Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “prototype”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,657 records · Page 92Linked to original sources

Human occupational and performance limits under stress: the thermal environment as a prototypical example.

The authors wish to challenge the contemporary stress limits for workers exposed to adverse thermal conditions. Further, they wish to challenge the basis upon which all such occupational stress exposures are founded. It is their contention that task performance level should be the primary criterion for exposure. Change in behavioural performance efficiency is the most sensitive reflection of human response to stress. Such responses are superior as indices of incipient damaging effects compared with the traditional measurement of physiological function. Efficient and error-free performance is the principal criterion of contemporary work, especially in high-technology systems. Therefore, continuing exposure after work performance efficiency begins to fail, but before current physiological limits are reached, is inappropriate for both the safety and the productivity of the individual worker, their colleagues, and the systems within which they operate. Behavioural performance assessment should therefore supercede physiological assessment as the primary exposure criterion, although physiological measures still provide important supplementary information. A new description of such performance thresholds for heat stress is presented, together with its substantive theoretical foundation. Performance limits are of growing importance for prescriptions to all forms of occupational exposure and are critical necessities for future statements concerning comprehensive protective safety standards.

Efficiency↗

Microarray-based compendium of hepatic gene expression profiles for prototypical ADME gene-inducing compounds in rats and mice in vivo.

To examine species-specific aspects of the induction of absorption, distribution, metabolism and excretion (ADME)-related genes, we used 25 000 gene oligonucleotide microarrays to construct a rodent gene-response compendium that compared hepatic gene expression profiles and developed consensus aryl hydrocarbon receptor (AhR), constitutive androstane receptor (CAR) and pregnane X-receptor (PXR) ligand signatures relevant to drug clearance. Twenty-six inducer compounds were chosen from the literature. Rats and mice received one of six dose levels (log2 dose escalation, 32-fold dose range) of each compound daily for 3 days. Animals were necropsied 6-9 h after the last dose, and tissues were collected for RNA analysis. Hepatic gene expression profiles were obtained using Rosetta Resolver expression analysis system, and ADME-related genes were extracted. Cross-talk among nuclear receptors or hepatoxicity at high dose levels resulted in large signatures (usually >1000 genes at p < 0.01) for most compounds. After ADME gene transcript enrichment, agglomerative clustering separated AhR ligands from CAR/PXR ligands, but it was difficult to distinguish CAR from PXR ligands. Consensus signatures were derived from groups of AhR, CAR and PXR ligands; and cross-talk among responding genes was determined. Many compounds had distinct log dose-response profiles, and relative potencies for ligands were established. Robust responses by CYP1A1, CYP2B10 (CAR responsive in mice) and CYP2B15 (CAR responsive in rats) and CYP3A1 (PXR responsive in rats) were used to benchmark the relative potency of different ligands and to determine the relative selectivity for AhR, CAR or PXR. By using a compendium of gene expression profiles, we defined species-specific induction patterns across the ADME transcriptome.

Animals↗

The Hazard Analysis Critical Control Point's (HACCP) concept as applied to some chemical, physical and microbiological contaminants of milk on dairy farms. A prototype.

Quality management on dairy farms becomes more and more important regarding the different areas of animal health, animal welfare and food safety. Monitoring animals, farm conditions and farm records can be extended with risk identification and risk management. The hazard analysis critical control point's system is useful as an on farm strategy to control the product as well as the production process on the areas of animal health, animal welfare and food safety. This article deals in detail with the question how to develop a qualitative method where risk can be defined as an interaction between probability and impact. Two parts of the production process (milk harvest and treatment of cows) where used as an example how to apply the hazard analysis critical control point's system on chemical, physical and microbiological contaminants of milk. Not just only by summarizing the different critical checkpoints for each area but also by giving them a precise judgement of probability and impact.

Animals↗

Inhibition by ethinylestradiol of N-ethyl-N-nitrosourea-initiated uterine carcinogenesis in transgenic mice carrying a human prototype C-Ha-ras gene (rasH2 mice).

