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At least 163 records · Page 9Linked to original sources
PubMed1984

[Rapid serological diagnosis of parvovirus infection in dogs. Fluorescent serological demonstration of virus-specific IgM antibodies].

Rapid diagnostic assay described comprises examination of a single serum sample of the ill dog using indirect immunofluorescence test. The procedure lasts about two hours. The finding of virus-specific humoral IgM antibodies serves as evidence for an acute parvovirus infection. 84 dogs presented as patients with enteritis were examined with this method, 48 of them were seropositive for IgM antibodies.

Animals↗
PubMed1981

Serological classification of Neisseria gonorrhoeae by co-agglutination: a study of serological patterns in two geographical areas of Sweden.

Gonococcal strains, isolated in two different geographical areas of central Sweden (Stockholm and Orebro County) during two corresponding time periods, were subjected to serogrouping by the co-agglutination method with reagents covering the previously described serogroups W I, W II and W III. All strains tested could be grouped. Significant differences were found between the two geographical areas studied. Isolates from different sites on one and the same patient and strains from contact pairs gave identical results. The test results were reproducible, when the strains were exchanged between the two participating laboratories. The reagents distinguished Neisseria gonorrhoeae from other Neisseria species and other oxidase-positive bacteria. The potential of serogrouping gonococci for epidemiological studies by means of the co-agglutination method is discussed.

Agglutination Tests↗
PubMed1980

[Microbiological diagnosis of tetanus and serological verification of the immunological status. Serological monitoring in the course of therapy and prophylaxis].

The tests for tetanus aetiological diagnosis are briefly surveyed: with particular attention to Passive Haemagglutination on Turkey Red Blood Cells (P.H./T.R.B.C.), for the assessment of antitoxin serum level. The P.H./T.R.B.C. proves a valuable tool in different occasions: -in patients, to control the immunotherapy; -in subjects at risk, to apply a correct prophylaxis; -in the whole population, to verify the outcome of compulsory vaccination.

Antibodies, Bacterial↗
PubMed1980

Serological and virological studies on patients with dengue hemorrhagic fever (DHF) in Chanthaburi province, Thailand. I. Serological studies on paired sera from DHF patients by neutralization (N), hemagglutination inhibition (HI) and staining tests.

Twenty paired sera were collected from DHF patients admitted to the provincial hospital of Chanthaburi, Thailand in 1978. They were tested by the N and HI tests and a newly developed staining test against all four serotypes of dengue (DEN) virus and Japanese encephalitis (JE) virus. Four patients were demonstrated to be cases of primary DEN infection and the other 16 to be cases of secondary DEN infection. The serotypes of recent DEN infections were easily determined by the N test in cases of primary infection, but were difficult to determine in cases of secondary infection because of the strong cross reactions among DEN serotypes. These cross reactions seemed to be due to subgroup-specific antibody, which reacts with all 4 serotypes of DEN virus in the N test. However, acute phase sera from some patients with the secondary type of antibody response reacted monospecifically in the N test, indicating the serotype of the primarily infecting viruses.

Adolescent↗
PubMed1980

Serological and virological studies on patients with dengue hemorrhagic fever (DHF) in Chanthaburi province, Thailand. II. Serological characteristics of viruses isolated from DHF patients using a clone of Singh's Aedes albopictus cells.

Four strains of dengue (DEN) virus were isolated using C6/36 cells, a clone of Singh's Aedes albopictus cells, from acute phase sera of DHF patients in Thailand in 1978. The isolates grown and passaged in C6/36 cells were not neutralized appreciably by standard rabbit antisera against each of the 4 types of DEN virus, but their serotypes could be identified by complement fixation (CF) tests. After the 4th or 10th passage in suckling mouse brain (SMB), however, three of the four strains showed type-specific reactions in neutralization (N) tests against the standard antisera. In cases of secondary infection, the highest N titer in the convalescent phase sera was demonstrated not against the isolated serotype, but against the serotype considered to have caused primary infection. Moreover in cases of secondary infection, the convalescent phase sera showed a significantly lower N titer against the newly isolated strain derived from the same patient than against the corresponding prototype standard virus. This phenomenon was not observed in a single case of primary infection.

Adolescent↗