Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Guinea”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources

Patterns of child growth in Papua New Guinea and their relation to environmental, dietary and socioeconomic factors--further analyses of the 1982-1983 Papua New Guinea National Nutrition Survey.

Child growth and nutrition in rural Papua New Guinea vary widely among different environments. The 1982-1983 National Nutrition Survey (NNS) was re-analyzed in order to relate patterns of growth to a wide range of dietary, socioeconomic, agricultural and demographic variables. Anthropometric indices of growth were calculated based on an internal Papua New Guinean growth standard constructed from the children included in the NNS. Children were subsequently classified as stunted, wasted or underweight if they were more than 1 SD below the national mean. Regression analyses on 15,975 children show that variation in growth among environments can largely be accounted for by differences in diet, although significant differences in relation to altitude, relief and rainfall patterns persist. Other important predictors of child growth and nutrition included socioeconomic status, maternal education, marital status of the mother and father's occupation. Variance components analysis revealed that most of the geographical variation in child growth was accounted for by the environmental, dietary, socioeconomic, agricultural and demographic variables included in the regression analyses. Most of the factors which were found to be associated with child growth in this study are related in one form or another to differences in local subsistence agriculture, which may therefore be the main determinant of child growth and nutrition patterns in Papua New Guinea.

Anthropometry↗

[The effect of urine from castrated male or female guinea pigs and rats on the estrous cycle of guinea pigs and rats, respectively].

A 24-hour reduced cycle duration was observed in 5-day cyclic female rats exposed to the odor of urine from male or female castrated rats. A decrease in the duration of the period of vaginal closure, ranging from 2 to 5 days, was observed in female guinea pigs exposed to the odor of urine from male or female castrated guinea pigs. The pheromonal activity of urine in both species was concluded to be no dependent upon the gonadal function.

Animals↗

[The experimental study of guinea pig cytomegalovirus infection in the kidney of the pup of guinea pig].

OBJECTIVE: To study the relationship of guinea pig cytomegalovirus (GPCMV) infection with the outcome of pregnancy by the kidney of guinea pig (GP). METHODS: Twenty first-trimester gestation GPs were randomly selected, intraperitoneally inoculated with GPCMV. Then female GPs and the pups were killed within 24 h after delivery. By in situ hybridization (ISH) with three phases GPCMV late-mRNA probes labeled by digoxin, the virus load and its distribution were screened inside the pup's kidney. RESULTS: Twenty GPs totally conceived 63 pups. Among them, 42 had normal outcome and lived longer than 24 h; 21 had abnormal outcome such as abortion, fetal death, et al. By in situ hybridization, the infection rate of normal pups was 7.1% (3/42) and the average optical density (A) was 0.105 +/- 0.052. The infection rate of abnormal pups was 28. 6% (6/21) and the A was 0.158 +/- 0.047. The difference of the A was significant (t = 2.57, P < 0.05). The positive signal of ISH was mainly distributed in the epithelium of renal tubule and collecting duct. CONCLUSION: It is concluded that the late-mRNA mainly expressed in the epithelium of renal tubule and collecting duct and the expression level was related with the abnormal pregnancy outcome.

Animals↗

[Comparative histological studies of mouth mucosa, gingiva and desmodont in normal guinea pigs and guinea pigs fed a vitamin C deficient diet].

The changes occurring in the oral mucosa, gingivae and periodontium of guinea pigs fed a vitamin C-deficient diet were determined by means of histological comparison with animals on a normal diet. The following results were obtained from the scorbutic guinea pigs: --detachment of the horny layer from the underlying epithelium, --bullous cells in the spinose and the granular layer, --formation of periodontal pockets, --reduction in number and disorientation of collagenous fibres of the periodontium associated with loosening of the molars.

Animals↗

Effects of neurotensin in feline pial arteries, guinea-pig ileum and portal vein of rat and guinea-pig.

