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Results for “Pronase”

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At least 163 records · Page 9Linked to original sources
PubMed1979

Modification of a voltage-gated K+ channel from sarcoplasmic reticulum by a pronase-derived specific endopeptidase.

AK+ -selective membrane conductance channel from rabbit sarcoplasmic reticulum (SR) is studied in an artificial planar phospholipid bilayer. Membranes containing many such channels display voltage-dependent conductance, which is well described by a two-state conformational equilibrium with a free energy term linearly dependent on applied voltage. Pronase-derived alkaline proteinase b (APb), when added to the side of the membrane opposite to the SR vesicles (trans side), reduces the voltage dependence of the K+ conductance. Single-channel fluctuation experiments show that after APb treatment, the channel is still able to undergo transitions between its open and closed states, but that the probability of forming the open state is only slightly voltage-dependent. In terms of the conformational model, the enzyme's primary effect is to reduce the effective gating charge of the opening process by over 80%; a second effect of APb is to reduce the internal free energy of opening from +1.2 to +0.4 kcal/mol. The kinetics of APb action are strongly voltage-dependent, so as to indicate that the enzyme can react only with the channel's open state. The results imply that the channel contains a highly charged polypeptide region which moves in the direction perpendicular to the membrane plane when transitions between the open and closed states occur. A lysine or arginine residue in this region becomes exposed to the trans aqueous solution when the channel is in its open conformation.

Animals↗
PubMed1979

Effects of pronase on isolated chloroplasts.

Subjecting isolated spinach chloroplasts to mild proteolysis (10-minute incubation at 20 C in 500 micrograms per milliliter pronase) caused chloroplast clumping but did not affect their integrity as measured by their ability to carry out light stimulated, glycerate-3-P-dependent O(2) evolution. Transmission electron microscopy revealed no detectable differences between the control and treated plastids. Mild proteolysis inactivated exogenously added pyruvate kinase and should be a useful technique in certain enzyme distribution studies.

Journal Article↗
PubMed1995

The kinetics of the pathogenic pronase-digested renal proximal tubular antigen and antibody in rat active Heymann nephritis.

We investigated the pathogenesis of active Heymann nephritis in the rat by conducting immunofluorescent and immunoblotting studies of the pathogenic antigen and the autoantibody, and by detecting this antigen-bound IgGs. Rat IgG was detected along the glomerular basement membrane (GBM) and significant proteinuria was observed 6 weeks after the injection of rat pronase-digested tubular brush border antigen. Circulating antibody which bound only to the brush border of proximal tubules of normal rat, appeared 2 weeks after antigen injection. Eluted antibody from nephritic kidney 6 weeks after immunization bound exclusively to the brush border of the proximal tubules of normal rat kidney. Monoclonal antibody against the nephritogenic 0.3 M antigen, which bound exclusively to the brush border in the normal rat, bound to the GBM in a fine granular fashion, as well as to the brush border from nephritic rats, indicating the deposition of nephritogenic 0.3 M antigen in the GBM of nephritic rats. On immunoblotting, both the circulating antibody and eluted antibody obtained from the nephritic kidney 6 weeks after immunization recognized the 0.3 M antigen. This antigen-bound IgG appeared in circulation at 2 weeks, becoming smaller in size at 4 weeks and disappearing 12 weeks after immunization. Thus, it is suggested that active Heymann nephritis in rats was induced by deposition of the circulating 0.3 M antigen-bound IgG complexes in the subepithelial space of GBM.

Animals↗
PubMed1995

Advanced ovulation in gilts by the intrauterine application of a low molecular mass pronase-sensitive fraction of boar seminal plasma.

The shortening of the time interval between the onset of oestrus and ovulation in sows by the transcervical administration of seminal plasma was investigated in 23 German Landrace gilts, using the technique of single horn infusions (Mariensee model) in combination with the transcutaneous sonographic monitoring of ovaries. Preparative surgery comprised the detachment of the left uterine horn from the corpus, leaving the caudal end open to the peritoneal cavity but sealing the corpus wound. The left ovary was loosely tied to the ventral abdominal wall for better sonographic distinction. The animals were used in two to four consecutive cycles. After detection of oestrus by the teaser boar, the patent (right) horns were filled by transcervical infusion of 100 ml of a variety of test solutions. Ovulation was probed by transcutaneous sonography at intervals of 4 h thereafter. Native seminal plasma provoked ovulation in the ipsilateral ovary of the treated horn 10.7 h earlier than in the contralateral ovary. This effect was reduced to 7.3 h after charcoal treatment of seminal plasma; addition of 10 micrograms oestradiol restored the effect in full, while 10 micrograms of oestradiol in PBS shortened the time interval to only 3.3 h versus the control ovary. Little effect was seen with oestrone sulfate, none with prostaglandins in PBS or with PBS alone. The preliminary characterization of the nonsteroidal component of seminal plasma advancing ipsilateral ovulation after transcervical infusion suggests a proteinaceous nature. The activity resides in the 1-10 kDa fraction separated by ultrafiltration and is lost after treatment with pronase.

Animals↗