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Responses of Arctic and tropical men to a standard cold test and peripheral vascular responses to local cold stress in the Arctic.

Thermoregulatory, metabolic and peripheral vascular responses to cold were studied in two groups (six each) of healthy men during exposure to the natural cold environment of the Arctic. Group A comprised of two arctic natives and four temporary residents who had migrated from the temperature zone of Russia. Group B consisted of six soldiers from a tropical region (India). Group B was airlifted to the arctic (70 degrees N, 38 degrees E). Both groups stayed in a field camp. The experiments were conducted during the 7th week of the stay. The volunteers were subjected to a standard cold test at 10 degrees C for 2 h wearing only shorts. Their heart rate, blood pressure, ventilation, oxygen consumption, oral temperature, mean skin and extremity temperatures were recorded initially and at 30 min intervals during standard cold test. The cold-induced vasodilatation response in both groups was also studied separately. The tropical natives (Group B) were flown back to Delhi and retested after 6 weeks. The physiological responses to general cold exposure as well as peripheral vascular response to local arctic cold stress were similar in both groups. The observation suggested that cold acclimatization in tropical men is similar to that of the people of Russian origin from a temperature zone.

Acclimatization↗

[In memory of Paul Bert (1833-1886) and the development of high altitude physiology in Switzerland].

It is the merit of Paul Bert to have assigned as an important task to experimental physiology the investigation of mountain sickness, of immediate accommodation of the organism to high altitude, and of its long-term acclimatation. In his "Pression Barométrique" of 1878, he established the foundations of high-altitude physiology. In particular, he formulated the "law of partial pressure", the partial pressure of the atmospheric gases alone being responsible for their action on the organism. Paul Bert's historic role, for a long time nearly forgotten, has been recalled to memory by the Swiss medical historians E.H. Ackerknecht and N. Mani. Bert's line of investigation has been followed especially by Angelo Mosso of Turin and, more recently, by F. Verzár, A. von Muralt, A. Fleisch and other Swiss physiologists.

Acclimatization↗

Operation Everest II: gas tensions in expired air and arterial blood at extreme altitude.

Measurements in alveolar gas have suggested extreme hypocapnia and alkalosis on the summit of Mt. Everest. However, tensions in both expired gas and arterial blood have not been reported for the summit of Mt. Everest (PIO2 = 43 mm Hg). To approach the problem, we examined alveolar (and end-tidal) and arterial gas tensions in 6 healthy men who completed a 40-d chamber study to the simulated "summit," with 20 d above 6,400 m and 9 d above 8,000 m. In 27 simultaneous samples of alveolar air and arterial blood for inspired oxygen tensions ranging from PIO2 of 55 mm Hg (7,380 m) to 43 mm Hg, the mean alveolar-arterial difference was negligible for PO2 (-0.8 +/- 2.4 (S.D.) mm Hg) and PCO2 (0.5 +/- 1.4 mm Hg). For all 6 subjects at the summit, PACO2 was 12.0 +/- 1.8 and PACO2 was 11.4 +/- 1.6 mm Hg, and for the two with the lowest values, alveolar and arterial PCO2, respectively, were 9.5 and 9.8 mm Hg. Arterial pH averaged 7.53 units. We conclude that while acclimatization to severe hypoxia results in extreme hypocapnia, alkalosis is more moderate than previously reported. Alveolar gas tensions reflect well the values obtained in arterial blood.

Acclimatization↗

Body weight loss during acute hypoxia: effects of increased convective oxygen transport or previous acclimation.

Body weight loss and growth retardation occur in rats exposed to simulated high altitude, which may be related to the hypoxemia-induced reduction in the convective oxygen transport (COT). The present study was thus performed to determine whether transfusion polycythemia, increased affinity of hemoglobin for oxygen, or previous acclimation to hypobaria (factors that increase COT) are able to counteract its effect on body weight during the early period of exposure, which appears to be a suitable parameter to test the effectiveness of acclimatization. Polycythemia was induced in weanling rats by two ip injections of 2.5 ml/100 g b.wt of packed homologous red cells. The rise in hemoglobin O2 affinity was brought about in adult rats by giving them 0.5 g/dl sodium cyanate in the drinking water for 3 weeks. A lower body weight loss during the early period of exposure to hypobaria was seen in treated rats than in controls. However, body weight loss was still important, which would indicate that compensation was probably not complete. When growing rats were acclimated to simulated altitude, a sudden increase in body weight was observed when they were brought back to ground levels. When animals were taken to altitude again, they lost weight at a rate not significantly different to that found in non-acclimated ones. The results obtained indicate that treatments do not prevent the studied effect of hypoxia and suggest that hypophagia and the resultant initial body weight loss and secondary depression of body growth could be considered as protective mechanisms against the environmental challenge, although further investigation will be necessary to confirm the hypothesis.

