Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Opossums”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources
PubMed1998

Neurogenesis and migration of receptor neurons in the vomeronasal sensory epithelium in the opossum, Monodelphis domestica.

The sensory epithelium of the vomeronasal organ (VNO) contains primary chemosensory receptor neurons that project to the accessory olfactory bulb (AOB). In the present study, neurogenesis and cell migration in the sensory epithelium of the VNO were analyzed in opossums (Monodelphis domestica) by using bromodeoxyuridine (BrdU) labeling. 1) In the VNO of normal adult opossums, BrdU labeled a small number of cells localized in the basal region of the sensory epithelium. After 1 or 2 weeks of survival, the labeled cells appeared in the receptor cell layers and became receptor neurons, as indicated by coexpression of the G proteins G(i alpha2) or G(o alpha). 2) In the VNO in which the receptor neurons had been destroyed by removing the AOB, the number of BrdU-labeled cells in the reconstituting sensory epithelium was greatly increased compared with that in the intact VNO. The labeled cells were also located in the basal region of the sensory epithelium. 3) In the developing VNO (at postnatal day 10), more cells in the basal region of the sensory epithelium were labeled than in the adult VNO, indicating rapid cell proliferation; and there appeared to be more labeled cells in the basal region near the margins of the sensory epithelium where it meets the nonsensory epithelium. These observations demonstrate that, in the opossum VNO, there is a population of proliferating cells in the basal region close to the basal lamina in the sensory epithelium. The newly generated neurons in the basal region migrate vertically into the receptor cell layer.

Animals↗
PubMed1976

Type II pulmonary epithelial cells of the newborn opossum lung.

The air chambers of the newborn opossum are lined by a respiratory epithelium which appears similar to that lining alveoli of other species. The type II pulmonary epithelial cell is cuboidal in shape, shows apical microvilli, several Golgi complexes, lipid droplets, and numerous cytosomes. The cytosomes contain osmiophilic lamellae and are similar in appearance to whorls of osmiophilic material lying free within the alveolar lumina and on the surface of the respiratory lining epithelium. In the newborn opossum lung, examination of the air chambers with the scanning electron microscope permits the observation of several type II pulmonary epithelial cells in a single field. The potential use of the air chamber of the newborn opossum as an experimental model is suggested.

Animals↗
PubMed1991

Ipsilaterally projecting rubrospinal neurons in adult and developing opossums.

We have combined injections of Fast Blue with lesions of the rubrospinal tract rostral and contralateral to them to determine if an ipsilateral rubrospinal projection exists in adult or developing opossums and, if so, to characterize the neurons giving rise to it. Although the results indicate that some rubral neurons project ipsilaterally, they are very few in number. Using quantitative and image analysis techniques, we have shown that 0.6% of the rubral neurons that project to the lumbar cord in adult opossums do so ipsilaterally and that such neurons are comparable in location and size to those that project contralaterally. Similar results were obtained in developing opossums. Our results are discussed in light of rubrospinal development and ongoing experiments related to rubrospinal plasticity.

Amidines↗
PubMed1995

Cytoarchitecture of the substantia nigra pars lateralis in the opossum (Didelphis virginiana): a correlated light and electron microscopic study.

BACKGROUND: The substantia nigra has been divided into three subdivisions. However, the cytoarchitecture of one of these subdivisions, the pars lateralis (SNl), has not been previously examined in detail at the light and electron microscopic levels in any species. In the adult opossum, the three nigral subdivisions can be easily distinguished as distinct, rostrocaudally oriented cell groups separated by neuron-free zones. Thus it was possible to determine the boundaries of the SNl unambiguously. This report covers the results of an examination of the morphology and organization of the SNl in the opossum. METHODS: Material from 13 opossums was used for this study. Eight of the animals had been previously stained for Nissl substance (n = 4) or impregnated by the Golgi technique (n = 4). The remaining five animals were prepared for electron microscopic studies using standard procedures. RESULTS: Two cell types were identified on the basis of morphological differences, small and medium-large neurons. Small neurons (10-18 microns long axis) have large nuclei with moderate amounts of heterochromatin and a thin rim of cytoplasm. They have long (up to 500 microns), spine-free dendrites. Medium-large neurons (18-54 microns long axis) have rounded nuclei with electron-lucent nucleoplasm. Few indentations of the nuclear envelope were observed. The surrounding cytoplasm has dense arrays of organelles. Nissl bodies are particularly prominent in the form of pyramids with their bases at juxtanuclear positions and their apices directed toward emerging dendrites. Dendrites of medium-large neurons are long (some > 1 mm in length), are primarily oriented in the frontal plane, and extend along the dorsal surface of or into the cerebral peduncle. Some cells have dendrites that are moderately spinous, whereas other neurons possess sparsely spinous dendrites. Relatively few synaptic profiles are observed to contact somata and proximal dendrites. CONCLUSION: This report provides added morphological support for the idea that the SNl is a distinct subdivision of the substantia nigra, a distinction previously made on the basis of the physiologically characterized relationship between the lateral substantia nigra and orienting behaviors and seizure-related function.

