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Peripheral reticulocyte count as biologic dosimetry of ionizing radiation. Experiments in the mouse.

The flow of reticulocytes in the peripheral blood reflects the bone marrow activity and can be used for evaluation of radiation injury. Using an apparatus capable of microfluorometric quantification of reticulocytes in suspension, the peripheral blood reticulocytes of mice exposed to various doses of ionizing radiation were examined. A dose dependent reticulocytopenia was observed. The advantages of this method in biologic dosimetry over that of evaluating chromosomal aberrations, and its applicability for screening a human populations, are discussed.

Animals↗

[Modification of reticulocyte count and distribution of degree of maturity by erythropoietin in the rat].

By means of an automatic system for reticulocyte analysis (Auras) the effect of administering 50 IU of intraperitoneal erythropoietin on the number of reticulocytes and the distribution of the maturation degree of reticulocytes was investigated on Wistar rats for 28 days. A single administration of erythropoietin will lead to a rapid increase in the absolute number of reticulocytes and to changes in the distribution of the maturation degree within the first five days. The maximum of the reticulocyte number can be observed on the third to fourth day after application. Even on the second day after administering erythropoietin the measuring numbers of the distribution of maturation degree indicate maximal changes, the distribution quotient being particularly evident.

Animals↗

Flow cytometric reticulocyte counting. Parallel evaluation of five fully automated analyzers: an NCCLS-ICSH approach.

We performed a parallel evaluation of 5 automated reticulocyte analyzers. The guidelines were those proposed by the National Committee for Clinical Laboratory Standards and the International Council for Standardisation in Haematology. Duplicate analyses were performed for 225 healthy subjects and 115 patients affected by various diseases. The reference intervals were different for each method (ADVIA 120, 27-125 x 10(3)/microL [27-125 x 10(9)/L]; CELL DYN 4000, 25-108 x 10(3)/microL [25-108 x 10(9)/L]; GEN-S, 20-85 x 10(3)/microL [20-85 x 10(9)/L]; SE 9500 RET, 23-95 x 10(3)/microL [23-95 x 10(9)/L]; and VEGA RETIC, 30-130 x 10(3)/microL [30-130 x 10(9)/L]). The comparisons of percentage counts with the microscopic reference method were satisfactory for all automated methods. However, a tendency to overestimate at low counts was noted. This progressively increased from the SE 9500 RET to the VEGA RETIC. The imprecision was excellent for all the methods at normal and high concentrations. This was higher at low concentrations. When compared with the microscopic reference, the analyzers showed satisfactory sensitivity at low counts and excellent sensitivity at high counts. The overall agreement varied from 74.8% for the GEN-S to 86.1% for the SE 9500 RET.

Adolescent↗