Acclimatization↗

Cold-induced vasodilatation and peripheral blood flow under local cold stress in man at altitude.

The cold-induced vasodilatation (CIVD) response was studied on 17 lowlanders (20-30 years) at Delhi, using a water bath maintained at 4 degrees+/-0.2 degreesC. The temperatures were measured at the tip of the index finger, centre of the palm, over a prominent wrist vein, and orally. Then the subjects were air-lifted to an altitude of 3500 m, where the measurements were repeated at weekly intervals for a period of 3 weeks. After this, they were flown back and retested. For comparison, the study at altitude was done on 10 acclimatised lowlanders and on 10 high-altitude natives. The peripheral blood flow under a local cold stress was calculated. Three types of CIVD responses--viz. typical hunting, proportional control, and continuous rewarmin--were observed. At altitude, the pattern was more or less the same but there was a significant reduction in the response. The responses of the natives were more or less identical to those of the lowlanders at sea level, and responses of the acclimatized lowlanders were better than the fresh inductees, but much less than the natives. The changes in peripheral blood flow was in accordance with the CIVD response.

Acclimatization↗

Cognitive performance during short acclimation to severe hypoxia.

BACKGROUND: Exposure to high altitudes requires acclimation or acclimatization, to prevent the negative effects of severe hypoxia. Among several methods, short acclimation with intermittent exposure to severe hypoxia in a hypobaric chamber triggers efficient physiological pre-adaptation mechanisms (11-13). However, we have little knowledge about the cognitive repercussions of such an acclimation protocol. METHODS: Four mountaineers were tested daily in the course of a short acclimation protocol (5 d). After their SaO2 (arterial oxyhemoglogin saturation) were recorded, they carried out a choice reaction time task (Manikin test) twice every day; first at ground level (250 m, control sessions), second at the highest altitude of the day (D1 = 5000 m, D2 = 5500 m, D3 = 6000 m, D4 = 6500 m, D5 = 7000 m). RESULTS: High altitude SaO2 level decreased during the first 3 d, then stabilized around 72-73%. Despite a slight and transient increase at the highest altitude relative to the ground level in D4, the error rate remained low throughout the protocol. Further, response time to the Manikin task did not show significant changes among the days during the acute stage of hypoxia relative to ground level up to 7000 m. CONCLUSIONS: On the whole, it seems that a short acclimation protocol based on intermittent exposure to simulated high altitudes triggered adaptive processes without major impairment in a choice reaction time task during the acute stages of severe hypoxia.

Acclimatization↗

[Hand blood flow at rest and during submaximal exercise in acute and chronic heat stress (author's transl)].

Three groups of subjects, Europeans without any heat acclimation (called EE), Europeans after 3 weeks of acclimatization in India (EI), and Indians in their natural environment (II) were studied during exposure to an ambient temperature of 33 degrees C. Hand blood flow (Q), rectal temperature (Tre), mean cutaneous temperature (Tsk) were simultaneously recorded at rest and during 2 periods of muscular exercise (0.44 and 0.7 Vo2 max) of 35 mn duration. The results showed (1) at rest, Q was very high in EE, quite low in both EI and II; (2) at the onset of exercise, a hand vasoconstriction was observed in all cases; (3) during exercise, there was a progressive increase of Q until 200% maximum above rest values; (4) at the end of exercise, Q was proportional to the intensity of the exercise for each group and inversely proportional to the duration of heat exposure, the highest Q was observed in EE, the lowest in II and an intermediate value for EI close to the latter). These differences in hand blood flow could not be explained by differences in deep and/or superficial temperatures between subjects. Thus, during chronic heat exposure, there is, especially for an exercising man, a progressive modification of heat transport in the body: that is, a reduction of skin perfusion and a greater Tre-Tsk difference which are both adaptative responses. The value of hand blood flow as an estimation of whole superficial circulation is discussed.

