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Heart rate variation after breath hold diving with different underwater swimming velocities.

BACKGROUND: The aim of this study was to demonstrate the kinetics of heart rate and blood lactate level obtained after repeated short breath holds with muscular effort in a swimming pool. METHODS EXPERIMENTAL DESIGN: each subject had to perform a series of breath hold diving at rest and three series for different underwater swimming velocities. A series corresponded to six dives of a 30 sec duration separated by a recovery period of 30 sec. Heart rates and blood lactate levels were measured at rest and at the end of each series of breath holds. PARTICIPANTS: the population was composed of 10 male subjects divided into one trained group (5 experts) and one group of 5 beginners. RESULTS: Results indicated a higher bradycardia for the expert group at static breath hold (54.25 vs 65.5 beats x min-1). At the end of a series of breath holds, tachycardia was higher for beginners at different underwater swimming velocities. These values were less significant than the heart rate measured in laboratory despite trials that were abandoned due to high blood lactate levels above 3.5 mmol x l-1. In order to avoid the breath hold breaking point, the maximum heart rate had to correspond to the heart rate of the ventilatory threshold measured in the laboratory, minus the variations of bradycardia measured at rest. CONCLUSIONS: For the training coach, bradycardia was determined by water immersion during a static breath hold. This permitted an evaluation of the level of diver training. A maximal heart rate was attempted to avoid the breath hold breaking point. The results of this study may be useful in creating an effective diver training program.

Adaptation, Physiological↗

Aneuploidy and chromosome breakage in swim-up versus unprocessed semen from twenty healthy men.

Toxicological and epidemiological studies have investigated several factors that are believed to induce cytogenetic damage in human sperm cells in an effort to estimate heritable risk to future generations. Most of these studies have not differentiated damage based on cell fertility or motility. In the clinical setting, intracytoplasmic sperm injection (ICSI) bypasses the natural process of sperm selection. Although practitioners attempt to select motile sperm for ICSI, the sperm may not always demonstrate motility, maturity, or even viability. Knowing whether cytogenetic damage differs in motile versus unselected sperm would improve our ability to estimate heritable risk and lead to improved ICSI procedures, and would expand the body of toxicology and epidemiology research. We divided semen samples from 20 healthy donors and compared aneuploidy and chromosome breakage in sperm cells gathered directly from the ejaculate (unprocessed semen) with cells enriched for motility using the swim-up assay. Sperm fluorescence in situ hybridization was used to detect aneuploidy for chromosomes 13, 18, 21, X, and Y. Tandem labeling probes were used to detect breakage in the 1cen-1q12 region of chromosome 1. The occurrence of disomy 18-18 and XY18 was significantly lower in specimens enriched for motility (P = .004 and P = .001, respectively). Sperm that carried duplication errors and diploid sperm were also seen less frequently in semen analyzed by the swim-up assay (P < .008). Chromosome 1 breakage did not differ between swim up-assayed and unprocessed specimens. Findings suggest that unprocessed semen may overestimate heritable aneuploidy risk in sperm biomarker studies, and may be biologically relevant to ICSI in disomy categories 18-18 and XY18, demonstrating 1.4-fold to 1.8-fold differences.

Adult↗

Effects of azadirachtin on mortality, fertilization, and swimming speed in larvae of the cane toad, Bufo marinus (Anura: Bufonidae).

Experiments were conducted to assess the effects of azadirachtin (AZ) on survival capacity, fertilization success, and swimming speed in larval stages of the cane toad, Bufo marinus . LC50 values (96-hr) for various larval stages exposed to 0.50 mg/l AZ were as follows: 5.35 (stage 12), 5.77 (stage 16), 787 (stage 20), 13.43 (stage 24), and 21.54 (stage 30). Tadpoles did not exhibit any apparent disorientation or production of mucous in response to AZ concentrations ranging from 0.3 to 0.5 mg/l. Mortality was significantly higher at 0.50 mg/l AZ than at lower concentrations. At 0.50 mg/l, mortality rate decreased significantly from 36% for stage 12 tadpoles, to 24% for stage 20 tadpoles. Mortality for early stages (stage 12) dropped significantly, from 36% at 0.50 mg/l, to 21% at 0.40 mg/l AZ There were no significant differences in percent mortality for any developmental stage at an AZ concentration of 0.3 mg/l, or for controls (no AZ). Exposure of gametes to 0.30 mg/l AZ did not reduce fertilization success as compared to controls (89.8 vs. 83.7%). At 0.40 mg/l, percent fertilization decreased significantly (68.8%). This effect was even more pronounced at 0.50 mg/l. No significant difference was found in swimming speeds between controls and subjects exposed to 0.30 mg/l AZ. However, at a concentration of 0.40 mg/l, tadpoles required a significantly longer period of time to swim a distance of 140 cm as compared to controls. The potential impact of AZ on amphibian populations is discussed.

