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PubMed2005

Particulate-biofilm, expanded-bed technology for high-rate, low-cost wastewater treatment: nitrification.

The performance of a particulate-biofilm, expanded-bed process for nitrification of activated sludge final effluent (ASFE) is reported for a plant receiving mixed industrial and domestic wastewater. The support material for the particulate-biofilms was glassy coke, to which the nitrifying bacteria attached and formed a highly active biofilm. An average nitrification rate of 1.7+/-0.6 kg m(expanded bed)(-3)d(-1) was recorded during operation of the bioreactor, which had a hydraulic residence time of 15 min. On average, the ASFE contained 12.6+/-3.7 g m(-3) NH3-N, which was reduced to 2.6+/-3.3 g m(-3) NH3-N. Furthermore, transfer of 10-12% of the oxygen in air was achieved using counter-current aeration. This investigation has demonstrated that a high rate of nitrification can be achieved with a particulate-biofilm, expanded-bed process. It has also demonstrated that the process can operate without backwashing and still remove particulate material from the ASFE feed.

Air↗
PubMed2005

Effect of heat recovery from raw wastewater on nitrification and nitrogen removal in activated sludge plants.

By recovery of heat from the raw wastewater in the sewer system, the influent temperature of a wastewater treatment plant (WWTP) is reduced. This can have a negative effect on nitrification in the WWTP, since this process strongly depends on temperature. The analysis of the temperature regime in the WWTP of Zurich, Switzerland, revealed that in the cold season, the effluent temperature is about 0.7 degrees C higher than the influent temperature and that nitrification is not affected by a decrease of the influent wastewater temperature lasting for a couple of hours only, but is significantly affected by a longer lasting temperature decrease. Three diagrams were developed with a steady-state model, from which the consequences of a permanent temperature decrease on the nitrification safety factor, aerobic sludge retention time and total nitrogen removal can be evaluated. Using simulations with a dynamic model, calibrated for the Zurich WWTP, a quantitative relationship between the wastewater temperature and the ammonium effluent concentration was established. This relationship can, in combination with measured effluent concentrations of an existing WWTP, be used to predict the increase of the ammonium effluent concentration in this plant resulting from a permanent decrease of the wastewater influent temperature.

Hot Temperature↗
PubMed2006

Effective partial nitrification to nitrite by down-flow hanging sponge reactor under limited oxygen condition.

Combining the processes of partial nitrification and anaerobic ammonium oxidation (ANAMMOX) is an attractive wastewater-treatment technology for nitrogen removal. In this study we investigated partial nitrification by implementing a closed down-flow hanging sponge (DHS) reactor operated at controlled oxygen concentrations. Basic concept of DHS process is similar to that of trickling filter, in which oxygen concentration can be easily manipulated by controlling airflow to the reactor. The closed reactor was fed with artificial wastewater containing NH(4)Cl and operated with an HRT of 1.5h at 30 degrees C. Oxygen inside the reactor was maintained below 3% (1.2mgDO x L(-1)) (DO-dissolved oxygen) except during the startup periods. Five months of continuous operation showed that there was a strong relationship between oxygen concentration and nitrite production. The ratio of nitrite produced relative to ammonium oxidized increased by decreasing oxygen concentration. Partial nitrification was satisfactorily accomplished under oxygen limitation at around 0.5% in the gas phase (0.2mgDOL(-1)). The system showed a high ammonium-removal rate, at a maximum of 1.46kg NH(4)(+)-Nm(-3)day(-1), even at limited oxygen concentration. We also found that oxygen concentration played an important role in the production of nitrous oxide, which increased with decreasing oxygen concentration.

Air Movements↗
PubMed2001

The effect of decreased ambient temperature on the biological nitrification and denitrification of a high ammonia landfill leachate.

