Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Complement Fixation Tests”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,639 records · Page 91Linked to original sources
PubMed1986

[Monoclonal antibodies to the Japanese encephalitis virus in ascitic hybridoma preparations].

Among 4 ascitic preparations of hybridomas producing monoclonal antibodies to the Japanese encephalitis virus (JE) hybridomas JE-4 and JE-19 had high multiplication potentials and high levels of reimplantation and transplantability in mice BALB/c. The possibility of using the complete Freund's adjuvant and pristan for priming the mice was shown. The use of pristan promoted a significant decrease in the periods of ascitic tumor development and an increase in accumulation of the cells in the ascitic tumor, in the volume of the ascitic fluid and the titers of the monoclonal antibodies. The serological assays revealed that the monoclonal antibodies produced by JE hybridomas did not react with the JE virus antigen in the hemagglutination inhibition test. However, they were highly active in the indirect immunofluorescence test. IgM of hybridoma JE-19 reacted with the JE virus antigen in the complement fixation test while monoclonal IgG produced by hybridoma JE-4 was active in the neutralization test when titrated in the cultures of the swine embryo kidney transplantable cells. The monoclonal JE antibodies did not react with the JE virus antigen in the hemagglutination inhibition test and did not bind to the antigen of the "Sofin" strain of the forest-spring encephalitis virus in the test performed with the indirect fluorescent antibody technique.

Animals↗
PubMed1975

Brucellosis: a study of five calves from reactor dams.

Five calves from Brucella abortus reactor cows bought after they had been on infected farms for two to six weeks, were reared in isolation and examined serologically for approximately six months prior to cultural and biological examination post mertem. One calf which had been serologically negative to the Rose-Bengal, serum agglutination and complement fixation tests for the last eight weeks of its life yielded a culture of Br abortus biotype 1. This serotype had also been isolated from its dam at parturition.

Agglutination Tests↗
PubMed1984

Characterization of Brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme-linked immunosorbent assay.

Rough lipopolysaccharide, extracted by a mixture of phenol, chloroform, and petroleum ether from freeze-dried Brucella ovis cells with a yield of 0.71%, contained relatively small amounts of protein and nucleic acid contaminants as compared with lipopolysaccharides from other Brucellae. The crude lipopolysaccharide was suitable as a diagnostic antigen in an enzyme-linked immunosorbent assay for the sensitive and specific detection of ram epididymitis caused by B. ovis infection. In comparative serological tests, the enzyme-linked immunosorbent assay with B. ovis lipopolysaccharide gave better identification of infections and fewer false-negative results than the enzyme-linked immunosorbent assay with sonicated antigen or the complement fixation test.

Animals↗
PubMed1964

AEROSOLS AS A SOURCE OF WIDESPREAD MYCOPLASMA CONTAMINATION OF TISSUE CULTURES.

Mycoplasma isolates were cultured from 15 antibiotic-free cell cultures obtained from a single laboratory. Complement-fixation tests showed that these isolates were antigenically related to each other but were unrelated to M. hominis type 1, M. hominis type 2, M. arthritidis, M. laidlawii type B, Mycoplasma sp. H.Ep. #2 (Barile), or M. salivarium. Examination of serum used to feed the infected cell lines revealed no Mycoplasma. Infection resulting from cross-contamination by a single Mycoplasma strain from one cell culture to another was investigated. Although the organisms were not found in the air over the work area, aerosols containing these contaminants were produced in tissue culture bottles during the trypsinization of cell monolayers. The minimal infectious dose of Mycoplasma for tissue cultures was measured, and it was determined that one organism was capable of initiating an infection in a tissue culture. The pattern of contamination and the small dose required for infection indicated that Mycoplasma contamination was spread from one tissue culture to another via aerosols. It was demonstrated that Mycoplasma can be transferred from one cell culture to another through the use of a common burette for dispensing medium.

Aerosols↗
PubMed1982

The diagnostic laboratory tests for histoplasmosis: analysis of experience in a large urban outbreak.

