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Pointing at objects in other rooms: young children's sensitivity to perspective after walking with and without vision.

Locomotion alters the spatial structure of an observer's perspective, that is, the network of observer to environment distances and directions. The purpose of the present 6 experiments was to investigate the sensitivity of 12-48-month-olds to changes in perspective that are occluded from view by walls and by darkness. To assess sensitivity, children were shown a target object in one room, walked into an adjacent room and asked to point in the straight-line direction at the target. In Experiment 1, 42 12-48-month-olds were tested and results indicated that children older than 36 months responded by pointing straight at the occluded target, whereas younger children tended to point in the direction of their route away from the target. In Experiments 2-4, 24- and 48-month-olds were tested and results demonstrated that 48-month-olds were sensitive to the proprioceptive and to the visual-environmental cues for the changes in perspective structure. The 24-month-olds, however, responded by pointing straight toward the target when visual-environmental cues were absent, whereas they pointed in the direction of their route when they were present. In Experiments 5 and 6 additional 24-month-olds were tested to assess the effects of short-term training and of a continuous view of the target on responding in the presence of visual-environmental cues. The results indicated relatively early sensitivity to proprioceptive cues for changes in perspective and somewhat later sensitivity to appropriate visual-environmental cues under these conditions.

Child Development↗

[Relation of visual evoked potentials to the integral characteristics of cognitive activity in 8- to 9-year-old children].

Amplitude and temporal parameters of evoked potentials (EPs) in response to a checkerboard pattern, picture of a house, the Russian word "dom", meaning house, combination of letters "DMO" and a number of other stimuli, correlated with indices of the verbal, nonverbal, and general intelligence as well as with those of the cognitive style "field dependence-independence". Maximum number of statistically significant correlations was found in the course of comparison of integrative cognitive characteristics and parameters of EPs to stimuli with increased semantic complexity. No interhemispheric differences were found in the character of interrelations between cognitive characteristics and parameters of EPs to verbal and spatially structured stimuli.

Child↗

[Conformation states of thymopentin and its synthetic analogs].

Spatial structures of the biologically active fragment Arg-Lys-Asp-Val-Tyr of tymopoetin (tymopentin) and its synthetic analogs: [Lys1-Arg2]-, [Glu3]- and [Gly3] have been investigated by theoretical conformational analysis. These results indicate that the conformational properties of fragments are represented in aqueous solution by five different backbone forms.

Amino Acid Sequence↗

[Clinico-electroencephalographic correlations in asthenoadynamic subdepressions].

In patients with atypical, torpid and poorly identifiable asthenoadynamic subdepressions with predominant disorders of intellectual activity the authors analyzed the spatial structure of the ECG activity. They also studied the psychophysiologic mechanisms underlying the psychopathologic condition. The feasibility of the objective verification of the clinical status and of the prognosis of the depressive condition have been demonstrated. The data on the overactive processes of cortical excitation and deficit of the processes of active selective cortical inhibition in the studied states are presented.

Adolescent↗

[Supramolecular organization of enzymes of the tricarboxylic acid cycle].

In virtue of analysis of data on the interaction of tricarboxylic acid cycle enzymes with the mitochondrial inner membrane and data on the enzyme-enzyme interactions, the spatial structure for the tricarboxylic acid cycle enzyme complex (tricarboxylic acid cycle metabolon) is proposed. The alpha-ketoglutarate dehydrogenase complex, adsorbed on the mitochondrial inner membrane along one of its 3-fold symmetry axes, plays the key role in the formation of metabolon. Two association sites of the alpha-ketoglutarate dehydrogenase complex located on opposite sides of the complex participate in the interaction with the membrane. The tricarboxylic acid cycle enzyme complex contains one molecule of the alpha-ketoglutarate dehydrogenase complex and six molecules of each of the other enzymes of the tricarboxylic acid cycle, as well as aspartate aminotransferase and nucleosidediphosphate kinase. Succinate dehydrogenase, the integral protein of the mitochondrial inner membrane, is a component of the anchor site responsible for the assembly of metabolon on the membrane. The molecular mass of the complex (ignoring succinate dehydrogenase) is of 8.10(6) daltons. The metabolon symmetry corresponds to the D3 point symmetry group. It is supposed, that the tricarboxylic acid cycle enzyme complex interacts with other multienzyme complexes of the matrix and the electron transfer chain.

Citric Acid Cycle↗

[Study of bradykinin conformation in a dimethylsulfoxide solution by two-dimensional 1H-NMR spectroscopy].

