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Orosomucoid (ORM1 and ORM2) types in the Spanish Basque Country, Galicia and northern Portugal.

The genetic variation of orosomucoid (ORM1 and ORM2) in three south-western European populations (Galicia, Spanish Basque Country and northern Portugal) was investigated using hybrid isoelectric focusing. Three common ORM1 alleles were observed in these populations, the frequencies of ORM1 *S observed in Galicia and northern Portugal being the highest found among populations of European origin. Rare variants were observed for both the ORM1 and ORM2 loci.

Gene Frequency↗

Long-term peritoneal dialysis experience in Portugal.

Peritoneal dialysis (PD) penetration varies widely. Since the beginning of this therapy, indications have changed and outcomes have improved. In Portugal, PD still remains clearly underutilized. The results of a 20 year PD programme were evaluated: 312 cumulative patients, 48+/-16 years, 27%>60 years old, 27% diabetic, 59% with prior hemodialysis (HD). The main reason for admission was vascular access failure (48.7%). Admission due to patient preference has increased significantly between first and second decades of the programme (33% vs 47% (P<0.001)); 98 patients (31.4%) were treated with automated PD but this prescription increased to 43% of the active patients.A total of 376 Tenckhoff catheters were surgically implanted, recently by the Popovich-Moncrief technique (77 catheters): the cumulative survival was 82%, 64% and 50% at 1, 3 and 5 years,respectively. A better catheter survival was found in the last decade (85.7%, 69.6%, 54.8% versus 77.3%, 55.5%, 40.2%, at 1, 3 and 5 years, respectively (P=0.007). The patient and technique cumulative survivals were 91, 74, 55% and 85, 67, 41%, at 1, 3, and 5 years, respectively. The main drop-out was to hemodialysis (35.8%), followed by death (23.7%), and transplantation (21.5%). Peritonitis and access-related infections caused 35% of the transfer to HD. Cardiovascular events caused 58% of deaths. The median PD retention was 35.5 months. The rate of peritonitis has decreased to one episode /30 patient months. Hospital admission has also decreased to 4.8 days/patient year. This is a first report on long-term PD experience in Portugal. It has been an effective modality of renal replacement therapy, reflected by the growing patient preference in our PD programme.Experience, knowledge and new technical solutions have improved the outcomes.

Adolescent↗

Hospital pharmacy in Portugal and its future trends.

The authors begin by presenting a brief historical summary of hospital pharmacists' activities in Portugal, especially since the 1950s. They state their views on the present situation of hospital pharmacy in various aspects, namely: general legislation; activities and premises of pharmacy services; pharmaceutical career and staff recruitment; the National Hospital Formulary of Medicines and Pharmacy and Therapeutic Committees; acquisition, production and distribution of medicines; and clinical pharmacy activities. Future trends of hospital pharmacy in Portugal are summarized, stressing the following points: evolution of production and information, unit dose distribution, and steady increase of other clinical pharmacy activities.

Formularies, Hospital as Topic↗

Theatre nursing in Portugal.

A recent opportunity to visit Porto, in Portugal, allowed Allyson Lipp to observe operating department nursing in that city. In this article, Allyson explains the background to her visit, identifies some of the practices observed and compares the operating department she visited in Portugal, and nursing in that country, with their counterparts in the UK.

Humans↗

Human exposure to Anaplasma phagocytophilum in Portugal.

A retrospective study to detect Anaplasma phagocytophilum antibodies by indirect immunofluorescence (IFA) and Western blot (WB) assay was conducted in 367 potentially exposed patients from Portugal. The study included 26 patients with confirmed Lyme borreliosis (LB), 77 with suspected LB, 264 seronegative patients studied for possible tick-transmitted LB and boutonneuse fever (LB/BF) infection, and 96 healthy blood donors. Overall, patients with LB and suspected LB (n = 2 [7.7%] and n = 6 [7.8%], respectively) were more often seropositive (n = 8 [7.8%]; P < 0.001), whereas only 1 (0.4%; P = 0.046) patient in the LB/BF seronegative group had confirmed disease. This study is the first evidence of human exposure to A. phagocytophilum or an antigenically similar bacterium in Portugal, and suggests that LB patients are significantly more likely to contact A. phagocytophilum.

Anaplasma phagocytophilum↗

Ticks and tick-borne Rickettsiae surveillance in Montesinho Natural Park, Portugal.