The effect of neurotensin (NT) was investigated in several vascular and intestinal smooth muscle preparations. No effect of NT (6 X 10(-12)-6 X 10(-5) M) was detected in feline pial arteries in situ employing perivascular microapplication and TV-image splitting for the measurement of vascular diameter. In vitro, the effect of NT was derived from recordings of isometric contraction. NT (10(-10)-10(-5) M) did not influence basal tension, frequency and peak phasic contraction of the whole guinea-pig portal vein (GPV) or its longitudinal strip (GLSPV). Intact reactivity of these preparations was derived from the contraction induced by carbamoylcholine or norepinephrine. In contrast, NT (6 X 10(-10)-6 X 10(-6) M) increased basal tension, frequency, and peak phasic contraction of the longitudinal strip of rat portal vein (RLSPV). Furthermore, NT induced a dose-dependent contraction in the terminal ileum of the guinea-pig (GTI) which was preceded by a transient relaxation. Comparing the effects obtained in GPV and GTI or found in RLSPV and GLSPV it is evident that the variable effects of NT depend on the location of smooth muscle and on animal species, respectively.

Animals↗

Characterization of tick antigens inducing host immune resistance. I. Immunization of guinea pigs with Amblyomma americanum-derived salivary gland extracts and identification of an important salivary gland protein antigen with guinea pig anti-tick antibodies.

Guinea pigs immunized by subcutaneous injection of an emulsion of incomplete Freund's adjuvant (IFA) containing tick salivary gland extract antigens (SGA) from partially fed female ticks expressed a significant level of tick rejection when challenged 17 days later. This level of tick rejection was similar to animals actively sensitized by tick feeding and challenged at the same time. SGA emulsified with complete Freund's adjuvant (CFA) or administered with saline was ineffective. However, ticks that fed on animals immunized with SGA+IFA or SGA+CFA expressed significant reductions in engorgement weight. SGA was active when prepared with or without protease inhibitors. The minimum effective immunizing dose of SGA was between 100 and 280 micrograms per animal. Extracts made from salivary gland-derived cement material (CA) from partially fed female ticks administered at 50 micrograms in IFA induced levels of tick rejection comparable to animals immunized with 280 micrograms of SGA+IFA. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS/PAGE) of 35S- and 125I-radiolabeled SGA and CA extracts immunoprecipitated by guinea pig anti-tick serum that transferred immune resistance demonstrated a unique protein of 20,000 m.w. Serum from animals immunized with SGA+IFA (successful immunization) recognized this same protein, whereas serum from animals immunized with SGA+CFA (unsuccessful immunization) did not. The results of this study suggest that a 20,000 m.w. protein derived from the tick salivary gland may be responsible for the induction and perhaps elicitation of host immune resistance responses to Amblyomma americanum ticks.

Animals↗

Characterization of two strains of selectively bred guinea-pigs. 3. Homocytotropic antibodies in guinea-pig lines with high or low susceptibility to respiratory anaphylaxis.

Two strains of guinea-pigs selectively bred for either high (IMM/S) or low (IMM/R) ovalbumin-induced respiratory anaphylaxis were examined for correlations between respiratory anaphylaxis and production of homocytotropic and passive hemagglutinating antibodies. The passive cutaneous anaphylaxis assay (PCA-assay) was used to test for the production of the three classes of homocytotropic antibodies, IgGla, IgGlb and IgE after immunization either by inhalation of the antigen or by intraperitoneal injection of the antigen adsorbed on aluminium hydroxide. IgE was not detected in any of the strains within the seven-week period following immunization. When immunization was performed by the inhalation technique, antibodies of the two subclasses IgGla and IgGlb as well as hemagglutinating antibodies were demonstrated in all sublines from IMM/S, while in sublines from IMM/R IgGla and IgGlb were never found, and hemagglutinating antibodies were only detected in small amounts or not at all. However, injection of the antigen with aluminium hydroxide as adjuvant resulted in production of hemagglutinating antibodies as well as IgGla and IgGlb in both IMM/S and IMM/R. When such animals were challenged by inhalation of the antigen, a severe respiratory anaphylactic response was induced in guinea-pigs from IMM/S only.

Adjuvants, Immunologic↗

Ocular and dermal delayed hypersensitivity reactions in guinea-pigs following infection with guinea-pig inclusion conjunctivitis agent (Chlamydia psittaci).

