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Malaria treatment in remote areas of Mali: use of modern and traditional medicines, patient outcome.

Use of official health services often remains low despite great efforts to improve quality of care. Are informal treatments responsible for keeping a number of patients away from standard care, and if so, why? Through a questionnaire survey with proportional cluster samples, we studied the case histories of 952 children in Bandiagara and Sikasso areas of Mali. Most children with reported uncomplicated malaria were first treated at home (87%) with modern medicines alone (40%), a mixture of modern and traditional treatments (33%), or traditional treatment alone (27%). For severe episodes (224 cases), a traditional treatment alone was used in 50% of the cases. Clinical recovery after uncomplicated malaria was above 98% with any type of treatment. For presumed severe malaria, the global mortality rate was 17%; it was not correlated with the type of treatment used (traditional or modern, at home or elsewhere). In the study areas, informal treatments divert a high proportion of patients away from official health services. Patients' experience that outcome after standard therapeutic itineraries is not better than after alternative care may help to explain low use of official health services. We need to study whether some traditional treatments available in remote villages should be considered real, recommendable first aid.

Adolescent↗

Incidence of severe Plasmodium falciparum malaria as a primary endpoint for vaccine efficacy trials in Bandiagara, Mali.

Potential endpoints for blood stage malaria vaccine efficacy trials include uncomplicated malaria disease, which is hard to differentiate from other febrile illnesses, and mortality, which requires prohibitively large sample sizes. Strictly defined severe malaria predicts malaria-associated mortality where case fatality rates are known. To assess the suitability of severe malaria as a trial endpoint, we conducted a census in 1999 and measured the incidence of severe malaria from 1999 to 2001 in Bandiagara, Mali. The annual incidence of severe malaria in children <6 years of age was 2.3% (n = 2,284) yielding an estimated sample size of 4,580 for a vaccine trial designed to detect 50% efficacy with 80% power at P = 0.05 with 5% loss to follow-up. A trial using severe malaria as an endpoint in this setting would thus require expanding the study population or the length of the trial. This approach may be useful in assessing the suitability of potential sites for malaria vaccine trials.

Child↗

Species diversity and acquisition of gastrointestinal parasites in calves aged 0-13 months in periurban livestock production in Mali.

Post-mortem inspections on 51 calves were conducted to investigate acquisition and spectrum of gastrointestinal parasites in young calves in periurban livestock production in Mali. Generalized linear models with herd as a repeated effect were used to test the influence of management, age and season of birth on parasite numbers and diversity. Parasite number and burden increased with age. In the age class 4-13 months animals carried already up to eight different gastrointestinal parasite species. Parasite spectrum found included 11 nematodes, 3 cestodes and 1 protozoan parasite. The most frequent parasite species found were Haemonchus placei (age class 0-1 month: 7%, 1-4 months: 38%, 4-13 months: 69%), Cooperia pectinata (0%, 33% and 44%) and C. punctata (0%, 33% and 38%). Calves born during the rainy season had higher parasite burden and species diversity than calves born during the dry season. Calves kept under modernised management excreted more Strongyloides papillosus eggs than calves kept under traditional management. Calves acquired most of the parasites occurring in adult bovines in West Africa within their first year of life.

Age Factors↗

The phenology of malaria mosquitoes in irrigated rice fields in Mali.

A field study was carried out in the large-scale rice irrigation scheme of the Office du Niger in Mali to investigate the relation between anopheline mosquito larval development and small-scale differences in irrigation practices, such as water level, irrigation application and irrigation frequency. The objective of the study was to find out if water management can be used as a tool for vector control to reduce the malaria transmission risk. Larvae of Anopheles gambiae s.s.,; the main malaria vector in the study area, developed mostly in the first 6 weeks after transplanting the rice. During rice development, a succession of anopheline species was observed. This was associated with a marked decrease in light intensity reaching the water surface as plant height increased. Minor differences in water management resulted in noticeable variations in larval densities and species composition. A. gambiae s.s. larvae were most abundant during the early growing stages and almost absent in a closed rice crop. Due to improper drainage after harvest, A. gambiae s.s. breeding was soon re-established in fields where small pools of water were retained. The results suggest that larval mosquito habitats in the Office du Niger can be significantly reduced by water management, simultaneous planting and harvesting and proper drainage of fallow fields.