This study constitutes the first contribution to the knowledge of tick dynamics and its implication in the epidemiology of rickettsial diseases in Montesinho Natural Park (MNP), Bragança district of Portugal. Of 76 ticks collected, 12 (15.8%) were Dermacentor (D.) marginatus, 36 (47.4%) D. reticulatus, and 28 (36.8%) Rhipicephalus (R.) sanguineus. Isolation assays were performed by shell-vial technique on 41 ticks. Israeli spotted fever strain was an isolate from R. sanguineus, and three isolates of Rickettsia slovaca were obtained from D. reticulatus. All 76 ticks were screened by PCR for Rickettsia sp., Ehrlichia (E.) chaffeensis, and Anaplasma (A.) phagocytophilum. Rickettsia RpA4 strain DNA was detected in 10 D. marginatus and 2 D. reticulatus, and Israeli spotted fever strain in 1 R. sanguineus. No E. chaffeensis or A. phagocytophilum infection was detected. New host records are provided for D. reticulatus. Also described for the first time in Portugal is the isolation of R. slovaca from D. reticulatus and the isolation of Israeli spotted fever strain from R. sanguineus. This confirms the association of the last rickettsiae strain with the same vector tick as previously described in Israel and Sicily.

Animals↗

Hyperparathyroidism-jaw tumor syndrome in Roma families from Portugal is due to a founder mutation of the HRPT2 gene.

The hyperparathyroidism-jaw tumor (HPT-JT) syndrome is an autosomal dominant disorder characterized by the occurrence of parathyroid tumors and ossifying jaw fibromas. The gene causing HPT-JT, HRPT2, is located on chromosome 1q31.2 and consists of 17 exons that encode a 531-amino acid protein, designated parafibromin. We recently identified six Roma families in Portugal with 56 members (11 affected and 45 asymptomatic), who had the HPT-JT syndrome. We postulated that they may have a common ancestor and that the HPT-JT syndrome may be due to a mutation of the HRPT2 gene. Haplotype analysis using 14 chromosome 1q24-q32 polymorphic markers showed that the 11 affected individuals shared a common haplotype defined by seven markers that spanned an approximately 12.5-cM region, flanked centromerically by D1S202 and telomerically by D1S306. DNA sequence analysis identified a 2-bp (TG or GT) frameshift deletion in exon 8, which predicts a truncated parafibromin protein, in all 11 affected individuals. This mutation was also found in 19 unaffected individuals (age range, 12-74 yr) who shared the affected haplotype, suggesting a low age-related penetrance for HPT-JT in these families. Thus, the HPT-JT syndrome in six Roma families from Portugal is due to a novel founder mutation in the HRPT2 gene.

Adult↗

[Rickettsial diseases in Brazil and Portugal: occurrence, distribution and diagnosis].

The present study is an update review on the occurrence and diagnosis of rickettsial diseases in Brazil and Portugal, aiming at promoting their epidemiological surveillance in both countries. A literature review was carried out and unpublished data of laboratories and surveillance systems were presented. The results described the occurrence of rickettsial diseases and infections in Brazil and Portugal, including other new and still poorly understood rickettsial infections. Current diagnostic methods were discussed. As in many other countries, rickettsial diseases and infections seem to be an emerging public health problem. Treated as a minor problem for many decades, the interest in these infections has increased in both countries but further studies are needed to establish their role as a public health problem.

Animals↗

[Politicized sexualities: AIDS activism and sexual orientation in Portugal].

This article analyzes how both the Portuguese state and civil society have addressed the AIDS issue, focusing on participation by the lesbian, gay, bisexual, and transsexual community (LGBT) in the struggle against AIDS. The article begins by describing the situation of LGBT individuals in Portugal during the 1990s, when the Portuguese LGBT movement emerged and grew, and then characterizes the evolution of HIV/AIDS in Portugal, analyzing the relationship between civil society and sexuality in general and the situation of the epidemic in the country in particular, considering key facts, nongovernmental organizations, and state initiatives. Special attention is given to the role played by LGBT organizations in the struggle against HIV. Finally, the article reflects on the future of the struggle against both AIDS and discrimination in the 21st century, considering recent events in the country and the guidelines recommended by international policies.

Acquired Immunodeficiency Syndrome↗

Characterization of Toxoplasma gondii isolates in free-range chickens from Portugal.

