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At least 163 records · Page 9Linked to original sources
PubMed2003

Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions.

The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 mumol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8%), 2) N-deprivation and 3) high PFD (150 mumol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k = 0.7 div day-1, 228-258 mg l-1 and 2.0 x 10(5)-2.5 x 10(5) cells ml-1 at both PFD, respectively). With increasing acetate concentration, a slight increment in growth occurred only at 85 mumol m-2 s-1. Light was the best inductive carotenogenic factor, and the highest carotenoid production (4.9 mg l-1, 25.0 pg cell-1) was obtained in cultures pre-grown in nitrate at low light. The NaCl caused an increase in carotenoid content per cell at increasing salt concentrations, but resulted in a high cell mortality and did not produce any increment in carotenoid content per volume compared to cultures grown at 150 mumol m-2 s-1. The highest carotenoid content per cell (22 pg) and astaxanthin content per dry weight (10.3 mg g-1) (1% w/w) were obtained at 85 mumol m-2 s-1 with 0.8% NaCl.

Biomass↗
PubMed1991

Isolation of Jamestown Canyon virus from boreal Aedes mosquitoes from the Sierra Nevada of California.

More than 28,000 mosquitoes in four genera were collected from high elevation (greater than or equal to 1,000 m) areas of California during 1988-89 and tested for virus by plaque assay in Vero cells. Viruses were serogrouped by enzyme immunoassay and serotyped by cross-neutralization. Six strains of Jamestown Canyon virus in the California serogroup were isolated from three species of boreal Aedes in the Aedes communis group of the subgenus Ochlerotatus. All isolates were from mosquitoes collected in Alpine County at approximately 2,300 m elevation in the Sierra Nevada. These included one virus from a pool of male Aedes cataphylla collected in immature stages, which is evidence for vertical transmission; four viruses from adult female Ae. communis (sens. lat.); and one virus from adult female Aedes hexodontus. These are the first isolations of viruses from boreal Aedes mosquitoes in California and the first reported isolations of Jamestown Canyon virus from Ae. cataphylla or Ae. hexodontus.

Aedes↗
PubMed1998

Lake Vera revisited: parity and survival rates of Anopheles punctipennis at the site of a malaria outbreak in the Sierra Nevada foothills of California.

Parity and survival rates of Anopheles punctipennis were studied at the site of a 1952 outbreak of malaria in the Sierra Nevada foothills of California with the purpose of estimating blood feeding frequency and survivorship of such populations. Anopheles punctipennis was the dominant species in landing collections conducted for 20 consecutive nights in August and September 1990. The mean parity rate of An. punctipennis was 0.82. The gonotrophic cycle was estimated to last three days based on time series analysis of the number of nulliparous and parous mosquitoes collected each day. Survivorship was estimated to be 0.79 per gonotrophic cycle and 0.92 per day. Ovarian dilatation data indicated that some females had completed eight gonotrophic cycles prior to being collected and 45% of the females completed two or more cycles. The high prevalence of multiparous individuals, high parity rate, long survivorship and a short three-day gonotrophic cycle estimate indicates that this population is long lived and has a capacity to be an efficient vector of malaria.

Animals↗
PubMed1992

Medicare program; Peer Review Organizations: revised scopes of work for the District of Columbia, Puerto Rico, the Virgin Islands, and all states except Delaware, Florida, Missouri, Montana, Nebraska, Nevada, Oklahoma, Rhode Island, South Carolina, Washington, and Wyoming--HCFA. Final notice.

This notice describes requirements for the review activities of Utilization and Quality Control Peer Review Organizations (PROs) under contract extensions of the Scope of Work for the District of Columbia, Puerto Rico, the Virgin Islands and all States except Delaware, Florida, Missouri, Montana, Nebraska, Nevada, Oklahoma, Rhode Island, South Carolina, Washington, and Wyoming. Section 1153(h)(1) of the Social Security Act requires us to publish any new policy or procedure adopted by the Secretary that affects substantially the performance of PRO contract obligations at least 30 days before the date the policy or procedure is to be used. Specifically, this notice describes the way in which PRO contract requirements are changed and explains significant changes in the PRO program (e.g., the way in which cases will be selected for review) and also describes continuing requirements. This notice also implements provisions of the Omnibus Budget Reconciliation Act of 1990.

Centers for Medicare and Medicaid Services, U.S.↗
PubMed2001

Mediterranean climate effects. II. Conifer growth phenology across a Sierra Nevada ecotone.

