Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “LEPTOSPIRA”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,729 records · Page 96Linked to original sources
PubMed1992

[Detection of leptospira by dot blot hybridization with photobiotin- and 32P-labelled DNA].

Photobiotin- and 32P-labelled DNA probes of L. interrogans sv. lai strain 017 were produced and the DNA and leptospires were dotted on the NC filter and hybridized. The results showed that photobiotin and 32P-labelled DNA probes could detect DNAs of homology. The smallest amount of DNA that could be detected with the probes were 5pg and 1pg, and the smallest numbers of pathogenic leptospires were 5 x 10(3) and 10(3), respectively. Nonpathogenic leptospires. L. biflexa sv. patoc strain Patoc 1, L. illini strain 3055, Escherichia coli, Bacillus aerogenes capsulatus, and Salmonella anatis, could not be detected by the probes. The study demonstrates that leptospires DNA probe could be produced by labelling the DNA with photobiotin. It has higher sensitivity and specificity and can be used for detection of leptospires in the field and clinic.

Affinity Labels↗
PubMed1992

Typing leptospira from the perspective of a reference laboratory.

Leptospirosis is caused by different leptospiral variants. Analysis by cross agglutination absorption tests (CAAT) led to the definition of entities called serovars to distinguish between leptospires on sub-species level, and to the designation of reference strains representing serovars. For decades CAAT has been used to classify leptospires and now approximately 200 serovars have been recognized. In the last few years, it has become increasingly more clear that the serovar concept is no longer fully satisfactory as it may fail to adequately define epidemiologically important entities. In addition, CAAT is too cumbersome and time-consuming for routine typing. Various methods have been developed based on antigenic or genetic analysis with the purpose to supplement or to replace the CAAT. Most of these methods are still in an experimental state. It is to be expected that a typing method based on genomic analysis will eventually become most important. Such a new method should have considerable advantages in order to be acceptable for the development of a new classification system replacing the system based on serovars, which is widely accepted and in many respects still satisfactory. From the new methods, analysis of leptospiral DNA fragment length after digestion with restriction enzymes (REA) has been widely used and proven to be useful for typing. Pending the development of new typing methods that have clear advantages and may lead to a new classification system, it is suggested that the classification system based on serovars is maintained and that REA is added to each description of a new serovar.

DNA↗