Of 495 patients reported in a large urban histoplasmosis outbreak, we studied 276 whose serologic tests were done in a single laboratory. Serologic test results were positive in 96% of these patients (compared with less than 5% of controls from an endemic area), cultures were positive in 22%, and special stains in 19%. The immunodiffusion test results were negative in 13% of patients who had positive findings by complement fixation, and 1% had positive results only by immunodiffusion. The complement fixation test was almost twice as sensitive as the immunodiffusion test in patients with subclinical infection. The serologic response differed significantly among the clinical syndromes with higher titers in cavitary and lower titers in disseminated disease. Factors associated with titers of 1:64 or greater to both antigens were black race and immunocompetence. High mycelial titers were also associated with more intense exposure, and high yeast titers were associated with age less than 36 years. No prognostic significance could be proved for fourfold titer rises or falls or persistence of precipitins.

Adolescent↗
PubMed1977

[Immunoprophylaxis and serodiagnostic studies of abortion in cows caused by bacteria of the Mimeae group and the genus Yersinia].

Tested was the prophylactic effect of a vaccine against abortions in cows caused by bacteria immunologically related to R Brucella organisms. Results pointed to the substantial drop in the number of abortions in the vaccinated animals. Reported are also results of serologic investigations by the complement-fixation test with cows showing abortions and sterility.

Abortion, Veterinary↗
PubMed1964

IMMUNOLOGY AND SEROLOGY OF ANAPLASMA MARGINALE. I. FRACTIONATION OF THE COMPLEMENT-FIXING ANTIGEN.

Rogers, Thomas E. (Louisiana State University, Baton Rouge), Richard J. Hidalgo, and George T. Dimopoullos. Immunology and serology of Anaplasma marginale. I. Fractionation of the complement-fixing antigen. J. Bacteriol. 88:81-86. 1964.-Studies were conducted to fractionate and purify the complement-fixation (CF) antigen of Anaplasma marginale in infected erythrocytes of cattle. Initial attempts were made to resolve the antigen from crude stromatal preparations by various chemical and physical methods. Fractionation procedures involving partial and total lipid extraction suggested that the CF antigen was lipoprotein in nature. Fluorocarbon deproteinization of stromatal antigens was also attempted. A method was developed for the preparation of a desirable Anaplasma CF antigen which involved disintegration of infected erythrocytes by sonic vibration and separation of the antigen by differential centrifugation. Antigens prepared by this method were highly specific, colorless, did not exhibit anticomplementary activity, and possessed higher titers than standard Anaplasma antigens. When density-gradient sedimentation was applied to sonic extracts of infected cells, it was demonstrated that the CF antigen could also be fractionated by this method.

Anaplasma↗
PubMed1978

[Detection of antibodies to Rickketsia prowazekii by using the antigen neutralization test].

The authors studied a possibility of using the antigen neutralization test with dry immunoglobulin typhus erythrocytic diagnostic agent for the purpose of detection of Rickettsia prowazeki antibodies. Blood sera of 315 healthy persons, 24 patients with sporadic typhus, and 18 laboratory animals immunized with R. sibirica and R. burneti, as well as with Proteus OX19 were examined. The results obtained pointed to the high specificity and sensitivity of the given serological test. A possibility of its use for antibody detection both in the typhus patients and in persons who sustained this infection in the past was demonstrated. In difference from the complement fixation test it permits to study anticomplementary sera.

Antibodies, Bacterial↗
PubMedDate not supplied

[Participation of species specific and group specific ornithosis antigens in immune reactions].

The immunogenic properties of the species-specific antigen localized in the elementary particle membrane and group-specific or inner antigen of the causative agent of ornithosis were studied. The species specific antigen was shown to induce the antibody neutralizing the infectious properties of the agent as well as those agglutinating elementary bodies, inhibiting hemagglutination, and complement-fixing antibody detectable in the direct and indirect complement-fixation tests. The results indicate that the antigens most clearly defining the species-specific properties of the causative agent of ornithosis are localized in the elementary particle membrane. These antigens may be used for differential diagnosis studies employing not only CFT but also other antibody tests.

Agglutination Tests↗
PubMed1989

Asymptomatic rotavirus infections among normal Indian children in Chatsworth, Durban.

