Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “COMPLEMENT FIXATION TESTS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources
PubMed1997

Serodiagnosis of Babesia equi infection--a comparison of Dot-ELISA, complement fixation test and capillary tube agglutination test.

The present study aimed to develop Dot-ELISA, complement fixation test (CFT) and capillary tube agglutination test (CAT) for serodiagnosis of Babesia equi infection and to compare their sensitivity with each other. For this study, sequential serum samples were collected from four donkeys experimentally infected with B. equi up to 90 days post infection (P.I.). B. equi antigen was prepared from the blood of a donkey showing more than 80% parasitaemia. Dot-ELISA, CF and CA tests were standardized as per the standard method. While performing CFT, it was observed that CFT standardized for the donkey system could not be applied to the horse system, and different units of the same antigen and complement were required for each. Dot-ELISA detected antibodies from 3-6 days P.I. onwards, whereas CF and CA tests could detect antibodies from the 6th day P.I. indicating that Dot-ELISA is the more sensitive. The efficacy of these three tests was also determined by testing 211 field serum samples of apparently healthy horses. By Dot-ELISA 49.76%, by CAT 42.18% and by CFT 27.86% of the serum samples were found positive. As the results of Dot-ELISA and CAT are comparable, it is proposed that CAT may be used as a screening test.

Agglutination Tests↗
PubMed1986

Comparison of MYCOPLASMELISA with complement fixation test for measurement of antibodies to Mycoplasma pneumoniae.

The MYCOPLASMELISA test kit was compared with the complement fixation test for detection of Mycoplasma pneumoniae antibody in single sera and evaluation of acute- and convalescent-phase serum pairs for significant rises in antibody titers. Agreement between the two assays was 96.6% (85 of 88) for antibody detection and 93.9% (92 of 98) for evaluation of paired sera. Sensitivity and specificity of MYCOPLASMELISA, relative to complement fixation, for the serodiagnosis of recent M. pneumoniae infection was 92.2% (59 of 64) and 95% (19 of 20), respectively.

Antibodies, Bacterial↗
PubMed1966

Complement-fixation tests with cell lines derived from Burkitt's lymphoma and acute leukemias.

Armstrong, Donald (Children's Hospital of Philadelphia, Philadelphia, Pa.), Gertrude Henle, and Werner Henle. Complement-fixation tests with cell lines derived from Burkitt's lymphoma and acute leukemias. J. Bacteriol. 91:1257-1262. 1966.-Cells of various lines isolated from Burkitt's lymphomas and acute leukemias and disintegrated by freezing and thawing or sonic treatment were found to react in complement-fixation tests with a considerable proportion of human sera. At least 10(7) cells per milliliter were required for antigenic activity. All but one of 13 sera from Burkitt lymphoma patients were positive, with titers ranging from 1:8 to 1:320. About 20% of sera from American children and 60% of sera from adults, regardless of diagnosis, showed titers in a similar range. Sera giving positive tests with one of the neoplastic white cell antigens usually reacted also with many if not all of the others, but rarely with antigens derived from normal peripheral leukocyte cultures and not at all with HeLa or other human nonleukocytic cells. Various observations indicate that the complement-fixation test measures mainly antigens which are different from those detected by immunofluorescence. The nature of the reactions described remains obscure.

Adenocarcinoma↗
PubMed1989

Development of a monoclonal antibody for use as an amboceptor in complement fixation tests.

A monoclonal antibody for use as a haemolytic amboceptor in complement fixation tests was developed. The monoclonal antibody selected was investigated in parallel with conventional rabbit amboceptor. The results with the monoclonal amboceptor were at least as good as with the rabbit amboceptor. Production of the monoclonal amboceptor was attained provided that hybridoma growth factor was added to the culture medium.

Animals↗
PubMed1977

Single inoculation immune hamster sera for typing California group arboviruses by the complement-fixation test.

Eight reference California group viruses of North America were typed using sera of immune hamsters bled 21 days after a single inoculation. The complement-fixation test reactions were relatively specific, although only 2-fold differences were observed reciprocally with the closely-related La Crosse and snowshoe hare viruses. Hamster serum taken 10 days post inoculation was more specific than a 21-day serum. Sepcificity after second inoculation was lost with some antigens. Jamestown Canyon and South River viruses were identical by complement-fixation test and showed minor differences in the plaque reduction neutralization test.

Animals↗
PubMed1995

Diagnosis of American trypanosomiasis (Chagas' disease) by the new complement fixation test.

A new immunodiagnostic method of complement fixation was used for serodiagnosis of American trypanosomiasis; 92% sensitivity and 99% specificity were obtained, for an overall accuracy of 97%. This test can be used in field studies, obviating the use of most laboratory equipment and imported reagents; places where economic limitations hinder the use of other immunodiagnostic procedures; and in association with other tests for confirmation of the diagnosis.

Animals↗
PubMed1992

Evaluation of a commercially available complement fixation test for diagnosis of Helicobacter pylori infection and for follow-up after antimicrobial therapy.