Agriculture↗

Assessment of cure by detection of circulating antigens in serum and urine, following schistosomiasis mass treatment in two villages of the Office du Niger, Mali.

Eight weeks after mass chemotherapy with 40 mg/kg praziquantel in two villages in Office du Niger (an irrigation area in Mali, endemic for both Schistosoma haematobium and Schistosoma mansoni) the circulating anodic (CAA) and cathodic (CCA) antigen detection assays were carried out on serum and urine samples. Both prior and post treatment highest prevalence was measured with the urine-CCA assay. Cure rates determined by antigen detection were almost half that of the egg counting methods. It was shown that the reduction in intensity should be preferentially assessed by the serum-CAA assay. Compared with egg detection, a single antigen detection assay gave a much better assessment of the impact of chemotherapy.

Animals↗

Distribution and genetic diversity of Schistosoma haematobium within its bulinid intermediate hosts in Mali.

Random amplified polymorphic DNA (RAPD) markers were used to assess distribution and genetic diversity of Schistosoma haematobium populations within their bulinid intermediate hosts in Mali. Naturally infected snails (Bulinus truncatus and B. globosus) were collected at four sites in the Bamako district. S. haematobium cercariae from single snails were used to infect mice and genotypes of the resultant adult worms were characterized using RAPD markers. Diversity indices were calculated at the scale of one snail, both within and among sites. One third of the molluscs harboured multiple miracidial infections (the maximum number equal to five) with slightly overdispersed distributions in three sites and a random distribution at one site. Similarity indices revealed significantly less variation among populations compared with populations, indicative of the absence of distinct S. haematobium populations within the Bamako district. RAPD markers represent an accurate tool for determining genetic diversity and amount of gene flow among parasite populations contained within different individual snails and among intermediate host populations.

Animals↗

Epidemic of meningococcal meningitis in Bamako, Mali: epidemiological features and analysis of vaccine efficacy.

Many studies have demonstrated the efficacy of meningococcal vaccine in nonepidemic meningococcal meningitis, but few have examined its efficacy in epidemic conditions. The effects of the vaccine on the course of a meningococcal meningitis epidemic in Bamako, Mali, between January and April, 1981, were studied. The vaccine was effective in limiting further spread of the epidemic. The attack rate among those who received vaccine was lower than that in the unvaccinated (0.7/10 000 vs 4.7/10 000), and the case-fatality rate among vaccinees in whom meningitis developed was lower than that of the unvaccinated. Routine vaccination against meningococcal meningitis in Africa may be impracticable owing to the cost, the relatively short duration of effectiveness, and the irregular occurrence of epidemics. Vaccination early in the course of an epidemic, however, appears to be a useful and practicable method of limiting the spread of disease.

Bacterial Vaccines↗

Women's social networks and child survival in Mali.

This paper explores the influence of women's social networks on child survival through a comparative investigation of two ethnic groups in Mali, West Africa. Data are drawn from a study of women's social networks and health conducted during the period 1996-97. Separate samples of 500 ever-married women aged 15-49 were surveyed at two geographically distinct sites representing Bamanan and Fulbe populations respectively. Consistent with known differences in economic risk, household structure, and cultural norms, descriptive analysis reveals a greater probability of child death among the Fulbe, and a larger mean size of total, material, practical and cognitive networks among the Bamanan. Cox regression models are used to examine the association between social network size, function and composition and the odds of child death (1-5 years). Among the various biological, household and community-level variables tested in the basic model, spacing exerts an expected negative effect on the odds of child death in both groups, while household SES predicts child survival only among Fulbe children. When variables representing the educational and psychosocial attributes of the mother are included, no effects are detected in either group. Controlling for these factors, the size of total, practical, cognitive and emotional networks are found to significantly increase the odds of child survival among the Fulbe only. Compositional variables, such as the extent to which natal kin, non-kin or husbands figure in a woman's network, nor the degree to which networks are located within household yield any significant results for the Fulbe. Among Bamanan women, however, the higher the proportion of network members living in the household, the lower the odds of child death. The paper concludes by discussing the methodological, conceptual and practical implications of these findings.

