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PubMed1990

[Observations on microvasculature of the periosteum of infant mandibular ramus by scanning electron microscope].

In this paper, a vascular casting method using methylmethacrylate was adopted. Satisfactory samples were obtained and electron microscope observations were carried out on periosteum microvasculature of infant mandibular ramus. There were significant differences in the density and arrangement of periosteal microvascular in various regions. In muscular attached region, the blood vessels showed a high density and bundle-like arrangement parallel with the direction of muscular fibers. In nonmuscular attached region, the blood vessels showed a marked low density and an irregular net-like arrangement. Between muscular bundles small arteries into periosteum were seen. Around the mandibular foramen the vessels of the periosteum formed a high density region. The clinical application of the above mentioned results were discussed.

Child, Preschool↗
PubMed1988

Blood supply to the periosteum of the canine tibia.

A study was undertaken to determine which arteries supply the periosteum of the normal adult canine tibia. The vessels were found to arise from locations distinct from the muscular vascularization. The venous drainage of the tibial periosteum had a satellite relationship with the arteries. On the surface of the periosteum, the vessels formed rectangular patterns. All surfaces of the distal end of the tibia seemed to have less distinct anastomoses of the transverse periosteal twigs, compared with the proximal end of the tibia.

Animals↗
PubMed1986

The influence of periosteum on tendon and ligament migration.

The continuity of the periosteum of the hindlimb of growing rabbits was disturbed by severing it. The femoral insertion of the pectineus muscle and the tibial attachment of the medial collateral ligament of the knee joint were examined 3-4 weeks after the operation. It was hypothesised that the cut periosteum would not shift with respect to the underlying bone in the normal manner, and that the migration of the tendon and ligament attachments would be reduced. The pectineus tendon reversed its direction of migration in 3 animals, showed histological evidence of modified migration in 8 animals, and no change was discerned in 4 animals. The medial collateral ligament in the operated limb migrated only half as much as that on the sham operated, control limb. The results are consistent with the hypothesis that traction of the periosteum in growing limb bones is responsible for the migration of these tendon and ligament attachments.

Animals↗
PubMed1986

Effect of prostaglandin E2 on rib fracture healing in beagles: histomorphometric study on periosteum adjacent to the fracture site.

A histomorphometric study was done on healing defects in the ribs of Beagles. A transverse fracture was made in the left 9th and 10th ribs. Beagles were given either ethanol vehicle (n = 6) or prostaglandin (PG) E2 orally (n = 5) for the 30-day period after surgical manipulation to time of necropsy. Double fluorescent labels to measure bone matrix mineralization were given with each of 2 fluorochrome markers--calcein before dogs were surgically manipulated and oxytetracycline hydrochloride before they were euthanatized. The objectives were to determine the effects of fracture on regional remodeling in the periosteum, the effects of PGE2 on the regional remodeling changes in the periosteum induced by the fracture, and the systemic effect of PGE2 on remodeling changes of the contralateral matching sites. The fracture in the nontreated dogs (ethanol only) stimulated remodeling activity in the periosteum with increased resorption (P less than 0.01). However, after surgical manipulation (necropsy) was done, the extent of mineralization on the bone surface was decreased and was decreased more on the nonfractured ribs (right side) than on the fractured (healing) ribs (left side) (P less than 0.05). In the treated dogs, the administration of PGE2 increased the extent of mineralization on the bone surface on the healing ribs. However, as in the nontreated dogs, the administration of PGE2 did not alter the decreasing pattern of mineralization when comparing the bone surfaces at necropsy with the bone surfaces before surgical manipulation was done.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1985

The periosteum in growth plate failure.