Age Factors↗

[Research on dynamic and synchronous changes of microcirculatory ultrastructure and free radical in rat cerebrum after exhaustive swimming].

This research is designed to detect the dynamic changes of microcirculatory ultrastructure and the MDA content, SOD and GSH-px activity in rat cerebrum immediately, 24h and 48h after exhaustive swimming. The results showed that there were obvious changes of ultrastructure in rat cerebral microcirculation immediately after exhaustive swimming. The changes were resumed in some degree after 24h and recovered almost to the normal after 48h. Immediately after exhaustive swimming, the MDA content did not change obviously; the activity of SOD increased markedly, and then it decreased markedly after 24h, but it increased again after 48h. The activity of GSH-px descended markedly after 24h; it descended synchronously with the decrease of SOD activity, showing that the consumption of SOD and GSH-px should be the most at this time. Therefore, the free radical should be removed in time for reducing the cerebral injury and promoting the recovery of cerebral microcirculation.

Animals↗

[Beneficial effect of swimming in thermal waters on muscle glycogen depletion].

The effect of swimming in the termal water on muscle glycogen stores was studied. After 30 min the muscle glycogen results in a diminution, but it is not depleted. On the contrary, 30 min of swimming in normal water results in a depletion of muscle glycogene stores. The glycemic homeostasis is well maintained in thermal water, and hypoglicemia occurs only after swimming in normal water.

Animals↗

Changes in sperm motility, morphology and concentration induced by the swim-up procedure.

Fifteen semen samples from normal donors were washed twice in Earle's medium by centrifugation and allowed to swim up into overlayered Earle's medium. Aliquots of 0.2 ml were drawn before and after the swim-up, for assessment of sperm motility, morphology and concentration. The percentage of sperm showing progressive motility improved by an average of 54.02 +/- 45.97%, and sperm with the normal morphology increased by 63.8 +/- 31.01%. Head and tail abnormalities decreased by 47.17 +/- 21.3% and 89.37 +/- 16.1% respectively. There was no statistical change in acrosomal abnormalities. Sperm concentration was reduced by 89.02 +/- 7.4%. It is concluded that the standard swim-up technique resulted in an overall improvement in sperm quality, but that the technique was not effective in reducing the acrosomal abnormalities.

Humans↗

Influence of the swim-up procedure on acrosome damage induced by freezing human semen.

Twenty frozen semen samples from normal donors were thawed, washed in Earle's medium, and allowed to swim up using standard techniques. The samples were evaluated before and after the swim up for progressive motility and concentration. Morphologically normal sperm were assessed for acrosomal integrity according to the relative percentages of normal, damaged or lost acrosomes. The motility improved by 75.07 +/- 90.38%, the count was reduced by 97.1 +/- 1.9%, but there was no significant change in the frequencies of the different types of acrosome state. It was concluded that although the swim-up technique is successful in reducing most major morphological abnormalities, influence over the frequencies of secondary acrosomal abnormalities could not be demonstrated.

Acrosome↗

Composition of lipid foams from swim bladders of two deep ocean fish species.

Lipid-containing deposits within the swim bladders of Coryphaenoides acrolepis and Antimora rostrata were investigated. Lipid analysis of this material, which was quite uniform from the two species, yielded the following data: neutral lipids, 36.0-41.7%; phospholipids, 53.6-56.7%; and glycolipids, 4.3-8.9%. Cholesterol (mainly in the free form) constituted 60.4%-77.8% of the neutral lipids. Sphingomyelin and phosphatidylcholine were the principal phospholipids, with sphingomyelin highest in the material from C. acrolepis and phosphatidylcholine predominant in that from A. rostrata. The overall pattern of lipids shows a resemblance to that of plasma membrane, particularly in the relatively high levels of free cholesterol, sphingomyelin, and phosphatidylserine. The lipid-to-protein ratio of the material is approximately 1.5-2 to 1. The lipids of the fine inner lining (tunica interna) of the swim bladder from a shallow water fish, the kelp bass (Paralabrax clathratus), had essentially the same composition as the much more abundant swim bladder material from the deep ocean fishes.

Air Sacs↗

[Use of the swimming test for demonstrating antidepressive activity of drugs during single and repeated administration].