Guinea-pigs which had been sensitized to guinea-pig inclusion conjunctivitis agent by ocular infection, were given ocular and dermal challenges with a range of doses of agent. Ocular delayed-type hypersensitivity responses were elicited by a wide range of doses. Such responses were associated with the presence of chlamydial inclusions and inflammatory cells. The EID50 for ocular reinfection was only slightly higher than that for primary ocular infection. Dermal delayed-type hypersensitivity reactions were produced only when high doses of agent were used. The relevance of these observations to human chlamydial eye disease is discussed.

Animals↗

Identification and functional characterization of A1 and A2 adenosine receptors in the rat vas deferens: a comparison with A1 receptors in guinea pig left atrium and A2 receptors in guinea pig aorta.

This study was undertaken to characterize the adenosine receptors in the rat vas deferens. Because adenosine receptors have been well characterized in the cardiovascular system of the guinea pig, antagonist dissociation constants (pKB values) in the rat vas deferens were compared with those from the left atrium (A1) and the aorta (A2) of the guinea pig. The A1-selective agonists (+/-)-N6-endonorbornan-2-yl-5'-N-hydroxy ethylcarboxamidoadenosine (N-0723) and N6-cyclohexyladenosine (CHA) and the nonselective agonist 5'-N-ethylcarboxamidoadenosine (NECA) inhibited the electrically evoked contractions of both the vas deferens and left atrium with a potency order of N-0723 > NECA = CHA. The A2a-selective agonist 2-[p-(2-carboxyethyl)-phenethylamino]5'-N-ethylcarboxamidoadenosin e (CGS21680) was equipotent to NECA in the vas deferens but was 500-fold less potent than NECA in the left atrium. In the aorta only NECA was a potent agonist. The nonselective adenosine receptor antagonist 8-phenyltheophylline antagonized the responses in all three tissues with approximately equal potency (pKB approximately 6.6). In the rat vas deferens, the A1-selective antagonists (+/-)-N6-endonorboman-2-yl-9-methyladenine (N-0861) and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) were more potent at antagonizing the responses to A1-selective agonists (pKB approximately 8.8 and 6.4, respectively) than they were at antagonizing the responses to NECA and CGS21680 (pKB = 6.3 and < 5, respectively). However, in the left atrium, N-0861 (pKB = 6.2) and DPCPX (pKB = 8.9) were no more potent in antagonizing responses to the A1-selective agonists than they were in antagonizing responses to NECA.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenine↗

Intraadrenal steroid metabolism in the guinea pig: guinea pig adrenal microsomes metabolize androstenedione in a manner distinct from liver microsomes.

Several immunochemical homologs of hepatic cytochromes P450 (CYPs) capable of steroid catabolism have been identified in the guinea pig adrenal cortex. Their predominance in males suggests a role in sex-differentiated metabolism of androgens. Therefore, we examined the ability of microsomes from male guinea pig adrenals and liver to metabolize androstenedione. Microsomes were incubated in the presence of radiolabeled steroids, the products of the reaction extracted, separated by TLC, and visualized by autoradiography. Metabolites were identified by comigration with commercially available standards in several solvent systems, in one and two dimensional TLC. Microsomes from both tissues metabolized androstenedione. However, the products formed differed markedly in the two tissues. Liver microsomes formed one major metabolite, testosterone. It represented 85% of the metabolized androstenedione. 6 beta-Hydroxylated androstenedione and testosterone each comprised 3-4% of the liver metabolites. In addition, at least 10 other products were formed, but taken together they constituted less than 8% of the metabolized androdostenedione. Adrenal microsomes, on the other hand, produced several major metabolites: 16 alpha-, 16 beta-, and 6 beta-hydroxy-androstenedione, plus one unidentified product constituted 93% of the metabolized androstenedione. 16 alpha-Hydroxylation of androstenedione was 60 fold, 16 beta-hydroxylation 12 fold, and 6 beta-hydroxylation 2.5 fold greater in adrenal than in liver microsomes. The unidentified product, which was the least polar, was formed exclusively by adrenal microsomes. The hydroxylation reactions performed by adrenal tissue are consistent with the presence in adrenal microsomes of immunochemical homologues of members of the CYP1A, 2B, 2C and 3A families which have known steroid hydroxylation functions in liver. The Kms of the formation of 16 alpha-, 16 beta- and 6 beta-hydroxyandrostenedione by adrenal microsomes are in the range reported for steroid hydroxylation reactions in rat liver tissue. Their distinct values suggest that these hydroxylation steps are performed by different CYPs. However, assignment of site-specific steroid hydroxylation reactions to individual hepatic CYP homologs in the adrenal requires further investigation and is being pursued using combined techniques of biochemistry and molecular biology.