Animals↗
PubMed1975

The inferior olivary nucleus of the opossum (Didelphis marsupialis virginiana), its organization and connections.

Although the inferior olivary nucleus of the opossum is small, sections stained either for Nissl substance, normal axons or cholinesterase activity reveal distinct medial, dorsal and principal nuclei. The medial nucleus contains three major subdivisions (labelled a, b, c after Bowman and Sladek, '73) and a group of neurons which is comparable to the cap of Kooy. In contrast to the cat and monkey, the major portion of the "medial" nucleus (subgroup a) lies lateral to the principal nucleus in rostral sections. The dorsal nucleus can also be subdivided, as can the principal nucleus which contains distinct dorsal and ventral lamellae. A small area is identified which based on position and connections may conform to the dorsal medial cell group. The experimental portion of the study provides evidence for an olivary projection from the motor-sensory cortex and a massive input from the midbrain (red nucleus, pretectum, midbrain tegmentum). In addition, the opossum inferior olive receives fibers from the deep cerebellar nuclei (cerebellar feedback loops), the spinal cord and the dorsal column nuclei. Of particular interest is the finding that fibers from the nucleus cuneatus and nucleus gracilis have distinctly different olivary targets and that those from the nucleus gracilis, but not the cuneate nucleus, overlap (in part, at least) with the direct spinal fibers. Other examples of overlapping fields of terminal degeneration are present and are discussed. In general our results reveal that although certain relationships between the nuclear divisions are different, the opossum olive conforms well to that of placental mammals and provides a basic mammalian model for future experimental electron microscopic and physiological studies.

Animals↗
PubMed1977

The vestibular complex of the American opossum didelphis virginiana. II. Afferent and efferent connections.

We have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. Apart from the primary input described in Part I of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. Axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g., the dorsal part of the lateral vestibular nucleus, the dorsolateral margin of the inferior vestibular nucleus as well as cell groups comparable to "f" and "x." In contrast, fastigial fibers show considerable overlap with primary vestibular input, particularly in the ventral part of the lateral nucleus, the central part of the inferior nucleus and the medial nucleus. Axons of fastigial origin also distribute to the superior vestibular nucleus, to subnuclei "f" and "x" and to the parasolitary region. Although spinal fibers are diffuse within the main vestibular nuclei, they ramify quite densely within subnucleus "x." Most of the spinovestibular projection appears to arise in the cervical spinal cord of the opossum. Ipsilateral connections from the interstitial nucleus of Cajal and surrounding areas end predominantly, but not exclusively, in the medial vestibular nucleus. A crossed midbrain projection has been verified from the red nucleus to cell group "x" and the lateral part of the inferior nucleus, as well as to an area possibly comparable to cell group "z," as described for the cat. In Part I of our study we have shown that the major targets of primary vestibular fibers are the central part of the superior nucleus, a portion of the parabrachial complex possibly comparable to subnucleus "y"," the ventral part of the lateral nucleus and the medial nucleus. All of these primary zones give rise to fibers supplying extraocular nuclei and surrounding areas (present study). The ascending midbrain fibers from the superior nucleus end mainly ipsilaterally, whereas those from the putative subnucleus "y" and the medial vestibular nucleus distribute contralaterally for the most part. Although the dorsal part of the lateral vestibular nucleus has no primary vestibular input, it does receive a major projection from the cerebellar cortex. This same region of the lateral nucleus projects to the spinal cord, but not to extraocular nuclei. The ventral part of the lateral nucleus, and perhaps the medial nucleus, also relay to the spinal cord. Additional projections from all vestibular nuclei to the reticular formation provide indirect routes through which the vestibular nuclei can potentially effect multiple systems, including those innervating the spinal cord. Finally, commissural vestibular connections of the opossum are shown to arise within all four major nuclei.