After placing rats or mice into the cylinders filled with water the animals after initial period active swimming, take the immobilization position the time of which is minimized by administering antidepressants. Experiments were made with random-bred, tetrahybrids CBWA, and C57BL/6, BALB/c, CBA, F1 CBA/c55BL mice. Tetrahybrids CBWA appeared to be an optimal species for making experiments. The use of the "swimming test" made it possible to identify the activity of tricyclic (desipramine, chlorimipramine, amitryptyline) and atypical antidepressants (befuralin, zimelidine, trazodon), that of pyrazidol (type A MAO inhibitor) and of a number of new compounds--derivatives of benzofuran and morpholine upon single and chronic administration. To define the method specificity, use was made of the neuroleptic haloperidol, the tranquilizer diazepam, and of nembutal, which did not exhibit any activity in the test in question. Psychostimulants (amphetamine, caffeine) dramatically increased the time of active swimming. The effect lasted throughout all the 30 minutes of testing, which is not characteristic for antidepressants.

Administration, Oral↗

Descriptive statistics of swimming behavior of Schistosoma mansoni miracidia in artificial pond water.

Timed-exposure, dark-field photography was used to characterize the swimming behavior of Schistosoma mansoni miracidia in artificial pond water. Histograms and descriptive statistics were determined for helical swimming amplitude and wavelength, linear speed, turning frequency, and turning angle. Turning frequency could not be distinguished from a Poisson distribution so that turning appeared to occur randomly. Amplitude and wavelength showed no correlation. Faster miracidia exhibited less helical pattern while swimming. The effects of vertical movement of miracidia and of miracidial concentration were determined to be minimal.

Animals↗

31P-NMR study on effects of electric shock on swimming leeches, Hirudo medicinalis.

1. We evaluated the effect of electric shock on swimming leeches by measuring changes in high-energy phosphate metabolism using in vivo 31P-NMR. 2. Leeches electrically stimulated during swimming showed anodal galvanotaxis and stopped swimming with stimulation at strong current. 3. Comparison of the concentrations of high-energy phosphate metabolites before and after electric shock using 31P-NMR revealed a marked decrease in beta-ATP and an increase in that of Pi. 4. Electric shock apparently induces excessive muscle fatigue in leeches, resulting in transient paralysis.

Animals↗

Rapid down-regulation of beta-adrenergic receptors evoked by combined forced swimming test and CGP 37849--a competitive antagonist of NMDA receptors.

The influence of CGP 37849, a competitive antagonist of NMDA, receptors and desipramine (DMI) on the density of beta-adrenergic receptors in rats exposed to the forced swimming test was investigated. CGP 37849 (10 mg/kg) and DMI (15 mg/kg), given twice to rats (24 h apart), or the forced swimming test alone failed to alter the density of beta-adrenergic receptors in the rat cortex, as tested using [3H]dihydroalprenolol ([3H]DHA). However, in rats injected with CGP 37849 (10 mg/kg) or DMI (15 mg/kg) and exposed to the forced swimming test, it was found that the density of beta-adrenergic receptors in the cortex was decreased. It was also observed that CGP 37849 used in a dose which decreased the density of beta-adrenergic receptors reduced the immobility time in a manner similar to DMI. It is concluded that CGP 37849 may evoke similar adaptive receptor changes as anti-depressant drugs which-in turn-suggests antidepressant-like properties of CGP 37849.

2-Amino-5-phosphonovalerate↗

Compromised sperm protein phosphorylation after capacitation, swim-up, and zona pellucida exposure in teratospermic domestic cats.

Tyrosine phosphorylated proteins recently have been found in mouse and human spermatozoa. Our objectives were to (1) determine if domestic cat spermatozoa also express tyrosine phosphorylated proteins, and (2) examine the changes in protein phosphorylation between normospermic and teratospermic domestic cats following sperm capacitation, swim-up separation and exposure to zona pellucida (ZP). Membranes from cat spermatozoa contained two phosphorylated proteins of molecular weights 160 kDa and 95 kDa (designated as p160 and p95) that immunoreacted with monoclonal antibodies to tyrosine phosphate. The p95 protein was distinct from sperm-specific hexokinase. Following capacitation, the extent of phosphorylation of p95 was increased (P < 0.05) 3-fold in normospermic cats compared to only 1.75-fold in teratospermic cats. Similarly, phosphorylation of p160 also increased (P < 0.05) 2.4-fold in normospermic compared to 1.84-fold in teratospermic cats. Although swim-up separation increased the percentage of normal spermatozoa in teratospermic ejaculates, phosphorylation of p95 in swim-up, aliquots was increased (P < 0.05) only 1.95-fold in teratospermic cats compared to 2.9-fold in normospermic counterparts. Likewise, phosphorylation of p160 was lower (P < 0.05) in teratospermic (1.5-fold) compared to normospermic cats (2.0-fold) cats. Phosphorylation also was influenced by exposure to cat ZP proteins (P < 0.05). Solubilized cat ZP bound to the sperm proteins of apparent molecular mass 120, 95, 50, 42, 30, 27, 23 and 20 kDa, suggesting a direct binding interaction between p95 and the ZP. Overall, these findings (1) indicate the presence of tyrosine phosphorylated proteins in the domestic cat spermatozoon that directly interact with homologous ZP glycoproteins; (2) demonstrate that cat sperm hexokinase is not phosphorylated on tyrosine residues; and (3) suggest that the diminished phosphorylation efficiency of sperm from teratospermic cats may result in a compromise in capacitation and the acrosome reaction.

