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PubMed2004

The efficiency of different light sources to polymerize composite beneath a simulated ceramic restoration.

STATEMENT OF PROBLEM: Inadequate polymerization diminishes the physical properties of composite and compromises material strength. Newly developed light-polymerizing units (plasma arc and high intensity halogen) operate at relatively high intensity and are purported to provide optimum properties to composites in a shorter time. PURPOSE: The aim of this in vitro study was to examine the polymerization efficacy of 3 different polymerization units through a simulated ceramic restoration by determining degree of monomer conversion of a composite luting Material. MATERIAL AND METHODS: A conventional halogen light, a plasma arc light, and a high-intensity halogen light were used to polymerize a composite (Variolink II) with or without catalyst. A pressable ceramic block (5 mm in diameter, 2 mm in height) was used as an interface between the polymerizing light tip and composite. The composite/veneer combination was exposed to 2 different polymerization time protocols for each polymerization unit: (1) 20 or 40 seconds for conventional halogen light, (2) 3 or 6 seconds for plasma arc light, and (3) 10 or 20 seconds (under ramp mode) for the high-intensity halogen light. Using different light units, 120 composite specimens were evaluated (n = 5). Fourier transformation infrared spectroscopy was used to determine the level of light polymerization of the resin through the ceramic material with each of the light sources immediately after polymerization or after 24 hours. Degree of conversion was calculated as a percentage of experimentally polymerized versus maximally polymerized composite. The data were analyzed by 4-factor analysis of variance (alpha = .05). Independent t tests (alpha = .05) were used to detect differences between groups defined by the specific interacting variables. RESULTS: Degree of conversion values varied with the light source (P < .05), polymerization type (P < .05), testing time (P < .01), and exposure to each light source (P < .01). Significant interactions were present between light source and polymerization type (P = .00), light source and testing time (P = .007), and polymerization type and testing time (P = .004). The degree of conversion values were significantly higher in dual-polymerized specimens after 24 hours (P < .01), but there were no significant differences in light-polymerized specimens after 24 hours. The degree of conversion values achieved were significantly lower (P < .05) when specimens were dual polymerized by conventional halogen light and measured immediately (31.59 +/- 7.76). The degree of conversion values achieved were significantly (P < .05) higher with dual polymerization by high-intensity halogen measured after 24 hours (65.06 +/- 8.14). There were no other significant differences among groups. CONCLUSION: The highest degree of conversion values of composite were achieved with polymerization by high-intensity halogen. The plasma arc light achieved similar polymerization of composite through ceramic material in a markedly shorter period than conventional halogen light.

Analysis of Variance↗
PubMed1997

Suppression by gangliosides of polymerization of glial cytoskeletons prepared from rat astrocytes: a role of sialic acid moiety.

This study investigated in vitro the effects of gangliosides on polymerization of either the depolymerized microfilament preparation (MF) or depolymerized glia filament preparation (GF) extracted separately from the crude cytoskeletal fraction of rat astrocytes. Gangliosides GM1, GM2 and GM3 markedly suppressed polymerization of both MF and GF. The concentration of GM1, GM2 or GM3 required to induce 50% inhibition of the polymerization of 7.5 micrograms MF protein/200 microliters (IC50 of GM1, GM2, or GM3) was 3.2, 2.8 or 5.6 micrograms/200 microliters, respectively. The IC50 of each ganglioside for the polymerization of 7.5 micrograms/200 microliters of GF, furthermore, was 3.3, 3.5 or 7.4 micrograms/200 microliters, respectively, suggesting that the inhibitory activities of GM1 and GM2 on polymerization of both MF and GF were greater than those of GM3. GM1, GM2 and GM3 also suppressed dose-dependently the polymerization of both actin and vimentin. The inhibitory activities of GM1 and GM2 on the polymerization of actin or vimentin were greater than GM3, as in the case of polymerization of MF or GF. The IC50S of GD1a and GT1b for MF polymerization at the same concentration were 2.2 and 1.2 micrograms/200 microliters, respectively, and those for GF polymerization were 2.7 and 1.7 micrograms/200 microliters, respectively. The IC50 of GD3 for MF polymerization was 3.9 micrograms/200 microliters, and that for GF polymerization 4.0 micrograms/200 microliters, implying that the inhibitory activities of GD3 on polymerization of both MF and GF were greater than those of GM3. The findings suggested that the inhibitory activities of gangliosides on MF or GF polymerization became greater with increasing number of sialic acid residues. AsialoGM1 suppressed neither MF nor GF polymerization, and inhibited dose-dependently the ability of GM1 to suppress MF polymerization.