This research investigated the nitrogen removal capabilities of a pre- and post-denitrification process, when treating sanitary landfill leachate containing an ammonia concentration of over 2200 mg N/L, at operating temperatures down to 10 degrees C. The treatment system employed is generally known as a 4-Stage Bardenpho process. Two parallel systems were operated throughout the study; each system had an aerobic solids retention time of 20 days and an external recycle ratio (return activated sludge) of about 3:1. During the entire experimental period, one system used an internal recycle ratio of about 4:1, while the other system used one of 3:1. Methanol was used as supplementary carbon source for denitrification. When operating temperature was decreased from 20 to 17 degrees C, an approximate 15% decrease in denitrification was immediately experienced within both systems, with no noticeable affect on nitrification. This was an unexpected result. Subsequently, the temperature was further decreased to 14 degrees C, and finally to 10 degrees C. With the temperature at 14 degrees C, aerobic wasting was also stopped and methanol loading was progressively decreased to match actual denitrification requirements. At 10 degrees C, both systems suffered major nitrification and denitrification inhibition. Changes in operating parameters, such as a decrease in influent ammonia and methanol loading, as well as an increase in ambient temperatures, from 10 to 15 degrees C, did not significantly improve overall system performance, within a reasonable time frame. Both denitrification and nitrification problems were mainly attributed to decreased ambient temperature, although other possible factors were noted; at project completion, about 50% of the incoming ammonia was being removed across the system.

Aerobiosis↗
PubMed2001

Application of the 15N technique to determine the contributions of nitrification and denitrification to the flux of nitrous oxide from aerated pig slurry.

In order to elucidate and quantify nitrogen transformations occurring during aerobic treatment of pig slurry, two laboratory experiments were carried out with contrasting levels of aeration, high level (experiment 1) and low level (experiment 2) of aeration. During these experiments, after reaching steady-state conditions, a single pulse of NO3(-)-15N tracer was added directly to the reactor. When nitrification conditions were prevailing (experiment 1), no transformation of added NO3(-)-15N occurred (98.7% 15N-recovery as nitrates in the liquid slurry). Moreover, the unlabelled nitrous oxide (N2O) measured (7.4% of the total nitrogen content of the raw slurry) strongly demonstrated that the source of N2O emitted was nitrification. During simultaneous nitrification and denitrification conditions (experiment 2), the added NO3(-)-15N was found in gaseous nitrogen forms (N2O-15N: 27%; N2-15N: 18%) and in the liquid (TN-15N: 54%). From this result, it was evident that N2O was mainly emitted by denitrification process. Using the decrease of the isotopic excess of the NO3(-)-15N we calculated that 92% of NO2(-)-N was directly denitrified (as gaseous forms) without any previous oxidation to nitrate.

Aerobiosis↗
PubMed2002

Occurrence of nitrifying bacteria and nitrification in Finnish drinking water distribution systems.

Microbiological nitrification process may lead to chemical, microbiological and technical problems in drinking water distribution systems. Nitrification activity is regulated by several physical, and chemical, and operational factors. However, the factors affecting nitrification in the distribution systems in boreal region, having its specific environmental characteristics, are poorly known. We studied the occurrence and activity of nitrifying bacteria in 15 drinking water networks distributing water with very different origin and treatment practices. The waters included chloraminated surface water, chlorinated surface water, and non-disinfected groundwater. The networks were located in eight towns in different parts of Finland. Our results showed that nitrifying bacteria are common in boreal drinking water distribution systems despite their low temperature. Surprisingly high numbers and activities of nitrifiers were detected in pipeline sediment samples. The numbers of ammonia-oxidizing bacteria and their oxidation potentials were highest in chloraminated drinking water delivering networks, whereas the nitrite-oxidizing bacteria were present in the greatest numbers in those networks that used non-disinfected groundwater. The occurrence of nitrifying bacteria in drinking water samples correlated positively with the numbers of heterotrophic bacteria and turbidity, and negatively with the content of total chlorine. Although nitrifying bacteria grew well in drinking water distribution systems, the problems with nitrite accumulation are rare in Finland.

Ammonia↗
PubMed2001

Salt inhibition kinetics in nitrification of synthetic saline wastewater.

