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At least 1,693 records · Page 94Linked to original sources
PubMed1995

Human capillary endothelial cells from abdominal wall adipose tissue: isolation using an anti-pecam antibody.

We have developed a novel isolation technique for harvesting human capillary endothelial cells. We compared the use of either Ulex Europaeus Agglutinin (UEA) lectin or anti-platelet endothelial cell adhesion molecule (PECAM) antibody conjugated to magnetic beads for the ability to isolate and maintain pure cultures of human capillary endothelial cells. Cells isolated using either method actively scavenged DiI-acetylated-low density lipoprotein and expressed von Willebrand factor (vWf) up to four passages as assessed by immunofluorescent labeling. Endothelial cells isolated using the anti-PECAM antibody method maintained these endothelial-specific properties for up to 12 passages while the percentage of UEA selected cells expressing these properties decreased during increasing passage number. Furthermore, while both techniques yielded cells that bind UEA at Passage six, only the antibody selected cells expressed the normal pattern of endothelial-specific cellular adhesion molecules as assessed by flow cytometry. Both cell isolates were cultured within a three-dimensional matrix of type I collagen, the antibody selected cells formed tubelike structures within 2 days, while the lectin selected cells did not. The antibody selected capillary endothelial cells were transduced with a retroviral vector containing the human growth hormone cDNA and were found to secrete growth hormone from both two- and three-dimensional cultures. We propose that anti-PECAM antibodies linked to a solid support provide a highly selective step in the isolation and maintenance of pure populations of human capillary endothelial cells from abdominal wall liposuction remnants.

Abdomen↗
PubMed2002

[The use of Foley's catheter and suturing to control abdominal wall haemorrhage provoked by the trochar. Comparative study].

Lesions of the abdominal vessels are becoming more frequent owing to the increase in the number of laparoscopies carried out with trochars: they occur in fact in 2% of cases. A study has been carried out to compare the effectiveness of 2 methods for stopping haemorrhage: the use of a Foley catheter and suturing with a Reverdin needle. Abdominal haemorrhages take place as a result of lesions during introduction of accessory trochars in the superficial, circumflex and deep epigastric vessels. Six patients were treated with Foley's catheter while 13 were treated with Reverdin's needle under visual control, maintaining the same operative route, something that is not possible with the Foley catheter. Patients treated with suturing were discharged within 24 hours without irritation or pain.

Abdominal Wall↗