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Biotechnology awareness study, Part 1: Where scientists get their information.

A model study, funded by the National Library of Medicine (NLM) and conducted by the Southeastern/Atlantic Regional Medical Library (RML) and the University of Maryland Health Sciences Library, attempted to assess the information needs of researchers in the developing field of biotechnology and to determine the resources available to meet those needs in major academic health sciences centers. Nine medical schools in RML Region 2 were selected to participate in a biotechnology awareness study. A survey was conducted of the nine medical school libraries to assess their support of biotechnology research. To identify the information needs of scientists engaged in biotechnology-related research at the schools, a written survey was sent to the deans of the nine institutions and selected scientists they had identified. This was followed by individual, in-depth interviews with both the deans and scientists surveyed. In general, scientists obtained information from three major sources: their own experiments, personal communication with other scientists, and textual material (print or electronic). For textual information, most study participants relied on personal journal subscriptions. Tangential journals were scanned in the department's library. Only a few of these scientists came to the health sciences library on a regular basis. Further, the study found that personal computers have had a major impact on how biotechnologists get and use information. Implications of these findings for libraries and librarians are discussed.

Awareness↗

[Farmers in biotechnology].

Biotechnology experienced a stormy development during the past decade. The development of r-DNA technology was of major importance both to the world of science and to that of commerce. Biotechnological developments in the field of veterinary medicine will be commented upon and illustrated in a number of instances in which animals are central. Products which could so far only be obtained from animals, can be isolated from microorganisms on an unlimited scale today. The essential advantages of the biotechnological production of these products will be illustrated in the examples. In addition, it will be possible to use a large number of other products in the field of veterinary science. Both the current results of biotechnological studies and the rapidly increasing prospects for the future will be elaborated.

Agriculture↗

['New look' biotechnology].

The pursuit of biotechnology is to use living organisms to obtain particular products. Biotechnology, 'New Look', takes an essential step further in this pursuit:.....production is based on manipulating the genome: genototechnology! The production of monoclonal antibodies starts by fusing lymphocytes and myeloma cells. The lymphocytes bring in their programme in this process, and the myeloma cells (tumour cells) will ensure permanent mitotic activity. In recombinant DNA technology, 'foreign' DNA is incorporated into an existing genome so that the (bacterial) cell starts to produce products unfamiliar to this cell. An outstanding feature of biotechnology, 'New Look', consists in the fact that the instrumentation required frequently is biotechnological itself.

Animals↗

Prospects and problems of the new biotechnologies in the dairy industry.

Biotechnology and genetic engineering are defined and their applications in the dairy industry described for pasture and animal improvement, and for dairy product processing in the factory. Problems in establishing new biotechnologies in the dairy industry and the regulatory hurdles that have to be overcome are discussed. Gene probes and monoclonal antibodies as diagnostics, bovine somatotropin to increase milk production, recombinant DNA-rennin in cheesemaking, recombinant-DNA starter cultures, and enzymes to convert lactose to oligosaccharides are used as examples relevant to the dairy industry. It is advocated that the dairy industry develop a biotechnology policy and an education programme, and actively consider joint ventures with dedicated biotechnology companies.

Animals↗

[Abstract or concrete: the importance of biotechnological descriptions in its evaluation].

Biotechnology is an environmental issue that can be evaluated with regard to the impact of the technology "as such" or with regard to specific products. We assume that the concreteness of presentations of biotechnology has an influence on evaluations of risks and benefits. We conducted two experimental studies. In Study 1, 99 subjects evaluated the strength and importance of pro and contra arguments that were presented in short texts on biotechnology. In study 2, 141 subjects were given short texts on biotechnology as well as 15 scales measuring evaluations of risks and chances. Datareveal an influence of the factor "concreteness" on evaluations of pro arguments and benefits as well as on evaluations of contra arguments and risks. But results point to an interaction between levels of concreteness of descriptions and their specific contents: in the pharmaceutical domain concrete descriptions are evaluated more positively than abstract descriptions, in the agricultural domain concrete descriptions are evaluated more negatively.