Adult↗

Public and private sector responses to essential drugs policies: a multilevel analysis of drug prescription and selling practices in Mali.

Many African countries have introduced cost recovery mechanisms based on the sale of drugs and measures aimed at improving drug supply. This study compares prescribing and selling practices in Mali, in 3 cities where the public sector contributes differentially to the supply of drugs on the market. Multilevel models are used to analyse the content and cost of 700 medication transactions observed in 14 private and public legal points of sale. Results show that the objective of improving access to drugs seems to have been achieved in the sites studied. Costs of prescriptions were lower where public health services had been revitalized. Affordable generic drugs were accessible and widely used, even in the private sector. However, measures intended to rationalize the prescription and delivery of drugs did not always have the desired effect. While agents in the public sector tended to prescribe fewer antibiotics, injectables, or brand-name drugs, the data confirm the virtual absence of advice concerning the use or the side effects of the drugs in both public and private sectors. In addition, data supported the notion that the public and private sectors are closely intertwined. Notably, availability of drugs in the public sector contributed to diminishing the prices charged in the private sector. Similarly, the use that agents in the public sector made of the opportunities afforded by the presence of the private pharmaceutical sector provided another illustration of interrelatedness. Finally, the data showed that the presence of a private sector, which has not been affected by measures aimed at rationalizing prescription and sales practices, limits the effects of measures implemented in the public sector. More assertive policies, based on strategies encompassing actors in the private sector, are needed to increase the safety and effectiveness of prescription and sales practices.

Community Health Centers↗

Endogamy among the Dogon of Boni, Mali.

This paper examines factors influencing endogamy in a Dogon population in Mali. Situated in Boni district, this population of about 5000 individuals is distributed over fifteen villages located on four independent massifs. This population is strongly endogamous (only 4% of all marriages are contracted with neighbouring ethnic groups), and each massif shows high endogamy. The roles of lineage, residence in the same village, and geographical distance in mating choice are examined. These different factors are successively analysed using log-linear statistical models and the results offer a more precise interpretation of endogamy in this population.

Consanguinity↗

Women's social power, child nutrition and poverty in Mali.

While the macro-level association between poverty and child malnutrition is well-established, the concept of 'poverty' and its operationalization in terms of measures of socioeconomic status shed little or no light on the mechanisms through which malnutrition is created and/or prevented. This paper investigates a woman's social power, one such mechanism that may mediate the impact of poverty on childhood nutrition. This micro-level factor is examined using survey data on 402 children 5 years of age and younger and their 261 Fulbe mothers in rural Mali. A conceptual model of social power is developed and used to test the hypothesis that a mother's social power can predict her child's nutritional status.

Adolescent↗

Schistosomiasis in the Senegal River Basin: before and after the construction of the dams at Diama, Senegal and Manantali, Mali and future prospects.

Ecological changes in the Senegal River Basin (SRB) resulting from the construction of a barrage at Diama, Senegal on the Senegal River to prevent the intrusion of sea water into the river, and a dam at Manantali, Mali on the Bafing River to control the flow of water and to generate electricity, have been responsible for changes in the epidemiology of human schistosomiasis. The introduction of Schistosoma mansoni into the Lower and Middle Valleys of the SRB and subsequent spread of the parasite in the human population is recorded with regard to prevalence and intensity. New foci of S. haematobium are described. The reduction in salinity and change from an acidic to an alkaline environment in the water are beneficial to both the fecundity and growth of freshwater snails and transmission of the parasite. The creation of new irrigation canals and expansion of the rice fields have provided new habitats for intermediate hosts to colonize. The evidence for praziquantel resistance/tolerance by populations of S. mansoni and the possibilities of the development, production and testing of a vaccine against human schistosomiasis are discussed. Future studies will monitor the spread of human urinary and mesenteric schistosomiasis in the SRB, will evaluate further the presence of praziquantel resistance/tolerance in S. mansoni, will examine the heavily infected human population for pathological symptoms and determine the most appropriate methods to control this severe outbreak of human schistosomiasis.

