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[Fecal and serum anti-amebic antibodies in acute intestinal amebiasis].

Antiamebic antibody titers, were investigated in the feces and sera of 30 adults patients with intestinal amoebiasis, during the first two weeks of disease. Also, 29 control samples of healthy adult subjects were studied. The objective of this study was to determine if the local or systemic IgG, IgM or IgA levels were modified during the early stages of acute intestinal amoebiasis, for this purpose ELISA technique was use. Anti E. histolytica antibody titers in fecal samples of patients were not statistically different from titers detected in control samples, however in both groups IgA and IgM titers were higher than IgG levels. In serum samples IgG antibody titers were 4 fold higher in patients than in control group. With a P less than 0.006. In the case of IgM and IgA differences observed between the two groups studied were not statistically significant. Our results suggest that titration of coproantibodies against E. histolytica have not predictive value for intestinal amoebiasis in areas, were amoebiasis is a endemic disease. On the other hand we observed a discrete increase in IgG levels in the group of patients.

Acute Disease↗

Diagnosis of intestinal amebiasis using salivary IgA antibody detection.

This investigation sought to determine whether detection of salivary IgA antibodies to Entamoeba histolytica could identify intestinal amebic infections among 223 school children. Four groups of children were identified through coproparasitoscopic examination: E. histolytica as other parasites only (20%); and parasite-free (25%). The diagnostic accuracy of salivary IgA antibodies to an E. histolytica membrane extract was 91.5% (sensitivity, 85%; specificity, 98%), maintaining high predictive value at different prevalences. Also, a positive correlation (r = .753, P less than .001) was observed between fecal E. histolytica membrane antigen levels and salivary IgA antibody activity. Measurement of IgA antibodies in saliva may be useful in diagnosing intestinal infections with E. histolytica within a wide range of prevalences. Moreover, sampling of saliva may be a useful non invasive test for immunoepidemiologic surveys.

Adolescent↗

[The clinical picture of the initial period of intestinal amebiasis].

Clinical symptoms of the initial stage of the disease (before the onset of pathognomonic signs of amoebiasis--the formation of ulcers in the colon) have been analysed in 287 patients with intestinal amoebiasis (IA). The following variants of the clinical pattern have been identified: slow-progressing IA (59.2% of the patients examined) and fast-progressing IA (40.8% of the patients examined). In 5 patients with body eight deficit and clinical manifestations of vitamin C and B deficiency the disease assumed a fulminant course. The patients' condition was considered severe or very severe already on the first days of the infection. The results of endoscopic studies (rectoromanoscopy, fiber colonoscopy) demonstrate that the formation of amoeba-induced damages in the colon mucosa takes place by the 6th-14th day of the disease onset.

Abdominal Pain↗