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At least 163 records · Page 9Linked to original sources

PDMS microfludic device for optical detection of protein immunoassay using gold nanoparticles.

A simple but highly specific immunoassay system for goat anti-human IgG has been developed using gold nanoparticles and microfluidic techniques. The assay is based on the deposition of gold nanoparticles that are coated with protein antigens in the presence of their corresponding antibodies to microfluidic channel surface. The effects of time accumulation, the flow velocity, and the concentration of antibodies to the red light absorption percentage (RAP) of deposition were investigated with an ordinary optical microscope. By controlling the reaction time and flow velocity, a dynamic range of 3 orders of magnitude and a detection sensitivity of 10 ng ml(-1) of goat anti-human IgG were achieved. Because of its simplicity and flexibility, this new technique should be useful for fast, highthroughput screening of antibodies in clinical diagnostic applications.

Animals↗

Resonant coupling between bulk waves and guided modes in a dielectric slab with a thick holographic grating.

What we believe to be a new type of resonant coupling of an incident bulk wave into guided modes of a slab with a thick holographic grating is shown to occur in the presence of strong frequency detunings of the Bragg condition. This happens through the reflection of the strongly noneigen +1 diffracted order with the slab-grating boundaries, the resultant reflected waves forming a guided slab mode. Rigorous coupled-wave analysis is used for the numerical analysis of the predicted resonant effects. Possible applications include enhanced options for the design of multiplexing and demultiplexing systems, optical signal-processing devices, optical sensors, and measurement techniques.

Journal Article↗

Large phase shifts in As2S3 waveguides for all-optical processing devices.

A large phase shift of 4.7pi at 1.53 microm has been observed from a low-loss (0.2 dB/cm), small-core As2S3 waveguide fabricated by dry etching. The strength of the nonlinear response was limited by photosensitivity and photocrystallization of the As2S3 films at 1.53 microm, far below the material bandgap.

Journal Article↗

Anterior chamber depth measurements in phakic and pseudophakic eyes: Pentacam versus ultrasound device.

PURPOSE: To compare anterior chamber depth (ACD) measurements with a new optical device with those taken with a standard ultrasound (US) device in emmetropic phakic and pseudophakic eyes. SETTING: Department of Ophthalmology, Medical Health and Science Center, University of Debrecen, Debrecen, Hungary. METHODS: Forty-two phakic and 42 pseudophakic patients with normal axial lengths (mean 22.91 mm +/- 1.21 [SD]) were enrolled in the study. The ACD was measured 3 times with Scheimpflug-based Pentacam (Oculus) and then 3 times with a standard A-scan US device (AL-2000, Tomey). The data were then analyzed. RESULTS: In the phakic group, the mean ACD was 2.87 +/- 0.4 mm with the Pentacam and 2.89 +/- 0.49 mm with ultrasound A-scan (US) (P = .84). In the pseudophakic group, the mean ACD was 3.41 +/- 0.28 mm and 3.97 +/- 0.45 mm, respectively (P < .001). The correlation between measurements was significant in both the phakic and pseudophakic groups (r = .547/P < .001 and r = .404/P = .01, respectively). CONCLUSIONS: In phakic eyes, ACD measured with the Pentacam and with US was the same. However, in pseudophakic eyes, the difference was significantly lower when the ACD was measured with the Pentacam. Therefore, in pseudophakic patients, further evaluation of ACD data with the Scheimpflug-based system is necessary.

Aged↗

Intraocular pressure and eye enlargement in chicks.

In chicks, the application of optical devices that blur vision results in eye enlargement and ametropia. The research presented here investigated changes in intraocular pressure (IOP), specifically intravitreal pressure, that occur with eye enlargement induced by this means. Plastic goggles (domes) were glued over one eye in 25 domestic chicks. Plastic rings were glued around one eye in 25 control chicks to control for the mechanical effects of the treatment but which left their vision undisturbed. Following two weeks of treatment, domes and rings were removed and IOP was measured. Mean IOP of the domed eyes was 1.14 mm Hg lower than that of control eyes, a difference that was not statistically significant. The eyes of an additional group of 20 chicks that experienced similar treatments were refracted; these refractions revealed that the dome-treated eyes had a mean refractive state that was 7.98 D myopic relative to the ring controls, which was statistically significant. The results indicate that IOP does not change significantly in chicks with experimental eye enlargement induced by retinal-image degradation.

Animals↗

Clinical experience with the Tscherning aberrometer.