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 225 free-range chickens (Gallus domesticus) from Portugal was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 61 chickens with titers of 1:5 in 8, 1:10 in 6, 1:20 in 3, 1:40 in 23, 1:80 in 5, 1:160 in 4, 1:320 in 8, and 1:640 or higher in 4. Hearts, leg muscles, and brains of 15 seropositive (MAT 1:10 or higher) chickens were bioassayed individually in mice. Tissue from 38 chickens with titers of 1:5 or less were pooled and fed to a T. gondii-free cat. Feces of the cat were examined for oocysts, but none was found. Toxoplasma gondii was isolated from 16 of 19 chickens with MAT titers of 1:10 or higher. Genotyping of 12 of these 16 isolates with polymorphisms at the SAG2 locus indicated that 4 were type III, and 8 were type II. None of the isolates was lethal for mice. Phenotypically, T. gondii isolates from chickens from Portugal were different from those of T. gondii isolates from chickens from Brazil.

Agglutination Tests↗

Chemometric modeling of main contamination sources in surface waters of Portugal.

Various chemometric data analysis methods, such as principal components analysis, multivariate curve resolution-alternating least squares, parallel factor analysis, and Tucker3, are proposed and compared for the resolution and modeling of main contamination sources in a large environmental data array obtained in an exhaustive environmental monitoring program that examined the quality of surface waters of Portugal. The study covered the analysis of 19 priority semivolatile organic compounds (SVOCs) frequently found in a total number of 644 surface water samples, including 46 different sites from throughout Portugal and corresponding to a period of 14 months, from April 1999 to May 2000. Main contamination sources of the analyzed SVOCs were identified and interpreted according to their chemical composition and according to their resolved geographical and temporal distribution profiles.

Environmental Monitoring↗

Influence of pharmacists' attitudes on adverse drug reaction reporting : a case-control study in Portugal.

INTRODUCTION: Pharmacists can play a fundamental role in adverse drug reaction (ADR) reporting, although the factors that affect underreporting among these professionals are unknown. The objectives of this study were to identify (i) professional or demographic characteristics; and (ii) attitudes associated with pharmacists' ADR reporting in northern Portugal. METHODS: We conducted a case-control study on a population of pharmacists employed in hospital and community pharmacies across Portugal's Northern Regional Health Authority catchment area in 2003. Cases (n=34) comprised pharmacists who had reported at least one ADR to the northern region's drug surveillance unit, and controls (n=280) were randomly sampled from pharmacists who had never reported an ADR. All were interviewed using a mail questionnaire. Most attitudes were based on Inman's 'seven deadly sins' and were measured using a continuous visual analogue scale. Answers were recorded in a range from 0 (total disagreement) to 10 (total agreement). Logistic regression was used to determine the ADR reporting adjusted odds ratio (OR) for a change in exposure corresponding to the interquartile range for each attitude. RESULTS: The response rate was 86.8%. Reporting probability proved higher among hospital versus community pharmacists (adjusted OR 20.0; 95 CI 3.3, 125.0; p<0.001). Attitudes to ADRs were strongly associated with reporting probability. Hence, an interquartile decrease in any of the following attitudes increased the probability of reporting by (i) 223% (95% CI 51, 595; p < 0.05) for "Really serious ADRs are well documented by the time a drug is marketed"; (ii) 240% (95% CI 89, 508; p=0.002) for "I would only report an ADR if I were sure that it was related to the use of a particular drug"; (iii) 316% (95% CI 44, 1104; p=0.010) for "It is only necessary to report serious or unexpected ADRs"; and (iv) 171% (95% CI 13, 549; p=0.020) for "I do not have time to think about the involvement of the drug or other causes in ADRs". CONCLUSIONS: ADR under-reporting is strongly associated with certain attitudes, possibly indicating that under-reporting could be minimised through educational interventions targeted at changing such attitudes. Pharmacists' ADR education must be improved and educational programmes should be focused on altering attitudes identified by the study as being associated with under-reporting. Our data also indicate that community pharmacists must be a priority target for this intervention.

Adverse Drug Reaction Reporting Systems↗

Asthma mortality in portugal : impact of treatment with inhaled corticosteroids and leukotriene receptor antagonists.