The role of charged groups of the nonapeptide bradykinin in stabilization of its spatial structure in dimethyl sulfoxide solution was investigated. The signal assignment in the 1H-NMR spectra was achieved by means of two dimensional correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The changes in the NH and C alpha H proton chemical shifts of the Arg1 and Arg9 residues, variations both in temperature coefficients of chemical shifts of NH-resonances and coupling constants, as well as the appearance of additional NOE cross-peaks in NOESY spectra for d alpha N and d beta N 1H-1H distances were revealed by comparing the NMR spectra for two states--with the protonated C-terminal carboxyl group and deprotonated one. The experimental results are in agreement with the assumption that the conformation of the peptide in (CD3)2SO is stabilized by electrostatic interaction between the oppositely charged N- and C-terminal groups. The conformation with deprotonated alpha-carboxyl group is characterized by two beta-turns in the sequences Pro2-Pro-Gly-Phe5 and Ser6-Pro-Phe-Arg9.

Amino Acid Sequence↗

[Leptospira distribution in the soil of a natural focus of the infection (an attempt at the radioisotopic labelling of infected voles)].

The mass radioisotope (32P) labelling of all tundra voles excreting Zeptospira, infected urine was carried out over the area of 1 ha, and the results of the experiment analyzed by the method of planar coherent graphs, showed the irregular (spotted) distribution of "infected spots", i. e. soil patches contaminated by Zeptospira. The borders of individual small foci of infection did not coincide with the outlines of soil and vegetational divisions of the area. The spatial structure of the "extraorganismic" part of the Zeptospira population was originally formed by the carriers and thus determined by their mobility, their manner of using the territory. Later this structure was changed by selective elimination under the influence of living conditions for Zeptospira in the soil. The "infected spots", subjected to such selection, seem to be capable of constantly sustaining Zeptospira; the presence of these organisms is also due to the fact that they are regularly added into the soil with urine excreted by the carriers visiting the same spots of their territory.

Animals↗

[Theoretical conformation analysis of beta-casomorphin-5 molecule].

The spatial structure of beta-casomorphin-5 molecule--H-Tyr-Pro-Phe-Pro-Gly-OH has been investigated by the theoretical conformational analysis method. The results indicate that the conformational properties of the molecule can be represented by a large number of structures having twelve different backbone forms.

Caseins↗

[Cardiac children surgically treated under deep hypothermia in the first months of life. Psychopedagogic evaluation].

A psychometric and behavioral evaluation was undertaken in the first 31 patients surviving after intracardiac repair during circulatory arrest and deep hypothermia in the first months of life. I.Q. in these patients were similar to those in normal children albeit that no extrasurgical pathological condition impaired the cerebral potential (dysmaturity). Graphic and spatial structuration problems were present in half the patients around age 5-6 years. These problems disappeared later on. Functional autonomy was decreased at the beginning of school. There was no school absenteism. Participation in gymnastics was normal. One third of patients participated in sports regularly.

Activities of Daily Living↗

[Leucine aminopeptidase crystals from the bovine pancreas].

Crystals of leucinaminopeptidase from bovine pancreas were obtained. Space group (P6322), parameters of the cell a-b-132 A, c = 122 A and distribution of spot intensities on precessional X-ray patterns were in full agreement with corresponding parameters for leucinaminopeptidase crystals from bovine eye lens. A conclusion is drawn about similarity between the spatial structures of leucinaminopeptidase from bovine pancreas and eye lens.

Animals↗

[Effect of genotype on evoked potentials of the left and right hemispheres during perception of visual stimuli].

Intrapair similarity of evoked potentials (EPs) to light flash, checkerboard, house picture, the word "house" and a number of other stimuli was estimated in 20 pairs of monozygotic and 20 pairs of dizygotic adult twins of the same gender. In the right temporal area the level of the genotypic control of amplitude-temporal parameters of EPs on the whole is higher than in the left one; the more prominent this difference is in responses to spatial-structural stimuli. In the left temporal area, the genotypic control is manifested in latencies of components of EPs to linguistic stimuli.

Adolescent↗

[Conformation of the enkephalin cycloanalog Lys-Tyr-Gly-Gly-Phe-Leu-].

The spatial structure of an enkephaline cycloanalogue Lys-Tyr-Gly-Gly-Phe-Leu--has been investigated by means of energy calculations, fluorescence and CD-spectroscopy. Despite the high conformational mobility of the cycloanalogue, little resemblance exists between its and parent peptide's low-energy structures. Conformational factors for possible mechanisms of interaction between specific enkephalin receptors and cycloanalogue are discussed.

Circular Dichroism↗

[Electron microscopy of glutamine synthetase from pea leaf chloroplasts].

Using electron microscopy, the spatial structure of glutamine synthetase from pea leaf chloroplasts was studied. The enzyme was shown to consist of eight elongated subunits, which are arranged with a point of 42 symmetry at the vertices of two squares. These squares are twisted about a 4-fold axis at 40 degrees relative to each other.

Chloroplasts↗

[Conformation characteristics of gamma-glutamyl-containing peptides].

