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PubMed1992

Population growth with variable family size.

"The Sharpe-Lotka continuous time deterministic model of population growth is developed to take account of some possible forms of mother-daughter fertility association.... Model specific results relating the intergenerational fertility effect to the long term population growth rate and magnitude are established. The quantitative implications of the theory are illustrated by a consideration of a general bilinear form of A and in this context numerical results illustrating the finite time growth and also the long term distribution of fertility levels in the stable female population are obtained. In particular, it is shown that different fertility specific subpopulations can coexist indefinitely."

Demography↗
PubMed1986

Human capital and the rise and fall of families.

"This paper develops a model of the transmission of earnings, assets, and consumption from parents to descendants. The model assumes utility-maximizing parents who are concerned about the welfare of their children. The degree of intergenerational mobility is determined by the interaction of this utility-maximizing behavior with investment and consumption opportunities in different generations and with different kinds of luck. We examine a number of empirical studies for different countries. Regression to the mean in earnings in rich countries appears to be rapid. Almost all the earnings advantages or disadvantages of ancestors are wiped out in three generations." A comment by Robert J. Willis is included (pp. 40-7).

Behavior↗
PubMed1986

Unobservable family and individual contributions to the distributions of income and wealth.

"This paper uses combinations of full brothers, half brothers, and fathers and sons to measure the effect of common family background on a household's income and wealth. While the data are drawn from a nineteenth-century [U.S.] population, the intraclass correlation for income ranges from .13 to .18, which is similar to that found in modern samples. Intraclass correlations for wealth are significantly higher (.18-.35) than are those for income. Intraclass correlations of half brothers compared to those for full brothers suggest that fathers play a dominant role in the transmission of the common family effect. When unobserved background is decomposed into individual and family effects, the individual effect dominates the family effect for income, while the family effect dominates the individual effect for wealth." A comment by Sherwin Rosen is included (pp. 80-2).

Americas↗
PubMed1993

Country estimates of social discount rates based on changes in life expectancies.

"When time is the numeraire in a social evaluation of public projects, life expectancies are essentially the unit of account. Since different generations have different life expectancies, pursuing intergenerational equity requires discounting the extra time that future generations are likely to obtain. The result is a Social Discount Rate set by the growth rate in life expectancies. This is called the Life Expectancy Discount Rate (LEDR). After providing estimates of the LEDR for 120 countries, the paper discusses some of the implications of using this rate." (SUMMARY IN FRE AND GER)

Demography↗
PubMed1991

The greying of the baby boom in the United States: framing the policy debate.

"Issues surrounding the retirement of the large post-World War II 'baby boom' generation in the United States are often framed either in terms of impending intergenerational crisis or as an event to which the United States can gradually adjust. This paper examines these two perspectives as they compete to define U.S. social security policy. It suggests that the intergenerational crisis perspective functions primarily as an ideological argument and strategy for de-structuring social welfare policy. In contrast, the gradual adjustment perspective provides a stronger basis for planning social security and other policy responses to the ageing of the baby boom. However, it is not informed by a clear social vision and has not, as yet, responded sufficiently to the potential divisiveness which may accompany changing demography. Also, to date, relatively little attention has been paid to the diverse social and economic circumstances of baby boomers and the implications of this diversity for retirement planning."

Americas↗
PubMed1994

Sequence analysis of PER-1 extended-spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta-lactamases.

We have determined the nucleotide sequence (EMBL accession number, Z 21957) of the cloned chromosomal PER-1 extended-spectrum beta-lactamase gene from a Pseudomonas aeruginosa RNL-1 clinical isolate, blaPER-1 corresponds to a 924-bp open reading frame which encodes a polypeptide of 308 amino acids. This open reading frame is preceded by a -10 and a -35 region consistent with a putative P. aeruginosa promoter. Primer extension analysis of the PER-1 mRNA start revealed that this promoter was active in P. aeruginosa but not in Escherichia coli, in which PER-1 expression was driven by vector promoter sequences. N-terminal sequencing identified the PER-1 26-amino-acid leader peptide and enabled us to calculate the molecular mass (30.8 kDa) of the PER-1 mature form. Analysis of the percent GC content of blaPER-1 and of its 5' upstream sequences, as well as the codon usage for blaPER-1, indicated that blaPER-1 may have been inserted into P. aeruginosa genomic DNA from a nonpseudomonad bacterium. The PER-1 gene showed very low homology with other beta-lactamase genes at the DNA level. By using computer methods, assessment of the extent of identity between PER-1 and 10 beta-lactamase amino acid sequences indicated that PER-1 is a class A beta-lactamase. PER-1 shares around 27% amino acid identity with the sequenced extended-spectrum beta-lactamases of the TEM-SHV series and MEN-1 from Enterobacteriaceae species. The use of parsimony methods showed that PER-1 is not more closely related to gram-negative than to gram-positive bacterial class A beta-lactamases. Surprisingly, among class A beta-lactamases, PER-1 was most closely related to the recently reported CFXA from Bacteroides vulgatus, with which it shared 40% amino acid identity. This work indicates that non-Enterobacteriaceae species such as P. aeruginosa may possess class A extended-spectrum beta-lactamase genes possibly resulting from intergeneric DNA transfer.