To investigate the periosteal contribution to the integrity of the growth plate, the authors subjected the proximal tibias of 110 male and female Sprague-Dawley rats between the ages of 25 and 65 days to axial and perpendicular forces to produce epiphysiolysis by use of a slow-loading technique and a cross-head speed of 10 mm per minute. Paired tibias of each rat were loaded under identical conditions, the only difference being the presence or absence of an intact periosteum. Mean values of body weight, tibial length, and cross-sectional areas of the growth plate after epiphysiolysis showed a linear increase with the age of the rat. No significant differences at the 5% level were observed in the tibial length and cross-sectional areas of the paired tibias. However, a consistently higher force was needed to produce epiphysiolysis with the periosteum intact. This was significant at all ages in both axial and perpendicular loading but was marked for young rats aged 45 days and under that were subjected to axial loading, where the level of significance was less than 1%. Calculated stress values showed a similar significant trend. The periosteum contributes significantly to the stiffness of the system, as shown by typical load deformation curves, and if it is divided, the gradient of the curve is clearly reduced up to epiphysiolysis. All periosteal fibers are not recruited in resisting a perpendicular force. Histologic examination of the specimens showed two patterns of failure corresponding to axial and perpendicular loading and also showed the failure path.

Animals↗
PubMed1979

[Tendinous fibers in the periosteum and bone].

The relation of muscles and bony skeleton studied in the work demonstrates a rather complex mechanism of connections existing between tendinous elements and periosteum and bone. Investigation on fixation of muscular tendons to the skeleton has demonstrated that in some cases tendinous filaments plait into the periosteum and terminate in it, while in other cases not all the tendinous filaments terminate at the level of the periosteum, but some of them penetrate into the bone. Thus, it has been demonstrated for the first time that tendinous filaments penetrate into the compact substance of the bone.

Adult↗
PubMed1980

Biochemical identification of elastin in periosteum and pericranium.

Histologic observations of elastic in periosteum have never been confirmed biochemically. Elastins were isolated, quantitated and characterized from human periosteal and pericranial tissues. Periosteum contains approximately 2.1% elastin by weight and does not appear to vary between skeletally mature and immature donors. Pericranium contains approximately 2.7% elastin by weight. These elastin contents are comparable to that reported for human skin. Amino acid analyses of the elastin from periosteum and pericranium showed the characteristic, non-polar amino acid composition of elastin. Proportions of the polar amino acids were somewhat elevated compared with elastins from the ligamentum and the aorta but were similar to those isolated from cartilaginous sources. The desmosine contents of the periosteal and pericranial elastins were comparable to those of elastins from other sources.

Adolescent↗
PubMed1993

Microvascular and cellular defects of the periosteum of osteogenesis imperfecta.

Periosteum stripped from fragments of diaphyseal long bones from osteogenesis imperfecta (OI) patients was examined both in vivo and in vitro. The OI periosteum was thickened and characteristic finding was a defective microvascular system. The vascular walls of arterioles and capillaries were thick and the lumina of many of these vessels were narrowed or occluded by hypertrophic endothelial cells and multiplication of smooth muscle cell layers. Circulatory deficiency in the OI periosteum might be regarded as one of the causes of the slender, osteopenic diaphyseal bone characteristic of the disorder. A higher proliferative activity was observed in cultured OI periosteal cells, but the cytoplasmic structure showed increased myelinoid bodies and smooth endoplasmic reticulum and decreased lysosomes and rough endoplasmic reticulum. These findings may be related to the abnormal metabolic activity of OI periosteal cells.

Adolescent↗
PubMed1995

Estrogen receptor mRNA is expressed in vivo in rat calvarial periosteum.

Estrogen deficiency is well recognized as a cause of bone loss in rats and humans. Likewise, treatment with estrogen results in prevention of this loss. Initially, this effect was thought to be indirectly mediated but, more recently, estrogen receptors (ER) have been reported in osteosarcoma cells and primary cultures originating from surgical waste, suggesting a direct effect of this steroid hormone. Detection of ER in skeletal tissues, however, has remained elusive. The purpose of this investigation was to establish the efficacy of the highly sensitive reverse-transcription polymerase chain reaction (RT-PCR) technique to detect ER in a well defined skeletal tissue (calvarial periosteum) that is responsive to the hormone. Primers were made specific to rat ER sequences. Total RNA was extracted from rat uterus, liver, spleen, and the periosteum using an organic solvent method. cDNA was synthesized from 2 micrograms total RNA. cDNA corresponding to 40 ng total RNA/sample produced intense PCR products for ER. In descending order of intensity were uterus, liver, bone, and spleen. Importantly, a similar time-course for estrogen-induced down-regulation of steady-state mRNA levels for alkaline phosphatase and osteonectin was observed in calvarial periosteum and tissues known to express estrogen receptors. These data provide in vivo evidence of ER mRNA in bone and suggest that at least some of estrogen's action on bone is directly modulated.