OBJECTIVE: To determine whether the use of inhaled corticosteroids or leukotriene receptor antagonists (LTRAs) has had an impact on asthma mortality in Portugal during the period 1991-2001. METHODS: A population-based ecological study was conducted for the period 1991-2001. Yearly asthma death rates were computed for all ages. Data on sales of inhaled corticosteroids and LTRAs were obtained and expressed in defined daily doses (DDDs)/year. The association between the yearly rate of asthma deaths and consumption of these medications was estimated using Poisson regression. RESULTS: The rate of asthma death decreased steadily from 39.4 per million inhabitants in 1991 to 14.2 in 2001. At the same time, the use of inhaled corticosteroids in the population increased from 5.8 to 22.2 million DDDs per year. The adjusted rate ratio of asthma death was 0.85 (95% CI 0.78, 0.92) for every additional 5 million DDDs of inhaled corticosteroids per year and 0.84 (95% CI 0.70, 1.02) for every additional 5 million DDDs of LTRAs per year. CONCLUSION: The increasing use of inhaled corticosteroids and leukotriene receptor antagonists during the 1990s in Portugal appears to have contributed to the reduction in asthma mortality in that country.

Administration, Inhalation↗

Portugal's contribution to navigation and discovery of the New World.

Even before the birth of Prince Henry the Navigator (1394) Portugal had displayed a maritime calling due to its 500-mile shore line and numerous natural bays. Inspired by the riches of India he saw during the Portuguese exploration along the coast of West Africa, Prince Henry set out methodically to collect information by bringing together Jews and Moors with geographical knowledge to found his School of Navigation. From 1415 until his death in 1460 he attracted to his school the foremost contemporary scholars in mathematics, astronomy, and cartography along with experts in knowledge of the compass, astrolabe, water currents, and the winds. This concentration of talent yielded the invention of the caravel, the most important navigational advancement of the time, crucial for long voyages across the high seas. Although the rest of Europe busied itself in political and religious wars, for 70 years (1415-1492) Portugal alone pursued the discovery of the Atlantic. "No nation in the 15th century exhibited so great a spirit of maritime enterprise as the Portuguese."

Geography↗

Caries prevalence of young adults in Oslo, Norway, and Porto, Portugal. A comparative analysis.

The purpose of the present study was primarily to establish the oral health status of young adults in the area of Porto, Portugal. The assessment is based on a random sample of 30- to 39-year-olds with criteria identical to those of a Norwegian study of 35-year-olds. This makes it possible also to present a comparative analysis of the caries prevalence in Oslo, Norway, and Porto, Portugal. The results indicate lower DMF scores among the Portuguese (DMFS = 46.2) than the Norwegian (DMFS = 85.0) adults. The difference is primarily due to a greater number of filled surfaces among the Norwegians (FS = 59.7) than the Portuguese (FS = 4.4). However, carious surfaces are more prevalent among Portuguese than Norwegian adults (DS = 9.2 versus DS = 3.3). Both among Portuguese and Norwegian adults, oral hygiene and dental visits seem to play an important role with regard to the prevalence of decayed surfaces. Decayed surfaces were more prevalent among men than women, and a correlation between social status and prevalence of decayed surfaces was present in both societies.

Adult↗

Summary of results of a comparative study of alcohol problems and solutions: Spain and Portugal.

This study explores through a mail questionnaire alcohol problems and solutions in Spain and Portugal including: drunk-driving, treatment services and prevention, economic issues, alcohol consumption, cultural aspects, and supply of alcoholic beverages. Consumption of alcohol in both countries is lower for men and women with college educations. Marital status has minimal effects on the consumption level of men and women in Spain and of men in Portugal. For Portuguese married women, consumption is higher. Too many bars and feasts are primary cultural adjuncts contributing to alcohol problems.

Accidents, Traffic↗

Neisseria meningitidis C:2b:P1.2,5 with intermediate resistance to penicillin, Portugal.

For 1 year, serogroup, serotype, serosubtype, and penicillin susceptibility of meningococci circulating in various regions in Portugal were evaluated. Most frequent phenotypes were B:4:P1.15 (13.4%) and C:2b:P1.2,5 (75.9%), which are also common in Spain. Overall, 27.5% of C:2b:P1.2,5 strains showed intermediate resistance to penicillin. Laboratory-based surveillance of meningococcal infection in Portugal provides important information to assess the adequacy of public health measures.

Humans↗

[The French influence on the reception of the homoeopathy in Portugal].

The authors show how France has influenced in a decisive manner the reception of the homoeopathy in Portugal. They have studied the acclamation the Hahnemann by the Medical Sciences Society of Lisbon (1839) and the controversy originated by that event for the homoeopathy institutionalization in Portugal.

Attitude to Health↗