Adult↗

Perception of risks and benefits of in vitro fertilization, genetic engineering and biotechnology.

The use of new biotechnology in medicine has become an everyday experience, but many people still express concern about biotechnology. Concerns are evoked particularly by the phrases genetic engineering and in vitro fertilization (IVF), and these concerns persist despite more than a decade of their use in medicine. Mailed nationwide opinion surveys on attitudes to biotechnology were conducted in Japan, among samples of the public (N = 551), high school biology teachers (N = 228), scientists (N = 555) and nurses (N = 301). People do see more benefits coming from science than harm when balanced against the risks. There were especially mixed perceptions of benefit and risk about IVF and genetic engineering, and a relatively high degree of worry compared to other developments of science and technology. A discussion of assisted reproductive technologies and surrogacy in Japan is also made. The opinions of people in Japan were compared to the results of previous surveys conducted in Japan, and international surveys conducted in Australia, China, Europe, New Zealand, U.K. and U.S.A. Japanese have a very high awareness of biotechnology, 97% saying that they had heard of the word. They also have a high level of awareness of IVF and genetic engineering. Genetic engineering was said to be a worthwhile research area for Japan by 76%, while 58% perceived research on IVF as being worthwhile, however 61% were worried about research on IVF or genetic engineering. Japanese expressed more concern about IVF and genetic engineering than New Zealanders. The major reason cited for rejection of genetic manipulation research in Japan and New Zealand was that it was seen as interfering with nature, playing God or as unethical. The emotions concerning these technologies are complex, and we should avoid using simplistic public opinion data as measures of public perceptions. The level of concern expressed by scientists and teachers in Japan suggest that public education "technology promotion campaigns" will not reduce concern about science and technology. Such concern should be valued as discretion that is basic to increasing the bioethical maturity of a society, rather than being feared.

Adult↗

Biotechnology as an intellectual property.

Recent advances in biotechnology have created many public policy and legal issues, one of the most significant of which is the treatment of biotechnological industrial products, particularly under the patent system. Patents represent one of several types of intellectual property; their ownership confers the right to exclude others from benefitting from the tangible products of a proprietary subject matter. Intellectual property law and its protections will play a major role in the rate at which biotechnology develops in the United States. In this article biotechnological intellectual property issues are reviewed in the context of their underlying legal requirements. The implications of other factors, such as international competition, research funding, and gene ownership, are also considered.

Biomedical Research↗

Towards harmonization: the European biotechnology patent directive.

Having first been proposed over a decade ago to harmonize European patent law for the benefit of Europe's fledgling biotechnology industry, the Biotechnology Patent Directive underwent a somewhat tortured introduction to adolescence when its adoption was challenged by a legal annulment action from the Governments of the Netherlands, Italy and Norway. This article explores the nature of the legal challenge to the Directive, which to an extent typify European concerns about biotechnological inventions, particularly the "patenting of life."

Animals↗

The Biotechnology Facility for International Space Station.

The primary mission of the Cellular Biotechnology Program is to advance microgravity as a tool in basic and applied cell biology. The microgravity environment can be used to study fundamental principles of cell biology and to achieve specific applications such as tissue engineering. The Biotechnology Facility (BTF) will provide a state-of-the-art facility to perform cellular biotechnology research onboard the International Space Station (ISS). The BTF will support continuous operation, which will allow performance of long-duration experiments and will significantly increase the on-orbit science throughput.

Animals↗

The impact of industrial biotechnology.

In this review, the impact of industrial (or "white") biotechnology can have on our society and economy is discussed. An overview is given of industrial biotechnology and its applications in a number of product categories ranging from food ingredients, vitamins, bio-colorants, solvents, plastics and biofuels. The use of fossil resources is compared with renewable resources as the preferred feedstock for industrial biotechnology. A brief discussion is also given of the expected changes in society and technology, ranging from the shift in the supply of resources, the growing need for efficiency and sustainability of the production systems, changing consumer perception and behaviour and changing agricultural systems and practices. Many of these changes are expected to speed up the transition from a fossil-based to a bio-based economy and society.