Animals↗
PubMed1998

Paravaginal repair of lateral vaginal wall defects by fixation to the ischial periosteum and obturator membrane.

OBJECTIVE: The aim of the study was to evaluate the anatomic basis, efficacy, and safety of a technique for correcting lateral wall vaginal defects. STUDY DESIGN: Phase I was cadaveric dissection carried out to ascertain the strength and position of structures likely to support lateral vaginal wall defects. The ischial periosteum just anterior to the ischial spine was found to be strong tissue, relatively free of nerves and vessels. In phase II, paravaginal defects were repaired by placing sutures through the arcus tendineus and underlying obturator fascia, obturator membrane, and ischial periosteum. Other defects and urinary incontinence were corrected within the same surgical setting. Forty patients were followed up for an average of 39 months (range 7-52 months). Preoperative evaluation consisted of an extensive history, cough stress test, spontaneous uroflowmetry, postvoid residual urine determination, urethral axis determination, site-specific pelvic floor defect evaluation, and multichannel urodynamic studies. After the operation patients underwent evaluations at 3 months, at 6 months, and then annually. RESULTS: Objective site-specific re-examination of the 40 patients revealed the following recurrences: lateral wall in 1 of 40 procedures, anterior wall in 3 of 35 procedures, posterior wall in 1 of 36 procedures, and apical wall in 1 of 27 procedures. Thirty-four of 36 women (94.4%) with urodynamically confirmed genuine stress incontinence or potential incontinence achieved cure (P <.001). CONCLUSIONS: (1) The ischial periosteum and obturator membrane are consistently strong reattachment sites. (2) Repair of paravaginal defects with these tissues is effective and safe. (3) Urodynamic parameters were unchanged after the operation except for measures of incontinence, which were improved (P <.001). (4) Performing other pelvic procedures did not negatively alter the success rates of paravaginal repair. (5) The urethral axis was favorably altered after the operation (P <.01).

Abdomen↗
PubMed1999

[Chronic lateral ankle instability: surgical treatment with periosteum ligamentoplasty and capsular ligament tension. 34 cases].

PURPOSE OF THE STUDY: The authors report a series of thirty four ankle ligamentoplasties treated between 1985 and 1995. MATERIAL: Patients presented an average of 10.4 ankle sprains before surgical treatment. Pre-operative examination found instability in all cases, permanent pain in 58.8 p.cent limited mobility in 11.8 p.cent and permanent oedema in 23.5 p.cent. Surgical treatment associated ligamentoplasty with periosteum and capsular ligamentar tensionning. METHODS: Minimal follow up was two years and averaged 4 years 7 months. Post operative results were assessed according to Saillant's clinical criteria (subjective instability, sprain recurrence, pain). Radiological criteria included plain and stress X-rays (anterior drawer and varus laxity). RESULTS: The authors noticed an improvement of clinical and radiographical criteria, without ankle arthrosis or periosteum graft calcification. The global result was: 23 excellent, 4 good, 1 fair and 6 bad results. All athletes went back to sport. DISCUSSION: Results of this series with almost 80 p.cent of excellent and good results are comparabie with others techniques for ankle ligament reconstruction. CONCLUSION: This simple technique has a main advantages: no injury to the fibularis brevis tendon. Periosteum ligamentoplasty can then be suggested if lateral tibio-tarsal instabilities persist after proprioceptive physiotherapy.

Adult↗
PubMed2001

[The anatomic study and clinical application of the bone (periosteum) flap pedicled with upper muscular branches of lateral femoral muscle].