Animals↗

Swimming pool drownings and near-drownings among California preschoolers.

OBJECTIVE: To describe a significant but poorly understood public health problem, the authors compiled data on swimming pool drownings and near-drownings requiring hospitalization for California children ages 1 to 4. METHODS: Data from death certificates were used to analyze swimming pool drownings, and hospital discharge data were used to analyze near-drownings. RESULTS: Among California preschoolers in 1993, pool immersion incidents were the leading cause of injury death and the eighth leading cause of injuries leading to hospitalization. Rates per 100,000 population were 3.2 for fatalities and 11.2 for nonfatal incidents, with a fatality-to-case ratio of 1:3.5. Total charges for initial hospital stays (excluding physicians' fees) were $5.2 million for 1227 hospital days. CONCLUSIONS: Swimming pools remain a serious hazard for young children. Primary prevention continues to be an important public health goal. Public health officials should support the adoption of laws designed to protect children from drowning and near-drownings.

Age Distribution↗

Plasma chloroform concentrations in swimmers using indoor swimming pools.

This study evaluated swimmers and visitors who were exposed to chloroform (CHCl3) at three indoor swimming pools in Modena, Italy. Chloroform was measured in plasma samples of 127 subjects present at the pools and in 40 nonexposed subjects. The analyses were performed by head-space gas chromatography. Chloroform was present in all samples collected from the 127 subjects who attended the pools (median = 7.5 nmol/l; range = 0.8-25.1 nmol/l). Agonistic swimmers who trained for competitions showed a significantly higher mean value of plasma CHCl3 than nonagonistic swimmers and visitors. Plasma CHCl3 levels were significantly correlated with (a) CHCl3 concentrations in water and in environmental air, (b) the number of swimmers in the pools, and (c) the time spent swimming. Covariance analysis showed that plasma CHCl3 levels also depended on the intensity of the sport activity (total explained variance = 67.4%).

Air Pollutants↗

Summary of 58 cases of loss of consciousness during underwater swimming and diving.

It is well accepted that hyperventilation before breath hold swimming and diving makes it possible for a person to extend the time under water. Less well known is the fact that this maneuver can cause loss of consciousness due to hypoxia. This accident happens almost exclusively to males (56 cases). The most common age group was 16-20 years (range 12-33 years). All were known to be good swimmers or divers. Approximately 80% of the cases occurred in guarded pools. Thirty-five subjects survived the accident and of the twenty-three fatalities, there was only one good autopsy report. In this instance the findings were those associated with classical drowning preceded by hypoxia and hypercapnia. Breath holding experiments indicated that the times between loss of consciousness and death may be no longer than 2.5 minutes. The patterns associated with these cases suggest that those who are responsible for aquatic safety as supervisors or guards of pools could prevent most accidents by watching for young male swimmers who are practicing hyperventilation and underwater swimming in competition with themselves or with others.

Adolescent↗

Some thoughts on swimming horses in a pool.

Several indications for swimming horses are recalled. A satisfactory pool and the technique for its use are described. Some observations on the effect on swimming are offered.

Animals↗

Motoneurons of the axial swimming muscles in hatchling Xenopus tadpoles: features, distribution, and central synapses.

Xenopus tadpole motoneurons make cholinergic synapses within the spinal cord. This excitation changes with longitudinal position and contributes to the excitation that controls motor activity and its longitudinal spread during swimming. To explore the anatomic constraints on this excitation, backfilling has been used to examine the anatomy and distribution of the whole population of spinal motoneurons, to define the extent of their central axons and to find where they make synapses. Motoneuron features show considerable variation but do not allow their separation into primary and secondary. Most motoneurons have descending central axons and it is likely that central synapses are made from these axons as longitudinal dendritic extent is very limited. Motoneuron density reaches a broad plateau over the mid-trunk region at 12-13 per 100 microm. Soma size does not change with longitudinal position, but the dorsoventral extent of the dendrites decreases caudally, whereas the central axon length increases. Motoneuron distribution data were used to estimate the longitudinal distribution of central motoneuron axons. This has a broad plateau at 12-14 per 100 microm over much of the trunk and only decreases significantly caudal to the anus. This distribution correlates with cholinergic excitation during swimming. Transmission electron microscopy of motoneurons backfilled with horseradish peroxidase was used to show that central motoneuron axons make en passant synapses with motoneuron dendrites and the dendrites of other unstained neurons. By using measures of synapse frequency and total dendrite length, trunk motoneurons are estimated to each receive 100-200 synapses.

Acetylcholine↗