Acclimatization↗

Risk factors in high altitude pulmonary oedema.

OBJECTIVE: To identify the risk factors for high altitude pulmonary oedema. DESIGN: A case control study. PLACE AND DURATION OF STUDY: The study was conducted in the Department of Medicine, Military Hospital, Rawalpindi during October 1998 to October 2000. SUBJECTS AND METHODS: One hundred serving army personnel between 20 to 50 years of age were divided in two groups. First group comprised of 50 patients who developed high altitude pulmonary oedema at 4000 meters height and above. Second group consisted of 50 control subjects who accompanied the patients. Detailed history was obtained regarding height of native dwelling, rate of ascent, amount of exertion and preceding respiratory tract infection. The findings of both the groups were compared and statistically analyzed by application of Pearson's chi-square test. Difference was considered significant when p-value was < 0.05. RESULTS: All the subjects were male. Average age of the indexed group was 29.4(+/- 4) years as compared to 27(+/- 5) years in the control group. Six patients (12%) were highland dwellers whereas 44 patients (88%) were inhabitants of lower heights. Average rate of ascent from 4000 meters to 4500 meters of the indexed group was 5(+/- 2) days while in control group it was 10(+/- 3) days. Twenty cases (40%) had history of severe exertion whereas such history was not present in any of the control group subjects. Only 2 patients had signs and symptoms of preceding respiratory tract infection. CONCLUSION: Rapid rate of ascent, extreme physical exertion, low height of native dwelling and preceding respiratory tract infection are important risk factors for the development of high altitude pulmonary oedema. Proper acclimatization with gradual ascent plays important role in preventing this potentially fatal condition.

Adult↗

Cholinergic urticaria. A seasonal disease.

Nine young patients with cholinergic urticaria reported that the occurrence of the disease was restricted to winter. They were examined in a climatic room in winter and summer under three climatic conditions in each season (cold, comfort, and severe heat). All subjects experienced severe phenomena of cholinergic urticaria during the winter season when tested under comfortable climate and under severe heat load. No phenomena were elicited in the summer under any of the three climatic conditions or under cold climatic condition in the winter. This finding led to the assumption that symptoms appear in the sufferers only when exposed to heat or heat-producing exercise while unacclimatized to heat. Artificial heat acclimatization is winter resulted in disappearance of all clinical phenomena of cholinergic urticaria, lasting from a few days to two weeks.

Adult↗

Lung volume, chest size, and hematological variation in low-, medium-, and high-altitude central Asian populations.

To evaluate adaptive responses to high-altitude environment, we examined three groups of healthy adult males from Central Asia: 94 high-altitude (HA) Kirghiz subjects (3,200 m above sea level); 114 middle-altitude (MA) Kazakh subjects (2,100 m), and 90 low-altitude (LA) Kirghiz subjects (900 m). Data on chest size (chest perimeter and chest diameter), lung volume (forced expiratory volume (FEV) and forced expiratory volume in 1 sec (FEV1)), and hematological parameters (hemoglobin, erythrocytes, hematocrit, and SaO(2)) are discussed. The results show that 1) chest shape is less flat in the samples living at higher altitude. In the HA sample, chest perimeter is lower but chest excursion is high. 2) In the highlanders, forced vital capacity (FVC) and FEV1 are no higher than in the other samples, even when corrected for stature and body weight. The negative correlation between FVC-FEV1 and age decreases with increasing altitude. 3) The HA and MA samples have higher values of hemoglobin, erythrocytes, and hematocrit. The HA sample has lower SaO(2) and higher arterial oxygen content than the LA sample. No association between hematocrit and age was detected in the four samples. The results indicate that the high-altitude Kirghiz present features of developmental acclimatization to hypobaric hypoxia which are also strongly influenced by other major high-altitude environmental stresses.

Adaptation, Physiological↗

Higher arterial oxygen saturation during submaximal exercise in Bolivian Aymara compared to European sojourners and Europeans born and raised at high altitude.