OBJECTIVE: To explore the anatomic feature and clinical application of the bone (periosteum) flap pedicled with upper muscular branches of lateral femoral muscle. METHODS: The anatomic features and distribution of upper muscular branches of lateral femoral muscle were observed in the lower extremities of 40 adult cadavers. From February 1989 to February 1999, 7 cases with bone defect or nonunion of upper part of femur were treated with transfer of bone (periosteum) flap pedicled with upper muscular branches of lateral femoral muscle. RESULTS: The upper muscular branches of lateral femoral muscle originated from the transversal branch of lateral circumflex femoral artery. The musculoperiosteal branch and periosteal branch were originated at 16.8 +/- 3.0 cm below the greater trochanter. The diameter and length of musculoperiosteal branch were 1.4 to 1.7 mm and 2.7 to 5.6 cm, those of the periosteal branch were 0.4 to 0.6 mm and 1.2 to 1.5 cm respectively. Bone union achieved in 10 to 18 weeks after operation in all 7 cases after 18 to 42 months follow-up. The motion of hip joint reached 180 degrees in 4 cases, 120 degrees in 2 cases and 65 degrees in 1 case. The donor area recovered well. CONCLUSION: The bone (periosteum) flap pedicled with upper muscular branches of lateral femoral muscle is an effective alternative for repairing the bone defect or nonunion of the upper or middle part of femur.

Adult↗
PubMed1990

[Difference in responses of free nerve endings and Ruffini-type endings innervating the cat mandibular periosteum to square wave pressure stimuli, ramp mechanical stimuli and triangular vibrations].

UNLABELLED: The free nerve endings and Ruffini type endings in the cat mandibular molar buccal periosteum (posterior periosteum) were selectively stimulated with specific displacements of various velocities and frequencies and the afferent impulses generated by the nerve endings were recorded. RESULTS: 1. There were two types of fast-adapting free nerve endings, discharging on effects and off-effects in response to square wave pressure stimuli and ramp mechanical stimuli and on-effects only. 2. Unencapsulated endings were distributed near the mentale foramen and again there were two types of fast adapting endings, discharging on effects and off effects in response to square wave pressure stimuli and ramp mechanical stimuli, and on-effects only. 3. Slow-adapting free nerve endings were also observed through response to square wave pressure stimuli and ramp shaped pressure stimuli. These were difficult to differentiate from the slow adapting Ruffini-type endings. 4. Ruffini type endings were slow adapting types, exhibiting impulse frequency displacement velocity curves of F = 39.627V0.743 (F = impulse frequency, V = displacement velocity, r = 0.999, n = 12 and p less than 0.001). The number of impulse displacement curves was proportional to the displacement, with F = 1.024S0.767 (F = impulse frequency, S = displacement, r = 0.981, n = 6 and p less than 0.001). 5. Free nerve endings followed Stevens' power function law, dropping in order from fast-adapting units (10 units) to intermediate type sensory units (21 units) and slow-adapting sensory units (20 units), with 0.894 +/- 0.103, 0.684 +/- 0.022 and 0.650 +/- 0.023, respectively. Values for slow adapting Ruffini type endings followed the previous order. 6. Slow adapting free nerve endings responded to triangular vibration stimuli at 0.1 Hz to 240 Hz and the lower frequency limit of 0.1 Hz (actually it was below 0.1 Hz but this value was adopted in order to facilitate the recording of the difference to fast adapting free nerve endings) was considerably lower than the 0.6 Hz to 1 Hz range for fast-adapting free nerve endings. The structure and functions of the nerve endings in the cat posterior periosteum were investigated and it was found that the relationship between the impulse frequency and displacement velocities and displacement amplitude for slow-adapting Ruffini-type endings followed Stevens' power factor law. In conclusion, it was demonstrated that the slope constant follows the order of fast-adapting free nerve endings, intermediate type free nerve endings and slow-adapting free nerve endings.

Animals↗
PubMed2001

Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age.