Amino Acid Sequence↗
PubMed1987

Intergeneric protoplast fusion between Fusobacterium varium and Enterococcus faecium for enhancing dehydrodivanillin degradation.

Intergeneric protoplast fusion between Fusobacterium varium (Pcs Glu+) and Enterococcus faecium (Pcr Glu-) was performed under strictly anaerobic conditions to improve dehydrodivanillin (DDV) degradation. The fusion frequency obtained from the selective medium (Pc+ Glu-) was about 0.9 X 10(-5) to 1.3 X 10(-5). The seven fusants isolated were all gram-negative anaerobes with rod shapes like that of F. varium and with main phenotypical properties of cocci like those of E. faecium such as esculin and starch hydrolysis, milk clotting, and lactate production. Five fusants showed enhanced DDV degradation activities that were 2 to 4 times higher than those of parental strains. Genetic relatedness between a fusant (FE7) and the parents was estimated by DNA-DNA Southern blot hybridization with 32P-labeled chromosomal DNA fragments of F. varium and E. faecium as respective probes. The fusant FE7 presented a very high cross-hybridization with both probes, indicating a high DNA homology between the fusant and both parental strains. Almost all the fusants obtained here have stably kept the properties described above for about 2 years. These results suggest that intergeneric gene transfer takes place through protoplast fusion and that the fusants that were obtained are stable recombinants.

Anaerobiosis↗
PubMed2003

Intergeneric conjugation in Streptomyces peucetius and Streptomyces sp. strain C5: chromosomal integration and expression of recombinant plasmids carrying the chiC gene.

Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. strain C5 were transformed using E. coli ET12567(pUZ8002) as a conjugal donor. When this donor species, carrying pSET152, was mated with Streptomyces strains, the resident plasmid was mobilized to the recipient and the transferred DNA was also integrated into the recipient chromosome. Analysis of the exconjugants showed stable integration of the plasmid at a single chromosomal site (attB) of the Streptomyces genome. The DNA sequence of the chromosomal integration site was determined and shown to be conserved. However, the core sequence, where the crossover presumably occurred in C5 and S. peucetius, is TTC. These results also showed that the phiC31 integrative recombination is active and the phage attP site is functional in S. peucetius as well as in C5. The efficiency and specificity of phiC31-mediated site-specific integration of the plasmid in the presence of a 3.7-kb homologous DNA sequence indicates that integrative recombination is preferred under these conditions. The integration of plasmid DNA did not affect antibiotic biosynthesis or biosynthesis of essential amino acids. Integration of a single copy of a mutant chiC into the wild-type S. peucetius chromosome led to the production of 30-fold more chitinase.

Attachment Sites, Microbiological↗
PubMed1976

Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli.

Study of many of the interesting properties of Klebsiella aerogenes is limited by the lack of a well-characterized genetic system for this organism. Our investigations of the evolution of the enzyme ribitol dehydrogenase (EC 1.1.1.56) in K. aerogenes would be greatly facilitated by the availability of such a system, and we here report two approaches to developing one. We have isolated mutants sensitive to the coliphage P1, which will efficiently tranduce genetic markers between such sensitive strains and which will thus make detailed mapping studies possible. Derivatives of K. aerogenes lysogenic for P1 can be readily isolated by using the specialized transducing particle P1CMclr100. Bacteria lysogenic for this phage are chloramphenicol resistant and temperature sensitive. Phage particles produced by temperature induction of such lysogens can be used to transfer K. aerogenes genes to the natural host of P1 phage. Escherichia coli. We have used this method to prepare derivatives of E. coli K-12 carrying the K. aerogenes genes conferring the ability to metabolize the pentitols ribitol and D-arabitol. We have shown that these E. coli-K. aerogenes hybrids synthesize a ribitol dehydrogenase with the properties of the K. aerogenes enzyme and have mapped the position of the transferred gene on the E. coli chromosome. The ramifications of this methodology are discussed.

Alcohol Oxidoreductases↗
PubMed1987

Pediococci and biotechnology.