Arterial oxygen saturation (SaO(2)) was measured at 3,600-3,850 m by pulse oximetry at rest and during submaximal exercise in three study groups: 1) highland Aymara natives of the Bolivian altiplano (n = 25); 2) lowland European/North American sojourners to the highlands with at least 2 months of acclimatization time to 3,600 m (n = 27); and 3) subjects of European ancestry born and raised at 3,600 m (n = 22). Aymara subjects maintained approximately 1 percentage point higher SaO(2) during submaximal work up to 70% of their maximal work capacity, and showed a smaller rate of decline in SaO(2) with increasing work compared to both European study groups. The higher-exercise SaO(2) of Aymara compared to Europeans born and raised at 3,600 m suggests genetic adaptation. The two European study groups, who differed by exposure to high altitude during their growth and development period, did not show any significant difference in either resting or exercise SaO(2). This suggests that the developmental mode of adaptation is less important than the genetic mode of adaptation in determining exercise SaO(2). A weak correlation was detected (across study groups only) between the residual forced vital capacity (FVC) and the residual SaO(2) measured at the highest level of submaximal work output (P = 0.024, R = 0.26). While firm conclusions based on this correlation are problematic, it is suggested that a part of the higher SaO(2) observed in Aymara natives is due to a larger lung volume and pulmonary diffusion capacity for oxygen. Results from this study are compared to similar studies conducted with Tibetan natives, and are interpreted in light of recent quantitative genetic analyses conducted in both the Andes and Himalayas.

Adaptation, Physiological↗

Morphological alterations induced by cold-shock in Neurospora crassa.

The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphological alterations were not detected. In addition, the protein electrophoretic profile of cultures submitted to cold-shock was different from that of cultures kept at 30 degrees C. After 1 and 2 hours at 4 degrees C at least 5 bands were more intense than at 30 degrees C. The results suggest that cold-shock temporarily increases the synthesis of some proteins probably involved in the acclimatization process at 4 degrees C, and that the effects on morphology are probably due to damage to calcium influx.

Calcium↗

Iris pigmentation and photopic visual acuity: a preliminary study.

Visual acuity under varying conditions of light stress was tested in four human populations. It was found that the density of iris pigmentation had no significant effect on visual acuity under conditions of bright light. While some acclimatization to local light levels was observed, significant population differences in visual acuity were obtained. A hypothesis is advanced at to the adaptive value of varying densities of pigmentation of the iris based on the known heat absorption properties of melanin granules.

Anthropology, Physical↗

Growth characteristics of populations of Tibetan origin in Nepal.

Previous growth studies of highland-dwelling populations in the ecologically diverse areas of Peru and Ethiopia have yielded highly varied results: the retarded growth of the Peruvian sample was attributed to the effects of hypoxia, while the increased height and weight of the highland Ethiopian sample could be traced to better health conditions in the highland village than in the lowland village studied. In an attempt to provide a basis for evaluating studies of growth at high altitude, the present study compared Sherpa children living in the Everest region of Nepal with Tibetan children living in Kathmandu. It was found that: (1) the growth of Sherpa and Tibetan children is considerably retarded compared to other high altitude populations; (2) despite conditions favorable for optimum growth among the Tibetans, their growth resembled that of the Sherpas and (3) increased chest circumference, which seems to reflect a developmental acclimatization to hypoxia among Peruvian high-altitude natives, was not seen among the Sherpas.

Adolescent↗

Increased vital and total lung capacities in Tibetan compared to Han residents of Lhasa (3,658 m).

Larger chest dimensions and lung volumes have been reported for Andean high-altitude natives compared with sea-level residents and implicated in raising lung diffusing capacity. Studies conducted in Nepal suggested that lifelong Himalayan residents did not have enlarged chest dimensions. To determine if high-altitude Himalayans (Tibetans) had larger lung volumes than acclimatized newcomers (Han "Chinese"), we studied 38 Tibetan and 43 Han residents of Lhasa, Tibet Autonomous Region, China (elevation 3,658 m) matched for age, height, weight, and smoking history. The Tibetan compared with the Han subjects had a larger total lung capacity [6.80 +/- 0.19 (mean +/- SEM) vs 6.24 +/- 0.18 l BTPS, P less than 0.05], vital capacity (5.00 +/- 0.08 vs 4.51 +/- 0.10 1 BTPS, P less than 0.05), and tended to have a greater residual volume (1.86 +/- 0.12 vs 1.56 +/- 0.09 1 BTPS, P less than 0.06). Chest circumference was greater in the Tibetan than the Han subjects (85 +/- 1 vs 82 +/- 1 cm, P less than 0.05) and correlated with vital capacity in each group as well as in the two groups combined (r = 0.69, P less than 0.05). Han who had migrated to high altitude as children (less than or equal to 5 years old, n = 6) compared to Han adult migrants (greater than or equal to 18 years old, n = 26) were shorter but had similar lung volumes and capacities when normalized for body size. The Tibetans' vital capacity and total lung capacity in relation to body size were similar to values reported previously for lifelong residents of high altitude in South and North America. Thus, Tibetans, like North and South American high-altitude residents, have larger lung volumes. This may be important for raising lung diffusing capacity and preserving arterial oxygen saturation during exercise.