OBJECTIVE: To assess the in vitro chondrogenic potential of adult human periosteum-derived cells (PDCs) with regard to the number of cell passages and the age of the donor. METHODS: Cells were enzymatically released from the periosteum of the proximal tibia obtained from adult human donors and expanded in monolayer. PDCs were harvested at multiple passages for total RNA extraction and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) gene expression analysis. For the chondrogenesis assay, cells were plated in micromass and treated with transforming growth factor beta1 (TGFbeta1) in a chemically defined medium. At different time points, micromasses were either harvested for RT-PCR analysis for cartilage and bone markers or fixed, paraffin-embedded, and stained for cartilage matrix, and immunostained for type II collagen. RESULTS: At the first 2 passages, human PDCs from young donors formed chondrogenic nodules. This spontaneous chondrogenic activity was lost upon passaging, and it was not observed in donors older than 30 years. Using a panel of marker genes, PDCs were shown to be phenotypically stable during cell expansion. Regardless of donor age or cell passage, chondrogenesis could be induced consistently by combining micromass culture and TGFbeta1 treatment. Histochemical and immunohistochemical analyses demonstrated the hyaline-like cartilage phenotype of the tissue generated in vitro. Other TGFbeta superfamily members, such as growth differentiation factor 5/cartilage-derived morphogenetic protein 1, and bone morphogenetic proteins 2, 4, and 7, were poorly chondrogenic under the same culture conditions. CONCLUSION: Adult human PDCs have the potential to differentiate toward the chondrocytic lineage in vitro, retaining this property even after extensive subculture. Human PDCs are easily accessible, expandable, and maintain their chondrogenic potential, and are therefore promising progenitor cells for use in the repair of joint surface defects.

Adult↗
PubMed1985

PGE2 stimulates both resorption and formation of bone in vitro: differential responses of the periosteum and the endosteum in fetal rat long bone cultures.

The ability of PGE2 to stimulate bone resorption in vitro and in vivo is well established but the effects of this compound on bone formation are still controversial. Recent clinical reports have suggested that long-term infusion of PGE in infants with cyanotic heart diseases led to a stimulation of periosteal bone formation and to hyperostosis. In the present report, we describe the effects of PGE2 (10(-5) M) in bone organ cultures on bone resorption, measured by the release of 45Calcium and the number of osteoclasts in sections of cultured bones, and bone volume, by measuring separately medullary and cortical areas. PGE2 induced a marked increase in 45Ca release and in cortical and medullary osteoclast numbers over 4 days in vitro; despite this increase in bone resorption, cortical bone volume remained constant, indicating a parallel increase in bone resorption and formation at this site. Morphological and quantitative data demonstrated a higher extent of osteoblastic surface along the periosteum of PGE2-treated bones when compared with control cultures. Medullary bone volume, on the other hand, decreased sharply during the culture period, demonstrating a lack of parallel increase in bone formation at this site. It is concluded that, under these experimental conditions, prostaglandin E2 stimulated both resorption and formation along the periosteum and only bone resorption along the endosteum of the cultured bones. The overall effect of PGE2 on bone as a whole, however, was net bone loss.

Animals↗
PubMed1986

Differentiation kinetics of osteoclasts in the periosteum of embryonic bones in vivo and in vitro.

Osteoclast progenitors are seeded via the blood stream in the mesenchyme surrounding embryonic long bone models long before the appearance of multinucleated osteoclasts. The proliferation and differentiation of these progenitors in embryonic mouse metatarsal bones was studied with acid phosphatase (AcP) histochemistry and 3H-thymidine autoradiography. In vivo, tartrate-resistant, acid phosphatase-positive, mononuclear cells appear in the periosteum (AcPP-P cells) at the age of 17 days (after conception). On day 18, AcP-positive, multinucleated osteoclasts invade the bone rudiment and start resorbing the calcified cartilage matrix, resulting in the formation of the marrow cavity. The kinetics of osteoclast formation in vitro was studied in metatarsal bones of embryonic mice of different ages cultured in the continuous presence of 3H-thymidine. In young bones (15 days), mainly proliferating, 3H-thymidine-incorporating progenitors gave rise to AcPP-P cell and osteoclast formation. In older bones (16 and 17 days) osteoclasts were progressively more derived from postmitotic, unlabeled precursors. Irradiation of the metatarsal bones with a radiation dose of 5.0 Gy prior to culture resulted in a selective elimination of the proliferating progenitors, whereas the contribution of postmitotic precursors in AcPP-P cell and osteoclast formation remained unchanged. The results demonstrate that in the periosteum of embryonic metatarsal bones a shift occurs from a population composed of proliferating osteoclast progenitors (15 days) to a population composed of postmitotic precursors (17 days) before multinucleated osteoclasts are formed (18 days). Obviously, postmitotic AcP-negative precursors, already present in 16-day-old bones, differentiate into precursors characterized by tartrate-resistant AcP activity, the preosteoclasts (17 days), which in their turn fuse into osteoclasts.