During a 27-month survey in Chatsworth, Durban, serum from 1,041 normal Indian children, ranging in age from birth up to 13 years, was tested for the presence of anti-rotavirus antibodies by means of a complement fixation test. It was found that from an initial high positivity rate of 47.7% in the newborn, there was a sharp drop to 23.8% in the 1-2-month age group (P = 0.0009). This low positivity rate was maintained up to the age of 9-11 months, after which it rose to 46.4% in the 12-17-month age group (P = 0.0006). There was a further significant rise between the 2-3-year and 4-5-year age groups, probably reflecting rotavirus infections in nursery school and/or in the home, the latter being nosocomially acquired from younger siblings. Stool samples were obtained from 829 of the above subjects: overall, 16.2% were positive for rotavirus antigen by enzyme-linked immunosorbent assay; the highest rate (29.5%) of asymptomatic rotavirus infection was in the 12-14-month age group. The data indicate that asymptomatic infection with rotavirus is not uncommon in this community and that older children continue to be exposed to and become infected with rotavirus.

Age Factors↗
PubMed1974

Radioimmunoassay for the major structural protein of hamster type C viruses.

A radioimmunoassay for the major, group-specific antigen (p30) of hamster type C viruses was developed. The test detected approximately 5 ng of viral protein per ml and was highly specific for hamster viruses when used with homologous antibody. Comparison of three hamster viruses, two being mouse-hamster pseudotypes, in homologous and heterologous intraspecies assays, showed no evidence of type specificity for these proteins. The pseudotype viruses showed no evidence of mouse virus p30 antigenic determinants. An interspecies antigen assay employing (125)I-labeled hamster p30 and anti-feline p30 was completely inhibited by cat (feline leukemia virus), hamster, and rat viruses, to a slightly lesser degree by mouse viruses, and only poorly by RD 114 and Gibbon ape viruses. The Mason-Pfizer virus did not inhibit this assay. Hamster p30 was detected by radioimmunoassay in individual embryos from two LSH hamsters and in several adult tissues, excluding muscle at levels below that required for detection in complement-fixation tests.

Animals↗
PubMedDate not supplied

[Toxoplasmosis. Diagnostic considerations. Results of various studies].

Toxoplasmosis as a human disease is briefly described. Concerned investigations developed in Cuba and some aspects of immunological tests performed in our country are pointed out. Results of studies conducted at the National Institute of Hygiene and Epidemiology between 1974 and 1978 are disclosed, and an increase of the number of tests is evidenced. In the thesis work of Dr. G. Delgado it was proved the high sensitivity of the complement fixation test regarding toxoplasmin. For these reasons, it is desirable the decentralization of the laboratory diagnosis of this disease.

Complement Fixation Tests↗
PubMed1983

[Passive hemagglutination reaction in differentiating foot-and-mouth disease viruses].

Experiments were carried out with the use of the passive hamagglutination reaction in the differentiation of foot-and-mouth disease viruses. The investigations made use of purified sera and specific IgG antibodies obtained through column chromatography. Conjugates were prepared with the use of bis-diazotized benzidine and glutaraldehyde. In experiments with conjugates prepared with IgG and glutaraldehyde standard and reproducible results were obtained. The use of the passive hemagglutination test has led to the successful differentiation of F. M. D. viruses of various origins. Comparative investigations with the complements-fixation test and the passive hemagglutination test have shown that the latter is more readily applicable and rapid as well as more sensitive.

Animals↗
PubMed1977

[Allergic diagnosis of abortive chlamydial infection in the goat (author's transl)].

A delayed hypersensitivity test was used for chlamydiosis diagnosis in four goat flocks. The skin-test was performed in the neck by inoculating 100 microgram of purified Chlamydia from yolk sac or McCoy cells. The reactions were read 72 h after inoculation. The two antigens gave the same results. The number of doubtful reactions observed was significantly smaller in hypersensitivity than in the complement fixation test.

Abortion, Veterinary↗
PubMed1971

Sensitive matrix gel diffusion test for the detection of Australia antigen.

A matrix gel diffusion (MGD) procedure with a sensitivity comparable to the complement fixation test (CF) has been developed for detecting Australia antigen in serum. The test utilizes a thin layer of agar (0.1 mm) with an applied plastic matrix. Reactants are introduced directly onto the surface of the agar through wells in the plastic matrix. End points obtained by CF with a panel of 11 sera varied from 1:8 to 1:512. When these sera were tested by MGD, end points for detection of antigen were within one dilution of that obtained by CF.

Agar↗