Adrenal Glands↗

A comparison of opioid peptide precursors in guinea pig, rat and bovine striata and guinea pig adrenal.

In this study guinea pig, rat and bovine striatal extracts have been shown to contain putative opioid peptide precursors in the molecular weight (Mr) range 2000-greater than 70000. The material eluting greater than 70 kDalton contained bioassayable opioid activity but not Met- or Leu-enkephalin-like immunoreactivities (ME-IR and LE-IR) and may represent a novel endogenous opioid-peptide precursor.

Adrenal Glands↗

Analysis of preformed xenoreactive antibodies in the discordant guinea pig to rat model using a guinea pig fibroblast-like cell line.

In the discordant guinea pig (gp) to rat model of xenotransplantation, circulating xenoreactive natural antibodies (XNA) recognizing gp antigens are usually determined by an ELISA using membrane extracts of gp platelets. We analysed the lung-derived, fibroblast-like cell line JH 4 to detect XNA by ELISA or immunoblot, which was compared to primary gp cells, i.e. platelets, liver- and spleen cells. All membrane extracts proved to be useful to detect rat XNA directed against gp antigens by ELISA. In general, IgM responses of Lewis or C6 deficient PVG rats (PVG/C6-) were higher as compared to IgG responses. However, we observed great inter-individual variabilities. The strongest IgM response of Lewis rat sera was observed when the JH 4 cell line or gp liver cells were used as antigen. JH 4 cells also showed the strongest xenoreactivity with sera from PVG/C6- rats. These data demonstrate that JH 4 cells prove useful as antigen source for XNA ELISA. In immunoblot, individual sera of the two different rat strains showed the same antigen patterns using a gp membrane extract of one particular cell type. However, the different gp cell types showed a distinct pattern of antigen expression. Whereas the JH 4 cells, platelets and spleen cells express xenoreactive proteins of the same size, a unique pattern of proteins was detected in liver cells.

Animals↗

Reduced congenital cytomegalovirus (CMV) infection after maternal immunization with a guinea pig CMV glycoprotein before gestational primary CMV infection in the guinea pig model.

This study evaluated the effects of subunit guinea pig (GP) cytomegalovirus (CMV) immunization on congenital infection. Two 25-micrograms doses of an abundant GPCMV glycoprotein, the gp60-90 complex, plus adjuvant to GPs before pregnancy produced virus-specific humoral (neutralizing and ELISA) and cellular (proliferative and delayed type hypersensitivity) responses. Viral challenge before midgestation resulted in shorter maternal viremia in immunized than in unimmunized dams (3 vs. 17 days). Litters of immunized dams had reduced organ involvement and rates of congenital infection (48% vs. 18%) and increased mean birth weights (74 vs. 99 g). Amplification of DNA extracted from pup blood or organs with primers from the gB gene of GPCMV revealed congenital infection in some pups without detectable CMV by classic culture techniques. These data suggest that induction of preconception immunity to CMV by vaccine could be useful in reducing both the incidence and severity of congenital CMV disease.

Animals↗

Iodine deficiency in Papua New Guinea (sub-clinical iodine deficiency and salt iodization in the highlands of Papua New Guinea).

Data on the status about iodine nutrition in children in Papua New Guinea (PNG) are scarce. This study attempts to determine the mean daily per capita consumption of salt, the iodine content of salt in the households and retail shops and the urinary iodine concentration in children (6-12 years) in Hella Region, Southern Highland Province (SHP), PNG. The mean daily consumption of salt was 2.62 +/- 1.29 g. The iodine content of salt was >30 p.p.m. in 95 per cent of households and 100 per cent of retail shops. The median urinary iodine concentration of 48.0 mg/l for all the children indicates moderate iodine deficiency. The median urinary iodine concentrations for the male (67.0 mg/l) and female (44.0 mg/l) children indicate mild and moderate iodine deficiency, respectively. 68.42 per cent of the male and 81.82 per cent of the female children have urinary iodine concentration <100 mg/l, indicating that iodine deficiency is a potential public health problem in the Hella region. These results indicate a need for further assessment of the implementation of the universal salt iodization strategy for the elimination of iodine deficiency in the SHP, PNG.

