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At least 163 records · Page 9Linked to original sources
PubMed1975

[Laboratory procedures in adenoviruses. II. Sensitivity of various cell cultures as determined by endpoint titration (author's transl)].

The sensitivity of human embryonic kidney (HEK), human amnion (HAm) and HeLa cells for human adenoviruses was investigated by means of endpoint titrations of 13 prototype strains and numerous original specimens from patients. Prototypes and original specimens showed the same behavior. Adenoviruses of subgrouppp III produced nearly identical titers in all 3 cell cultures, while for subgroup I HAm cells showed a 10- to 100-fold lower titer. On the other hand, HAm cells are nearly as sensitive as HEK cells for type 8, whereas HeLa cells are less sensitive. For other viruses of subgroup II and for subgrou IV, HAm (for type 12 also HeLa) cells are less sensitive than HEK cells. Out of three HeLa cell strains tested the Bristol strain was inferior to the other two in its sensitivity. Concerning the speed of the CPE development, HEK cells are superior for all types, the difference being greatest for subgroup I towards HAm and HeLa and for type 8 towards HeLa cells. The length of incubation necessary for the appearance of CPE even with minimal amounts of virus is 40 days for type 8 in all kinds of cell cultures, whereas for adenoviruses of sub-groups I and III, which are predominantly isolated in the routine laboratory, 25 days for HEK and 30 days for HAm and HeLa cells may suffice in most cases. When endpoint titrations are compared with titers obtained by immunofluorescence, it appears that this technique may be applied as a screening method; thus long incubation periods of negative specimens can be saved. The amounts of infectious virus present in original specimens are reported; the highest quantity was found in specimens containing subgroup I viruses.

Adenoviridae↗
PubMed1985

Symposium on semiprecision attachments in removable partial dentures. Laboratory procedures.

The fabrication of semiprecision rest systems will differ depending on whether a prefabricated rest system or milled-in rest system is indicated. In a prefabricated rest seat, a die-related model must be used and the rest seats must be waxed into the wax pattern. A means of fabricating both locking and nonlocking rests has been described. The instruments and tools necessary for the milled-in rest seat have been illustrated. The need for a stress-releasing attachment for distal-extension removable partial dentures is satisfied by the Thompson dowel rest. In fabricating the Thompson dowel rest prosthesis, both vertical parallelism and horizontal parallelism are essential for rotation. The use of a parallelometer and the parallel rule insures precise positioning of the Thompson dowel rest, which in turn, will enable the distal extension partial to rotate.

Dental Abutments↗
PubMed1989

[Diagnosis of ovarian insufficiency: clinical and analytic laboratory procedures].

The diagnostic procedure in ovarian insufficiency includes a subtle anamnesis of the patients history, general clinical examination, investigation of the cylically dependent factors (e.g. cervical mucus), ovarian sonography and analysis of the relevant hormonal parameters. For the exact interpretation of hormone values it is essential to relate on biologically proven normal values which can best be obtained in spontaneous conception cycles.

Amenorrhea↗
PubMed1984

Modified clinical and laboratory procedures for fabrication of nasal prostheses.

A modified procedure that aids in the fabrication of a nasal prosthesis has been described. This method offers several distinct advantages over traditional techniques. These are (1) use of the silicone putty impression material reduces sculpturing time, (2) the tinted wax allows improved patient acceptance of the wax model at the try-on, and (3) use of conditioning material at the try-on improves marginal adaptation.

Humans↗
PubMed1989

Laboratory procedures for the one-clasp removable partial denture.

The lateral rotational path of insertion for removable partial dentures in the Kennedy class II category is generally limited to situations where the dentulous side has been restored with fixed restorations that provide a lingual undercut. The rigid lingual plate is first inserted horizontally into the undercut, followed by a lateral rotation to seat the edentulous side. No alterations of properly designed fixed restorations are necessary. This article discusses the technical procedures in detail.

Dental Abutments↗