PURPOSE: With the aberrometer based on Tscherning's principle, measurements of wavefront aberrations of human eyes with high accuracy and reproducibility are available for standard diagnostic investigations. METHODS: During investigational and clinical trials, wavefront-aberrations of about 300 human eyes were measured and evaluated within the last few years. RESULTS: measurements are presented in terms of Zernike coefficients and as height maps that can be converted directly to ablation profiles for wavefront-guided laser treatments. CONCLUSION: The Tscherning aberrometer is a simple optical device with high accuracy appropriate for routine clinical investigations on optical aberrations of the human eye.

Adolescent↗

Monitoring of heart and respiratory rates in newborn infants using a new photoplethysmographic technique.

OBJECTIVE: A new photoplethysmographic (PPG) device for respiratory and heart rate monitoring has been evaluated in the neonatal care units at the University Children's Hospital of Uppsala, Sweden. The purpose of this study was to compare this new device with more established techniques, i.e., transthoracic impedance plethysmography (TTI) for monitoring of respiratory rate and ECG for heart rate monitoring. METHODS: Data were acquired continuously for 8-hours in each of 6 neonates. The signals were analysed for periods of 30 seconds, in which the heart and respiratory signals from the PPG device were compared with the ECG and the impedance plethysmogram. RESULTS: The ECG recordings were of high quality in 77% of the analysed periods. In these periods, excluding periods (6%) disturbed by offset-adjustement of the PPG signal, the PPG heart signal included 1.1% (+/- 0.7% SD) false negative beats and 0.9% (+/- 0.6%) false positive beats. In periods with an impedance signal of high quality (29% of total time), the part of the PPG signal synchronous with respiration included 2.7% (+/- 1.1%) false negative breaths and 1.5% (+/- 0.4%) false positive breaths. Here, 2% of the periods were discarded because of offset-adjustment. From the periods of low signal quality, two other conclusions were drawn: 1) The impedance signal contains more power in the respiratory range than the corresponding PPG respiratory signal. 2) The breaths are easier to identify in the PPG respiratory signal than in the impedance signal (subjective measure). CONCLUSIONS: Electrode and motion artefacts seem to disturb the ECG signals and, particularly, the impedance signals. During periods of high quality ECG and impedance signals, the new optical device produces signals of equal quality to these traditional methods, and is in some cases even better. The new device is non-invasive and has a small optical probe. These factors indicate further advantages of the photoplethysmographic method.

Electric Impedance↗

Design and fabrication of a silica on silicon integrated optical biochip as a fluorescence microarray platform.

Previous research into the use of Flame Hydrolysis Deposition (FHD) of glasses in integrated optics has focused on the successful commercial exploitation of low cost optical devices within the field of telecommunications and optoelectronics. Recently we have sought to apply these fabrication technologies to the development of optical biochips, utilising their ability to be integrated with microfluidics as a 'Lab-on-a-chip' platform. In this paper, we carry this development forward by seeking to create a microarray of integrated optical sensing elements, addressed using a glass-polymer hybrid technology in which poly(dimethylsiloxane), PDMS, is used as an elastomeric packaging over-layer. In particular, we describe the wide range of modelling and microfabrication processes required for the successful manufacture, integration and packaging of such arrays. The integration of both optical and fluidic circuits in this device avoids precise alignment requirements and results in a compact, robust and reliable device. Finally, in this paper, we describe the implementation of a pumping system for delivering small amounts of fluid across the array together with an optical signal treatment.

Carbocyanines↗

Detection of platelet aggregates with a particle counting method using light scattering.

A novel method to detect platelet aggregation by means of the particle counting technique using light scattering has been developed. An optical device designed to focus on a limited area of platelet-rich plasma measured the intensity of light scattered by particles passing through the area, minimizing multiple light scattering. The use of polystyrene spheres of different diameters confirmed that the light scattering intensity increases in proportion to the particle size in a suspension. Platelet activation induced by various agonists resulted in light scattering of higher intensities, which correlated well with the number and size of aggregates as observed under a microscope. These findings confirmed that the intensity of light scattering detected by the new device provides information on the number and size of aggregates in a suspension. The new method was compared with conventional platelet aggregometry using overall light scattering or changes in light transmission (optical density). The new device appeared to be particularly sensitive to small aggregates such as those formed in platelet activation induced by low concentrations of agonists. Furthermore, the new method has an advantage over the conventional aggregometry, in that it allows the aggregate size distribution and the extent of aggregation to be estimated.

Adenosine Diphosphate↗