Nomenclature changes of pediococci postdate the publication of Bergey's Manual. Pediococci possess both a "group" and a "type" antigen. They are gram positive, asporogenous, nonmotile, generally catalase negative, but may possess catalase-like activity. The pediococci may have either a cytochrome or a flavoprotein enzyme system. Anaerobically they are homofermentative using the PEP:PTS and the EMP pathway. Catalase positive strains utilize glucose aerobically and anaerobically while lactose and glycerol are only used aerobically. Some pentoses are fermented to lactate and acetate. Absolute requirement for folinic acid and nearly all amino acids is observed. Pediococci grow luxuriously in All Purpose Tween (APT) broth and are isolated on Rogosa SL agar. Detection can be done by electrical impedance and fluorescent antibody techniques. The Arrhenius concept was utilized in selecting metabolically efficient strains. Antibiotics, antioxidants, some chloride salts and some spices are detrimental to the pediococci. On the other hand, some chloride salts, manganese, and some spices are stimulant. Dialysis-fermentation and immobilization of pediococcal cells were recorded. Some strains decarboxylate histidine to histamine. The resting cell metabolism and the production of bacteriocin have been utilized in antibiosis. An intra and intergeneric genetic transfer system of plasmids from pediococci was by a conjugation-like mechanism. Formation of bacteriocin and fermentation of carbohydrates were linked to plasmids. Lytic bacteriophages to pediococci have not yet been identified. Industrial cultures are mainly frozen concentrates. Linear equations were developed to model the fermentative activity of pediococci and the effects of environmental factors.

Antibiosis↗
PubMed1987

[The social parameters of family reproduction in Saguenay (1842-1911)].

"The family files in the population network files of the Saguenay [Canada] are used here to isolate the social parameters of family reproduction in the region between 1842 and 1911, which corresponds to the period of population settlement. Particular attention is paid to a) the kinship tie as a support of rural society, as studied through several forms of coresidence, b) the inequalities accompanying the transmission of land and the establishment of children, and c) literacy as an indicator of social differentiation. It was observed that, despite disparities created between elder and younger sons and between boys and girls, family reproduction pursued an egalitarian course. This orientation was to be compromised, however, by the tensions and constraints accompanying the saturation of arable lands around 1920 to 1930." (SUMMARY IN ENG AND SPA)

Agriculture↗
PubMed1990

Population aging and social security: a politico-economic model of state pension financing.

"The present paper attempts to provide a positive, politico-economic explanation of actual social security policies [in developed countries]. A theoretical framework is devised which integrates individual utility maximization and governmental maximization of expected political support. Individual support depends on how net economic benefit from a pay-as-you-go financed state pension scheme is translated into a probability of voting for the government. The relation between net economic positions, public policy parameters, and voting probabilities is made explicit by referring to the logit model of qualitative choice. The analysis is set in an overlapping generations framework. Optimal state pension policies are characterized, relating such diverse factors as population aging, political power distribution, social solidarity, and income taxation." (SUMMARY IN FRE AND GER)

Demography↗
PubMed1993

Aging, intergenerational distribution and public pension systems.

"This paper develops an intertemporal simulation model capable of addressing the macroeconomic and distributional effects of demographic shocks in a small open economy. Two sources of population aging are examined, viz. lower birth rates and prolonged expected lifetimes at retirement age. Due to strong expectational effects, both shocks are found to change average consumption in a downward direction, in the short run as well as in the long run. This effect is matched by a strong net acquisition of foreign assets. Furthermore, it turns out that the intergenerational distribution of the burden of adjusting to an aging population is strongly dependent on whether the benefit rate, the contribution rate, or the relative non-capital income of pensioners and workers is held fixed."

Demography↗
PubMed1993

Public pensions and declining fertility in a small open economy: an intertemporal equilibrium approach.

"The aging of the population in several industrial countries has raised concern about the financing of pay-as-you-go public pension schemes. This paper employs a numerical applied general equilibrium model of the Netherlands to explore how a unilateral temporary decline in fertility affects a small open economy. It focuses on intergenerational distributional effects as well as on macroeconomic consequences for employment, saving, investment, and external trade and capital flows. Furthermore, it discusses several policy options involving the public pension schemes to cope with the intergenerational distributional and macroeconomic effects of the decline in fertility."

Birth Rate↗
PubMed1986

A measurement scale for the parental underinvestment concept.

"The concept of parental underinvestment was used by Scrimshaw (1978) to explain infant and childhood mortality where a biological explanation was lacking. This report describes an underinvestment scale developed and tested in the Philippines. No previous scale had been developed to test the underinvestment concept. Reliability and validity of the scale are reported along with recommendations for improvement of the scale and an expansion of the parental underinvestment concept to explain outcomes other than mortality."

Adolescent↗