Animals↗
PubMed1988

Direct transformation from quiescence to bone formation in the adult periosteum following a single brief period of bone loading.

The concept of resorption preceding formation in a coupled response is well established as the normal sequence of remodeling in adult bone. So prevalent is this concept, however, that the idea of the direct activation of osteogenic modeling in normal adult bone is often ignored. This experiment documents the direct transformation of the normal, quiescent, adult periosteum to active bone formation. The osteogenic stimulus was provided by a single short period of dynamic loading. Periosteal activation and the production of new bone within 5 days of loading was unaccompanied by resorption or the presence of osteoclasts. We therefore conclude that an adult resting periosteum can become directly converted to formation as a physiologic response to an appropriate osteogenic stimulus without the need for resorption. To distinguish this process from remodeling we suggest it be called renewed modeling. It is notable that a single short exposure to an "osteogenic" loading regime can influence the full cascade of cellular events between quiescence and active bone formation.

Animals↗
PubMed2003

Chemical sympathectomy-induced changes in TH-, VIP-, and CGRP-immunoreactive fibers in the rat mandible periosteum: influence on bone resorption.

The expression of neurotransmitter receptors by bone cells supports the concept that the nervous system is a regulator of bone metabolism. The discrimination of the respective roles of the sensory and sympathetic nervous systems requires evidence of topographic relationships between the corresponding fibers and the cells involved in bone turnover, in vivo. In this study, the influence of the sympathetic system on bone resorption was assessed by using a synchronized model of cortical resorption along the mandible. The sympathetic system was destroyed by daily injections of guanethidine (40 mg/kg) for 25 days; a resorption wave was induced on day 21. The distribution of periosteal tyrosine-hydroxylase (TH)-, vasoactive intestinal polypeptide (VIP)-, and calcitonin gene-related peptide (CGRP)-immunoreactive (IR) fibers was studied by compartmentalizing the periosteum. Most fibers were located in the distal, non-osteogenic compartment. TH-IR fibers were located perivascularly, VIP-IR fibers were gathered at the boundary with the osteogenic compartment, and CGRP-IR fibers were scattered. Sympathectomy decreased the number of TH- and VIP-IR fibers and increased the number of CGRP-IR fibers, without changing their topography. After the injection of Fast blue, a retrograde fluorescent marker, over the periosteum, fluorescent neuronal cell bodies were found in the superior cervical ganglion (SCG). Many neurons were TH-IR and very few were VIP-IR. Sympathectomy decreased the numbers of fluorescent and TH-IR cell bodies. It also decreased the number of preosteoclasts and osteoclasts, which had a drastic effect on the cortical bone surface, as assessed by scanning electron microscopy. These data indicate that VIP-IR fibers have a strategic position close to the most peripheral and less differentiated, osteogenic cells, pointing to a functional relationship. As poorly differentiated osteogenic cells support preosteoclast differentiation, VIP-IR fibers may be involved in this process, as suggested by the smaller number of preosteoclasts in sympathectomized rats. Although VIP is predominantly a parasympathetic mediator, it seemed to be conveyed by sympathetic fibers, as shown by the marked effect of guanethidine treatment. Nevertheless, these fibers did not originate from the SCG, contrary to TH-IR fibers.

Adrenergic Fibers↗