Actin Cytoskeleton↗
PubMed1976

Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules.

The interactions of nucleotides and their role in the polymerization of tubulin have been studied in detail. GTP promotes polymerization by binding to the exchangeable site (E site) of tubulin. The microtubules formed contain only GDP at the E site, indicating that hydrolysis of E site GTP occurs during or shortly after polymerization. Tubulin prepared by several cycles of polymerization and depolymerization will polymerize in the presence of ATP as well as GTP. Polymerization in ATP is preceded by a distinct lag period which is shorter at higher concentrations of ATP. As reported by others ATP will transphosphorylate bound GDP to GTP. Under polymerizing conditions the maximum level of GTP formation occurs at about the same time as the onset of polymerization, and the lag probably reflects the time necessary to transphosphorylate a critical concentration of tubulin. The transphosphorylated protein can be isolated and will polymerize without further addition of nucleotide. The transphosphorylated GTP is hydrolyzed and the phosphate released during polymerization. About 25% of the phosphate transferred from ATP is noncovalently bound to the subunit as inorganic phosphate and this fraction is also released during polymerization. The nonhydrolyzable analogue of GTP, GMPPNP, will promote microtubule assembly at high concentration. GMPPNP assembled microtubules do not depolymerize in Ca concentrations several fold greater than that which will completely depolymerize GTP assembled tubules; however, addition of Ca prior to inducing polymerization in GMPPNP prevents the formation of microtubules. Thus GTP hydrolysis appears to promote depolymerization rather than polymerization. GDP does not promote microtubule assembly but can inhibit GTP binding and GTP induced polymerization. GDP does not, however, induce the depolymerization of formed microtubules. These experiments demonstrate that tubulin polymerization can not be treated as a thermodynamically reversible process, but must involve one or more irreversible steps. Exchange experiments with [3H]GTP indicate that the "E" site on both microtubules and ring aggregates of tubulin is blocked and does not exchange rapidly. However, during polymerization and depolymerization induced by raising or lowering the temperature, respectively, all the E sites become transiently available and will exchange their nucleotide. This observation does not suggest a direct morphological transition between rings and microtubules. The presence of a blocked E site on the rings explains the apparent transphosphorylation and hydrolysis of "N" site nucleotide reported by others.

Adenosine Triphosphate↗
PubMed2000

An in vitro study of microleakage of occlusal composite restorations polymerized by a conventional curing light and a PAC curing light.

With the increase in usage of bonded dentistry procedures, so comes the development of advanced technology to polymerize it. The purpose of this in vitro study was to evaluate the microleakage of sealants and resin restorations utilizing two different curing lights. The conventional Ortholux curing light (OCL) and the Plasma Arc Curing (PAC) light attached to the KCP air abrasion unit of American Dental Technologies were utilized to polymerize sealants and resin restorations on extracted third molars and premolars. Forty-eight caries-free teeth were divided into 4 groups of 12 specimens. Occlusal sealants were polymerized on groups 1 and 3. Class I composite resin restorations were polymerized on groups 2 and 4. The PAC light polymerized group 2 and the OCL was used for groups 3 and 4. Therefore a comparison between the two light sources' polymerization could be measured by evaluating the microleakage of the two sets of specimens. The PAC light polymerized the Z100 adhesive for 5 seconds and the OCL polymerized it for 10 seconds. The Z100 A-1 composite placed in two 1 mm increments was polymerized with the PAC light for 10 seconds and the OCL for 40 seconds. The teeth were thermocycled 200 times between 5 degrees C and 55 degrees C. The teeth, coated with fingernail polish excluding the occlusal surface, were soaked in 5% methylene blue for 4 hours, removed, and rinsed with water. The teeth were invested in clear resin (Castin Craft) and sectioned longitudinally using an Isomet diamond saw. Microleakage was scored "0" if no leakage was present. Scored "1" if the leakage was present to 1/2 the preparation depth. Scored "2" if the leakage was present past the 1/2 way point but not to the pulpal floor of the preparation. A score of "3" was used if the leakage reached the pulpal floor. Unpaired t-Tests were used to statistically analyze the data. A significant difference (p < 0.05) was found between groups 1 and 3. The PAC light used to polymerize the sealants in group 1 produced no microleakage. A significant difference (p < .005) was found between groups 2 and 4. The OCL produced less microleakage (16 and 20 out of 24 sections) than the resin restorations polymerized with the PAC (only 8 of 24 sections with no microleakage. The 10-second cure by the PAC light appears to be insufficient in polymerizing a class I composite resin restoration. Possibly longer exposures with the PAC would produce less microleakage. Further research needs to be performed to evaluate if the PAC light, with an intensity of 1196 mW/cm2, may be producing high levels of strain within the setting composite. This strain may be responsible for the statistically significant increase in microleakage of the restorations polymerized with the PAC light.). It appears the PAC light would be best utilized to cure sealants and/or possibly polymerize orthodontic brackets. The conventional curing light appears to remain the best choice for polymerizing class I composite restorations.