In order to demonstrate the tumor promoting effect of ethinylestradiol (EE) in our uterine carcinogenesis model, rasH2 or ICR mice given an intraperitoneal injection of 120 mg/kg body weight of N-ethyl-N-nitrosourea (ENU) or an intra-uterine injection of 50 mg/kg body weight of ENU, respectively, followed by 2.5 or 0 ppm EE in the diet for 24 weeks in experiment 1 and 6 weeks in experiment 2. In experiment 1, in ICR mice, the incidences of adenocarcinomas in the ENU alone and the ENU+EE groups were 0% and 37.5%, respectively, the difference being statistically significant. The incidences of atypical hyperplasias and endometrial hyperplasias in the ENU+EE group were also significantly higher than those in the ENU alone group. In rasH2 mice, on the other hand, no endometrial proliferative lesions were induced in the uterus of the ENU+EE group, although uterine adenocarcinomas (55.6%), atypical hyperplasias (33.3%), and endometrial hyperplasias (22.2%) were observed in the ENU alone group. Proliferating cell nuclear antigen (PCNA) positive indices for uterine adenocarcinomas and atypical hyperplasias in ICR mice treated with ENU+EE showed high values, but those in rasH2 mice given ENU alone were comparable to data for intact epithelium. In experiment 2, the immunohistochemical expression of estrogen receptor alpha (ER alpha) in the uterine luminal and glandular epithelium in the ENU+EE group of ICR mice was moderate to marked, but that in the ENU alone group was slight. There was no consistent difference in ER alpha expression in the uterine luminal and glandular epithelium between ENU+EE and ENU alone groups of rasH2 mice. These results suggest that 2.5 ppm EE paradoxically inhibits the uterine carcinogenesis in rasH2 mice initiated with ENU.

Adenocarcinoma↗

Assessment of the performance characteristics of a prototype 12-element capacitive contact flexible microstrip applicator (CFMA-12) for superficial hyperthermia.

The electrical performance of the CFMA-12 operating at 433 MHz is assessed under laboratory conditions using a RF network analyser. From measurements of the scattering parameters of the CFMA-12 on both a multi-layered muscle- and fat/muscle-equivalent phantom, the optimal water bolus thickness, at which the transfer of the energy to the phantom configuration is maximal, is determined to be approximately 1 cm. The SAR distribution of the CFMA-12 in a multi-layered muscle-equivalent phantom is characterized using Schottky diode sheets and a TVS-600 IR camera. From the SAR measurements using the Schottky diode sheets it is shown that the contribution of the E(x) component to the SAR (SAR(x)) is maximal 7% of the contribution of the E(y)component to the SAR (SAR(y)) at different layers in both phantom configurations. The complete SAR distribution (SAR(tot)) at different depths is measured using the power pulse technique. From these measurements, it can be seen that SAR(y)at a depth of 0 cm in the muscle-equivalent phantom represents up to 80% of SAR(tot). At 1 and 2 cm depth, SAR(y) is up to 95% of SAR(tot). Therefore, in homogeneous muscle-equivalent phantoms, E(y) is the largest E-field component and measurement of SAR(y) distribution is sufficient to characterize SAR-steering performance of the CFMA-12. SAR steering measurements at 1 cm depth in the muscle-equivalent phantom show that the SAR maximum varies by 40% (1 SD) around the average value of 38.8 W kg(-1) (range 10-65 W kg(-1)) between single antenna elements. The effective fieldsize (E(50)) varies by 14% (1 SD) around the average value of 19.1 cm(2).

Absorption↗

Cystic fibrosis in Arabs: a prototype from Jordan.