Nitrification kinetics of synthetic saline wastewater was investigated by using an activated sludge unit. Initial experiments were performed with salt-free wastewater to obtain baseline information. Experiments with 3% salt concentration were performed at different sludge ages in order to investigate the system performance and salt inhibition effects on kinetic constants. Minimum sludge age required for complete nitrification increased from 12 days for salt-free wastewater to 25 days for 3% salt content. Salt inhibition was non-competitive type affecting both the maximum rate and the saturation constants. Inhibition constants were determined by using the experimental data as K(T1) = 200 g/liter and K(T2) = 7.4 g/liter. Further experiments were performed with salt concentrations between 0-5% to quantify variation of the rate and extent of COD removal with salt concentration. The rate and extent of nitrification decreased approximately 20% with 5% salt as compared to salt-free wastewater.

Journal Article↗
PubMed2003

Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor.

Nitrogen and carbon components in domestic modified wastewater were completely removed by simultaneous nitrification and denitrification using a membrane-aerated biofilm reactor where biofilm was fixed on a hollow-fiber membrane. To measure the spatial distribution of pH, ammonium and nitrate ions and to observe microbes inside the biofilm fixed on the membrane, microelectrodes and the fluorescence in situ hybridization (FISH) method were applied. Due to plug flow in the vertical direction (from the bottom to the top of the reactor), ammonium nitrogen was gradually removed and negligible nitrate nitrogen was detected throughout the reactor. FISH revealed that ammonia-oxidizing bacteria were mainly distributed inside the biofilm and other bacteria, which included denitrifying bacteria, were mainly distributed outside the biofilm and over the suspended sludge. In order to characterize bacterial activity in the vertical direction of the reactor, nitrification rates at lower, central and upper points were calculated using microelectrode data. The nitrification rate at the lower point was 7 and 125 times higher than those at the central and upper points, respectively. These results show that the removal of carbon and nitrogen compounds was accomplished efficiently by using various kinds of bacteria distributed vertically and horizontally in a single reactor.

Bacteria, Aerobic↗
PubMed2003

Simultaneous nitrification-denitrification in slow sand filters.

While the ability of slow sand filters to remove total suspended solids (SS), turbidity, and organics from wastewaters is well known, this study has demonstrated that they can also achieve simultaneous nitrification-denitrification, producing effluent total Kjedahl nitrogen (TKN) and total nitrogen (TN) concentrations as low as 0.6 and 1.5mg/l, respectively, utilizing particulate and slowly biodegradable COD in the process. The impact of filtration rates in the range of 0.15-0.38m/h, filter depth of 0.5-1.5m, and sand size 0.3-0.5mm on nitrogen removal processes at temperatures of 10-39 degrees C was assessed. Nitrification efficiency, denitrification efficiency, and total nitrogen removal efficiency correlated well with filtration rate and sand size only, with all three parameters inversely proportional to the square root of the aforementioned two process variables. Nitrification exhibited the most sensitivity to filtration rate and sand size. The filters produced effluent with turbidities of 0.1-0.5 NTU, SS concentrations of 3-6mg/l in the fine sand and 6-9mg/l in the coarse sand. Effluent BOD(5) and COD concentrations were mostly in the 0.8-2.6 and 15-34mg/l range, respectively.

Filtration↗
PubMed2003

Medicinal and aromatic plant materials as nitrification inhibitors for augmenting yield and nitrogen uptake of Japanese mint (Mentha arvensis L. Var. Piperascens).

Pot experiments were conducted to evaluate the relative performance of medicinal and aromatic plant materials and dicyandiamide (DCD) as nitrification inhibitors to regulate transformation of N from urea. Their effect on the efficiencies of use of N by Japanese mint (Mentha arvensis cv. Hy 77) was tested. Urea was coated with these materials viz., Mentha spicata, Artemisia annua or DCD at the rate of 5% (w/w) of fertilizer urea using an appropriate coating technique. Nimin (tetranortriterpenoids, an ethanol extract of neem (Azadirachta indica Juss) coating was done at the rate of 1% w/w of urea. Fertilizer nitrogen was applied at 100 and 200 mg kg(-1) soil. These natural coating materials significantly increased the herb and essential oil yields of the crop at both rates of fertilizer nitrogen compared to urea alone and were found to be as effective as DCD in retarding NO3- formation in soil. Herb yield increased by 6-81% when compared to uncoated urea. The increase in essential oil yield ranged between 3% and 68% due to coating. The effectiveness of the nitrification-inhibitor--coated urea, however, varied with the soils used and the rate of fertilizer nitrogen applied. The results suggest that the natural products could be potential nitrification inhibitors for increasing fertilizer N use efficiency.