Biotechnology↗

Environmental biotechnology in China: progress and prospect.

Biotechnology could be widely applied in the technological development of environmental protection. This report gives a brief Chinese review on the major progress of environmental biotechnology in the following fields: Monitoring technology and treatment of dioxin-like chemicals and cyanobacterial toxins, biofiltration for air pollution control, solid waste treatment and reutilization, bioremediation of soil pollution, risk assessment and control of endocrine disturbing substances in environment, wastewater treatment, clean production and recycling economy. The effect of government policy and fund on the progress of environmental biotechnology is also discussed.

Biotechnology↗

Downstream processing in marine biotechnology.

Downstream processing is one of the most underestimated steps in bioprocesses and this is not only the case in marine biotechnology. However, it is well known, especially in the pharmaceutical industry, that downstreaming is the most expensive and unfortunately the most ineffective part of a bioprocess. Thus, one might assume that new developments are widely described in the literature. Unfortunately this is not the case. Only a few working groups focus on new and more effective procedures to separate products from marine organisms. A major characteristic of marine biotechnology is the wide variety of products. Due to this variety a broad spectrum of separation techniques must be applied. In this chapter we will give an overview of existing general techniques for downstream processing which are suitable for marine bioprocesses, with some examples focussing on special products such as proteins (enzymes), polysaccharides, polyunsaturated fatty acids and other low molecular weight products. The application of a new membrane adsorber is described as well as the use of solvent extraction in marine biotechnology.

Aquaculture↗

The relevance of pharmacokinetics in the development of biotechnology products.

Biotechnology derived medicines will have an increasing impact not only upon medical practice but also upon the working lives of many pharmaceutical scientists. Whilst such medicines may be viewed as highly sophisticated to the clinician and scientist, the computer will still rightly demand that they are both efficacious and safe. Impacting as it does upon all phases of drug development and facilitating quantitative relationship between administered dose and systemic drug concentration, pharmacokinetics has an important role to play in the development of all medicines. Bioanalysis is an essential prelude to any pharmacokinetic investigation. For many biotechnology products the immunoassay and bioassay methodologies employed are often relatively non-specific and imprecise and yield assay dependent pharmacokinetic parameters. Other factors may also confound the pharmacokinetic evaluation of biotechnological products. In vivo binding proteins (including antibodies) may not only interfere with bioanalytical methodology but also have a significant effect on the pharmacokinetics and biological activity of certain macromolecules. Antibody formation is a particular problem in the preclinical evaluation of human proteins. Unlike most conventional pharmaceuticals, the rate and time of delivery into the systemic circulation is a fundamental component of the biological activity of many biological molecules.

Animals↗

Agricultural biotechnology: myth and measurement.

The title of this article could easily have been "The Inquisition, The First 100 Million Acres, and Future Quantification Challenges." After approximately 20 years of research, transgenic crops have made it through the maze of scientific and regulatory testing to reach commercial practice and have been adopted at a rapid rate. Plant scientists and growers have quickly recognized the extensive portfolio of benefits that can be obtained: it is analogous to the discovery of electricity (biotech) in a world of candles (conventional breeding). Despite the potential for advancement, a few groups have revolted against biotechnological crops. Misinformation has been used to capture headlines, and good scientific research principles have been castigated by self-appointed judges. Conversely, the probiotech camp has not been good at communicating in a broadly understandable manner, but they have the weight of scientific evidence on their side. There are 100 million acres of commercial transgenic crops, and more than 30,000 intensive field trials have been performed, but not one shred of reproducible evidence indicates that biotechnological crops are dangerous, unsafe, or a threat to the environment. Looking ahead, dozens of new beneficial traits are being explored in laboratories across the world, and their application will bring new issues and more difficult challenges. For example, several genes may be stacked in different combinations to provide trait sets with various levels of added value. These crops will no longer be traded as bulk-mixed commodities; therefore, some mechanism will be required to measure the value at each transaction point. The need to develop standards, operating procedures, and devices for accurate, real-time quantification of multiple possible trait sets may be one of the major limitations to rapid future progress in some sectors of crop biotechnology.