Cystic fibrosis is believed to be rare in Arabs. We report 202 cases (114 boys and 88 girls) diagnosed in Jordan over a period of 9 years. The mean age at diagnosis was 2.9 years. Classical presentation with growth failure, malabsorption and respiratory symptoms occurred in 75.4% of cases. Eighteen (10.8%) presented with hepatomegaly, 12 (7.2%) with meconium ileus and 11 (6.6%) had Pseudo-Bartter syndrome. Thirty-eight (23%) children died, most below the age of 1 year which may reflect a more severe disease in our population. Consanguineous marriage was present in 69% of cases. Genetic screening of 84 children and 66 parents revealed 24 different CFTR mutations with a DF508 mutation accounting for only 7.4%. Among the mutations detected, six were alleles identified for the first time. The fact that boys outnumber girls might reflect more deaths in girls due to the observed gender gap in CF mortality. It is possible that the low incidence of the DF508 mutation is due to a confounding effect and the high mortality in those carrying this mutation. The large number of different mutations reflects the ethnic diversity of the Jordanian population and the complex history of the country.

Adolescent↗

Abdominal aortic aneurysm and stent graft phantom manufactured by medical rapid prototyping.

A novel human aorta phantom built by medical rapid prototypingfor use in computed tomography (CT) scanning is described. The phantom contained a stent graft that was deployed internally to mimic a repaired aortic aneurysm. The phantom was produced to allow assessment of the CT appearance of a stent graft inside an aorta using the new virtual intravascular endoscopy image presentation technique. The stent graft utilized contained suprarenal components (metalfixation struts), and these were placed with these struts covering the renal artery ostia. The phantom was filled with iodinated contrast medium at a concentration that produced a density similar to that found in normal CT angiographic scanning. The model was scanned at a variety of slice thicknesses, pitch and image reconstruction intervals. Visualization of the stent suprarenal components in relation to the renal artery ostia is shown and the overestimation of stent wire strut diameter demonstrated.

Aortic Aneurysm, Abdominal↗

NMR studies of backbone-alkylated DNA: duplex stability, absolute stereochemistry, and chemical shift anomalies of prototypal isopropyl phosphotriester modified octanucleotides, (Rp,Rp)- and (Sp,Sp)-(d-[GGA(iPr)ATTCC])2 and -(d-[GGAA(iPr)TTCC])2.

The DNA octamer (d-[GGAATTCC])2 and four alkylated analogues, (Rp)-(d-[GGA(iPr)ATTCC])2, (Sp)-(d-[GGA(iPr)ATTCC])2, (Rp)-(d-[GGAA(iPr)TTCC])2, and (Sp)-(d-[GGAA(iPr)TTCC])2 have been examined using 1H and 31PNMR spectroscopies. Duplex stability, as monitored by both NMR and optical measurements, is shown to be a function of both site and stereochemistry of the phosphotriester moiety. Chemical shift changes relative to the native octamer indicate that there are long-range perturbations in the isopropylated molecules. 1HNMR is shown to be a general means by which stereochemistry at phosphorous can be determined.

Alkylating Agents↗

A prototype screening instrument for cannabis use disorder: the Cannabis Use Disorders Identification Test (CUDIT) in an alcohol-dependent clinical sample.

The Cannabis Use Disorders Identification Test (CUDIT) was used for the first time as part of a randomized controlled trial for brief interventions in mild to moderate alcohol-dependent out-patients. This sample may be seen as a population at increased risk of cannabis use disorder. The CUDIT was developed by modifying the Alcohol Use Disorders Identification Test (AUDIT). The ability of the CUDIT to accurately screen for cannabis abuse or dependence was examined in the portion of the sample who reported some cannabis use over the preceding 6 months (n=53), as was self-reported frequency of cannabis use in the preceding 6 months. The CUDIT was superior to the frequency measure, achieving positive predictive power of 84.6% and sensitivity of 73.3% at a cut-off of 8, compared to positive predictive power of 81.8% and sensitivity of 60.0% for 80 or more cannabis use-days. These results indicate the viability of a screening measure for identifying cannabis use disorder in at risk populations.

