Postmortem observations on Fischer rats with leukemia and other disorders.
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A quick staining technique is reported which permits a full cytological statement on the seriousness of mammary tumors within 2 minutes intraoperatively. The accuracy of the method is comparable to that of histological examination. Between 1971 and 1974 a cytological rapid examination was carried out for the assessment of the seriousness of a total of 409 mammary tumors intraoperatively. 71 (equals 17.5 per cent) of the 409 tumors were carcinomas. In 70 of the carcinomas, the severity was confirmed histologically. The method can also be performed in small hospitals which have no pathology laboratory available.
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This study demonstrates the practical advantage of using more than one technique in evaluating breast tumors. Thionin stained, frozen sections and H & E stained, paraffin-embedded, permanent sections yielded a high degree of accuracy in differentiating benign from malignant lesions. Papanicolaou stain is essential for evaluating cytologic material. Special stains, enzyme histochemistry, and examination by SEM and transmission microscopy are essential in identifying the various cellular components of mammary tumors. On the basis of these techniques, fibroadenomas were defined as tumors of stromal cells of the lobule and sclerosing adenosis as a benign proliferation of myoepithelial cells; and it was suggested that mammary cancers may arise from myoepithelial ductal epithelial, or ductular epithelial cells. The behavior of the tumor is related to its cell of origin.
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