Biological Availability↗
PubMed2002

Effect of nickel and cadmium speciation on nitrification inhibition.

Heavy metals have been postulated to cause significant nitrification inhibition. Using biomass from a well-controlled continuously operated lab-scale nitrifying bioreactor, the effect of nickel and cadmium on ammonium and nitrite oxidation was quantified. The extent of inhibition was calculated from the kinetics of ammonium oxidation and nitrite oxidation, inferred from maximum specific oxygen uptake rates (SOUR) measured in batch respirometric assays. Nickel and cadmium inhibited ammonium oxidation but not nitrite oxidation up to total analytical concentrations of approximately 1.0 mM. Metal fractions (total and free ion) were correlated with the extent of ammonium oxidation inhibition in the presence of various metal complexing agents (EDTA, NTA, citrate, SO4(2-)). Inhibition was not a function of the total analytical metal concentration but strongly correlated with free cation concentration, [Ni2+] or [Cd2+]. This relationship could be described by either an empirical noncompetitive inhibition model for [Ni2+] or a linear model in the case of [Cd2+]. Furthermore, the free Ni2+ or Cd2+ concentrations could be modulated by the addition of a strong chelating agent (e.g., EDTA), resulting in reduced deleterious effects. However, at elevated doses, EDTA itself impaired nitrification. In sum, predictions and mandatory strategies of nitrification inhibition by heavy metals should be based on free cation concentrations and not on total metal concentrations.

Biomass↗
PubMed2003

Impact of metal sorption and internalization on nitrification inhibition.

The goal of this study was to explore the relationship between metal extracellular sorption, intracellular accumulation, and nitrification inhibition. Metal sorption on nitrifying biomass was rapid and could be described by linear partitioning with partition coefficients (Kp) of 20.3 +/- 0.1, 0.4 +/- 0.0, 0.1 +/- 0.0, and 0.2 +/- 0.0 L/g biomass chemical oxygen demand for Cu, Zn, Ni, and Cd, respectively. On the other hand, intracellular Zn, Ni, and Cd concentrations continued to increase with time beyond 12 h after metal addition, whereas intracellular Cu attained equilibrium after 4 h. Metal internalization kinetics could be described by an intraparticle diffusion model, with characteristic diffusion time constants (td) of 9.4, 64.6, 80.5, and 66.1 h for Cu, Zn, Ni, and Cd, respectively. Ultimate internalized percentages of the total cell-associated metal were 1.4 +/- 0.0, 4.3 +/- 0.5,7.6 +/- 1.0, and 2.7 +/- 0.2% for Cu, Zn, Ni, and Cd, respectively. Nitrification inhibition was not a function of the sorbed metal fraction but correlated well with intracellular Zn, Ni, or Cd fractions. An intraparticle diffusion model coupled with a saturation-type biological toxicity model fit the inhibition data for varying initial Cd concentrations and exposure periods. In contrast, no relationship between intracellular or sorbed Cu concentrations and nitrification inhibition was observed. In the presence of 1 mM Cu, less than 13.3 +/- 10.5% cells remained viable as compared to 72.8 +/- 7.5,104.8 +/- 1.7, and 84.7 +/- 7.0% (assumed 100% viable cells in metal-free control) in the presence of 1 mM Zn, Ni, and Cd, respectively. Hence, the observations that inhibition by metals such as Zn, Ni, and Cd is related to their intracellular fraction and the slow kinetics of metal internalization indicate that metal inhibition can easily be underpredicted from short-term batch assays. Furthermore, the inhibitory mechanism of Cu was very different from Zn, Ni, and Cd and may involve rapid loss of membrane integrity.