Adolescent↗

Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family.

Specific carrier-mediated transport of purine and pyrimidine nucleobases across cell membranes is a basic biological process in both prokaryotes and eukaryotes. Recent in silico analysis has shown that the Aspergillus nidulans (UapA, UapC) and bacterial (PbuX, UraA, PyrP) nucleobase transporters, and a group of mammalian L-ascorbic acid transporters (SVCT1 and SVCT2), constitute a unique protein family which includes putative homologues from archea, bacteria, plants and metazoans. The construction and functional analysis of chimeric purine transporters (UapA-UapC) and UapA-specific missense mutations in A. nidulans has previously shown that the region including amino acid residues 378-446 in UapA is critical for purine recognition and transport. Here, we extend our studies on UapA structure-function relationships by studying missense mutations constructed within a 'signature' sequence motif [(F/Y/S)X(Q/E/P)NXGXXXXT(K/R/G)] which is conserved in the putative functional region of all members of the nucleobase/ascorbate transporter family. Residues Q449 and N450 were found to be critical for purine recognition and transport. The results suggest that these residues might directly or indirectly be involved in specific interactions with the purine ring. In particular, interaction of residue 449 with C-2 groups of purines might act as a critical molecular filter involved in the selection of transported substrates. The present and previous mutagenic analyses in UapA suggest that specific polar or charged amino acid residues on either side of an amphipathic alpha-helical transmembrane segment are critical for purine binding and transport.

Amino Acid Sequence↗

Prototype development and comparative evaluation of wheelchair pressure mapping system.

Wheelchair pressure mapping devices used in the prescription of seat cushions and postural supports have been limited in durability, data presentation, and/or clinical efficiency. This project sought to establish the ideal specifications for clinically useful pressure mapping systems, and to use these specifications to influence the design of an innovative wheelchair pressure mapping system (Tekscan "Seat"). Technology, previously developed for measurement of forces of dental occlusion and of the foot during gait, was applied to wheelchair seat mapping. Tests were designed to compare the performance of three pressure mapping systems: the Tekscan system, the FSA system, and the Talley TPM3. Bench tests were done to measure reproducibility, hysteresis, and creep of each of the pressure mapping systems. A contoured loader gauge was developed to test for the influence of hammocking. Tests were also performed using spinal cord-injured subjects to demonstrate the relative performance of the pressure mapping systems in a clinical setting. A focus group session was conducted with seating specialists to review the strengths and weakness of the systems for routine clinical use. The TPM3 was found to be the most accurate, stable, and reproducible but limited in ease of use, speed, and data presentation. FSA was rated well in clinical application and data management but demonstrated a pronounced hysteresis (+/-19%) and creep (4%). The Tekscan system also showed substantial hysteresis (+/-20%) and creep (19%) but was preferred by clinicians for its real-time display capabilities, resolution, and display options. Some trends in system performance on varied support surfaces were identified and can be a valuable guide to interpretation of measurements and prescription decision making in the clinic. Problems identified with the accuracy and stability of the Tekscan and FSA systems may be amenable to resolution with software correction and changes in fabrication. With these improvements all three systems show the potential to be useful clinical tools.

Consumer Behavior↗

Using virtual environments to prototype auditory navigation displays.

There is a critical need for navigation and orientation aids for the visually impaired. Developing such displays is difficult and time consuming due to the lack of design tools and guidelines, the inefficiency of trial-and-error design, and experimental participant safety concerns. We discuss using a virtual environment (VE) to help in the design, evaluation, and iterative refinement of an auditory navigation system. We address questions about the (real) interface that the VE version allows us to study. Examples include sound design, system behavior, and user interface design. Improved designs should result from a more systematic and scientific method of assistive technology development. We also point out some of the ongoing caveats that researchers in this field need to consider, especially relating to external validity and over-reliance on VE for design solutions.

Auditory Perception↗