Biotechnology↗

Biotechnology in the wood industry.

Wood is a natural, biodegradable and renewable raw material, used in construction and as a feedstock in the paper and wood product industries and in fuel production. Traditionally, biotechnology found little attention in the wood product industries, apart from in paper manufacture. Now, due to growing environmental concern and increasing scientific knowledge, legal restrictions to conventional processes have altered the situation. Biotechnological approaches in the area of wood protection aim at enhancing the treatability of wood with preservatives and replacing chemicals with biological control agents. The substitution of conventional chemical glues in the manufacturing of board materials is achieved through the application of fungal cultures and isolated fungal enzymes. Moreover, biotechnology plays an important role in the waste remediation of preservative-treated waste wood.

Adhesives↗

Knowledge of the Bacillus subtilis genome: impacts on fundamental science and biotechnology.

The advent of genomics has greatly influenced fundamental and applied microbiology. This has become paradigmatic in the case of Bacillus subtilis, a primary model bacterium for research and biotechnology. Indeed, mining its genome has provided more fruitful information than classical approaches would have yielded in a longer period of time. Through advanced analysis of its genome and transcriptome, fundamental discoveries dealing with the informational architecture of the B. subtilis chromosome, as well as with the elucidation of its pathway-level regulation of gene expression, have been achieved. The possibility of performing a complete metabolic manipulation of the secretory pathway of Bacillus is promising important biotechnological fallouts. Similar emphasis exists for the possibility of controlling the cell in the formation of biofilms with specific physical and chemical characteristics. At the theoretical level, the new concept of genetic superinformation has been formulated and its analytical approach implemented, while the understanding of the minimal genetic requirements for the existence of a reproducing bacterial cell is being tackled. In summary, the impact of the B. subtilis genome has philosophically revolutionised the way that basic knowledge is translated into applied microbiology and biotechnology, making this bacterium the workhorse of post-genomic microbiology.

Bacillus subtilis↗

Valuable products from biotechnology of microalgae.

The biotechnology of microalgae has gained considerable importance in recent decades. Applications range from simple biomass production for food and feed to valuable products for ecological applications. For most of these applications, the market is still developing and the biotechnological use of microalgae will extend into new areas. Considering the enormous biodiversity of microalgae and recent developments in genetic engineering, this group of organisms represents one of the most promising sources for new products and applications. With the development of sophisticated culture and screening techniques, microalgal biotechnology can already meet the high demands of both the food and pharmaceutical industries.

Biotechnology↗

Chemicals from biotechnology: molecular plant genetics will challenge the chemical and the fermentation industry.

Industrial biotechnology has evolved as a significant manufacturing tool for products like fuel-grade ethanol, organic acids and bulk amino acids, but most items are still speciality products for food and pharmaceutical applications. Current development projects within the chemical industry, including lactic acid and 1,3-propanediol based polymers and plastics, indicate that new biotechnological processes and products may soon approach the market place, clearly targeted at the leading petrochemical bulk outlets. This is flanked by a strategic shift by the major chemical companies in to "life sciences"-pharmaceuticals, agrochemicals and the seed business as well as biotech fine chemicals. The recent tremendous achievements in molecular plant genetics and transgenic crop breeding will boost agrobiotechnology, agriculture and renewable raw materials as compelling projects for chemistry and biotechnology. New plant-based production routes may challenge established chemical and biochemical domains, but at the same time open new horizons to valuable feedstocks, intermediates and end-products.

Biotechnology↗