Animals↗

Epidemic cholera in Mali: high mortality and multiple routes of transmission in a famine area.

During the 1984 cholera epidemic in Mali, 1793 cases and 406 deaths were reported, a death-to-case ratio of 23%. In four affected villages, the mean clinical attack rate was 1.5 and 29% of affected persons died. In 66% of cases the illness began more than 48 h after the village outbreak began, when supplies from outside the village were potentially available. Deaths occurred because patients failed to seek care or received only limited rehydration therapy when they did. Case-control studies identified two routes of transmission: drinking water from one well in a village outside the drought area, and eating left-over millet gruel in a drought-affected village. Drought-related scarcity of curdled milk may permit millet gruel to be a vehicle for cholera. Cholera mortality in the Sahel could be greatly reduced by rapid intervention in affected villages, wide distribution of effective rehydration materials, and educating the population to seek treatment quickly.

Adolescent↗

Food variety, socioeconomic status and nutritional status in urban and rural areas in Koutiala (Mali).

OBJECTIVE: The purpose of this study was to analyse the associations between the food variety score (FVS), dietary diversity score (DDS) and nutritional status of children, and to assess the associations between FVS, DDS and socioeconomic status (SES) on a household level. The study also assessed urban and rural differences in FVS and DDS. DESIGN: Cross-sectional studies in 1994/95, including a simplified food frequency questionnaire on food items used in the household the previous day. A socioeconomic score was generated, based on possessions in the households. Weight and height were measured for all children aged 6-59 months in the households, and anthropometric indices were generated. SUBJECTS AND SETTING: Three hundred and twenty-nine urban and 488 rural households with 526 urban and 1789 rural children aged 6-59 months in Koutiala County, Sikasso Region, Mali. RESULTS: Children from urban households with a low FVS or DDS had a doubled risk (OR>2) for being stunted and underweight. Those relations were not found in the rural area. There was an association between SES and both FVS and DDS on the household level in both areas. The FVS and DDS in urban households with the lowest SES were higher than the FVS and DDS among the rural households with the highest SES. CONCLUSIONS: Food variety and dietary diversity seem to be associated with nutritional status (weight/age and height/age) of children in heterogeneous communities, as our data from urban areas showed. In rural areas, however, this association could not be shown. Socioeconomic factors seem to be important determinants for FVS and DDS both in urban and rural areas. FVS and DDS are useful variables in assessing the nutritional situation of households, particular in urban areas.

Body Height↗

A complement fixing polysaccharide from Biophytum petersianum Klotzsch, a medicinal plant from Mali, West Africa.

Biophytum petersianum Klotzsch (syn. Biophytum sensitivum (L.) DC) is a medicinal plant having a traditional use, among others, as a wound healing remedy in Mali and other countries. As a water extract of the aerial parts of the plant is a frequently used preparation, we decided to look for a bioactive polysaccharide in this extract. One of the obtained polysaccharide fractions, BP100 III, isolated from a 100 degrees C water extract from the aerial parts of B. petersianum and having a monosaccharide composition typical for pectic substances, was shown to exhibit potent dose-dependent complement fixating activity. The BP100 III fraction was subjected to degradation by endo-alpha-d-(1-->4)-polygalacturonase, and three fractions were obtained by gel filtration. The highest molecular weight fraction, BP100 III.1, had a more potent activity in the complement test system than the native polymer, while the two lower molecular weight fractions were less active than the native polymer. The major part of BP100 III.1 consists of galacturonic acid and rhamnose, with branches being present on both the rhamnose and galacturonic acid residues. Arabinogalactan type II is also present in the polymer, indicating that BP100 III.1 has a structure typical of the hairy region of pectins. The major part of the two other fractions is a galacturonan, containing a strikingly high number of branch points, some to which xylose is attached. These results indicate that the pectic substance in B. petersianum contains both rhamnogalacturonan and xylogalacturonan regions.