Adsorption↗
PubMed2004

Adaptation of soil biological nitrification to heavy metals.

The adaptive response of soil biological nitrification to Zn and Pb was assessed using an in situ method we have developed. The method is based on reinoculating a sterilized metal contaminated soil with the same soil that is either uncontaminated or has been incubated with metal. This approach excludes the potentially confounding effects of metal aging reactions in soils. We found added Zn concentrations which gave rise to a decrease in nitrification to 50% that of the uncontaminated soil (i.e. EC50) of 210 mg/kg for communities not previously exposed to Zn and 850 mg/kg for communities exposed to Zn for 17 months, indicating that significant adaptation of the community to Zn had occurred. Similarly, this protocol was able to demonstrate adaptation of soil biological nitrification to Pb, with EC50 values of 1960 and 3150 mg/kg for the unexposed and exposed treatments, respectively. Exposure of unadapted and adapted microbial communities to a combination of Zn and Cd showed that the presence of Cd did not lead to greater toxicity in either community. Adapted communities were not more sensitive to decreases in soil pH than unadapted communities. Prior exposure to Zn was found to confer significantly greater tolerance of the community to Pb. Prior exposure to Pb similarly conferred significantly greater tolerance of the community to Zn. Implications of the adaptive capacity of soil microbes to the development of critical threshold values for heavy metals in soil based on ecotoxicity assessments are discussed.

Adaptation, Physiological↗
PubMed2004

Nitrification by plants that also fix nitrogen.

Nitrification is a key stage in the nitrogen cycle; it enables the transformation of nitrogen into an oxidized, inorganic state. The availability of nitrates produced by this process often limits primary productivity and is an important determinant in plant community ecology and biodiversity. Chemoautotrophic prokaryotes are recognized as the main facilitators of this process, although heterotrophic nitrification by fungi may be significant under certain conditions. However, there has been neither biochemical nor ecological evidence to support nitrification by photoautotrophic plants. Here we show how certain legumes that accumulate the toxin, 3-nitropropionic acid, generate oxidized inorganic nitrogen in their shoots, which is returned to the soil in their litter. In nitrogen-fixing populations this 'new' nitrate and nitrite can be derived from the assimilation of nitrogen gas. Normally, the transformation of elemental nitrogen from the atmosphere into a fixed oxidized form (as nitrate) is represented in the nitrogen cycle as a multiphasic process involving several different organisms. We show how this can occur in a single photoautotrophic organism, representing a previously undescribed feature of this biogeochemical cycle.

Atmosphere↗
PubMed2002

Treatment of antibiotics wastewater utilizing successive hydrolysis, denitrification and nitrification.

A process consisting of anaerobic hydrolysis, denitrification, and oxidation/nitrification was proposed for simultaneous removal of carbon and nitrogen from terramycin crystallizing mother solution (TCMS), and its performance was investigated by treating diluted TCMS in a lab-scale continuous flow column system. Direct denitrification-nitrification of diluted TCMS produced a significant residual of nitrate and nitrite, which disappeared 44 days after startup of anaerobic hydrolysis column. The electron donors available to denitrification were increased by 6 times after diluted TCMS was treated in the hydrolysis column under an HRT of 2.5 h or longer. The reaction rates of organics decomposition, nitrification, and denitrification were also significantly increased due to reduction of terramycin and decomposition of complicated molecules to small molecules during anaerobic hydrolysis. The specific denitrification rate and nitrification rate increased from 0.033 d(-1) and 0.01 d(-1) to 0.045 d(-1) and 0.021 d(-1) respectively after diluted TCMS (dilution ratio: 1:4) was hydrolyzed in anaerobic hydrolysis column at a hydraulic retention time (HRT) of 4 h.

Anti-Bacterial Agents↗
PubMed2001

The effects of the flow pattern on organic oxidation and nitrification in aerated submerged biofilters.

