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Conductometric properties of human erythrocyte membranes: dependence on haematocrit and alkali metal ions of the suspending medium.

The electrical properties of the cytoplasmatic membrane of human erythrocyte cells have been evaluated by means of dielectric spectroscopy measurements in the radiowave frequency range, using the so-called "suspension method". Measurements have been carried out at different volume fractions of the corpuscular phase (the cell haematocrit) in order to investigate the influence of the cell-cell interactions on the electrical parameters (the membrane permittivity epsilon and the membrane conductivity sigma) of the cell membrane and a set of new values are proposed. Moreover, the influence of different alkali metal ions (Na+, K+, Cs+, Li+) on the ion permeation properties of the membrane are investigated and the structural alterations in the membrane organized briefly discussed.

Adult↗

An electrochemical assay for the characterization of redox proteins from biological electron transfer chains.

A sensitive and quick assay for redox proteins based on electrochemical titrations in a thin-layer electrochemical cell is described. Using a combination of modified-electrode and "mediator-enhanced" electrochemistry, equilibration of the cell volume (4 microliters) with the applied potential allows series of spectra as a function of the potential to be recorded rapidly. A complete redox titration between +500 and -600 mV (vs Ag/AgCl/3 M KCl) in 30-mV intervals takes approximately 2 h. The detection limit of the assay, evaluated for cytochrome c at the alpha-band absorption, is quoted to approximately 100 pmol. The use of this redox assay for the detection of redox-active contaminants in biochemical preparations, for the determination of midpoint potentials of redox enzymes, and for the characterization of complex membrane-bound or soluble redox systems is described.

Bacteria↗