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An in vitro study of the pH of three calcium hydroxide dressing materials.

Calcium hydroxide is widely used as a root canal dressing material because of its favorable alkalinizing effect. It has been suggested that the action comes from diffusion of hydroxyl ions through the apical foramen. The purpose of this in vitro study was to test the pH changes that occurred over a period of 30 days using a mixture of calcium hydroxide and distilled water and two commercial calcium hydroxide products in a simulated periapical environment. The materials were inserted in glass tubes closed at one end, which were placed in individual vials containing distilled water at a pH 7.4. Unfilled glass tubes were used as controls. Alkalinity changes of the medium were measured at 1 and 24 h and 15 and 30 days. The alkalinizing properties of all materials showed a rapid increase at 1 and 24 h followed by a continuous but more gradual increase from 15 to 30 days. The control tubes did not cause a change in pH of the medium, which remained at pH 7.4. At the end of the observation period, the alkalinizing properties of Calasept and Ultracal XS were significantly higher (P < 0.05) than the calcium hydroxide/distilled water paste.

Analysis of Variance↗

Silver-containing alginate fibres and dressings.

Silver is a broad-spectrum antimicrobial agent that is gaining more and more importance in the wound-management industry. By incorporating silver ions into alginate fibres, highly absorbent alginate wound dressings with antimicrobial properties can be made. Laboratory studies have shown that by incorporating fine particles of silver sodium hydrogen zirconium phosphate, the silver-containing alginate fibres can maintain the white physical appearances while providing a sustained release of silver ions when in contact with wound exudates. Test results have proven that these fibres are highly effective against bacteria.

Alginates↗

Microbiological evaluation of dressing procedures for crocodile carcases in Queensland.

Microbiological testing of crocodiles during the dressing procedure has shown that sanitising the skin before skinning reduces the bacterial count on the skin and that dipping crocodile meat in 1.3% acetic acid solution effectively reduces bacterial levels. The total bacterial count on the processed mean sample was comparable with that obtained in the beef, pork and lamb industries. Salmonellae were isolated from 14 of the 72 carcases. Most (65%) of these isolates were in Salmonella subspecies III, formerly classified as Arizona.

Abattoirs↗

Bioassay for nisin in milk, processed cheese, salad dressings, canned tomatoes, and liquid egg products.

A sensitive nisin quantification bioassay was constructed, based on Lactococcus lactis chromosomally encoding the nisin regulatory proteins NisK and NisR and a plasmid with a green fluorescent protein (GFP) variant gfp(uv) gene under the control of the nisin-inducible nisA promoter. This strain, LAC275, was capable of transducing the signal from extracellular nisin into measurable GFPuv fluorescence through the NisRK signal transduction system. The LAC275 cells detected nisin concentrations of 10 pg/ml in culture supernatant, 0.2 ng/ml in milk, 3.6 ng/g in processed cheese, 1 ng/g in salad dressings and crushed, canned tomatoes, and 2 ng/g in liquid egg. This method was up to 1,000 times more sensitive than a previously described GFP-based nisin bioassay. This new assay made it possible to detect significantly smaller amounts of nisin than the presently most sensitive published nisin bioassay based on nisin-induced bioluminescence. The major advantage of this sensitivity was that foods could be extensively diluted prior to the assay, avoiding potential inhibitory and interfering substances present in most food products.

Animals↗

INCIDENCE OF SALMONELLAE IN DRESSED BROILER-FRYER CHICKENS.

Salmonellae were isolated from 72 of 264 broiler-fryer type chickens that had been purchased in retail stores in the Lafayette, Ind., area in 1963. Meat from the tail area and giblet portions were used in sampling. Equal numbers of dressed whole and cut-up birds were positive for salmonellae. Thirteen different serotypes were identified, the more common being Salmonella infantis, S. reading, and S. blockley. Incubation at 43 C of the blended sample in Selenite-F Enrichment broth containing cystine gave a larger number of recoveries than did incubation at 37 C. There was no significant difference between the means for the birds that yielded salmonellae and those that did not in the locally processed group, when compared for numbers of aerobic microorganisms at 37 C, coliforms, or most probable number of enterococci. In a comparison of poultry processed in-state by the five processors included in the study with that processed out-of-state, there was a general trend for a larger number of positive specimens in the locally produced group. The fall season was an exception.

Animals↗

Microbiological spoilage of mayonnaise and salad dressings.

Saccharomyces bailii was isolated from two-thirds of the spoiled mayonnaise and salad dressing samples examined. Most of the rest were spoiled by Lactobacillus fructivorans. However, one sample contained large numbers of both S. bailii and L. plantarum. Two of the spoiled samples also contained small numbers of bacilli. Bacillus subtilis, B. pumilis, B. polymyxa, B. megaterium, and B. licheniformis were found in one sample and B. subtilis and B. pumilis in another. Small numbers of B. subtilis and B. licheniformis were also present in one unspoiled sample. Several media were evaluated for the isolation of L. fructivorans. S. bailii and L. fructivorans vigorously fermented glucose. The concentration of glucose in the spoiled samples ranged from 0 to 38.5 g/kg and from 1.3 to 17.8 g/kg for the unspoiled samples.