Bicuspid↗
PubMed2001

Microemulsion polymerization of styrene in the presence of a cationic emulsifier.

The principal subject discussed in the current paper is the radical polymerization of styrene in the three- and four component microemulsions stabilized by a cationic emulsifier. Polymerization in the o/w microemulsion is a new polymerization technique which allows to prepare the polymer latexes with the very high particle interface area and narrow particle size distribution. Polymers formed are very large with a very broad molecular weight distribution. In emulsion and microemulsion polymerizations, the reaction takes place in a large number of isolated loci dispersed in the continuous aqueous phase. However, in spite of the similarities between emulsion and microemulsion polymerization, there are large differences caused by the much larger amount of emulsifier in the latter process. In the emulsion polymerization there are three rate intervals. In the microemulsion polymerization only two reaction rate intervals are commonly detected: first, the polymerization rate increases rapidly with the reaction time and then decreases steadily. Essential features of microemulsion polymerization are as follows: (1) polymerization proceeds under non-stationary state conditions; (2) size and particle concentration increases throughout the course of polymerization; (3) chain-transfer to monomer/exit of transferred monomeric radical/radical re-entry events are operative; and (4) molecular weight is independent of conversion and distribution of resulting polymer is very broad. The number of microdroplets or monomer-starved micelles at higher conversion is high and they persist throughout the reaction. The high emulsifier/water ratio ensures that the emulsifier is undissociated and can penetrate into the microdroplets. The presence of a large amount of emulsifier strongly influences the reaction kinetics and the particle nucleation. The mixed mode particle nucleation is assumed to govern the polymerization process. At low emulsifier concentration the micellar nucleation is dominant while at a high emulsifier concentration the interaction-like homogeneous nucleation is operative. Furthermore, the paper is focused on the initiation and nucleation mechanisms, location of initiation locus, and growth and deactivation of latex particles. Furthermore, the relationship between kinetic and molecular weight parameters of the microemulsion polymerization process and colloidal (water/particle interface) parameters is discussed. In particular, we follow the effect of initiator and emulsifier type and concentration on the polymerization process. Besides, the effects of monomer concentration and additives are also evaluated.

Cations↗
PubMed2001

Effect of light source and time on the polymerization of resin cement through ceramic veneers.

PURPOSE: The purpose of this study was to evaluate the efficiency of 3 different light sources to polymerize a light curing resin cement beneath 3 types of porcelain veneer materials. MATERIALS AND METHODS: A conventional halogen light, a plasma arc light, and a high intensity halogen light were used to polymerize resin cement (Variolink II; Ivoclar North America Inc, Amherst, NY) through disks of veneer materials. Equal diameter and thickness disks of feldspathic porcelain (Ceramco II; Ceramco Inc, Burlington, NJ), pressable ceramic (IPS Empress; Ivoclar North America Inc), and aluminous porcelain (Vitadur Alpha; Vident Inc, Brea, CA) were used as an interface between the curing light tips and the light polymerized resin cement. The resin cement/veneer combinations were exposed to 4 different photopolymerization time protocols of 5 seconds, 10 seconds, 15 seconds, and 20 seconds for high intensity light units (Apollo 95E [Dental Medical Diagnostic Systems Inc, Westlake Village, CA] and Kreativ 2000 [Kreativ Inc, San Diego, CA]), and 20 seconds, 40 seconds, 60 seconds, and 80 seconds for conventional halogen light (Optilux; Demetron Research Inc, Danbury, CT). A surface hardness test (Knoop indenter) was used to determine the level of photopolymerization of the resin through the ceramic materials with each of the light sources. The data were analyzed by one-way analysis of variance and a post-hoc Scheffe test (p < .05). RESULTS: The data indicates that the Variolink II Knoop Hardness Number values vary with the light source, the veneer material, and the polymerization time. For a given light and veneer material, Knoop Hardness Number increases with longer polymerization times. The Kreativ light showed statistically significant differences (p < .05) between all test polymerization times. Use of this light required a polymerization time of greater than 20 seconds to reach maximum resin cement hardness. For samples polymerized with the Apollo light, there were statistically significant (p < .05) differences in surface hardness between samples polymerized at all times, except for the 15-second and 20-second times. Samples polymerized with the halogen light showed no statistically significant (p < .05) differences in hardness between polymerization times of 60 seconds and 80 seconds. CONCLUSIONS: High intensity curing lights achieve adequate polymerization of resin cements through veneers in a markedly shorter time period than the conventional halogen light. However, the data in this report indicate that a minimum exposure time of 15 seconds with the Kreativ light and 10 seconds with the Apollo 95E light should be used to polymerize the Variolink II resin, regardless of the composition of the veneer. Conventional halogen lights required a correspondingly greater polymerization time of 60 seconds.