Afferent Pathways↗
PubMed1980

Early development of the inferior olivary complex in pouch young opossums. II. An electron microscopic study.

At birth the inferior olivary complex (IOC) is not present in the caudal ventro-medial brainstem of the opossum. In the 3-7-day-old animal (15-19 days post-conception), this same region does contain neurons of the developing IOC. The immature neurons are characterized by large, centrally placed nuclei surrounded by a thin rim of cytoplasm. The neuropil contains numerous small-diameter profiles which contain bundles of filaments and scattered microtubules. Occasional synaptic endings, containing round clear vesicles, contact large, flocculent profiles. By 10-14 days of age, the olivary complex begins to separate into individual nuclei; however, the olivary cell bodies and the surrounding neuropil exhibit many of the same features as in the 3-7-day-old opossums. In opossums 21-25 days old, there is an increase in varicosities and irregular contours along many of the dendritic shafts. Furthermore, synaptic terminals, possessing round clear vesicles, now contact the soma, dendritic shafts, dendritic varicosities, spines, and large, flocculent profiles. Terminals containing pleomorphic vesicles or a mixture of clear and large granular vesicles are presynaptic only to dendritic spines or large, flocculent profiles. Neuroglial cell bodies have been identified at all ages examined. It is not until days 65-68 that pre- and postsynaptic elements are organized into synaptic clusters (glomeruli), which are typical of the adult. Spiny appendages and small-diameter dendrites comprise the central core of the clusters which are surrounded by synaptic endings containing a variety of vesicle types. Thus it would appear that subsequent to their initial arrival (day 16-17), the synaptic relationships of cerebellar and midbrain afferents are modified to reflect their adult configuration by days 65-68. This extended period of development (postnatal days 3-68) for the olivary complex provides a good model for assessing the effects of experimental manipulations.

Animals↗
PubMed1984

Cerebellar cortical efferent fibers in the North American opossum, Didelphis virginiana. II. The posterior vermis.

The projection pattern of corticonuclear and corticovestibular fibers from vermis lobules VI-X of the North American opossum, Didelphis virginiana, was studied using a modification of the Fink and Heimer ('67) technique. The evidence suggests that corticovestibular projections in opossum are ipsilateral and exit the cerebellum via the juxtarestiform body. Lobules VI and VII contribute few, if any, corticovestibular projections. Corticovestibular fibers from lobule VIII are sparse to moderate and those from lobules IX and X are numerous. The main targets of corticovestibular fibers are the superior and spinal vestibular nuclei with some input to the medial vestibular nucleus, particularly from lobules IX and X. Although degenerated axons course through the lateral vestibular nucleus, they do not appear to terminate therein. Corticonuclear fibers from lobules VI-X are ipsilateral and terminate in the caudal and caudoventral one-third of the medial cerebellar nucleus (NM). Fibers from lateral areas of some vermal lobules appear to enter contiguous parts of the immediately adjacent posterior interposed nucleus. Although each lobule projects into a specific area of caudal and caudoventral NM when the terminal fields for lobules VI-X are superimposed, it is apparent that they are largely coextensive. This is in contrast to the pattern seen in projections from the anterior vermis. The results further indicate that zones A and B are present in lobules VI-X of opossum.

Animals↗
PubMed1988

Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in the brain and nervus terminalis of the adult and early neonatal gray short-tailed opossum (Monodelphis domestica).

Luteinizing hormone-releasing hormone (LHRH) was detected by immunocytochemical procedures in cells and fibers in the brain and in the nervus terminalis of the adult and neonatal gray, short-tailed opossum (Monodelphis domestica). As in several species of eutherian mammals, LHRH-immunoreactive cells and fibers were seen in the medial septal nuclei, nucleus, and tract of the diagonal band and olfactory tubercle. Surprisingly, few LHRH-immunoreactive cells were found in the hypothalamus or in the preoptic area in either the adult or neonate. LHRH-immunoreactive fibers were seen in these regions and were numerous in the median eminence. The nervus terminalis in the gray opossum showed several distinctive characteristics. Immunoreactive and nonreactive cells, in ganglia along the peripheral and intracranial course of this nerve, were accompanied by thick fascicles of LHRH fibers and frequently lay adjacent to blood vessels. No LHRH-immunoreactive branches of the nervus terminalis were found in contact with the vomeronasal nerves as they traversed the medial surfaces of the main olfactory bulbs en route to the accessory olfactory bulbs. The LHRH-immunoreactive fibers in the central roots of the nervus terminalis formed a compact bundle on either side of midline, which coursed obliquely from clusters of ganglion cells on the ventromedial surface of the olfactory bulbs into the septum. Traced through serial sagittal sections, LHRH-immunoreactive fibers, in continuity with those in the triangular nucleus of the septum, ran down and around the rostral face of the anterior commissure and fanned out into the medial preoptic area. As previously observed in the fetal guinea pig and rat, LHRH was detected in ganglion cells of the nervus terminalis of the newborn gray opossum preceding its detection in any other area of the brain.