Journal Article↗

R-factors of Escherichia coli from dressed beef and humans.

One hundred eighty Escherichia coli strains isolated from raw and cooked dressed beef and from healthy humans were screened for resistance to each of nine antibiotics: chlortetracycline, ampicillin, chloramphenicol, kanamycin, neomycin, nalidixic acid, dihydrostreptomycin, oxytetracycline, and tetracycline. Nearly 80% of the 98 beef isolates and 54% of the 82 human isolates were resistant to one or more of the antibiotics tested. Ampicillin resistance was most frequent among beef isolates, and dihydrostreptomycin resistance was most frequent among isolates of human origin. About 74% of the multiply resistant beef strains and 85% of the multiply resistant human strains transferred all or part of their resistance to E. coli K-12 recipients.

Ampicillin↗

Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups.

For all adeno-associated virus (AAV) serotypes, 60 monomers of the Vp1, Vp2, and Vp3 structural proteins assemble via an unknown mechanism to form an intact capsid. In an effort to better understand the properties of the capsid monomers and their role in viral entry and infection, we evaluated whether monomers from distinct serotypes can be mixed to form infectious particles with unique phenotypes. This transcapsidation approach consisted of the transfection of pairwise combinations of AAV serotype 1 to 5 helper plasmids to produce mosaic capsid recombinant AAV (rAAV). All ratios (19:1, 3:1, 1:1, 1:3, and 1:19) of these mixtures were able to replicate the green fluorescent protein transgene and to produce capsid proteins. A high-titer rAAV was obtained with mixtures that included either serotype 1, 2, or 3, whereas an rAAV of intermediate titer was obtained from serotype 5 mixtures. Only mixtures containing the AAV4 capsid exhibited reduced packaging capacity. The binding profiles of the mixed-virus preparations to either heparin sulfate (HS) or mucin agarose revealed that only AAV3-AAV5 mixtures at the 3:1 ratio exhibited duality in binding. All other mixtures displayed either an abrupt shift or a gradual alteration in the binding profile to the respective ligand upon increase of a capsid component that conferred either HS or mucin binding. The transduction of cell lines was used to further evaluate the phenotypes of these transcapsidated virions. Three transduction profiles were observed: (i) small to no change regardless of ratio, (ii) a gradual increase in transduction consistent with titration of a second capsid component, or (iii) an abrupt increase in transduction (threshold effect) dependent on the specific ratios used. Interestingly, an unexpected synergistic effect in transduction was observed when AAV1 helper constructs were combined with type 2 or type 3 recipient helpers. Further studies determined that at least two components contributed to this observed synergy: (i) heparin-mediated binding from AAV2 and (ii) an unidentified enhancement activity from AAV1 structural proteins. Using this procedure of mixing different AAV helper plasmids to generate "cross-dressed" AAV virions, we propose an additional means of classifying new AAV serotypes into subgroups based on functional approaches to analyze AAV capsid assembly, receptor-mediated binding, and virus trafficking. Exploitation of this approach in generating custom-designed AAV vectors should be of significant value to the field of gene therapy.

Animals↗

Plasticized polyvinylchloride as a temporary dressing for burns.

Plasticized polyvinylchloride film has been used in this burns unit for a long time for dressings before the ward round, before surgery, and when the burned patient is transferred from the casualty department to the burns unit. Plasticized polyvinylchloride film is easy to use, safe, and causes no pain. Most importantly, in the present financial climate, it is cheap.

Bandages↗

Surgical instruments and dressings: an information service.

A regional information service on surgical equipment, instruments, and dressings is proposed that would be staffed by information scientists. It would appreciably reduce the time spent on administration and make the buying of equipment more efficient. Such a service could be extended to all health service staff.

Bandages↗

Factors concerned in the efficient steam sterilization of surgical dressings.

Some of the factors affecting the efficient steam sterilization of dressings have been examined. A jacketed sterilizer will process a load more quickly than a sterilizer without a jacket. The level of fore-vacuum is critical and must reach an absolute pressure of 20 mm. Hg (29.2 in.Hg vacuum) or less. This will overcome all conditions of overpacking studied. The level of after-vacuum should be 100 mm. Hg absolute or less, preferably near 50 mm. Hg absolute. Overpacking cannot be defined in terms of weight of a fabric per unit volume of container but occurs when a load is compressed in its container.

Bandages↗

Our method of povidone-iodine ointment and gauze dressings reduced catheter-related infection in serious cases.

In experiment 1, we evaluated our method of catheter care at subclavian vein insertion sites for the control of catheter-related infections in seriously ill neurosurgical patients who needed prolonged catheter placement, compared with an older method. In our method, the insertion site was prepared with 10% povidone-iodine solution, followed by application of 10% povidone-iodine ointment, and covered with sterile gauze and a transparent polyurethane dressing. The older method was based on 1996 guidelines for the prevention of intravascular device-related infections. Catheter colonization and mortality were both found to be significantly reduced with our method (p = 0.0214, p = 0.0379, respectively). In experiment 2, we evaluated whether a regimen of catheter care with 10% povidone-iodine ointment was more effective than that without povidone-iodine ointment for the prevention of infections. This suggested effectiveness of 10% povidone-iodine ointment for reduction of infection. Our method of catheter care was useful even in seriously ill neurosurgical patients.