Commercially available complement fixation test reagents (Institute Virion Ltd., Rüschlikon, Zurich, Switzerland) available in package format were evaluated for the serodiagnosis of Helicobacter pylori infection. The assay was compared with bacterial culture and histological Giemsa stain of gastric biopsy specimens obtained from 930 patients of different ages and from different ethnic groups, with a variety of upper gastrointestinal tract symptoms. The prevalence, sensitivity, specificity, and positive and negative predictive values, respectively, were 35, 71, 90, 80, and 85% for Belgian patients aged 40 years or younger, 50, 81, 93, 92, and 83% for Belgian patients older than 40 years, and 83, 83, 79, 95, and 48% for Mediterranean patients. Using 645 serum specimens from 226 patients, we also evaluated the complement fixation test for its ability to monitor the eradication of H. pylori following antimicrobial therapy. Overall, H. pylori was eradicated from 122 patients while 104 patients remained infected with the organism. A significant decrease in antibody levels was observed 3 to 6 months after the end of therapy in the group of patients from whom H. pylori was eradicated.

Adolescent↗
PubMed1968

Advantage of a routine Reiter protein complement-fixation test in the serodiagnosis of syphilis in pregnancy.

One hundred and twenty-seven (0.3%) reactive serological tests for syphilis were obtained by routine examination of sera from 35,912 antenatal patients.Forty-eight (38%) of these reactive sera were associated with reactive fluorescent treponemal antibody and Treponema pallidum immobilization results and these patients were therefore considered to show serological evidence of treponemal infection. Sera from 13 of these 48 patients (27%) gave reactive Reiter protein complement-fixation tests in the absence of detected ;reagin' antibody. Seventy-nine patients showed non-specific biological false positive reactions to the routine tests. Following clinical assessment of these serologically reactive patients, approximately one in 100 immigrant and one in 2,500 non-immigrant pregnant women examined in this series were treated with penicillin; this represented just over half the number of patients with serological evidence of a treponemal infection, a ratio which was similar for immigrant and native born women. Yaws was the likely treponemal infection in the majority of infected immigrants. The significance of the serological results and the advantages of the Reiter protein complement-fixation test are discussed.

Complement Fixation Tests↗
PubMed1982

Comparison of the complement fixation test and the indirect hemolysis test for cattle vaccinated and infected with Brucella abortus.

The complement fixation test (CFT) and indirect hemolysis test (IHLT) were applied to sera collected from 60 cattle challenged with Brucella abortus 544. Of the 60 cattle, 48 were vaccinated with either B. abortus 19 or B. abortus 45/20 as calves or as adults. The remaining 12 cattle were not vaccinated. Of the 27 sera from cattle found to be infected, 9 showed aberrant reactions to the CFT. The advantages of the IHLT for these cattle were as follows. After challenge, the titers to the IHLT became positive earlier than or at the same time as the titers to the CFT, they persisted longer than the titers to the CFT, and they failed to show prozone reactions, which are a problem with the CFT. An additional advantage was that before challenge and after vaccination with strain 19, the titers to the IHLT rose later and declined earlier than the titers to the CFT. We concluded that the CFT used in conjunction with the IHLT improves the detection of infected cattle.

Animals↗
PubMed1980

A complement fixation test for antigens of and antibodies to malignant catarrhal fever virus.

A complement fixation test was developed for the study of malignant catarrhal fever (MCF). A clarified ultrasonicate of cultured cells infected with MCF virus was used as antigen and detected increasing levels of complement fixing antibody in 12 out of 14 and four out of nine infected cattle and rabbits respectively. Titres greater than or equal 1:64 were found in hyperimmune sera from three out of four cattle, five out of five rabbits and in eight out of 143 wildebeest. No antibody was detected in 310 normal cattle sera from areas of Kenya with endemic MCF. The test did not detect viral antigens in a number of infected tissues or increased anticomplementary activity in sera from sick animals.

Animals↗
PubMed1981

Comparative titration of Anaplasma marginale antibodies by card agglutination and complement-fixation tests.

The card agglutination test for anaplasmosis was improved so that bovine serum could be titrated for Anaplasma antibodies. The modified test was validated when its results on 1 set of serum samples were compared with the results furnished by the complement-fixation test on the same set. The samples tested were from cows inoculated with an Anaplasma vaccine or with either 1 of 2 virulent strains of Anaplasma marginale. A correlation coefficient of 0.60 was calculated between the values of the titers furnished by the 2 tests, and regression equations were calculated and used to estimate the serum titer in 1 test from a titer measured in the other. The results indicate that the simple card agglutination test can yield titers comparable with those of the complement-fixation test.

Agglutination Tests↗
PubMed1975

[The complement fixation test with BCG in the diagnosis of leishmaniasis].

The utility of complement-fixation test with an antigen from commercially lyophilized BCG was investigated in the diagnosis of leishmaniasis. The following results were obtained: Visceral leishmaniasis: 4 positivity on 5 cases; Oriental sore: 3 positivity on 6 cases; Canine leishmaniasis: 6 positivity, at high level, and 2 negativity; Other diseases: 5 positivity, on 17 sera of tbc; No positivity in sera from patients with various diseases, in control and in normal dogs. The results are compared with those quoted in medical literature and discussed. The meaning of the reaction is also briefly discussed, and the hypothesis of the presence of partigens between BCG and leishmania, or presence of autoantibodies is prospected.

Animals↗