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Penetration of glutaric dialdehyde into human dentine as measured by changes in dentine microhardness and dentine dimensions.

Microhardness indentation lengths were measured on human dentine sections unfixed and fixed with glutaric dialdehyde (GDA) at pH 7. Fixed dentine was found to be softer than unfixed dentine. Indentation length increased by approximately 10% after fixation in a buffered 5% GDA solution at pH 7. Using this effect the variation of indentation length in the direction of the penetrating GDA as a function of fixation time was measured. Describing the penetration in the mineralized matrix as diffusion in a plane sheet, a diffusion coefficient of 0.5.10(-12); m2/s was calculated. Dimensional changes of the dentine due to fixation were also measured. Dentine was found to expand in both the direction parallel (approximately 0.4%) and perpendicular (approximately 2%) to the tubules.

Buffers↗

The in vitro morphological effects of some current pre-treatments on dentin surface: a SEM evaluation.

This in vitro study morphologically evaluated the effect of some current surface pre-treatments on dentin, using scanning electron microscopy, and related these morphological alterations to clinical implications. The labial surfaces of 30 bovine lower incisors were ground to obtain a flat dentin surface and were finished with 600-grit SiC paper to produce standardized smear layers. The teeth were randomly divided into six groups of five each. Group 1 was the control group, smear layer covered dentin; Group 2 was etched with 37% phosphoric acid (PA) for 15 seconds; Group 3, 37% PA for 15 seconds, followed by 10% NaOCl for 60 seconds; Group 4, 10% NaOCl for 60 seconds; Group 5, a self-etching primer (Clearfil SE Bond, CSEB-primer) was applied for 20 seconds; Group 6, CSEB-primer for 20 seconds, followed by NaOCl for 60 seconds. The specimens were fixed, dehydrated, dried and analyzed by SEM. Treatment with 37% PA removed the smear layer, funneled the tubules and resulted in a collagen-rich surface which appeared to have collapsed in its outermost part, producing a dense surface layer covered with silica particles. When 37% PA treatment was followed by 10% NaOCl, the collagen network was removed to reveal an eroded, rough mineral surface with numerous lateral branches and larger than normal tubular orifices. The action of 10% NaOCl on the smear layer-covered dentin showed no significant alteration in surface morphology. The treatment with CSEB-primer dissolved the smear layer but only partially dissolved the smear plugs. The tubules did not present the typical funnel shape seen following PA treatment. These morphological aspects on dentin surface must influence bonding results. The dentin surface alterations produced by PA appeared to be a very severe demineralization pattern, quite irregular and less permeable to monomer infiltration, while the surface provided by the self-etching primer appeared to be a more uniform, less porous surface, and the association with simultaneous monomer infiltration may reduce the occurrence of mistakes in clinical bonding procedures.

Acid Etching, Dental↗

The effect of an antimicrobial releasing varnish on root demineralisation in situ. The influence of the demineralisation period.

Caries in exposed root surfaces is becoming a significant problem in dentistry. Varnish applications have interesting preventive possibilities against root caries. The effect of such a varnish, containing two antimicrobials, was studied on the demineralisation of roots with a twofold aim. Firstly, the efficacy of the varnish was investigated in situ as a function of the demineralisation period. Ten participants wore sound intact roots treated with the varnish in an intra oral appliance for 2, 4 and 6 weeks. Secondly, the effect of the varnish on root demineralisation after removal of the varnish was measured. The results showed that the reduction, in demineralization of about 80% after 2 weeks, decreased in magnitude with the length of the in situ demineralisation period. After 4 weeks there was no longer statistically significant difference compared with untreated roots. The acid attack by plaque was, however, substantial in this model; after 6 weeks in situ untreated roots were so strongly demineralised that microradiography was no longer possible anymore. In the second experiment in which the varnish was removed 2 days after application there was a trend toward a reduction of about 30% in root demineralisation after 2 weeks. This was not statistically significant when compared with no application.

Adult↗

Optimal citric acid concentration for dentinal demineralization.

A number of acids have been shown to have a peak concentration above which the rate of enamel demineralization diminishes. Citric acid, used to demineralize dentin in periodontal regenerative procedures, is one such acid. The purpose of this study was to ascertain if there were an optimal concentration of citric acid with which to demineralize dentin. Various concentrations of citric acid were applied to bovine dentin and then examined with atomic absorption spectrophotometry to measure the amount of calcium dissolved in each concentration. The ppm calcium/% citric acid solution rose from 0.38 +/- 0.38 ppm/0% (control) to a peak of 7.54 +/- 2.26 ppm/24%, then fell to 2.43 +/- 0.59 ppm/80%, suggesting that there may be a peak concentration beyond which effective dentinal demineralization diminishes. The peak solution was pH = 1.42.

Animals↗

Effect of fluoride release from a fluoride-containing composite resin on secondary caries: an in vitro study.

The effect of fluoride, released from a fluoride-containing composite resin, on secondary caries was investigated by quantitative microradiography. In all teeth investigated, less demineralization was found near the composite resin than was found at a distance of a few millimeters from the material after an acid attack. The presence of the fluoride-releasing composite resin reduced the lesion depth measured after an acid attack by about 35%. Therefore, in vivo, a reduction of recurrent caries may be expected around fluoride-containing composite resins.

Adult↗

Basic study of fluoride-releasing adhesive resin for prevention of root caries: 2. Enhancement of acid resistance of the dentin.

The human root dentin was covered with a fluoride-releasing resin (F-resin) and incubated for various periods up to 180 days in phosphate buffer. After removal of the F-resin, resistance of the dentin to decalcification by acetic acid--sodium acetate buffer was assessed by measuring both the amount of calcium dissolved during decalcification and the knoop hardness of dentin after decalcification. The acid resistance progressively increased for 90 days when assessed by measurement of calcium dissolution and for 30 days by measurement of the knoop hardness. The percent inhibition of calcium dissolution achieved by 30 days of incubation was about 90% of the maximal inhibition obtained by incubation in concentrated sodium fluoride solution (2.4 x 10(-2) M). The enhancement of acid resistance was not induced by the resin infiltrated layer of the surface dentin but by the fluoride released from the F-resin into the dentin.

Acetates↗