Administration, Topical↗

Is pressure dressing necessary after ear surgery?

Compressive bandages carry intrinsic risks and complications. The purpose of this study was to assess whether compressive head bandages are necessary after ear surgery to prevent complications such as hematoma or wound infection. A prospective, randomized, controlled study was conducted from August 1, 1993, to June 1, 1999. We randomly assigned 420 consecutive middle ear or mastoid operations to either a pressure bandage group or to a no-pressure bandage group. A careful follow-up was planned, and complications were recorded. In the pressure bandage group, 3 patients had bruising over the pinna and 70 patients had minor skin erythema when the dressing was removed. No patient had bruising or erythema in the no-pressure bandage group. No patient had hematoma, wound infection, or any other wound complication in either group. As a result of this study, we have decided to abandon the use of compression bandages after uncomplicated ear surgery.

Adolescent↗

Use of op site as an occlusive dressing for total parenteral nutrition catheters.

A retrospective study of standard hyperalimentation catheter dressing compared to the use of Op Site has demonstrated that Op Site is cost and time effective and is efficacious for attaining a low catheter sepsis rate. It is easy for nursing personnel to apply and comfortable for the patients to wear. Op Site may be contraindicated in diaphoretic patients.

Adolescent↗

Dressing changes by specialized personnel reduce infection rates in patients receiving central venous parenteral nutrition.

In 60 pediatric patients, 75 central venous catheters representing 1866 patient days were placed to provide parenteral nutrition. During the 21-month study period, surgical residents were responsible for dressing changes during the first 16 months and a specially trained nurse was responsible during the final 5 months. Twenty percent of the lines became infected. The infection rate was significantly higher in the lines cared for by residents, 28.8% compared to 3.3% in the lines cared for by the nurse. This occurred in spite of the lines being in place significantly longer in the nurse group (31.7 vs 20.3 patient days/line). These data indicate that a specially trained person using aseptic techniques can reduce infection rates in patients receiving central venous parenteral nutrition.

Bacterial Infections↗

A cotton batting compression dressing and fiberglass cast used safely in the immediate postoperative period after hindfoot or ankle surgery.

BACKGROUND: Patients undergoing surgery on the foot and ankle who require immobilization are typically placed in splints or soft compression dressings after surgery to avoid complications associated with swelling. As swelling subsides, this temporary immobilization is removed and replaced with a short-leg cast, making an additional office visit necessary, which adds to the cost as well as patient discomfort. METHODS: We apply a cast in the operating room using a special padding technique to accommodate postoperative swelling. It is supportive enough that it does not need to be replaced shortly after surgery. This cast was applied to 38 patients having surgery on the hindfoot or ankle between March, 2003, and February, 2004. RESULTS: All patients tolerated the cast during the initial 2 weeks after surgery, and there were no complications. CONCLUSION: The purpose of this study was to describe the casting technique and document its safety for use immediately after surgery on the hindfoot and ankle.

Adolescent↗

Transparent film for intravascular catheter exit-site dressings.

Transparent polyurethane film is recommended for catheter site insertion dressing by the American guidelines for the prevention of infections associated with intravascular catheters. It has been proven to offer the advantages of excellent adhesion, firm support of the catheter, good tolerability, ease of application and fewer replacements per catheter lifetime. The last two features save nursing time and result in healthcare cost savings.

Journal Article↗

Patient-controlled analgesia with fentanyl for burn dressing changes.

In this randomized, double-blinded study in 60 ASA I or II adults with >20% body-surface area thermal burns, we investigated the feasibility of patient-controlled analgesia (PCA) with fentanyl for pain management during dressing changes and determined the optimal PCA-fentanyl demand dose. An initial loading dose of IV fentanyl 1 microg/kg was administered. Patients received on-demand analgesia with fentanyl (10, 20, 30, and 40 microg) whenever their visual analog scale (VAS) score was >2. Mean VAS scores in the 10 and 20 microg groups (7.73 +/- 1.33 and 7.20 +/- 1.21, respectively) were significantly higher than those in the 30 and 40 microg groups (4.47 +/- 0.83 and 3.90 +/- 0.63, respectively) (all P = 0.000). Demand/delivery ratios were significantly larger in the 10 and 20 microg groups (3.03 +/- 1.06 and 2.54 +/- 0.49, respectively) than those in the 30 and 40 microg groups (1.36 +/- 0.34 and 1.37 +/- 0.36, respectively) (all P = 0.000). VAS scores and demand/delivery ratios were comparable in the 30 and 40 microg groups (P = 0.260 and P = 0.977, respectively), which suggests comparable analgesic efficacy. There was no hemodynamic instability or respiratory depression. The optimal demand dose of PCA-fentanyl was 30 microg (5-min lockout interval) after an initial loading dose of IV fentanyl 1 microg/kg.

Adult↗