Aluminum Oxide↗
PubMed2005

The effects of soft-start vs continuous-light polymerization on microleakage in Class II resin composite restorations.

PURPOSE: Despite the different light sources and polymerization techniques developed to eliminate it, microleakage of resin composite still remains a problem. This in vitro study was designed to compare the effects of exponential mode soft-start polymerization with those of standard and high-intensity continuous light polymerization on microleakage in Class II resin composite restorations. MATERIALS AND METHODS: Standardized Class II cavities (4 mm wide, 4 mm long, 5 to 6 mm high, 2 mm deep) were prepared in 50 extracted human molars. Specimens were divided into 5 groups (n = 10) and restored using the same hybrid resin composite (Z250), but separately polymerized by one of five different methods: group 1: continuous halogen light polymerization for 40 s; group 2: continuous high-intensity halogen light polymerization for 10 s; group 3: exponential mode soft-start halogen light polymerization for a total of 40 s; group 4: continuous LED light polymerization for 40 s; Group 5: exponential mode soft-start LED light polymerization for a total of 40 s. All specimens were thermocycled for 1000 cycles at 5 degrees C to 55 degrees C and then placed in 0.5% basic fuchsin dye for 24 h. Specimens were then rinsed, embedded in resin and sectioned longitudinally. Dye penetration at occlusal and cervical margins was rated using a scale of 0 to 4. Data was analyzed using Kruskal-Wallis and Mann-Whitney U-tests. RESULTS: Significantly lower marginal leakage was recorded with exponential mode soft-start polymerization when compared to continuous light polymerization. No significant differences in microleakage were observed between LED and halogen light polymerization. CONCLUSION: Within the limits of this in vitro study, the use of the soft-start exponential mode of LED and/or halogen light units in the polymerization of hybrid composite was found to produce significantly lower microleakage at both occlusal and cervical margins when compared to continuous light polymerization methods.

Acid Etching, Dental↗
PubMed1990

Kinetic analysis of chemotactic peptide-induced actin polymerization in neutrophils.

Definition of the kinetics of ligand-activated actin polymerization in the neutrophil is important for ultimately understanding the mechanisms utilized for regulation of actin polymerization in this non-muscle cell. To better define the kinetics of formyl peptide (fMLP)-induced actin polymerization in neutrophils we determined F-actin content at 5 second intervals after activation of human neutrophils with a range (10(-11)-10(-9) M) of fMLP concentrations. The state of actin polymerization was monitored by quantifying F-actin content with NBD phallacidin binding in both flow cytometric and extraction assays. Results demonstrate three successive kinetic periods of fMLP-induced actin polymerization in neutrophils, a lag period, a 5 second period when rate of polymerization is maximal, and a period of declining rate of actin polymerization as F-actin content approaches a maximum. The duration of the lag period, the maximum rate of polymerization, and the maximum extent of polymerization all depend upon the fMLP concentration. The lag period varies from 0 to 12 seconds and is followed in 5-10 seconds by a 5 second burst of actin polymerization when the rate is as great as 9% increase in F-actin content per second. After the 5 second burst of polymerization, the rate of polymerization rapidly declines. The study defines three distinct kinetic periods of fMLP-induced actin polymerization during which important rate-limiting biochemical events occur. The mechanistic and motile implications of kinetic periods are discussed.

Actins↗