Aging↗
PubMed1990

Morphological organization of thalamocortical relay cells in the dorsal lateral geniculate nucleus of the North American opossum.

The morphology of relay cells in the dorsal lateral geniculate nucleus of the North American opossum was studied by using both Golgi-Cox material and cells stained from retrograde transport of horseradish peroxidase. In general, soma sizes were largest in the part of the nucleus representing the central retina and decreased from the middle third of the nucleus to the anterior to posterior poles. Relay cells labeled with horseradish peroxidase were found to constitute approximately 90% of the dorsal lateral geniculate nucleus cells and have larger soma diameters than most unlabeled cells. From morphometric analysis of several structural characteristics, three classes of relay cells were identified in both Golgi-Cox and horseradish peroxidase material. Type 1 cells, the predominant class, exhibited radially arranged primary dendritic fields, symmetrically organized relative to projection lines. Type 2 cells had relatively few primary dendrites, and complex dendritic fields that were oriented parallel to projection lines. Least numerous were Type 3 cells, which were characterized by relatively sparse dendritic fields oriented perpendicular to projection lines. An additional class of neuron, Type 4 cells, with small somata and sparse dendritic branching, was found only in Golgi-Cox material. Cells with Type 4 dendritic morphology were not found with retrograde horseradish peroxidase labeling and may represent interneurons. The classification of morphologically characterized cells in the opossum dorsal lateral geniculate nucleus was evaluated quantitatively with multivariate discriminant analysis. The classes are compared to physiologically identified Y-, X-, and W-like relay cells in the opossum and to relay cell classes in other species.

Animals↗
PubMed1991

Organization of midbrain catecholamine-containing nuclei and their projections to the striatum in the North American opossum, Didelphis virginiana.

Presumptive catecholamine (CA) neurons in the opossum midbrain were identified by tyrosine hydroxylase immunohistochemistry. In the midline, small to moderate number of CA cells were present in the rostral third of the nucleus raphe dorsalis and throughout the nucleus linearis. Ventrolaterally, such cells were observed in the deep tegmental reticular formation, in all subnuclei of the ventral tegmental area, and in the three subdivisions of the substantia nigra. The CA cells in these areas conform to the dopamine cell groups, A8, A9, and A10 as described in the rat. In several areas there appeared to be no separation between the CA neurons belonging to cytoarchitecturally different nuclei. In order to determine which CA neurons gave rise to striatal projections, the neostriatum was injected with True Blue (TB), and sections through the midbrain were processed for tyrosine hydroxylase (TH) and visualized by immunofluorescence. Neurons containing both TB and TH were observed in each of the CA cell groups mentioned above. The distribution of these cells confirmed organizational features that may be unique to the opossum's substantia nigra. In addition, different patterns of labeling resulted from caudate versus putamen injections, suggesting a rudimentary medial to lateral topography in the organization of nigrostriatal projections. Although our results suggest that the organization of midbrain CA neurons in the opossum is similar to that in placental mammals, it is clear that differences exist.

Animals↗
PubMed1984

Studies on the energy metabolism of opossum (Didelphis Virginiana) erythrocytes. I. Utilization of carbohydrates and purine nucleosides.