Growth and xylem water potential of the lower elevation conifers Pinus jeffreyi and Abies concolor and the higher elevation Pinus monticola and Abies magnifica were monitored in their montane Mediterranean habitat of the southernmost Sierra Nevada mountains of California. Measurements were made across the ecotone between the midmontane and upper montane forests and through light and heavy snowfall years.Radial stem growth, averaging ∼1.5 mm/yr, started 2 wk after snow melt, providing that maximum air temperatures had reached 21°C, and ended when predawn water potentials fell rapidly at the onset of the summer drought. Leader growth started on or after a fixed date, providing that minimum air temperatures were above -4°C for Pinus species or +2.5°C for Abies species. The cue for leader growth was inferred to be photoperiodic. Leader growth ended when either a determinate internode length of ∼1 mm was reached or predawn water potentials fell rapidly. Abies magnifica grew more rapidly than the low-elevation species, but had a shorter growth period; its annual leader growth, as a consequence, was only 35 mm/yr vs. 50 mm/yr for the low-elevation species. Needle growth was similarly determinate in the absence of early drought. This growth phenology contributes to determining species distribution across the ecotone.

Journal Article↗
PubMed1991

Influence of vegetation on carbon dioxide trap effectiveness for sampling mosquitoes in the Sierra Nevada foothills of Kern County, California.

The effect of vegetation on sampling Culex tarsalis, Cx. quinquefasciatus and Aedes nigromaculis by CO2 traps was evaluated at an intermittent stream habitat at the base of the Sierra Nevada foothills. Carbon dioxide traps were spaced along a 450 m transect perpendicular to Poso Creek to determine female attraction to traps placed in 5 different vegetation substrates: 1) open hilltop with sparse growth of grasses and saltbush, 2) open pasture with sparse growth of saltbush, 3) peripheral understory of mule fat, 4) shaded understory of mule fat, and 5) open canopy 5 m above ground in willow and cottonwood trees. Most host-seeking Cx. tarsalis and Cx. quinquefasciatus females were collected within the open canopy and peripheral understory. Host-seeking Ae. nigromaculis females were collected predominately in the open pasture and within the peripheral understory. The association between CO2 trap catch size and vegetation suggested a relationship between the host-feeding patterns and associated hunting strategies of these bird and mammal feeding species.

Aedes↗
PubMed1985

Altered energy metabolism in an irradiated population of lizards at the Nevada Test Site.

Field metabolic rates (via doubly labeled water), body compartmentalization of energy stores, and energy assimilation efficiencies were measured to assess all avenues of energy utilization in Uta stansburiana living in a low-level gamma-irradiated plot in Rock-Valley, Nevada. Comparison of energy budgets for radiation-sterilized females with those of nonirradiated control lizards revealed several substantial differences. Sterile females were heavier, mainly because they had extraordinarily large energy (fat) storage depots. Sterile females had much lower rates of energy expenditure via respiration and lower rates of energy intake by feeding. These differences are interpreted as indirect responses to radiation-induced sterility. Gastrointestinal tract function in sterile females was normal. There is little evidence of direct radiation effects on physiological functions other than reproduction.

Animals↗
PubMed1996

Salmonellosis associated with a Thanksgiving dinner--Nevada, 1995.

On November 28, 1995, the county coroner's office notified the Clark County Health District in Las Vegas, Nevada, about a death suspected to have resulted from a food-borne disease. This report summarizes the investigation of the outbreak of gastroenteritis among persons who attended a Thanksgiving dinner. The investigation documented Salmonella serotype Enteritidis (SE) infection associated with eating improperly prepared turkey and stuffing containing eggs and emphasizes the need to use a meat thermometer to ensure complete cooking of turkey and stuffing.

Animals↗
PubMed1992

Riggins v. Nevada.

Explore the source record for details and available documents.

Civil Rights↗
PubMed1995

Public cost and access to primary care for hyperglycemic emergencies, Clark County, Nevada.

Diabetes mellitus accounts for 5.8% of the total health care costs of citizens of the United States. Hospitalization expenses produce 40.5% of these costs. We sought to determine the public expenditure and major precipitators of admissions for uninsured diabetic hyperglycemic emergencies at a large public hospital. Of 247 diabetic emergency admissions over a 30 month period 49% (n = 121) of these patients had no medical insurance. The uninsured patients were younger and had relatively mild disease in comparison to the insured patients. These patients identified a primary physician in only 6% of the cases and had a higher incidence of admissions associated with lack of medications. We conclude that public funds to provide access to primary care and enhancement of employer-sponsored health insurance programs may decrease the numbers and costs of hospitalizations due to hyperglycemic emergencies in uninsured patients with diabetes mellitus.

Adolescent↗