA test; 1) 52 sera from children below 12 years of age were all negative, 2) 98% (184/188) of sera from blood donors were negative, 3) only sera from three adults out of 21 patients with meningococcal infections gave a positive reaction.

Adult↗
PubMed1979

[Use of the indirect hemagglutination reaction in studying ornithosis infection. III. The diagnostic value of the IHR with an ornithosis erythocytic diagnosticum].

In both experimental and clinical conditions the passive hemagglutination test (PHAT with the use of an ornithosis erythrocytic diagnostic preparation was found to be sufficiently sensitive and specific as compared with the complement fixation test (CFT), a routine testing method. The study of the dynamics of immune response in infected animals and ornithosis patients allowed to regard the PHAT as a comparatively early method of serological analysis. Hemagglutinins were also found to circulate in the patients' blood sera only for a short time (on the average for 1 1/2--2 months). The CFT and the PHAT with erythrocytic diagnostic preparation, used in combination, will make it possible not only to diagnose ornithosis in patient more effectively, but also to differentiate between the cases of infection and anamnestic reaction.20

Animals↗
PubMed1990

[The optimization of immunoenzyme analysis with conjugates of virus-specific antibodies and alkaline phosphatase].

The data obtained in the study of the dependence of sensitivity of enzyme immunoassay (EIA) on chromo- and fluorogenic substrates used in this assay are presented. The sandwich variant of EIA, carried out with the use of antibodies labeled with alkaline phosphatase, has been shown to be 4-170 times, sometimes 500 times, more sensitive (in terms of concentrations at which aphthous fever virus antigens can be detected) than the complement fixation test and 1.8-64 times more sensitive than the passive hemagglutination test.

Alkaline Phosphatase↗
PubMed2003

Serological diagnosis of brucellosis caused by Brucella canis.

Blood serum samples from 2,328 dogs were tested to detect antibodies against Brucella canis with the agar gel immunodiffusion (AGID) and 2-mercaptoethanol slide agglutination test (ME-SAT) using Brucella ovis as the antigen. All blood serum samples were also evaluated for antibodies against Brucella abortus and Brucella melitensis using the Rose Bengal test. Twentyfive (1.07%) of the sera evaluated were considered positive with AGID test. Only 4 (16%) of these blood serum samples were positive when evaluated with ME-SAT. The 25 AGID positive samples and 25 AGID negative serum samples were also examined by: the complement fixation test (CFT) using B. ovis hot saline extract (HSE) as the antigen, indirect enzyme linked immunosorbent assay (ELISA) and immunoblotting (IB) using B. canis and B. ovis HSE antigens. Two positive canine sera from culture positive dogs and the serum of an experimentally RM6/66 B. canis-infected rabbit were employed as positive controls and one serum from a known uninfected dog as a negative control. ELISA with B. canis antigen gave 9 (18%) positive results (6 AGID-positive and 3 AGID-negative sera). ELISA performed with B. ovis antigen detected 15 (30%) positive samples (10 AGID-positive, 5 AGID-negative and 8 B. canis ELISA positive sera). IB analysis of known positive controls sera employing B. canis antigen detected bands with molecular weights of 94-80, 64-50, 35, 32-30, 28, 23, 20-18, 15-12 kDa. The same sera tested with B. ovis antigen revealed bands of 35, 32-30, 25, 23, 20-18, 15-12 kDa. No bands were observed with the negative control serum and the 50 canine tested sera.

Agglutination Tests↗