Opossum erythrocytes filtered through cellulose columns were used to estimate their permeability to D-glucose and optimum inorganic phosphate requirement for D-glucose utilization at pH 7.4 and 8.1. D-Glucose readily penetrated opossum red cells; there was no measurable difference whether plasma or electrolyte solution served as the suspending medium. Optimum extracellular inorganic phosphate concentration for glucose utilization as indicated by red cell lactate production was pH-dependent, with a sharp optimum of 30 mmol/liter at pH 8.1. Whereas glucose, fructose, mannose, dihydroxyacetone, adenosine, and inosine were readily utilized at pH 7.4 and Pi 30 mmol/liter as shown by net lactate and ATP production by the red cells, galactose and ribose as substrates were not metabolized. In electrolyte, Pi 30 mmol/liter, and pH 7.4 glucose utilization by opossum red cells averaged 3.5 mumol, at pH 8.1, 9.5 mumol/ml cells/hr were utilized. Red cells suspended in leukocyte-free plasma utilized D-glucose at a rate of 3.0 mumol/ml/hr at pH 7.5. Seven percent of D-glucose flowed through the pentose phosphate pathway; this rate increased 11-fold by methylene blue stimulation. The amount of D-glucose recycled through the pentose phosphate pathway increased 300-fold in the presence of the redox dye.

Adenosine Triphosphate↗
PubMed1993

Gestation and placentation in two New World opossums: Didelphis virginiana and Monodelphis domestica.

Estrus, gestation, and placentation were studied in the Virginia opossum (Didelphis virginiana) and the gray short-tailed oppossum (Monodelphis domestica), representatives of the prototypic, New World marsupial family Didelphidae. Didelphis is a large (3,000 g), pouched opossum that exhibits a 28-day estrous cycle, spontaneous estrus and ovulation, and a high ovulation rate (60 ova per cycle). Monodelphis is a small (100 g), pouchless opossum with male-induced estrus and ovulation and a moderate ovulation rate (12 ova per cycle). Implantation occurs on day 10 of the 13-day gestation period in Didelphis and on day 12 of the 15-day gestation in Monodelphis. Gestation and placentation, including the ultrastructural features of trophoblastic apposition to the uterine mucosa, are very similar in Didelphis and Monodelphis. Differences, such as a larger increase in uterine volume and a more highly folded endometrium following implantation in Monodelphis are minor. They appear to be related to the smaller maternal body size and the relatively larger uterine load of embryos in Monodelphis. Because body size is one of the major variables among New World marsupials, the descriptions of gestation and placentation presented here for Didelphis and Monodelphis, provide a basis for comparison in future studies of didelphid marsupials.

Animals↗
PubMed1995

Lectin histochemical identification of carbohydrate moieties in opossum chemosensory systems during development, with special emphasis on VVA-identified subdivisions in the accessory olfactory bulb.

Lectins, sugar-binding molecules of nonimmune origin, were used in this study to describe the development of the main olfactory and vomeronasal systems in the Brazilian gray short-tailed opossum, Monodelphis domestica. A battery of seven lectins of the N-acetylgalactosamine/galactose-binding group was used. Of the seven lectins, only two, Vicia villosa agglutinin (VVA) and Griffonia (Bandeiraea) simplicifolia lectin I-isolectin B4 (GS I-B4), were specific to the vomeronasal system. The other five lectins recognized carbohydrates in both chemosensory systems, although the binding was more intense in the accessory olfactory system. Furthermore, whereas six of the lectins stained the adult opossum accessory olfactory bulb (AOB) homogeneously, the VVA lectin distinguished two regions of the AOB. Similar to the expression of olfactory marker protein (OMP) (Shnayder et al. [1993] Neuroreport 5:193-196), the rostral half of the AOB stained much darker with VVA than the caudal half, and the onset of the restricted pattern of staining at age 45 days also coincided. We conclude that 1) GS I-B4 and VVA recognize cell surface carbohydrate moieties specific to the vomeronasal, but not to the main olfactory, system, and 2) the carbohydrate moiety that is recognized by the VVA lectin, presumably terminal N-acetyl-galactosamine, is both temporally and spatially restricted in the opossum AOB. These results are discussed in the framework of other known spatially restricted molecules of the two major nasal chemosensory systems.

Acetylgalactosamine↗
PubMed1992

Non 5-HT1A/5-HT1C [3H]5-HT binding sites in the hamster, opossum, and rabbit brain show similar regional distribution but different sensitivity to beta-adrenoceptor antagonists.