Conformational analysis, by the method of atom-atomic potentials, has been carried out for five tripeptides containing gamma-glutamyl bonds and having general formula Glu(gamma)-X-Gly. The spatial structures have been determined and the changes arising on varying the second residue have been analyzed. A comparison of possible conformations and biological activity in respect to a number of enzymes allows to conceive what structural features of these compounds are important for the substrate specificity of the enzymes. In particular, the active site topography has been surmised for glutathione synthetase (EC 6.3.2.3) and gamma-glutamyltranspeptidase (EC 2.3.2.2). The glutathione thiol group has been found to be exposed in all possible conformations that explains its accessibility for various reagents.

Binding Sites↗

[RNA polymerase-rifamycin. A molecular model of inhibition].

By fluorimetric titration of Rifs (E. coli B) and Rifr (E. coli rpoB255) RNA polymerases with rifamycin, the mutant polymerase was demonstrated to bind rifamycin. A comparison of spatial structures of rifamycin and dinucleotide fragment of RNA in the hybrid with DNA revealed their similarity. Taking into account this structural similarity and also the fact that two phosphodiester bonds can be formed by RNA polymerase in the presence of rifamycin, a model for the inhibition mode was proposed. According to this model, rifamycin occupies the place of two terminal nucleotides of synthesized, but not translocated pentanucleotide in the transcribing complex. Asp-516 of the wild type beta-subunit was assumed to form a hydrogen bond with the rifamycin C(23) hydroxyl group. On the base of this model, reduced "cycling" synthesis of tetra-, penta-... up to decanucleotides by the Rifr RNA polymerase, in comparison with Rifs, was predicted.

DNA-Directed RNA Polymerases↗

[Electron transfer in hemoproteins. VIII. Influence of ionic strength on the rate of reduction of ferricytochrome c by oxymyoglobin derivatives, chemically modified at histidine residues].

The influence of chemical modification of His residues in Mb on the rate of redox reaction in system MbO2--Cyt c has been studied at different ionic strengths and pH medium. The products of alkylation of all available His by bromacetate and iodacetamide, CM-Mb and CA-Mb, respectively, and myoglobin, modified by spin label 2,2', 6,6'-tetramethyl-4-bromoacetoxypiperidine-1-oxyl (SL) at His residue A10--Sl (His-A10)--Mb have been studied. It has been shown, that the character of the ionic strength dependence of reaction SL(His-A10)--MbO2 with Cyt c at pH 6.0 ann 7.0 is basically analogues to that, observed for intact protein. It means that only His-GH1 of two His residues, His-A10 and His-GH1, situated in the region of "active contact" of Mg with Cyt c molecule, participates in the interactions, essential for electron transfer. The interaction of the charge of this His with the negatively charged group of Cyt c is necessary, probably for the proper arrangement of other interactions in the active complex, because the deprotonation of His-GHl in the studied pH interval decreases the rate of the process by more than one order of magnitude. The rate of oxidation of MC-MbO2 and CA-MbO2 by ferricytochrome c, in contrast to intact protein, shows a weak dependence on the ionic strength and does not depend on the pH medium, throughout the range of ionic strengths from 0.005 to 1.0. The cause of the radical change in the ionic strength dependence is, probably, nearly entire disturbance of electrostatic interactions in the active complex due to chemical modification of His residues in the site of "active contact", and first of all, the His-CHl residue. The fact, that during alkylation of all available His in Mb the electron transfer persists in the system, points to that in the process of electron transfer to cytochrome c, uncharged group, most probably "inner" His-B5, participates. Based on the data on spatial structure and the obtained results, the positions of the charged groups in the site of "active contact" of Mb with Cyt c molecule are presented.

Animals↗

[Effect of arteparon on the formation of calcium phosphate and calcium pyrophosphate crystals--comparative experimental study].

The influence of Arteparon on crystal formation in vitro has been examined in calcium orthophosphate and in calcium pyrophosphate mixtures. Similar factors may be operative in the cartilage calcification processes, and thus in chondrocalcinosis. Authors have ascertained that Arteparon - like chondroitin sulphate - inhibits crystal separation. The optimal concentration for this inhibition lies in the range of the proteoglycan content of normal cartilage. Where there are physiological phosphate-pyrophosphate ratios and a low magnesium concentration, it is certain that Arteparon has an inhibiting effect. Apart from the spatial structure of the substance, the importance of negative charge density for the inhibition of separation of calcium phosphate and pyrophosphate crystals is thought to contribute to this effect of Arteparon.

Calcium Phosphates↗

[Possible use of television image analysis for measuring the volume and surface area of irregularly shaped nuclei].

A use of the TV image for measuring volumes and squares of blood cell nuclei irregular in shape is considered. The linear correspondence has been detected between the volumetric-spatial structure of the object and the shape of the respective video-signal, this correspondence being mathematically proved. The measuring of parametres of video-signal may be informative of the object's shape.

Animals↗