Child↗

Generalized rash induced by sulfhydryl drug in guinea pigs--a comparative study on it with cephalothin-induced rash in guinea pigs.

Adverse reactions produced by sulfhydryl compounds with active thiol groups have generally formed a distinctive pattern in man when they are viewed as a class. It has been reported that cutaneous SH-induced drug eruptions have a wide variety of clinical presentations; histologically, they show a pattern of eosinophilic necrosis and/or satellite necrosis similar to that seen in cutaneous graft vs host reactions. In the present experiments, guinea pigs were sensitized with cephalothin (CET) and thiol compounds such as tiopronin (TP), D-penicillamine, gold sodium thiomalate or thiomalate, using a method similar to that described previously. In lymphocyte stimulation tests, lymph node cells from the sensitized animals responded positively to spleen cells pulsed with each thiol compound. Intracutaneous tests revealed some positive reactions to each thiol compound; there was a tendency to produce a tuberculin type reaction with indurated erythema rather than the Jones-Mote type seen in CET-induced reactions. The dose-requirements for positive intracutaneous tests and generalized rash (GR) due to thiol compounds were lower than for CET, which required relatively large doses. Histologically, infiltration of basophilic cells was prominent in the skin lesions induced by intracutaneous tests with CET and in those of CET-induced GR. On the other hand, intracutaneous tests with TP following the induction of TP-induced GR revealed eosinophilic degeneration of epidermal cells, which was similar to the eosinophilic necrosis seen in cutaneous GVHR. Intracutaneous tests after the induction of CET-GR did not show any eosinophilic changes in the epidermal cells. These findings are reminiscent of the characteristics of eruptions induced by thiol compounds in man, which differ from the eruptions induced by CET.

Animals↗

Gluconeogenesis in the guinea pig. Effect of glucagon on gluconeogenesis from lactate by isolated perfused guinea-pig liver.

Gluconeogenesis was stimulated by glucagon in fed but not fasted isolated perfused guinea pig livers. Both the amount and the rate of incorporation of radioactivity into glucose from L-[U-14C]lactate were increased in fed livers by the addition of glucagon to the perfusate. The glucagon-stimulated increase in gluconeogenesis was accompanied by an increase in oxygen consumption, an increase in the amount of lactate carbon converted to glucose and a decrease in the amount of lactate carbon converted to CO2. The results are interpreted to indicate that glucagon affects gluconeogenesis from lactate in fed livers by redirecting the fate of substrate from other products toward glucose.

Animals↗

Plasma lipoproteins of leukemic guinea pigs (L2C) can regulate cholesterol biosynthesis by lymphocytes of normal guinea pigs. A comparative study of plasma lipoproteins of normal and neoplastic animals.

The defect of regulation of cholesterol biosynthesis by leukemic (L2C) guinea pig lymphocytes is not a consequence of serum lipoprotein modifications which would make them unable to participate in the regulatory process. Low density lipoprotein of leukemic animals, in parallel to normal low density lipoprotein, can inhibit the cholesterol biosynthesis by normal cells. Surprisingly, very low density lipoprotein of leukemic animals have the same inhibitory property. Analyses of serum of leukemic animals showed a larger amount of the different lipoprotein fractions (+323% very low density, +27% low density lipoproteins, the high density lipoprotein staying undetectable in control and leukemic sera) than in normal serum. L2C leukemia produces low density lipoprotein slightly richer in unesterified cholesterol and very low density lipoprotein markedly modified by an increased proportion of unesterified cholesterol, phospholipids and apoprotein B. The inhibitory power of leukemic very low density lipoprotein is discussed by analogy with corresponding power of normal low density lipoprotein which can operate either by the way of binding to the low density lipoprotein receptor or by exchange of unesterified cholesterol between the lipoprotein and the cell.

Animals↗