We have used receptor autoradiography to investigate the distribution and pharmacological profile of non 5-HT1A/5-HT1C[3H]5-hydroxytryptamine binding sites in the brain of rabbits, hamsters and opossums. These data were compared to those found under similar conditions in the brain of rats and guinea pigs, species which are known to possess 5-HT1B and 5-HT1D receptors, respectively. In the presence of 100 nM 8-OH-DPAT and mesulergine, the regional distribution of [3H]5-hydroxytryptamine binding sites was very similar in the brain of all species investigated; densest labelling was observed in the globus pallidus, substantia nigra and superior colliculus. In all species, 5-carboxamidotryptamine competed for the labelled sites in a biphasic manner and metergoline displayed a subnanomolar affinity. In contrast, the beta-adrenoceptor blocking agents (-)propranolol, (-)pindolol, and (+/-)SDZ 21009 were potent displacers only in the rat, hamster and opossum brains. These data indicate that non 5-HT1A/5-HT1C[3H]5-HT binding sites display a high affinity for these agents in a particular rodent suborder as well as in opossum, a phylogenetically unrelated species.

8-Hydroxy-2-(di-n-propylamino)tetralin↗
PubMed1998

Adult opossums (Didelphis virginiana) demonstrate near normal locomotion after spinal cord transection as neonates.

When the thoracic spinal cord of the North American opossum (Didelphis virginiana) is transected on postnatal day (PD) 5, the site of injury becomes bridged by histologically recognizable spinal cord and axons which form major long tracts grow through the lesion. In the present study we asked whether opossums lesioned on PD5 have normal use of the hindlimbs as adults and, if so, whether that use is dependent upon axons which grow through the lesion site. The thoracic spinal cord was transected on PD5 and 6 months later, hindlimb function was evaluated using the Basso, Beattie, and Bresnahan (BBB) locomotor scale. All animals supported their weight with the hindlimbs and used their hindlimbs normally during overground locomotion. In some cases, the spinal cord was retransected at the original lesion site or just caudal to it 6 months after the original transection and paralysis of the hindlimbs ensued. Surprisingly, however, these animals gradually recovered some ability to support their weight and to step with the hindlimbs. Similar recovery was not seen in animals transected only as adults. In order to verify that descending axons which grew through the lesion during development were still present in the adult animal, opossums subjected to transection of the thoracic cord on PD5 were reoperated and Fast blue was injected several segments caudal to the lesion. In all cases, neurons were labeled rostral to the lesion in each of the spinal and supraspinal nuclei labeled by comparable injections in unlesioned, age-matched controls. The results of orthograde tracing studies indicated that axons which grew through the lesion innervated areas that were appropriate for them.

Age Factors↗
PubMed1997

Male gray short-tailed opossums (Monodelphis domestica) receive penile intromissions when treated with estrogen and progesterone in adulthood.

Following treatment with estradiol and progesterone, gonadectomized male as well as female gray opossums received penile intromissions from intact stimulus males. Intromission was possible in male gray opossums because, like marsupials of both sexes, they possess a single cloaca-like anogenital opening. All subjects that allowed intromission showed anogenital dragging just prior to intromission. While intromission latency was similar in tests involving male and female subjects, total intromission duration was longer in tests involving male subjects than in tests involving female subjects, and sex locks were seen only in tests involving female subjects. These findings are discussed with respect to the potential usefulness of gray opossums for studying the effects of peripheral vs central factors on the display of sex differences in behavior.

Animals↗
PubMed1991

Patterns of myelination in the opossum superior colliculus with additional reference to the optic tract.

Patterns of myelination have been studied in the optic tract and the superior colliculus (SC), with special reference to the optic layer, in the opossum Didelphis marsupialis. Myelination in the optic tract starts far in precedence of eye opening and follows a rostrocaudal gradient. Myelination in the SC presents the following features: it proceeds according to a general inside-out pattern and follows both rostro-caudal and latero-medial gradients in the optic layer, and it accelerates in the SC optic layer soon after systematic exposure to visual input. The data presented here, together with other available information, suggest that myelination in the opossum optic tract starts in parallel with the stabilization in the number of optic fibers, and advances in the rostro-caudal mode common to most eutherian mammals, and also that myelogenesis in the SC neither correlates necessarily with, nor recapitulates, the sequence of acquisition of GFAP-positive astrocytes in a given set of layers. Changes in the rate of myelination in the optic layer after exposure to visual input are regionally-selective, and seem compatible with the recruitment of thin axons into the myelogenetic cycle rather than with the thickening of pre-existing myelin sheaths. It is concluded that the SC is a favorable structure for the study of the differentiation of glial cells, particularly in species with an extended time course of maturation such as the opossum.

Animals↗