Previous research has shown that a submerged biofilter is effective in removing organic matter and total organic nitrogen. Upflow and downflow biofilters were set up to investigate the different oxidative efficiencies on organic matter and ammonia nitrogen of different biofilter positions and to compare the biological activities of the upflow and downflow patterns. A comparison of the operational characteristics of the two types of biofilters is discussed. The main COD removal zone is 0-20 cm in the upflow biofilter, but in the downflow biofilter it occurs in the submerged zone. The nitrification process is dependent on the HRT and the residual COD. In the downflow biofilter, the efficiency of the nitrification increases with the HRT. The growth of nitrifying bacteria has a tendency to take place in higher zones in the downflow biofilter, especially for a longer HRT. Batch tests measured the biological activity of heterotrophs and nitrifying autotrophs with decomposition rates. In the upflow biofilter, the nitrification rates increased while the COD oxidative rates decreased with the height of the biofilter, so the effect of the HRT on the activity of the nitrifying autotrophs is significant. However, in the down biofilter, the activity of the nitrifying autotrophs was similar at different heights in the same HRT runs with the maximum nitrifying autotrophs activity being at an HRT of 6 hours.

Bacteria↗
PubMed2005

The potential for nitrification and nitrate uptake in the rhizosphere of wetland plants: a modelling study.

BACKGROUND AND AIMS: It has recently found that lowland rice grown hydroponically is exceptionally efficient in absorbing NO3-, raising the possibility that rice and other wetland plants growing in flooded soil may absorb significant amounts of NO3- formed by nitrification of NH4+ in the rhizosphere. This is important because (a) this NO3- is otherwise lost through denitrification in the soil bulk; and (b) plant growth and yield are generally improved when plants absorb their nitrogen as a mixture of NO3- and NH4+ compared with growth on either N source on its own. A mathematical model is developed here with which to assess the extent of NO3- absorption from the rhizosphere by wetland plants growing in flooded soil, considering the important plant and soil processes operating. METHODS: The model considers rates of O2 transport away from an individual root and simultaneous O2 consumption in microbial and non-microbial processes; transport of NH4+ towards the root and its consumption in nitrification and uptake at the root surface; and transport of NO3- formed from NH4+ towards the root and its consumption in denitrification and uptake by the root. The sensitivity of the model's predictions to its input parameters is tested over the range of conditions in which wetland plants grow. KEY RESULTS: The model calculations show that substantial quantities of NO3- can be produced in the rhizosphere of wetland plants through nitrification and taken up by the roots under field conditions. The rates of NO3- uptake can be comparable with those of NH4+. The model also shows that rates of denitrification and subsequent loss of N from the soil remain small even where NO3- production and uptake are considerable. CONCLUSIONS: Nitrate uptake by wetland plants may be far more important than thought hitherto. This has implications for managing wetland soils and water, as discussed in this paper.

Biological Transport↗
PubMed1997

Heterologous expression of heterotrophic nitrification genes.

Paracoccus denitrificans is a heterotrophic organism capable of oxidizing ammonia to nitrite during growth on an organic carbon and energy source. This pathway, termed heterotrophic nitrification, requires the concerted action of an ammonia monooxygenase (AMO) and hydroxylamine oxidase (HAO). The genes required for heterotrophic nitrification have been isolated by introducing a Pa. denitrificans genomic library into Pseudomonas putida and screening for the accumulation of nitrite. In contrast to the situation in chemolithoautotrophic ammonia oxidizers, the genes encoding AMO and HAO are present in single linked copies in the genome of Pa. denitrificans. AMO from Pa. denitrificans expressed in Ps. putida is capable of oxidizing ethene (ethylene) to epoxyethane (ethylene oxide), which is indicative of a relaxed substrate specificity. Further, when expressed in the methylotroph Methylobacterium extorquens AM1, the AMO endows on this organism the ability to grow on ethene and methane. Thus, the Pa. denitrificans AMO is capable of oxidizing methane to methanol, as is the case for the AMO from Nitrosomonas europaea. The heterotrophic nitrification genes are moderately toxic in M. extorquens, more toxic in Ps. putida, and non-toxic in Escherichia coli. Toxicity is due to the activity of the gene products in M. extorquens, and both expression and activity in Ps. putida. This is the first time that the genes encoding an active AMO have been expressed in a heterologous host.

Ammonia↗