Animals↗

Effect of suckling regime on calf growth, milk production and offtake of zebu cattle in Mali.

In order to assess the effect of suckling regime on calf growth and milk yield and offtake of zebu cattle in Mali, 59 zebu cow-calf pairs were allocated to suckling regimes: RI, cows were milked completely and the milk was bottle-fed ad libitum to their calves; RII, calves were suckled ad libitum by their dams prior to milking; RIII, cows were milked partially then allowed to suckle their calves; RIV, ad libitum suckling of calves prior to milking during the first three months of lactation and partial milking prior to suckling thereafter. The highest lactation milk yield (1150.6 kg) and offtake (647.6 kg), but the poorest calf growth (261 g/d to weaning) were observed in RIII. RIV significantly improved calf growth, 310 g/d, while allowing a milk offtake of 294.4 kg.

Animal Feed↗

Urbanisation and vitamin A deficiency in children: comparison between a traditional district and a new settlement in Mali.

OBJECTIVES: The purpose of this study was to assess the vitamin A status of pre-school urban children and to compare the situation between a traditional district and a new settlement after two decades of intense urbanisation. SUBJECTS AND METHODS: A cross-sectional stratified survey was carried out in Bamako, Mali, on a representative sample of children (aged 4-6 years). 532 children were involved from a traditional district and 453 from a new settlement (NS). The vitamin A status was assessed by: clinical indicators (ophthalmic examination), subclinical indicators (questionnaire looking for night blindness, impression cytology with transfer test (ICT), modified relative dose response test (MRDR), and a diet inquiry about vitamin A intakes. Acute malnutrition was assessed by a weight/height measure. RESULTS: In both districts, all the clinical indicators were below the WHO criteria that define a public health problem. Both the ICT test, respectively 19 and 21% of abnormal tests, and the MRDR, 67.3 and 73.1% of tests > or = 0.06, indicated a subclinical vitamin A deficiency as defined by WHO thresholds. During the preceding week only four children in NS had consumed vitamin A-rich food less than seven times. No significant difference between the two districts was found either for clinical or subclinical indicators (p > 0.5). CONCLUSION: Despite a rapid urbanisation, the vitamin A status of the children seemed to be rather homogeneous among the different districts. The population remained vulnerable with peripheral depletion and low hepatic stores of vitamin A. The urban children should be carefully monitored regarding vitamin A status.

Anthropometry↗

Food variety--a good indicator of nutritional adequacy of the diet? A case study from an urban area in Mali, West Africa.

OBJECTIVE: This study assesses whether a simple count of food items and food groups can predict the nutritional adequacy of the diet in an economically poor country. DESIGN: A three-day weighed record of children. SETTING: Koutiala town, in Southeastern Mali. SUBJECTS: Seventy-seven children, 13-58 months of age. One child was excluded owing to an extraordinarily low food variety. INTERVENTION: The study was conducted in April August 1995. Data from this study were used to create two different indices: Food Variety Score (FVS), a simple count of food items, and Dietary Diversity Score (DDS), a count of food groups. Mean Adequacy Ratio (MAR) was calculated as an indicator for nutrient adequacy, and used to validate FVS and DDS. RESULTS: Mean (s.d.) FVS was 20.5 (3.8) and mean (s.d.) DDS was 5.8 (1.1). A positive correlation was found both between FVS and MAR (Pearson 0.33, P < 0.001) and DDS and MAR (Pearson 0.39, P < 0.001). With cut-off points for FVS at 23 and for DDS at 6, the indices have high ability to identify those with a nutritionally inadequate diet. MAR increased with increasing FVS and DDS. FVS needs to be at least 15 or DDS at least 5 to give a satisfactory MAR. CONCLUSION: Although a simple count of food items or food groups cannot give a full picture of the adequacy of the nutrient intake, the results from this study show that the food scores can give a fairly good assessment of the nutritional adequacy of the diet, particularly if combined. Such indicators are important for identification of vulnerable groups in areas where people normally eat from a shared bowl, which makes detailed dietary intake studies difficult, time consuming and expensive.

Child Nutritional Physiological Phenomena↗