Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Decalcification Technique”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources
PubMed1993

The innervation of the human larynx.

OBJECTIVE: To investigate the gross anatomy of the recurrent and superior laryngeal nerves (RLNs and SLNs) in 10 human larynges. METHODS: Whole larynges were processed to clear all soft tissue while leaving nerves stained. Then the main laryngeal nerves and the muscles they innervate were dissected and analyzed. RESULTS: It was found that in all larynges the RLNs and SLNs are connected by nerve branches other than Galen's anastomosis. The most consistent connection is in the interarytenoid muscle, where RLNs and internal SLNs combine in a neural plexus. A less consistent connection occurs in the piriform fossa, where a continuation of the external SLN passes from the cricothyroid muscle to the thyroarytenoid muscle. CONCLUSION: Based on these findings it is proposed that there are significant neural connections between the RLN and SLN systems. In addition, limited cross-innervation is seen from side to side in the area of the interarytenoid muscle. Other findings concern the innervation patterns within the laryngeal muscles. The posterior cricoarytenoid, cricothyroid, and thyroarytenoid muscles all appear to be composed of separate bellies based on the configuration of their nerve supply. Most notable is the region of the thyroarytenoid muscle at the vocal cord margin that is innervated by a nerve plexus of extreme complexity. The details of the innervation patterns suggest functional differences within and between laryngeal muscles.

Acetates↗
PubMed1996

Working experience with a new vacuum-accelerated microwave histoprocessor.

Methods of microwave and vacuum-accelerated fixation, dehydration, and paraffin embedding are described, using a new type of vacuum and temperature stabilizable microwave histoprocessor: MFX-800. The whole histoprocessing cycle lasts 3.5-5.5 h, depending on the thickness of the tissue blocks. Well-preserved structural detail, intense staining, and good antigen preservation were achieved with various tissues. This new histoprocessing facility is recommended for routine pathology laboratories in speeding up their processing procedures.

Decalcification Technique↗
PubMed1996

Light and electron microscopic studies of bone-titanium interface in the tibiae of young and mature rats.

Bone-titanium contact was examined in young and mature rats on various days after insertion of pure titanium into the tibia. Under light microscopy, on the 14th day, lamellar mature bone was initially formed, and was seen to make direct contact with the titanium in both groups. In young rats on the 28th day, bone-titanium contact was greater than that in mature animals. On 1-micron sections, an amorphous zone 0.5-1.0 micron thick was found around the titanium, and a slender cell layer lay parallel to the implant, forming the superficial layer of the amorphous zone. Ultrastructurally, these slender cells were identified as osteoblastlike cells and made direct contact with the implant via a 20-50-nm thin amorphous zone. Below this cell layer, a collagen-containing, poorly mineralized zone was present and bordered by lamellar bone with a lamina limitans-like structure. However, this cell layer was absent in places, and therefore the thick amorphous zone without slender cell layer consisted ultrastructurally of a 20-50-nm thin amorphous zone and a poorly mineralized zone bordered by the lamellar bone. Sometimes this poorly mineralized zone was absent, and in such cases, the lamellar bone contacted the titanium by the thin amorphous zone formed on the lamina limitans-like structure. Thus, although bone was seen to make contact with the titanium implant, ultrastructurally a 20-50-nm thin amorphous zone, a slender cell layer, and/or a poorly mineralized zone were interposed between the bone and titanium.

Animals↗
PubMed1975

Correspondence of silver nitrate staining patterns in decalcified bone withe the microradiographic image.

A procedure for differential staining of decalcified bone with silver nitrate showed major histological features which appeared to correspond closely to microradiographic images. The extent to which this is actually the case was investigated directly by preparing microradiographs of ground sections of baboon and dog radii and then decalcifying and staining the same sections. The many detailed similarities indicate that this staining procedure is a useful adjunct to microradiography. Thus, poorly mineralized osteons or layers of circumferential lamellae are darker stained by silver nitrate, and the variably mineralized layers of circumferential lamellae are closely duplicated by light and dark bands in the stained sections. These similarities imply that there is a relationship between the mineral density of bone and some condition of the organic matrix which is probably related to maturation changes in the collagen.

Age Factors↗
PubMed1979

Structure of Retzius lines in partially demineralized human enamel.

Human third molars were partially demineralized in an acid-alcohol solution and embedded in Epon 812. Six-micron sections were cut from regions of the cervical enamel exhibiting prominent Retzius lines. The plastic was removed from the specimens by microincineration and were examined with the scanning electron microscope. The most prominent structural feature seen along the Retzius lines was the cervical translocation of some of the prisms. The scanning electron microscopic images also suggested that prisms were translocating in the transverse plane of the tooth. A series of pores, which appeared to be empty, were observed in association with the translocations occurring along the Retzius lines.

Acids↗
PubMedDate not supplied

Horizontal splitting in patellar articular cartilage.

Horizontal splits at the interface between the uncalcified and the calcified layers of the articular cartilage have been found in 31 of 50 transverse slabs of left patellae from a random series of 50 necropsy subjects aged 18 to 96 years, Evidence is presented that the splits are not due to autolysis nor to technical artefact, and it is here suggested that they result from shearing damage at the uncalcified-calcified cartilage interface during life.

Adolescent↗