Adult↗

Geographical distribution of hot flash frequencies: considering climatic influences.

Laboratory studies suggest that hot flashes are triggered by small elevations in core body temperature acting within a reduced thermoneutral zone, i.e., the temperature range in which a woman neither shivers nor sweats. In the present study, it was hypothesized that women in different populations develop climate-specific thermoneutral zones, and ultimately, population-specific frequencies of hot flashes at menopause. Correlations were predicted between hot flash frequencies and latitude, elevation, and annual temperatures. Data on hot flash frequencies were drawn from 54 studies. Pearson correlation analyses and simple linear regressions were applied, first using all studies, and second using a subset of studies that included participants only to age 60 (n = 36). Regressions were repeated with all studies, controlling for method of hot flash assessment. When analyses were restricted to studies that included women up to age 60, average temperature of the coldest month was a significant predictor of hot flash frequency (P < 0.01), explaining 29.2% of the variation in hot flash frequency. In a separate equation, the difference between hottest and coldest temperatures was also a significant predictor (P < 0.01), explaining 26.4% of the variation in hot flash frequency. When regressions used all studies but controlled for method of hot flash assessment, average temperature of the coldest month, difference between hottest and coldest temperatures, and mean annual temperature were significant predictors of hot flash frequency. Women reported fewer hot flashes in warmer temperatures, and more hot flashes with increasing seasonality. These results suggest that acclimatization to coldest temperatures or sensitivity to seasonality may explain part of the population variation in hot flash frequency.

Body Temperature Regulation↗

Pulmonary neuroendocrine cells in species at high altitude.

Ever since pulmonary neuroendocrine cells were first described, a chemoreceptor function has been attributed to them. This hypothesis proposes that the innervated clusters of these cells, which are known to degranulate when the oxygen tension around them is reduced, respond to hypoxia to initiate activity in a reflex arc and ultimately adjust some aspect of pulmonary function. If this were true, one might expect to see changes in the pulmonary neuroendocrine system in species exposed to the unremitting hypoxia at natural high altitude. Whilst evidence from some studies suggests that such changes do occur, others have been unable to demonstrate any effect. To some extent this may be attributable to species variability, but might also reflect whether the organism is genetically adapted or merely acclimatized to life in an oxygen-poor environment.

Adaptation, Physiological↗

Pharmacokinetics and pharmacodynamics of bromocriptine in the rat.

The absorption, distribution, and excretion of bromocriptine were studied following oral and parenteral administration of non-radioactive and 14C-labelled drug in the rat. Total radioactivity was measured in blood, tissues, and excreta by liquid scintillation counting while the parent drug was determined in plasma and selected tissues by radioimmunoassay. The pharmacokinetic observations were compared with the time course of drug-induced hypothermia in cold-room acclimatized rats. The results indicated that oral doses of bromocriptine were rapidly, though incompletely (32-40 per cent), absorbed, but underwent extensive first-pass metabolism, resulting in an absolute bioavailability of only 6 per cent. The bioavailability increased to approximately 22 per cent in rats pretreated with the hepatic microsome inhibitor proadifen, thus suggesting the liver as the principal site of biotransformation. Absorbed bromocriptine showed preferential distribution into the tissues, although no apparent accumulation of drug-related material occurred in the body. The drug was eliminated almost exclusively by metabolism and biliary excretion into the faeces. Comparison of the pharmacodynamic and the pharmacokinetic profiles indicated a dose relationship between hypothermia and plasma bromocriptine concentrations but not total radioactivity levels. The hypothermic response was also intensified by proadifen pretreatment, thus confirming the parent drug as the pharmacologically active entity. It is believed that the previously reported delay in the onset of bromocriptine activity is not pharmacokinetic in nature, but is related to the properties of the receptors at the target site or to the pharmacologic events that result in the observed effects.

Animals↗